Name: Bacillus velezensis CBMB205
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 23706

Type strain:

Species: Bacillus velezensis

Strain Designation: CBMB205

Culture col. no.: DSM 28326, KACC 17006, KACC 13105, NCCB 100236

Strain history: <- KACC; KACC 13105

NCBI tax ID(s): 492670 (species)

SI-ID 371009

KACC 13105, NCCB 100236, LMG 27586

Other strains from Bacillus velezensis

B. velezensis CCUG 50740, CECT 5686, LMG 22478 (type strain)
B. velezensis FZB42, DSM 23117, BGSC 10A6, LMG ...

Section

Bacillus velezensis CBMB205 is an aerobe, spore-forming, mesophilic bacterium that was isolated from rice rhizosphere soil.

Gram-positive
motile
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus velezensis**
	Full Scientific Name (LPSN)	Bacillus velezensis Ruiz-García et al. 2005

	Synonym	Bacillus methylotrophicus
	Synonym	Bacillus amyloliquefaciens subsp. plantarum

Information on morphological and physiological properties Morphology

[Ref.: #31322]	Gram stain	positive
[Ref.: #29542]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #31322]	Cell length	2.5 µm
[Ref.: #29542]	Cell length	1.8-2.7 µm

[Ref.: #31322]	Cell width	0.5 µm
[Ref.: #29542]	Cell width	0.63-0.64 µm

[Ref.: #31322]	Cell shape	rod-shaped
[Ref.: #29542]	Cell shape	rod-shaped

[Ref.: #31322]	Motility	yes
[Ref.: #29542]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %96.65

[Ref.: #29542]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #20434]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #20434]

Culture medium growth

[Ref.: #20434]

Culture medium link

Medium recipe at MediaDive

[Ref.: #20434]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #20434]	growth	30 °C
[Ref.: #29542]	growth	20-45 °C
[Ref.: #29542]	optimum	28 °C
[Ref.: #31322]	growth	15-45 °C
[Ref.: #31322]	optimum	30 °C

[Ref.: #20434]	Temperature range	mesophilic
[Ref.: #29542]	Temperature range	mesophilic
[Ref.: #31322]	Temperature range	mesophilic

		pH
[Ref.: #29542]	optimum	7
[Ref.: #31322]	optimum	7.5
[Ref.: #29542]	growth	02-10
[Ref.: #31322]	growth	05-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29542]

Oxygen tolerance

aerobe

[Ref.: #31322]	Ability of spore formation	yes
[Ref.: #29542]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Salt		Salt conc.
[Ref.: #31322]	NaCl	growth	<12	%
[Ref.: #29542]	NaCl	growth	0-3	%
[Ref.: #29542]	NaCl	optimum	0-3	%

[Ref.: #29542]	Observation	aggregates in chains
[Ref.: #31322]	Observation	aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #29542]	adipate ChEBI	+	carbon source
[Ref.: #31322]	arabinose ChEBI	+	carbon source
[Ref.: #29542]	arabinose ChEBI	+	carbon source
[Ref.: #31322]	cellobiose ChEBI	+	carbon source
[Ref.: #31322]	esculin ChEBI	+	hydrolysis
[Ref.: #29542]	esculin ChEBI	+	hydrolysis
[Ref.: #31322]	fructose ChEBI	+	carbon source
[Ref.: #31322]	glucose ChEBI	+	carbon source
[Ref.: #31322]	glycerol ChEBI	+	carbon source
[Ref.: #31322]	glycogen ChEBI	+	carbon source
[Ref.: #29542]	histidine ChEBI	+	carbon source
[Ref.: #29542]	lactate ChEBI	+	carbon source
[Ref.: #31322]	lactose ChEBI	+	carbon source
[Ref.: #29542]	malate ChEBI	+	carbon source
[Ref.: #31322]	maltose ChEBI	+	carbon source
[Ref.: #31322]	mannitol ChEBI	+	carbon source
[Ref.: #31322]	mannose ChEBI	+	carbon source
[Ref.: #29542]	melibiose ChEBI	+	carbon source
[Ref.: #31322]	nitrate ChEBI	+	reduction
[Ref.: #29542]	nitrate ChEBI	+	reduction
[Ref.: #31322]	raffinose ChEBI	+	carbon source
[Ref.: #29542]	rhamnose ChEBI	+	carbon source
[Ref.: #31322]	ribose ChEBI	+	carbon source
[Ref.: #29542]	ribose ChEBI	+	carbon source
[Ref.: #31322]	salicin ChEBI	+	carbon source
[Ref.: #29542]	salicin ChEBI	+	carbon source
[Ref.: #31322]	sorbitol ChEBI	+	carbon source
[Ref.: #29542]	sorbitol ChEBI	+	carbon source
[Ref.: #31322]	sucrose ChEBI	+	carbon source
[Ref.: #29542]	sucrose ChEBI	+	carbon source
[Ref.: #31322]	trehalose ChEBI	+	carbon source
[Ref.: #31322]	xylose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #31322]		hydrogen sulfide ChEBI	yes

	Enzyme	Enzyme activity	EC number
[Ref.: #31322]	catalase	+	1.11.1.6
[Ref.: #29542]	catalase	+	1.11.1.6
[Ref.: #31322]	cytochrome oxidase	+	1.9.3.1
[Ref.: #29542]	cytochrome oxidase	+	1.9.3.1
[Ref.: #31322]	gelatinase	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #20434]	Sample type/isolated from	rice rhizosphere soil
[Ref.: #20434]	Geographic location (country and/or sea, region)	Chungbuk
[Ref.: #20434]	Country	Republic of Korea
[Ref.: #20434]	Country ISO 3 Code	KOR
[Ref.: #20434]	Continent	Asia
[Ref.: #20434]	Geographic location	36.9667°/127.9500°
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Rhizosphere

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY603658 (>99% sequence identity) for Bacillus from Microbeatlas

Aquatic Samples	2854
Soil Samples	3768
Animal Samples	9674
Plant Samples	1905
Total Samples	18201

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #20434]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20434]	Bacillus methylotrophicus strain CBMB205 16S ribosomal RNA gene, partial sequence	EU194897	1445	ENA	492670 tax ID
[Ref.: #31322]	Bacillus velezensis strain CR-502 16S ribosomal RNA gene, partial sequence	AY603658	1404	GenBank	492670 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bacillus velezensis CBMB205	GCA_002117165	complete	GenBank	492670 tax ID	*
[Ref.: #66792]	Bacillus velezensis CBMB205	GCA_003431885	complete	GenBank	492670 tax ID	*
[Ref.: #66792]	Bacillus velezensis CBMB205	GCA_900100225	scaffold	GenBank	492670 tax ID	*
[Ref.: #66792]	Bacillus methylotrophicus strain KACC 13105	492670.10	wgs	PATRIC	492670 tax ID	*
[Ref.: #66792]	Bacillus velezensis strain CBMB205	492670.316	complete	PATRIC	492670 tax ID	*
[Ref.: #66792]	Bacillus methylotrophicus KACC 13105	2630968484	draft	JGI IMG/M	492670 tax ID	*

[Ref.: #20434]	GC-content	45.0 mol%
[Ref.: #29542]	GC-content	45 mol%
[Ref.: #31322]	GC-content	46.4 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Bacillus velezensis strain CBMB205 PATRIC: 492670.316
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bacillus velezensis CBMB205 NCBI: GCA_002117165.1
[Ref.: #69480]	flagellated	yes	85.113	no
[Ref.: #69480]	gram-positive	yes	91.542	no
[Ref.: #69480]	anaerobic	no	99.287	yes
[Ref.: #69480]	halophile	yes	90.097	yes
[Ref.: #69480]	spore-forming	yes	96.436	no
[Ref.: #69480]	glucose-util	yes	89.473	yes
[Ref.: #69480]	aerobic	yes	87.875	yes
[Ref.: #69480]	thermophile	no	97.027	yes
[Ref.: #69480]	motile	yes	89.838	yes
[Ref.: #69480]	glucose-ferment	no	90.65	no

Availability in culture collections External links

[Ref.: #20434]

Culture collection no.

DSM 28326, KACC 17006, KACC 13105, NCCB 100236

[Ref.: #87752]

SI-ID 371009

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete Genome Sequence of Bacillus velezensis CBMB205, a Phosphate-Solubilizing Bacterium Isolated from the Rhizoplane of Rice in the Republic of Korea.	Hwangbo K, Um Y, Kim KY, Madhaiyan M, Sa TM, Lee Y	Genome Announc	10.1128/genomeA.00654-16	2016	*
Phylogeny	Bacillus oryzicola sp. nov., an Endophytic Bacterium Isolated from the Roots of Rice with Antimicrobial, Plant Growth Promoting, and Systemic Resistance Inducing Activities in Rice.	Chung EJ, Hossain MT, Khan A, Kim KH, Jeon CO, Chung YR	Plant Pathol J	10.5423/PPJ.OA.12.2014.0136	2015	*
Phylogeny	Bacillus methylotrophicus sp. nov., a methanol-utilizing, plant-growth-promoting bacterium isolated from rice rhizosphere soil.	Madhaiyan M, Poonguzhali S, Kwon SW, Sa TM	Int J Syst Evol Microbiol	10.1099/ijs.0.015487-0	2009	*
Phylogeny	Phylogenomic analysis shows that Bacillus amyloliquefaciens subsp. plantarum is a later heterotypic synonym of Bacillus methylotrophicus.	Dunlap CA, Kim SJ, Kwon SW, Rooney AP	Int J Syst Evol Microbiol	10.1099/ijs.0.000226	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20434

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28326

#29542

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25938 (see below)

#31322

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#87752

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID371009.1 )

#25938

IJSEM 2490 2010 ( DOI 10.1099/ijs.0.015487-0 , PubMed 19966000 )

#27637

IJSEM 191 2005 ( DOI 10.1099/ijs.0.63310-0 , PubMed 15653875 )

* These data were automatically processed and therefore are not curated

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Bacillus velezensis strain CBMB205

Bacillus velezensis CBMB205

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