Cellulomonas humilata (DSM 14368, ATCC 25174, CCUG 35596)

Name: Cellulomonas humilata (DSM 14368, ATCC 25174, CCUG 35596)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2359

Type strain

Strain Designation PSU-16S

Culture col. no. DSM 14368 ATCC 25174 CCUG 35596 NRRL B-3752 PSU-16S 4 more

NCBI tax ID(s) 144055

Special Collections DSMZ CCUG JCM CIP

History DSMZ <- CCUG; CCUG 35596 <- NRRL <- Casida, L. E., Jr JCM <-- CIP 104614 <-- ATCC 25174 <-- L. E. Casida, Jr. PSU-16S. CIP <- 1995, ATCC <- L.E. Casida: strain PSU-16S

Links

Cellulomonas humilata PSU-16S is a microaerophile, Gram-positive, rod-shaped bacterium that was isolated from soil.

Gram-positive rod-shaped microaerophile genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 45740

ATCC 25174,CCUG 35596,NRRL B-3752,CIP 104614,NCIMB 10802,JCM 11945,DSM 43390,DSM 14368

@ref 20215

Last LPSN update 2026-05-29 08:25:27

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Cellulomonadaceae

Genus Cellulomonas

Species Cellulomonas humilata

Full scientific name Cellulomonas humilata corrig. (Gledhill and Casida 1969) Collins and Pascual 2000

Synonyms (2)

Actinomyces humiferus
Actinomyces humilatus

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility	Confidence
119987	positive	rod-shaped
125439	positive			97.937

Colony morphology

119987

Hemolysis ability0

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
5324	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
5324	BHI MEDIUM (DSMZ Medium 215)	Medium recipe at MediaDive	Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
38511	MEDIUM 6 - Columbia agar with 10 % horse blood		Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
119987	CIP Medium 6	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
5324	positive	growth	30
38511	positive	growth	30
52846	positive	growth	30-37
67770	positive	growth	30
119987	positive	growth	25-37
119987	negative	growth	10
119987	negative	growth	41
119987	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
5324	microaerophile
5324	aerobe
52846	aerobe
52846	anaerobe
52846	microaerophile
119987	facultative anaerobe
125439	obligate aerobe	99.812

Halophily

@ref	Salt	Growth	Tested relation	Concentration
119987	NaCl	positive	growth	0-10 %

Observation

67770

Observationquinones: MK-9(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
119987	16947 ChEBI	citrate	-	carbon source
119987	4853 ChEBI	esculin	+	hydrolysis
119987	606565 ChEBI	hippurate	+	hydrolysis
119987	17632 ChEBI	nitrate	-	reduction
119987	17632 ChEBI	nitrate	+	respiration
119987	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
119987	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
119987	15688 ChEBI	acetoin	-
119987	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
119987	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
119987	amylase	+
68382	beta-galactosidase	-	3.2.1.23	from API zym
119987	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
119987	caseinase	+	3.4.21.50
119987	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
119987	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
119987	gamma-glutamyltransferase	-	2.3.2.2
119987	gelatinase	-
119987	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
119987	lipase	-
68382	lipase (C 14)	-		from API zym
119987	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
119987	ornithine decarboxylase	-	4.1.1.17
119987	oxidase	-
119987	phenylalanine ammonia-lyase	-	4.3.1.24
68382	trypsin	-	3.4.21.4	from API zym
119987	tryptophan deaminase	-
119987	tween esterase	-
119987	urease	-	3.5.1.5
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
119987	-	+	+	+	-	+	+	-	-	+	+	+	+	-	-	+	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
119987	not determinedn.d.	+	+	+	-	+	-	+	+	+	+	+	+	+	-	-	+	+	-	+	-	+	+	-	+	-	+	-	-	-	-	-	+	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
5324	soil	USA	USA	North America
52846	Soil
67770	Soil	USA	USA	North America
119987	Environment, Soil

Taxonmaps

69479

Global distribution of 16S sequence X82449 (>99% sequence identity) for Cellulomonas from Microbeatlas

Aquatic Samples	607
Soil Samples	3471
Animal Samples	1257
Plant Samples	570
Total Samples	5905

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
5324	1	Risk group (German classification) According to TRBA 466
119987	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM1335977v1 assembly for Cellulomonas humilata ATCC 25174	contig	GCA_013359775	144055.3	2995910351	144055	63.74

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
5324	A.humiferus 16S rRNA gene	X82449	1402		144055

GC content

67770

GC-content (mol%)73

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM1335977v1 assembly for Cellulomonas humilata ATCC 25174
NCBI: GCA_013359775

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.81	no
125439	gram_stain	BacteriaNetⓘ	positive	97.94	no
125439	motility	BacteriaNetⓘ	no	77.46	no
125439	spore_formation	BacteriaNetⓘ	no	76.51	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Cellulomonas humilata strain ATCC 25174
PATRIC: 144055.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	86.79	no
125438	anaerobic	anaerobicⓘ	no	96.07	no
125438	spore-forming	spore-formingⓘ	no	57.85	no
125438	aerobic	aerobicⓘ	yes	76.54	no
125438	thermophilic	thermophileⓘ	no	93.83	yes
125438	flagellated	motile2+ⓘ	no	67.87	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Reclassification of Actinomyces humiferus (Gledhill and Casida) as Cellulomonas humilata nom. corrig., comb. nov.	Collins MD, Pascual C.	Int J Syst Evol Microbiol	10.1099/00207713-50-2-661	2000
Metabolism	Isolation of endophytic bacteria from the leaves of Anredera cordifolia CIX1 for metabolites and their biological activities.	Nxumalo CI, Ngidi LS, Shandu JSE, Maliehe TS.	BMC Complement Med Ther	10.1186/s12906-020-03095-z	2020
	The Pathogenic Role of Actinomyces spp. and Related Organisms in Genitourinary Infections: Discoveries in the New, Modern Diagnostic Era.	Gajdacs M, Urban E.	Antibiotics (Basel)	10.3390/antibiotics9080524	2020
Phylogeny	Cellulomonas aurantiaca sp. nov., isolated from a soil sample from a tangerine field.	Kim SK, Kook M, Yan ZF, Trinh H, Zheng SD, Yang JE, Park SY, Yi TH	Antonie Van Leeuwenhoek	10.1007/s10482-019-01288-2	2019
Phylogeny	Cellulomonas rhizosphaerae sp. nov., a novel actinomycete isolated from soil.	Tian Y, Han C, Hu J, Zhao J, Zhang C, Guo X, Wang X, Xiang W	Int J Syst Evol Microbiol	10.1099/ijsem.0.003258	2019
Phylogeny	Cellulomonas terrae sp. nov., a cellulolytic and xylanolytic bacterium isolated from soil.	An DS, Im WT, Yang HC, Kang MS, Kim KK, Jin L, Kim MK, Lee ST	Int J Syst Evol Microbiol	10.1099/ijs.0.63696-0	2005
Phylogeny	Cellulomonas xylanilytica sp. nov., a cellulolytic and xylanolytic bacterium isolated from a decayed elm tree.	Rivas R, Trujillo ME, Mateos PF, Martinez-Molina E, Velazquez E	Int J Syst Evol Microbiol	10.1099/ijs.0.02866-0	2004

References

#5324	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14368
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#38511	; Curators of the CIP;
#52846	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 35596
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#119987	Collection of Institut Pasteur ; Curators of the CIP; CIP 104614
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Cellulomonas humilata (DSM 14368, ATCC 25174, CCUG 35596)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_013359775

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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