Name: Cellulomonas biazotea 127
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 2350

Type strain:

Species: Cellulomonas biazotea

Strain Designation: 127, NRS-127

Culture col. no.: DSM 20112, ATCC 486, NCDO 1654, NCIB 8077, CCUG 58054, JCM 1340, LMG 16695, CIP 82.11, NCIMB 8077, BCRC 14864, CFBP 4269, CGMCC 1.1899, IAM 12106, IFM 10509, IFO 12680, IMET 10473, KCCM 40760, KCTC 1370, NBRC 12680, NCAIM B.01385, VKM Ac-1410, NCTC 10823

Strain history: CIP <- 1982, ATCC <- N.R. Smith: strain NRS-127

NCBI tax ID(s): 1709 (species)

SI-ID 8217

LMG 16695, ATCC 486, DSM 20112, IAM 12106, IFO 12680, IMET 10473, JCM 1340, KCTC 1370, NCFB 1654, NCIMB 8077, NCIB 8077, NCDO 1654, NCTC 10823, CCRC 14864, VKM Ac-1410, CIP 82.11, AS 1.1899, NBRC 12680, CFBP 4269, NCAIM B.01385, BCRC 14864, NCIM 2550, CGMCC 1.1899, CCUG 58054

Special Collections

Other strains from Cellulomonas biazotea

Section

Cellulomonas biazotea 127 is an aerobe, spore-forming, Gram-positive bacterium of the family Cellulomonadaceae.

Gram-positive
spore-forming
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Cellulomonadaceae*
	Genus	*Cellulomonas*
	Species	**Cellulomonas biazotea**
	Full Scientific Name (LPSN)	Cellulomonas biazotea (Kellerman et al. 1913) Bergey et al. 1923 (Approved Lists 1980)

Synonym

"Bacillus biazoteus"

Information on morphological and physiological properties Morphology

[Ref.: #18418]	Colony color	Ivory (1014)
[Ref.: #18418]	Incubation period	10-14 days
[Ref.: #18418]	Cultivation medium used	ISP 2

[Ref.: #18418]	Colony color	Lemon yellow (1012)
[Ref.: #18418]	Incubation period	10-14 days
[Ref.: #18418]	Cultivation medium used	ISP 3

[Ref.: #18418]	Colony color	Light ivory (1015)
[Ref.: #18418]	Incubation period	10-14 days
[Ref.: #18418]	Cultivation medium used	ISP 4

[Ref.: #18418]	Incubation period	10-14 days
[Ref.: #18418]	Cultivation medium used	ISP 6

[Ref.: #18418]	Multicellular complex forming ability	no
[Ref.: #18418]	Medium Name (multicellularity)	ISP 2

[Ref.: #18418]	Multicellular complex forming ability	no
[Ref.: #18418]	Medium Name (multicellularity)	ISP 3

[Ref.: #18418]	Multicellular complex forming ability	no
[Ref.: #18418]	Medium Name (multicellularity)	ISP 4

[Ref.: #18418]	Multicellular complex forming ability	no
[Ref.: #18418]	Medium Name (multicellularity)	ISP 6

Information on culture and growth conditions Culture and growth conditions

[Ref.: #18418]

Culture medium

ISP 2

[Ref.: #18418]

Culture medium growth

[Ref.: #18418]

Culture medium composition

expand / minimize

[Ref.: #18418]

Culture medium

ISP 3

[Ref.: #18418]

Culture medium growth

[Ref.: #18418]

Culture medium composition

expand / minimize

[Ref.: #18418]

Culture medium

ISP 4

[Ref.: #18418]

Culture medium growth

[Ref.: #18418]

Culture medium composition

expand / minimize

[Ref.: #18418]

Culture medium

ISP 6

[Ref.: #18418]

Culture medium growth

[Ref.: #18418]

Culture medium composition

expand / minimize

[Ref.: #8537]

Culture medium

CORYNEBACTERIUM AGAR (DSMZ Medium 53)

[Ref.: #8537]

Culture medium growth

[Ref.: #8537]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8537]

Culture medium composition

expand / minimize

[Ref.: #37733]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #37733]

Culture medium growth

[Ref.: #37733]

Culture medium composition

expand / minimize

[Ref.: #116800]

Culture medium

CIP Medium 3

[Ref.: #116800]

Culture medium growth

[Ref.: #116800]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #8537]	growth	30 °C
[Ref.: #18418]	optimum	28 °C
[Ref.: #37733]	growth	30 °C
[Ref.: #61575]	growth	30-37 °C
[Ref.: #67770]	growth	28 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #61575]

Oxygen tolerance

aerobe

[Ref.: #69481]

Ability of spore formation

yes Confidence in %94

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #18418]		NaCl		maximum	2.5	%

[Ref.: #8537]	Murein short key	A21.05
[Ref.: #8537]	Murein types	A4ß L-Orn-D-Glu

[Ref.: #67770]

Observation

quinones: MK-9(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #18418]	arabinose ChEBI	-
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #18418]	cellulose ChEBI	-
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68379]	D-glucose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	+	fermentation	* from API Coryne
[Ref.: #18418]	fructose ChEBI	-
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #18418]	glucose ChEBI	+
[Ref.: #68379]	lactose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68379]	maltose ChEBI	+	fermentation	* from API Coryne
[Ref.: #18418]	mannitol ChEBI	-
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #18418]	raffinose ChEBI	+
[Ref.: #18418]	sucrose ChEBI	-
[Ref.: #68379]	sucrose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E, API Coryne
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym, API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym, API Coryne, API 20E
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #68379]	catalase	+	1.11.1.6	* from API Coryne
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym, API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #68379]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API Coryne
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne, API 20E
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #18418]	14	+	-	-	-	-	-	-	-	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #18418]	99	-	-	+	-	+	+	+	+	-	-	+	not determinedn.d.	+	+	-	+	+	+	+	-	not determinedn.d.
[Ref.: #61575]	1764	+	+	+	+	-	+	+	+	+	-	-	-	+	+	+	-	+	+	+	+	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #18418]	128	not determinedn.d.	+	-	+	-	+	+	+	+	-	-	+	+	+	-	+	+	+	-	-
[Ref.: #61575]	2129	-	-	+	-	-	+	-	-	-	-	+	+	+	+	-	+	+	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #61575]	Sample type/isolated from	Soil
[Ref.: #61575]	Geographic location (country and/or sea, region)	UT,Logan,Greenville Agr.Exp.Farm
[Ref.: #61575]	Country	USA
[Ref.: #61575]	Country ISO 3 Code	USA
[Ref.: #61575]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Soil from Greenville Agr. Exp. Farm
[Ref.: #67770]	Geographic location (country and/or sea, region)	Logan, UT
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America

[Ref.: #116800]	Sample type/isolated from	Environment, Soil
[Ref.: #116800]	Geographic location (country and/or sea, region)	Logan, Utah
[Ref.: #116800]	Country	United States of America
[Ref.: #116800]	Country ISO 3 Code	USA
[Ref.: #116800]	Continent	North America

[Ref.: #37733]	Geographic location (country and/or sea, region)	Logan, UT
[Ref.: #37733]	Country	USA
[Ref.: #37733]	Country ISO 3 Code	USA
[Ref.: #37733]	Continent	North America
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence X79462 (>99% sequence identity) for Cellulomonas from Microbeatlas

Aquatic Samples	122
Soil Samples	529
Animal Samples	233
Plant Samples	75
Total Samples	959

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18418]	Biosafety level	1
[Ref.: #8537]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116800]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	C.biazotea DSM 20112 16S rDNA	X79462	1484	GenBank ENA	1709 tax ID	*
[Ref.: #20218]	C.biazotea 16S rRNA gene	X83802	1452	GenBank ENA	1709 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Cellulomonas biazotea NBRC12680	GCA_004306155	scaffold	GenBank ENA	1709 tax ID	*
[Ref.: #66792]	Cellulomonas biazotea strain NBRC12680	1709.6	wgs	PATRIC	1709 tax ID	*
[Ref.: #66792]	Cellulomonas biazotea NBRC12680	2865143354	draft	JGI IMG/M	1709 tax ID	*

[Ref.: #8537]	GC-content	72.2 mol%
[Ref.: #67770]	GC-content	72.2 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Cellulomonas biazotea NBRC12680 NCBI: GCA_004306155.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	94	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Cellulomonas biazotea NBRC12680 NCBI: GCA_004306155.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	86.635	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	95.279	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	69.826	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	64.017	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	97	no
[Ref.: #69480]	flagellated	motile2+ⓘ	no	66.5	no

Availability in culture collections External links

[Ref.: #8537]

Culture collection no.

DSM 20112, ATCC 486, NCDO 1654, NCIB 8077, CCUG 58054, JCM 1340, LMG 16695, CIP 82.11, NCIMB 8077, BCRC 14864, CFBP 4269, CGMCC 1.1899, IAM 12106, IFM 10509, IFO 12680, IMET 10473, KCCM 40760, KCTC 1370, NBRC 12680, NCAIM B.01385, VKM Ac-1410, NCTC 10823

[Ref.: #71905]

SI-ID 8217

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Cellulomonas aurantiaca sp. nov., isolated from a soil sample from a tangerine field.	Kim SK, Kook M, Yan ZF, Trinh H, Zheng SD, Yang JE, Park SY, Yi TH	Antonie Van Leeuwenhoek	10.1007/s10482-019-01288-2	2019	*
Phylogeny	Cellulomonas phragmiteti sp. nov., a cellulolytic bacterium isolated from reed (Phragmites australis) periphyton in a shallow soda pond.	Rusznyak A, Toth EM, Schumann P, Sproer C, Makk J, Szabo G, Vladar P, Marialigeti K, Borsodi AK	Int J Syst Evol Microbiol	10.1099/ijs.0.022608-0	2010	*
Phylogeny	Cellulomonas aerilata sp. nov., isolated from an air sample.	Lee CM, Weon HY, Hong SB, Jeon YA, Schumann P, Kroppenstedt RM, Kwon SW, Stackebrandt E	Int J Syst Evol Microbiol	10.1099/ijs.0.2008/002253-0	2008	*
Phylogeny	Cellulomonas chitinilytica sp. nov., a chitinolytic bacterium isolated from cattle-farm compost.	Yoon MH, Ten LN, Im WT, Lee ST	Int J Syst Evol Microbiol	10.1099/ijs.0.64768-0	2008	*
	Structure of the principal carotenoid pigment of Cellulomonas biazotea.	Weeks OB, Montes AR, Andrewes AG	J Bacteriol	10.1128/jb.141.3.1272-1278.1980	1980	*

References

#8537

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20112

#18418

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#37733 ; Curators of the CIP;
#61575 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 58054

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#71905

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID8217.1 )

#116800 Collection of Institut Pasteur ; Curators of the CIP; CIP 82.11
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Cellulomonas biazotea in external resources:
SILVA
BRENDA
PANGAEA
GBIF

Cellulomonas biazotea NBRC12680

Cellulomonas biazotea NBRC12680

Change proposal

Change proposal