Name: Brevibacillus nitrificans DA2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 23172

Type strain:

Species: Brevibacillus nitrificans

Strain Designation: DA2

Culture col. no.: DSM 26674, JCM 15774, NCIMB 14531

Strain history: I. Yumoto DA2.

NCBI tax ID(s): 651560 (species)

SI-ID 404140

DSM 26674

Section

Brevibacillus nitrificans DA2 is a facultative anaerobe, spore-forming, mesophilic bacterium that was isolated from microbiological agent for enhancing microbial digestion in sewage treatment tanks.

Gram-positive
motile
rod-shaped
spore-forming
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Brevibacillus*
	Species	**Brevibacillus nitrificans**
	Full Scientific Name (LPSN)	Brevibacillus nitrificans Takebe et al. 2012

Information on morphological and physiological properties Morphology

[Ref.: #30280]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #30280]	Cell length	2.5 µm

[Ref.: #30280]	Cell width	0.6 µm

[Ref.: #30280]	Cell shape	rod-shaped

[Ref.: #30280]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %96.859

[Ref.: #30280]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #19166]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #19166]

Culture medium growth

[Ref.: #19166]

Culture medium link

Medium recipe at MediaDive

[Ref.: #19166]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #19166]	growth	30 °C
[Ref.: #30280]	growth	16-44 °C
[Ref.: #30280]	optimum	38 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #19166]	Temperature range	mesophilic
[Ref.: #30280]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #30280]			optimum	7
[Ref.: #30280]			growth	05-08

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30280]

Oxygen tolerance

facultative anaerobe

[Ref.: #30280]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30280]		NaCl		growth	<3	%

[Ref.: #67770]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30280]	esculin ChEBI	+	hydrolysis
[Ref.: #30280]	glucose ChEBI	+	carbon source
[Ref.: #30280]	maltose ChEBI	+	carbon source
[Ref.: #30280]	mannitol ChEBI	+	carbon source
[Ref.: #30280]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #30280]	nitrate ChEBI	+	reduction
[Ref.: #30280]	sucrose ChEBI	+	carbon source
[Ref.: #30280]	trehalose ChEBI	+	carbon source

	Enzyme	Enzyme activity	EC number
[Ref.: #30280]	alkaline phosphatase	+	3.1.3.1
[Ref.: #30280]	catalase	+	1.11.1.6
[Ref.: #30280]	gelatinase	+
[Ref.: #30280]	urease	+	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #19166]	Sample type/isolated from	microbiological agent for enhancing microbial digestion in sewage treatment tanks
[Ref.: #19166]	Country	Japan
[Ref.: #19166]	Country ISO 3 Code	JPN
[Ref.: #19166]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Microbiological agent for enhancing microbial digestion in sewage treatment tanks
* marker position based on {}

Isolation sources categories

#Engineered

#Built environment

#Container (Reservoir)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB507254 (>99% sequence identity) for Brevibacillus from Microbeatlas

Aquatic Samples	125
Soil Samples	355
Animal Samples	163
Plant Samples	89
Total Samples	732

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #19166]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #19166]	Brevibacillus nitrificans gene for 16S ribosomal RNA, partial sequence	AB507254	1491	ENA	651560 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Brevibacillus nitrificans JCM 15774	GCA_003710965	contig	GenBank	651560 tax ID
[Ref.: #66792]	Brevibacillus nitrificans strain JCM 15774	651560.4	wgs	PATRIC	651560 tax ID	*
[Ref.: #66792]	Brevibacillus nitrificans JCM 15774	2851175764	draft	JGI IMG/M	651560 tax ID	*

[Ref.: #19166]	GC-content	54.1 mol%
[Ref.: #67770]	GC-content	54.1 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Brevibacillus nitrificans strain JCM 15774 PATRIC: 651560.4
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Brevibacillus nitrificans strain JCM 15774 PATRIC: 651560.4
[Ref.: #69480]	gram-positive	yes	83.562	yes
[Ref.: #69480]	anaerobic	no	98.18	yes
[Ref.: #69480]	halophile	no	92.166	no
[Ref.: #69480]	spore-forming	yes	94.261	yes
[Ref.: #69480]	glucose-util	yes	88.284	yes
[Ref.: #69480]	thermophile	no	97.82	yes
[Ref.: #69480]	flagellated	yes	66.536	no
[Ref.: #69480]	aerobic	yes	80.706	yes
[Ref.: #69480]	motile	yes	88.532	no
[Ref.: #69480]	glucose-ferment	no	93.037	no

Availability in culture collections External links

[Ref.: #19166]

Culture collection no.

DSM 26674, JCM 15774, NCIMB 14531

[Ref.: #87405]

SI-ID 404140

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Brevibacillus nitrificans sp. nov., a nitrifying bacterium isolated from a microbiological agent for enhancing microbial digestion in sewage treatment tanks.	Takebe F, Hirota K, Nodasaka Y, Yumoto I	Int J Syst Evol Microbiol	10.1099/ijs.0.032342-0	2011	*

References

#19166

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 26674

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30280

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26621 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#87405

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404140.1 )

#26621

IJSEM 2121 2012 ( DOI 10.1099/ijs.0.032342-0 , PubMed 22039003 )

* These data were automatically processed and therefore are not curated

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Brevibacillus nitrificans strain JCM 15774

Brevibacillus nitrificans strain JCM 15774

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