Name: Rhodococcus cerastii C5
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 23161

Type strain:

Species: Rhodococcus cerastii

Strain Designation: C5, C5(2010)

Culture col. no.: DSM 45579, CCM 7906, LMG 26203, CCUG 60725

Strain history: <- P. Kämpfer, Universität Giessen; C5 <- S.A. Wellner

NCBI tax ID(s): 908616 (species)

SI-ID 375955

LMG 26203, CCM 7906, DSM 45579, CCUG 60725

Special Collections

Section

Rhodococcus cerastii C5 is an aerobe, Gram-positive, rod-shaped prokaryote that was isolated from leaf surface of Cerastium holosteoides.

Gram-positive
rod-shaped
aerobe
16S sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Genus	*Rhodococcus*
	Species	**Rhodococcus cerastii**
	Full Scientific Name (LPSN)	Rhodococcus cerastii Kämpfer et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #30808]	Gram stain	positive

[Ref.: #30808]	Cell length	2.5 µm

[Ref.: #30808]	Cell width	0.9 µm

[Ref.: #30808]	Cell shape	rod-shaped

[Ref.: #30808]	Motility	no

[Ref.: #69407]	Colony color	Daffodil yellow (1007)
[Ref.: #69407]	Cultivation medium used	suter with tyrosine

[Ref.: #69407]	Colony color	Maize yellow (1006)
[Ref.: #69407]	Cultivation medium used	ISP 2

[Ref.: #69407]	Colony color	Maize yellow (1006)
[Ref.: #69407]	Cultivation medium used	ISP 5

[Ref.: #69407]	Colony color	Maize yellow (1006)
[Ref.: #69407]	Cultivation medium used	ISP 7

[Ref.: #69407]	Colony color	Maize yellow (1006)
[Ref.: #69407]	Cultivation medium used	suter without tyrosine

[Ref.: #62415]	Incubation period	2 days

[Ref.: #30808]	Pigment production	yes
[Ref.: #69407]	Pigment production	no
[Ref.: #69407]	Pigment name	Melanin
[Ref.: #69407]	Pigment production	no
[Ref.: #69407]	Pigment name	soluble pigment

[Ref.: #69407]	Multicellular complex forming ability	no
[Ref.: #69407]	Multicellular complex name	Aerial mycelium
[Ref.: #69407]	Medium Name (multicellularity)	ISP 2

[Ref.: #69407]	Multicellular complex forming ability	no
[Ref.: #69407]	Multicellular complex name	Aerial mycelium
[Ref.: #69407]	Medium Name (multicellularity)	ISP 5

[Ref.: #69407]	Multicellular complex forming ability	no
[Ref.: #69407]	Multicellular complex name	Aerial mycelium
[Ref.: #69407]	Medium Name (multicellularity)	ISP 7

[Ref.: #69407]	Multicellular complex forming ability	no
[Ref.: #69407]	Multicellular complex name	Aerial mycelium
[Ref.: #69407]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69407]	Multicellular complex forming ability	no
[Ref.: #69407]	Multicellular complex name	Aerial mycelium
[Ref.: #69407]	Medium Name (multicellularity)	suter without tyrosine

Multimedia content

[Ref.: #19257]

Caption:

Medium 215 28°C

[Ref.: #19257]

Intellectual property rights:

[Ref.: #69407]

Caption:

Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69407]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

[Ref.: #69407]

Caption:

Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69407]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

Information on culture and growth conditions Culture and growth conditions

[Ref.: #19257]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #19257]

Culture medium growth

[Ref.: #19257]

Culture medium link

Medium recipe at MediaDive

[Ref.: #19257]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #19257]	growth	28 °C
[Ref.: #30808]	growth	15-50 °C
[Ref.: #30808]	optimum	30 °C
[Ref.: #62415]	growth	30 °C

	pH	Kind of pH		pH
[Ref.: #30808]			optimum	7.5
[Ref.: #30808]			growth	6.5-10.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30808]	Oxygen tolerance	aerobe
[Ref.: #62415]	Oxygen tolerance	aerobe

[Ref.: #30808]

Ability of spore formation

	Salt		Salt conc.
[Ref.: #30808]	NaCl	growth	01-06	%
[Ref.: #30808]	NaCl	optimum	2.5	%
[Ref.: #69407]	NaCl	growth	0-5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30808]	arabinose ChEBI	+	carbon source
[Ref.: #69407]	arabinose ChEBI	-	growth
[Ref.: #69407]	cellulose ChEBI	-	growth
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #30808]	esculin ChEBI	+	hydrolysis
[Ref.: #69407]	fructose ChEBI	-	growth
[Ref.: #30808]	galactose ChEBI	+	carbon source
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69407]	glucose ChEBI	-	growth
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #69407]	inositol ChEBI	-	growth
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #30808]	leucine ChEBI	+	carbon source
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69407]	mannose ChEBI	-	growth
[Ref.: #30808]	melibiose ChEBI	+	carbon source
[Ref.: #30808]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #30808]	phenylacetate ChEBI	+	carbon source
[Ref.: #30808]	putrescine ChEBI	+	carbon source
[Ref.: #30808]	pyruvate ChEBI	+	carbon source
[Ref.: #69407]	raffinose ChEBI	-	growth
[Ref.: #69407]	rhamnose ChEBI	-	growth
[Ref.: #30808]	ribose ChEBI	+	carbon source
[Ref.: #30808]	salicin ChEBI	+	carbon source
[Ref.: #30808]	sorbitol ChEBI	+	carbon source
[Ref.: #69407]	sucrose ChEBI	-	growth
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #30808]	trehalose ChEBI	+	carbon source
[Ref.: #68379]	urea ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #69407]	xylose ChEBI	-	growth
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne, API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68379]	beta-galactosidase	+	3.2.1.23	* from API Coryne, API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne, API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68379]	urease	+	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69407]	3900	-	-	-	-	-	+	+	-	+/-	+	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69407]	6084	not determinedn.d.	+/-	+/-	+	+/-	+	+	+/-	+/-	-	+/-	+/-	+	+	+/-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #19257]	Sample type/isolated from	leaf surface of Cerastium holosteoides
[Ref.: #19257]	Host species	Cerastium holosteoides
[Ref.: #19257]	Geographic location (country and/or sea, region)	Thuringia, Hainich-Dün region
[Ref.: #19257]	Country	Germany
[Ref.: #19257]	Country ISO 3 Code	DEU
[Ref.: #19257]	Continent	Europe

[Ref.: #62415]	Sample type/isolated from	Leaf,Cerastium holosteoides
[Ref.: #62415]	Sampling date	2008
[Ref.: #62415]	Geographic location (country and/or sea, region)	Hainich-Dün (Thüringen)
[Ref.: #62415]	Country	Germany
[Ref.: #62415]	Country ISO 3 Code	DEU
[Ref.: #62415]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Leaf (Phyllosphere)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence FR714842 (>99% sequence identity) for Rhodococcus from Microbeatlas

Aquatic Samples	1392
Soil Samples	337
Animal Samples	1539
Plant Samples	167
Total Samples	3435

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #19257]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #19257]	Rhodococcus cerastii partial 16S rRNA gene, type strain C5T	FR714842	1337	GenBank ENA	908616 tax ID

Availability in culture collections External links

[Ref.: #19257]

Culture collection no.

DSM 45579, CCM 7906, LMG 26203, CCUG 60725

[Ref.: #87395]

SI-ID 375955

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Rhodococcus cerastii sp. nov. and Rhodococcus trifolii sp. nov., two novel species isolated from leaf surfaces.	Kampfer P, Wellner S, Lohse K, Lodders N, Martin K	Int J Syst Evol Microbiol	10.1099/ijs.0.044958-0	2012	*

References

#19257

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45579

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30808

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27139 (see below)

#62415 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 60725

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69407

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#87395

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID375955.1 )

#27139

IJSEM 1024 2013 ( DOI 10.1099/ijs.0.044958-0 , PubMed 22685110 )

* These data were automatically processed and therefore are not curated

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