Methylopila musalis (DSM 24986, CCUG 61696, VKM B-2646)

Name: Methylopila musalis (DSM 24986, CCUG 61696, VKM B-2646)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 23093

Type strain

Strain Designation MUSA

Culture col. no. DSM 24986 CCUG 61696 VKM B-2646 MUSA

NCBI tax ID(s) 1134781

Special Collections DSMZ CCUG Literature strains

History DSMZ <- N. V. Doronina, Inst. Biochem. Physiol. Microorg., RAS, Pushchino, Russia; MUSA

Links

Methylopila musalis MUSA is an aerobe, Gram-negative, motile bacterium that was isolated from banana fruit of Musa paradisiaca var. sapientum.

Gram-negative motile rod-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 402053

VKM B-2646,DSM 24986,CCUG 61696

@ref 20215

Last LPSN update 2026-05-29 08:30:22

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Hyphomicrobiales

Family Methylopilaceae

Genus Methylopila

Species Methylopila musalis

Full scientific name Methylopila musalis Doronina et al. 2013

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
30687	negative	1.75 µm	0.65 µm	rod-shaped

Colony morphology

62695

Incubation period2-3 days

Pigmentation

30687

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
19321	MEDIUM K (DSMZ Medium 1122)		Medium recipe at MediaDive	Name: MEDIUM K (DSMZ Medium 1122) Composition: Agar 20.0 g/l KH2PO4 2.0 g/l (NH4)2SO4 2.0 g/l NaCl 0.5 g/l MgSO4 x 7 H2O 0.125 g/l FeSO4 x 7 H2O 0.002 g/l Methanol Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
19321	positive	growth	28
30687	positive	growth	16-40
30687	positive	optimum	29
62695	positive	growth	30

Culture pH

@ref	Ability	Type	PH	PH range
30687	positive	optimum	7
30687	positive	growth	5.5-9.5	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
30687	aerobe
62695	aerobe
125439	obligate aerobe	98.04

Spore formation

@ref	Spore formation	Confidence
30687
125439		98.71

Halophily

@ref	Salt	Growth	Tested relation	Concentration
30687	NaCl	positive	growth	0-2.5 %
30687	NaCl	positive	optimum	0.5 %

Observation

30687

Observationaggregates in clumps

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
30687	28757 ChEBI	fructose	+	carbon source
30687	17234 ChEBI	glucose	+	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
30687	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
30687	35581 ChEBI	indole	+

Enzymes

@ref	Value	Activity	Ec
30687	catalase	+	1.11.1.6
30687	cytochrome oxidase	+	1.9.3.1
30687	urease	+	3.5.1.5

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Fruit (Seed)

Isolation

@ref	Sample type	Host species	Country	Country ISO 3 Code	Continent	Sampling date
19321	banana fruit of Musa paradisiaca var. sapientum	Musa paradisiaca var. sapientum	Ecuador	ECU	Middle and South America
62695	Banana		Ecuador	ECU	Middle and South America	2009

Taxonmaps

69479

Global distribution of 16S sequence JQ173144 (>99% sequence identity) for Methylopila from Microbeatlas

Aquatic Samples	2
Soil Samples	1
Animal Samples	8
Plant Samples	2
Total Samples	13

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
19321	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM4267972v1 assembly for Methylopila musalis CCUG 61696	scaffold	GCA_042679725	1134781	0

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
19321	Methylopila musalis strain MUSA 16S ribosomal RNA gene, partial sequence	JQ173144	1307		1134781

GC content

@ref	GC-content (mol%)	Method
19321	64.5	thermal denaturation, midpoint method (Tm)
30687	64.5

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM4267972v1 assembly for Methylopila musalis CCUG 61696
NCBI: GCA_042679725

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.04	no
125439	gram_stain	BacteriaNetⓘ	negative	80.28	no
125439	motility	BacteriaNetⓘ	yes	56.57	no
125439	spore_formation	BacteriaNetⓘ	no	98.71	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methylopila musalis sp. nov., an aerobic, facultatively methylotrophic bacterium isolated from banana fruit.	Doronina NV, Kaparullina EN, Bykova TV, Trotsenko YA	Int J Syst Evol Microbiol	10.1099/ijs.0.042028-0	2012

References

#19321	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 24986
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#27018	IJSEM 1847 2013 ( DOI 10.1099/ijs.0.042028-0 , PubMed 22984139 )
#30687	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27018
#62695	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 61696
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methylopila musalis (DSM 24986, CCUG 61696, VKM B-2646)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_042679725

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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