Name: Methylopila musalis MUSA
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 23093

Type strain:

Species: Methylopila musalis

Strain Designation: MUSA

Culture col. no.: DSM 24986, CCUG 61696, VKM B-2646

Strain history: <- N. V. Doronina, Inst. Biochem. Physiol. Microorg., RAS, Pushchino, Russia; MUSA

NCBI tax ID(s): 1134781 (species)

SI-ID 402053

DSM 24986, CCUG 61696

Section

Methylopila musalis MUSA is an aerobe, mesophilic, Gram-negative bacterium that was isolated from banana fruit of Musa paradisiaca var. sapientum.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Methylocystaceae*
	Genus	*Methylopila*
	Species	**Methylopila musalis**
	Full Scientific Name (LPSN)	Methylopila musalis Doronina et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #30687]	Gram stain	negative

[Ref.: #30687]	Cell length	1.75 µm

[Ref.: #30687]	Cell width	0.65 µm

[Ref.: #30687]	Cell shape	rod-shaped

[Ref.: #30687]	Motility	yes

[Ref.: #62695]

Incubation period

2-3 days

[Ref.: #30687]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #19321]

Culture medium

MEDIUM K (DSMZ Medium 1122)

[Ref.: #19321]

Culture medium growth

[Ref.: #19321]

Culture medium link

Medium recipe at MediaDive

[Ref.: #19321]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #19321]	growth	28 °C
[Ref.: #30687]	growth	16-40 °C
[Ref.: #30687]	optimum	29 °C
[Ref.: #62695]	growth	30 °C

[Ref.: #19321]	Temperature range	mesophilic
[Ref.: #30687]	Temperature range	mesophilic
[Ref.: #62695]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #30687]			optimum	7
[Ref.: #30687]			growth	5.5-9.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30687]	Oxygen tolerance	aerobe
[Ref.: #62695]	Oxygen tolerance	aerobe

[Ref.: #30687]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30687]		NaCl		growth	0-2.5	%
[Ref.: #30687]		NaCl		optimum	0.5	%

[Ref.: #30687]

Observation

aggregates in clumps

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30687]		fructose ChEBI	+	carbon source
[Ref.: #30687]		glucose ChEBI	+	carbon source

	Metabolite production	Metabolite	Production
[Ref.: #30687]		indole ChEBI	yes

	Physiological tests	Metabolite	Indole test
[Ref.: #30687]		indole ChEBI	+

	Enzyme	Enzyme activity	EC number
[Ref.: #30687]	catalase	+	1.11.1.6
[Ref.: #30687]	cytochrome oxidase	+	1.9.3.1
[Ref.: #30687]	urease	+	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #19321]	Sample type/isolated from	banana fruit of Musa paradisiaca var. sapientum
[Ref.: #19321]	Host species	Musa paradisiaca var. sapientum
[Ref.: #19321]	Country	Ecuador
[Ref.: #19321]	Country ISO 3 Code	ECU
[Ref.: #19321]	Continent	Middle and South America

[Ref.: #62695]	Sample type/isolated from	Banana
[Ref.: #62695]	Sampling date	2009
[Ref.: #62695]	Country	Ecuador
[Ref.: #62695]	Country ISO 3 Code	ECU
[Ref.: #62695]	Continent	Middle and South America
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Fruit (Seed)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JQ173144 (>99% sequence identity) for Methylopila from Microbeatlas

Aquatic Samples	2
Soil Samples	1
Animal Samples	8
Plant Samples	2
Total Samples	13

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #19321]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #19321]	Methylopila musalis strain MUSA 16S ribosomal RNA gene, partial sequence	JQ173144	1307	ENA	1134781 tax ID

[Ref.: #19321]	GC-content	64.5 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #30687]	GC-content	64.5 mol%

Availability in culture collections External links

[Ref.: #19321]

Culture collection no.

DSM 24986, CCUG 61696, VKM B-2646

[Ref.: #87331]

SI-ID 402053

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methylopila musalis sp. nov., an aerobic, facultatively methylotrophic bacterium isolated from banana fruit.	Doronina NV, Kaparullina EN, Bykova TV, Trotsenko YA	Int J Syst Evol Microbiol	10.1099/ijs.0.042028-0	2012	*

References

#19321

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 24986

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30687

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27018 (see below)

#62695 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 61696

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#87331

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID402053.1 )

#27018

IJSEM 1847 2013 ( DOI 10.1099/ijs.0.042028-0 , PubMed 22984139 )

* These data were automatically processed and therefore are not curated

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