Klebsiella michiganensis (DSM 25444, ATCC BAA-2403, CIP 110787)

Name: Klebsiella michiganensis (DSM 25444, ATCC BAA-2403, CIP 110787)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 23002

Type strain

Strain Designation W14

Culture col. no. DSM 25444 ATCC BAA-2403 CIP 110787 CCUG 66515 W14

NCBI tax ID(s) 1134687

Special Collections DSMZ CCUG CIP

History DSMZ <- R. Saha, NSF Int., Ann Arbor, USA; W14 CIP <- 2014, DSMZ <- R. Saha, NSF Int., Ann Arbor, Michigan, USA: strain W14

Links

Klebsiella michiganensis W14 is an aerobe, Gram-negative, rod-shaped bacterium that was isolated from tooth brush holder.

Gram-negative rod-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 398220

LMG 30655,ATCC BAA-2403,DSM 25444,CIP 110787,CCUG 66515T

@ref 20215

Last LPSN update 2026-05-29 08:30:21

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Enterobacterales

Family Enterobacteriaceae

Genus Klebsiella

Species Klebsiella michiganensis

Full scientific name Klebsiella michiganensis Saha et al. 2013

BacDive ID	Other strains from **Klebsiella michiganensis** (6)	Type strain
132136	K. michiganensis H1g, DSM 103279
136235	K. michiganensis CIP 53.16, BR 32
156589	K. michiganensis CCUG 63157 A
156590	K. michiganensis CCUG 63157 B
156889	K. michiganensis CCUG 66352
176933	K. michiganensis JN_Kp126, DSM 117135

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
125439	negative		92.504
125438	negative		100
120943	negative	rod-shaped

Multimedia

@ref:	19209
multimedia content:	DSM_25444.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_25444.jpg
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
36269	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
19209	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
19209	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)	Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
19209	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
120943	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
19209	positive	growth	37
36269	positive	growth	30
63343	positive	growth	30-37

Physiology and metabolism

Oxygen tolerance

63343

Oxygen toleranceaerobe

Spore formation

@ref	Spore formation	Confidence
125439		98.102

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68368	27613 ChEBI	amygdalin	+	fermentation	from API 20E
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
68368	29016 ChEBI	arginine	-	hydrolysis	from API 20E
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	+	fermentation	from API 20NE
68368	17634 ChEBI	D-glucose	+	fermentation	from API 20E
68369	16899 ChEBI	D-mannitol	+	assimilation	from API 20NE
68368	16899 ChEBI	D-mannitol	+	fermentation	from API 20E
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
68369	4853 ChEBI	esculin	+	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68368	5291 ChEBI	gelatin	-	hydrolysis	from API 20E
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
68368	30849 ChEBI	L-arabinose	+	fermentation	from API 20E
68368	62345 ChEBI	L-rhamnose	+	fermentation	from API 20E
68368	25094 ChEBI	lysine	+	degradation	from API 20E
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	+	assimilation	from API 20NE
68368	28053 ChEBI	melibiose	+	fermentation	from API 20E
68368	17268 ChEBI	myo-inositol	+	fermentation	from API 20E
68369	59640 ChEBI	N-acetylglucosamine	+	assimilation	from API 20NE
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
68368	30911 ChEBI	sorbitol	+	fermentation	from API 20E
68368	17992 ChEBI	sucrose	+	fermentation	from API 20E
68369	27897 ChEBI	tryptophan	+	energy source	from API 20NE
68368	27897 ChEBI	tryptophan	+	energy source	from API 20E
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
68369	35581 ChEBI	indole	from API 20NE
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	+		from API 20E
68369	35581 ChEBI	indole		+	from API 20NE
68368	35581 ChEBI	indole		+	from API 20E

Enzymes

@ref	Value	Activity	Ec
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68369	beta-glucosidase	+	3.2.1.21	from API 20NE
68369	cytochrome oxidase	-	1.9.3.1	from API 20NE
68368	cytochrome oxidase	-	1.9.3.1	from API 20E
68369	gelatinase	-		from API 20NE
68368	gelatinase	-		from API 20E
68368	lysine decarboxylase	+	4.1.1.18	from API 20E
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68369	urease	-	3.5.1.5	from API 20NE
68368	urease	-	3.5.1.5	from API 20E

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
19209	+	-	+	-	+	-	-	-	+	+	-	+	+	+	+	+	+	+	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
19209	+	+	+	-	-	+	-	+	+	+	+	+	+	+	+	-	-	+	+	+	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Industrial	#Engineered product

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
19209	tooth brush holder	Ann Arbor, MI	USA	USA	North America
63343	Tooth brush holder	Ann Arbor,MI	USA	USA	North America
120943	Tooth brush holder	Ann Arbor, Michigan	United States of America	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence JQ070300 (>99% sequence identity) for Enterobacteriaceae from Microbeatlas

Aquatic Samples	233
Soil Samples	121
Animal Samples	3440
Plant Samples	216
Total Samples	4010

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
120943	2	Risk group (French classification) According to TRBA 466
19209	2	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM292590v1 assembly for Klebsiella michiganensis DSM 25444	scaffold	GCA_002925905	1134687.61	2916627578	1134687	71.89
66792	ASM917348v1 assembly for Klebsiella michiganensis CCUG 66515	contig	GCA_009173485	1134687.164		1134687	31.55

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
19209	Klebsiella michiganensis strain W14 16S ribosomal RNA gene, partial sequence	JQ070300	1395		1134687

GC content

@ref	GC-content (mol%)	Method
19209	54.6	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM292590v1 assembly for Klebsiella michiganensis DSM 25444
NCBI: GCA_002925905

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	aerobe	83.36	no
125439	gram_stain	BacteriaNetⓘ	negative	92.50	no
125439	motility	BacteriaNetⓘ	yes	66.31	no
125439	spore_formation	BacteriaNetⓘ	no	98.10	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Klebsiella michiganensis DSM 25444
NCBI: GCA_002925905.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	100.00	no
125438	anaerobic	anaerobicⓘ	no	98.20	no
125438	aerobic	aerobicⓘ	yes	53.24	yes
125438	spore-forming	spore-formingⓘ	no	88.91	no
125438	thermophilic	thermophileⓘ	no	99.25	yes
125438	flagellated	motile2+ⓘ	no	75.27	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Characterization of a Highly Virulent Klebsiella michiganensis Strain Isolated from a Preterm Infant with Sepsis.	Xu P, Zhang D, Zhuo W, Zhou L, Du Y, Zhang P, Ma L, Wang Y.	Infect Drug Resist	10.2147/idr.s481750	2024
	Klebsiella oxytoca Complex: Update on Taxonomy, Antimicrobial Resistance, and Virulence.	Yang J, Long H, Hu Y, Feng Y, McNally A, Zong Z.	Clin Microbiol Rev	10.1128/cmr.00006-21	2022
Metabolism	Gut microbiota promotes host resistance to low-temperature stress by stimulating its arginine and proline metabolism pathway in adult Bactrocera dorsalis.	Raza MF, Wang Y, Cai Z, Bai S, Yao Z, Awan UA, Zhang Z, Zheng W, Zhang H.	PLoS Pathog	10.1371/journal.ppat.1008441	2020
Metabolism	Characterized non-transient microbiota from stinkbug (Nezara viridula) midgut deactivates soybean chemical defenses.	Medina V, Sardoy PM, Soria M, Vay CA, Gutkind GO, Zavala JA.	PLoS One	10.1371/journal.pone.0200161	2018
	KpSC-ID: a multiplex real-time PCR assay for the simultaneous detection of the Klebsiella pneumoniae species complex and specific identification of Klebsiella pneumoniae, Klebsiella quasipneumoniae and Klebsiella variicola.	McAndrew G, Barbier E, Rodrigues C, Piveteau P, Brisse S, Reddington K.	Microbiology (Reading)	10.1099/mic.0.001587	2025
Phylogeny	Description of Klebsiella spallanzanii sp. nov. and of Klebsiella pasteurii sp. nov.	Merla C, Rodrigues C, Passet V, Corbella M, Thorpe HA, Kallonen TVS, Zong Z, Marone P, Bandi C, Sassera D, Corander J, Feil EJ, Brisse S.	Front Microbiol	10.3389/fmicb.2019.02360	2019
Phylogeny	Klebsiella indica sp. nov., isolated from the surface of a tomato.	Gujarati S, Chaudhari D, Hagir A, Khairnar M, Shouche Y, Rahi P	Int J Syst Evol Microbiol	10.1099/ijsem.0.004168	2020
Phylogeny	Klebsiella michiganensis sp. nov., a new bacterium isolated from a tooth brush holder.	Saha R, Farrance CE, Verghese B, Hong S, Donofrio RS	Curr Microbiol	10.1007/s00284-012-0245-x	2012

References

#19209	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25444
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#36269	; Curators of the CIP;
#63343	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 66515
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68368	Automatically annotated from API 20E .
#68369	Automatically annotated from API 20NE .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120943	Collection of Institut Pasteur ; Curators of the CIP; CIP 110787
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Klebsiella michiganensis (DSM 25444, ATCC BAA-2403, CIP 110787)

Name and taxonomic classification

Morphology

Cell morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002925905

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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