Klebsiella michiganensis W14 | Type strain | DSM 25444, ATCC BAA-2403, CIP 110787, CCUG 66515

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Information on culture and growth conditions Culture and growth conditions

[Ref.: #36269]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #36269]

Culture medium growth

[Ref.: #36269]

Culture medium composition

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

Culture medium growth

Medium recipe at MediaDive

Culture medium link

Culture medium composition

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

Culture medium growth

Medium recipe at MediaDive

Culture medium link

Culture medium composition

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

Culture medium growth

Medium recipe at MediaDive

Culture medium link

Culture medium composition

Culture medium

CIP Medium 72

Culture medium growth

Medium recipe provided by DSMZ

Culture medium link

		Temperature
[Ref.: #19209]	growth	37 °C
[Ref.: #36269]	growth	30 °C
[Ref.: #63343]	growth	30-37 °C

[Ref.: #19209]	Temperature range	mesophilic
[Ref.: #36269]	Temperature range	mesophilic
[Ref.: #63343]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #63343]

Oxygen tolerance

aerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68368]	amygdalin ChEBI	+	fermentation	* from API 20E
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE, API 20E
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	fermentation	* from API 20NE, API 20E
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68368]	D-mannitol ChEBI	+	fermentation	* from API 20E
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE, API 20E
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68368]	L-arabinose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	lysine ChEBI	+	degradation	* from API 20E
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68368]	melibiose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	myo-inositol ChEBI	+	fermentation	* from API 20E
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #68368]	sorbitol ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	sucrose ChEBI	+	fermentation	* from API 20E
[Ref.: #68369]	tryptophan ChEBI	+	energy source	* from API 20NE, API 20E
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE, API 20E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68369]	indole ChEBI	yes	* from API 20NE, API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68369]		indole ChEBI		+	* from API 20NE, API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE, API 20E
[Ref.: #68368]	beta-galactosidase	+	3.2.1.23	* from API 20E
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE
[Ref.: #68369]	cytochrome oxidase	-	1.9.3.1	* from API 20NE, API 20E
[Ref.: #68369]	gelatinase	-		* from API 20NE, API 20E
[Ref.: #68368]	lysine decarboxylase	+	4.1.1.18	* from API 20E
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE, API 20E

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #19209]	17975	+	-	+	-	+	-	-	-	+	+	-	+	+	+	+	+	+	+	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #19209]	24892	+	+	+	-	-	+	-	+	+	+	+	+	+	+	+	-	-	+	+	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #19209]	Sample type/isolated from	tooth brush holder
[Ref.: #19209]	Geographic location (country and/or sea, region)	Ann Arbor, MI
[Ref.: #19209]	Country	USA
[Ref.: #19209]	Country ISO 3 Code	USA
[Ref.: #19209]	Continent	North America

[Ref.: #63343]	Sample type/isolated from	Tooth brush holder
[Ref.: #63343]	Geographic location (country and/or sea, region)	Ann Arbor,MI
[Ref.: #63343]	Country	USA
[Ref.: #63343]	Country ISO 3 Code	USA
[Ref.: #63343]	Continent	North America

[Ref.: #120943]	Sample type/isolated from	Tooth brush holder
[Ref.: #120943]	Geographic location (country and/or sea, region)	Ann Arbor, Michigan
[Ref.: #120943]	Country	United States of America
[Ref.: #120943]	Country ISO 3 Code	USA
[Ref.: #120943]	Continent	North America
* marker position based on {}

Isolation sources categories

#Engineered

#Industrial

#Engineered product

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JQ070300 (>99% sequence identity) for Enterobacteriaceae from Microbeatlas

Aquatic Samples	233
Soil Samples	121
Animal Samples	3440
Plant Samples	216
Total Samples	4010

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

Biosafety level

Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #19209]	Klebsiella michiganensis strain W14 16S ribosomal RNA gene, partial sequence	JQ070300	1395	ENA	1134687 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Klebsiella michiganensis CCUG 66515	GCA_009173485	contig	GenBank	1134687 tax ID	*
[Ref.: #66792]	Klebsiella michiganensis strain CCUG 66515	1134687.164	wgs	PATRIC	1134687 tax ID	*
[Ref.: #66792]	Klebsiella michiganensis strain DSM 25444	1134687.61	wgs	PATRIC	1134687 tax ID	*
[Ref.: #66792]	Klebsiella michiganensis DSM 25444	2916627578	draft	JGI IMG/M	1134687 tax ID	*
[Ref.: #66792]	Klebsiella michiganensis DSM 25444	GCA_002925905	scaffold	GenBank	1134687 tax ID	*

GC-content

54.6 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Klebsiella michiganensis DSM 25444 NCBI: GCA_002925905.1
[Ref.: #69480]	flagellated	no	92.502	no
[Ref.: #69480]	gram-positive	no	97.569	no
[Ref.: #69480]	anaerobic	no	96.582	yes
[Ref.: #69480]	aerobic	yes	85.7	yes
[Ref.: #69480]	halophile	no	90.963	no
[Ref.: #69480]	spore-forming	no	94.323	no
[Ref.: #69480]	glucose-util	yes	93.753	yes
[Ref.: #69480]	motile	no	81.43	no
[Ref.: #69480]	glucose-ferment	yes	88.573	yes
[Ref.: #69480]	thermophile	no	99.694	no

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