Name: Tonsilliphilus suis W254
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 22988

Type strain:

Species: Tonsilliphilus suis

Strain Designation: W254

Culture col. no.: DSM 21880, ATCC 35846, CCM 3774, JCM 15727

Strain history: S. Murakami W254.

NCBI tax ID(s): 214014 (species)

SI-ID 66937

CCM 3774, ATCC 35846, DSM 21880, JCM 15727

Section

Tonsilliphilus suis W254 is an anaerobe, spore-forming, mesophilic animal pathogen that was isolated from submandibular lymph node of an infected pig.

coccus-shaped
Gram-positive
motile
spore-forming
anaerobe
animal pathogen
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Dermatophilaceae*
	Genus	*Tonsilliphilus*
	Species	**Tonsilliphilus suis**
	Full Scientific Name (LPSN)	Tonsilliphilus suis Azuma et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #30821]	Gram stain	positive

[Ref.: #30821]	Cell shape	coccus-shaped

[Ref.: #30821]	Motility	yes

[Ref.: #19363]

Incubation period

3-7 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #19363]

Culture medium

PYG MEDIUM (MODIFIED) (DSMZ Medium 104)

[Ref.: #19363]

Culture medium growth

[Ref.: #19363]

Culture medium link

Medium recipe at MediaDive

[Ref.: #19363]

Culture medium composition

expand / minimize

[Ref.: #19363]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #19363]

Culture medium growth

[Ref.: #19363]

Culture medium link

Medium recipe at MediaDive

[Ref.: #19363]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #19363]	growth	37 °C
[Ref.: #30821]	optimum	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #19363]	Temperature range	mesophilic
[Ref.: #30821]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #19363]	Oxygen tolerance	anaerobe
[Ref.: #30821]	Oxygen tolerance	anaerobe

[Ref.: #30821]

Ability of spore formation

yes

[Ref.: #67770]

Observation

quinones: MK-8(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #30821]	esculin ChEBI	+	hydrolysis
[Ref.: #30821]	fructose ChEBI	+	carbon source
[Ref.: #30821]	galactose ChEBI	+	carbon source
[Ref.: #30821]	glucose ChEBI	+	carbon source
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #30821]	lactose ChEBI	+	carbon source
[Ref.: #30821]	maltose ChEBI	+	carbon source
[Ref.: #30821]	mannitol ChEBI	+	carbon source
[Ref.: #30821]	mannose ChEBI	+	carbon source
[Ref.: #30821]	melibiose ChEBI	+	carbon source
[Ref.: #30821]	nitrate ChEBI	+	reduction
[Ref.: #68380]	nitrate ChEBI	+	reduction	* from API rID32A
[Ref.: #30821]	raffinose ChEBI	+	carbon source
[Ref.: #68380]	raffinose ChEBI	+	fermentation	* from API rID32A
[Ref.: #30821]	salicin ChEBI	+	carbon source
[Ref.: #30821]	sorbitol ChEBI	+	carbon source
[Ref.: #30821]	sucrose ChEBI	+	carbon source
[Ref.: #30821]	trehalose ChEBI	+	carbon source
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68380]	alanine arylamidase	-	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	-	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #68380]	alpha-galactosidase	+	3.2.1.22	* from API rID32A
[Ref.: #68380]	alpha-glucosidase	+	3.2.1.20	* from API rID32A
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-galactosidase	+	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	+	3.2.1.21	* from API rID32A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	+		* from API rID32A
[Ref.: #68380]	leucine arylamidase	+	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32A
[Ref.: #68380]	phenylalanine arylamidase	+		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	+		* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #19363]	12751	-	-	+	+	-	+	+	-	-	-	+/-	+	-	-	+	-	-	+	-	-	+	+	-	+	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #19363]	Sample type/isolated from	submandibular lymph node of an infected pig
[Ref.: #19363]	Country	Japan
[Ref.: #19363]	Country ISO 3 Code	JPN
[Ref.: #19363]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Infected swine submandibular lymph node
* marker position based on {}

Isolation sources categories

#Infection	#Disease	-
#Host	#Mammals	#Suidae (Pig,Swine)
#Host Body-Site	#Oral cavity and airways	-
#Host Body-Site	#Organ	#Lymph node

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #19363]	Pathogenicity (animal)	yes
[Ref.: #19363]	Biosafety level	2	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #19363]	Tonsilliphilus suis gene for 16S rRNA, partial sequence, strain: W254	AB096084	1534	ENA	214014 tax ID

[Ref.: #19363]	GC-content	72.2 mol%	high performance liquid chromatography (HPLC)
[Ref.: #30821]	GC-content	72.2 mol%

Availability in culture collections External links

[Ref.: #19363]

Culture collection no.

DSM 21880, ATCC 35846, CCM 3774, JCM 15727

[Ref.: #87234]

SI-ID 66937

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Tonsilliphilus suis gen. nov., sp. nov., causing tonsil infections in pigs.	Azuma R, Ung-Bok B, Murakami S, Ishiwata H, Osaki M, Shimada N, Ito Y, Miyagawa E, Makino T, Kudo T, Takahashi Y, Yano I, Murata R, Yokoyama E	Int J Syst Evol Microbiol	10.1099/ijs.0.045237-0	2012	*

References

#19363

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21880

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30821

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27152 (see below)

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68380

Automatically annotated from API rID32A .

#87234

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID66937.1 )

#27152

IJSEM 2545 2013 ( DOI 10.1099/ijs.0.045237-0 , PubMed 23243095 )

* These data were automatically processed and therefore are not curated

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