Name: Castellaniella hirudinis E103
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 22904

Type strain:

Species: Castellaniella hirudinis

Strain Designation: E103

Culture col. no.: DSM 25756, CCUG 62394, LMG 26910, CIP 110374

Strain history: CIP <- 2012, P. Kämpfer, Giessen Univ., Giessen, Germany: strain E103 <- 2009, L. Kaiser

NCBI tax ID(s): 1144617 (species)

SI-ID 400990

DSM 25756, CCUG 62394

Section

Castellaniella hirudinis E103 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from skin of a juvenile medical leech .

Gram-negative
motile
rod-shaped
anaerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Alcaligenaceae*
	Genus	*Castellaniella*
	Species	**Castellaniella hirudinis**
	Full Scientific Name (LPSN)	Castellaniella hirudinis Glaeser et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #30662]	Gram stain	negative
[Ref.: #119913]	Gram stain	negative

[Ref.: #30662]	Cell length	1 µm

[Ref.: #30662]	Cell width	0.5 µm

[Ref.: #30662]	Cell shape	rod-shaped
[Ref.: #119913]	Cell shape	rod-shaped

[Ref.: #30662]	Motility	yes
[Ref.: #119913]	Motility	yes

[Ref.: #30662]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #32803]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #32803]

Culture medium growth

[Ref.: #32803]

Culture medium composition

expand / minimize

[Ref.: #19219]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #19219]

Culture medium growth

[Ref.: #19219]

Culture medium link

Medium recipe at MediaDive

[Ref.: #19219]

Culture medium composition

expand / minimize

[Ref.: #119913]

Culture medium

CIP Medium 566

[Ref.: #119913]

Culture medium growth

[Ref.: #119913]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #119913]

Culture medium

CIP Medium 72

[Ref.: #119913]

Culture medium growth

[Ref.: #119913]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #19219]	growth	28 °C
[Ref.: #30662]	growth	15-37 °C
[Ref.: #30662]	optimum	27.5 °C
[Ref.: #32803]	growth	25 °C
[Ref.: #62825]	growth	30 °C

[Ref.: #19219]	Temperature range	mesophilic
[Ref.: #30662]	Temperature range	mesophilic
[Ref.: #32803]	Temperature range	mesophilic
[Ref.: #62825]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #30662]			optimum	8.25
[Ref.: #30662]			growth	5.5-11

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30662]	Oxygen tolerance	anaerobe
[Ref.: #62825]	Oxygen tolerance	aerobe
[Ref.: #119913]	Oxygen tolerance	obligate aerobe

[Ref.: #30662]

Ability of spore formation

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30662]	3-hydroxybutyrate ChEBI	+	carbon source
[Ref.: #30662]	acetate ChEBI	+	carbon source
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #30662]	alanine ChEBI	+	carbon source
[Ref.: #30662]	arabinose ChEBI	+	carbon source
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #30662]	aspartate ChEBI	+	carbon source
[Ref.: #30662]	cellobiose ChEBI	+	carbon source
[Ref.: #30662]	citrate ChEBI	+	carbon source
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #30662]	fructose ChEBI	+	carbon source
[Ref.: #30662]	galactose ChEBI	+	carbon source
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #30662]	gluconate ChEBI	+	carbon source
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #30662]	glucose ChEBI	+	carbon source
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #30662]	lactate ChEBI	+	carbon source
[Ref.: #30662]	leucine ChEBI	+	carbon source
[Ref.: #30662]	malate ChEBI	+	carbon source
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #30662]	maltose ChEBI	+	carbon source
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #30662]	mannose ChEBI	+	carbon source
[Ref.: #30662]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #119913]	nitrate ChEBI	+	reduction
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #119913]	nitrite ChEBI	+	reduction
[Ref.: #30662]	ornithine ChEBI	+	carbon source
[Ref.: #30662]	proline ChEBI	+	carbon source
[Ref.: #30662]	propionate ChEBI	+	carbon source
[Ref.: #30662]	pyruvate ChEBI	+	carbon source
[Ref.: #30662]	serine ChEBI	+	carbon source
[Ref.: #30662]	sucrose ChEBI	+	carbon source
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #119913]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE, API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #30662]	catalase	+	1.11.1.6
[Ref.: #119913]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #30662]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119913]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #119913]	urease	-	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #19219]	24497	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	+	+	+	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119913]	12558	-	-	+	-	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #19219]	Sample type/isolated from	skin of a juvenile medical leech (Hirudo verbana)
[Ref.: #19219]	Host species	Hirudo verbana
[Ref.: #19219]	Geographic location (country and/or sea, region)	Hessen, Biebertal
[Ref.: #19219]	Country	Germany
[Ref.: #19219]	Country ISO 3 Code	DEU
[Ref.: #19219]	Continent	Europe

[Ref.: #62825]	Sample type/isolated from	Surface,juvenile leech
[Ref.: #62825]	Geographic location (country and/or sea, region)	Biebertal (Hessen)
[Ref.: #62825]	Country	Germany
[Ref.: #62825]	Country ISO 3 Code	DEU
[Ref.: #62825]	Continent	Europe

[Ref.: #119913]	Sample type/isolated from	Animal, From the surface of a juvenile leech
[Ref.: #119913]	Geographic location (country and/or sea, region)	Biebertal, Hessen
[Ref.: #119913]	Country	Germany
[Ref.: #119913]	Country ISO 3 Code	DEU
[Ref.: #119913]	Continent	Europe
[Ref.: #119913]	Isolation date	2009
* marker position based on {}

Isolation sources categories

#Host	#Invertebrates (Other)	#Annelida
#Host Body-Site	#Organ	#Skin, Nail, Hair

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JQ319891 (>99% sequence identity) for Castellaniella from Microbeatlas

Aquatic Samples	938
Soil Samples	445
Animal Samples	391
Plant Samples	131
Total Samples	1905

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #19219]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119913]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #19219]	Castellaniella hirudinis strain E103 16S ribosomal RNA gene, partial sequence	JQ319891	1368	ENA	1144617 tax ID

Availability in culture collections External links

[Ref.: #19219]

Culture collection no.

DSM 25756, CCUG 62394, LMG 26910, CIP 110374

[Ref.: #87152]

SI-ID 400990

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Castellaniella hirudinis sp. nov., isolated from the skin of Hirudo verbana.	Glaeser SP, Galatis H, Martin K, Kampfer P	Int J Syst Evol Microbiol	10.1099/ijs.0.041392-0	2012	*

References

#19219

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25756

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30662

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26993 (see below)

#32803 ; Curators of the CIP;
#62825 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 62394

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#87152

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID400990.1 )

#119913 Collection of Institut Pasteur ; Curators of the CIP; CIP 110374

#26993

IJSEM 521 2013 ( DOI 10.1099/ijs.0.041392-0 , PubMed 22523161 )

* These data were automatically processed and therefore are not curated

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