Granulicella mallensis (DSM 23137, ATCC BAA-1857, MP5ACTX8)

Name: Granulicella mallensis (DSM 23137, ATCC BAA-1857, MP5ACTX8)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 22899

Type strain

Strain Designation MP5ACTX8

Culture col. no. DSM 23137 ATCC BAA-1857 MP5ACTX8

NCBI tax ID(s) 940614

Special Collections DSMZ Literature strains

Links

Granulicella mallensis MP5ACTX8 is an aerobe, psychrophilic, Gram-negative prokaryote that was isolated from tundra soil.

Gram-negative rod-shaped aerobe psychrophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 397245

ATCC BAA-1857,DSM 23137

@ref 20215

Last LPSN update 2025-12-16 09:52:31

Domain Pseudomonadati

Phylum Acidobacteriota

Class Terriglobia

Order Terriglobales

Family Acidobacteriaceae

Genus Granulicella

Species Granulicella mallensis

Full scientific name Granulicella mallensis Männistö et al. 2012

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
30254	negative	1 µm	0.6 µm	rod-shaped
26596	negative	0.6-1.3 µm	0.5-0.7 µm	rod-shaped
125438	negative				92.205

Colony morphology

@ref	Colony color	Colony shape	Medium used	Incubation period
19168				8-14 days
26596	white to light beige	circular	R2A-Agar

Pigmentation

30254

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
26596	R2A-Agar
19168	R2A MEDIUM (DSMZ Medium 830)	Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
19168	SSE/HD1:10 (DSMZ Medium 1426)	Medium recipe at MediaDive	Name: SSE/HD 1:10 MEDIUM (DSMZ Medium 1426) Composition: MES 1.95 g/l Peptone 0.5 g/l CaSO4 x 2 H2O 0.4303 g/l MgSO4 x 7 H2O 0.3695 g/l Yeast extract 0.25 g/l Na NO3 0.212 g/l CaCl2 x 2 H2O 0.1469 g/l Ca(NO3)2 x 4 H2O 0.118 g/l MgCl2 x 6 H2O 0.1018 g/l Glucose 0.1 g/l (NH4)2SO4 0.09915 g/l NH4Cl 0.05345 g/l K2SO4 0.0435 g/l FeSO4 x 7 H2O 0.00555 g/l KH2PO4 0.00340213 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Pyridoxine hydrochloride 1e-05 g/l H3BO3 6e-06 g/l Lipoic acid 5e-06 g/l D-Calcium pantothenate 5e-06 g/l Nicotinic acid 5e-06 g/l Riboflavin 5e-06 g/l Thiamine-HCl x 2 H2O 5e-06 g/l p-Aminobenzoic acid 5e-06 g/l Folic acid 2e-06 g/l Biotin 2e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Vitamin B12 1e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
19168	positive	growth	20	psychrophilic
26596	positive	optimum	24-27
26596	positive	growth	4-28
30254	positive	growth	4-28
30254	positive	optimum	25.5	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
30254	positive	optimum	5
26596	positive	optimum	5
30254	positive	growth	3.5-6.5	acidophile
26596	positive	growth	3.5-6.5

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
30254	aerobe
26596	aerobe
125439	obligate aerobe	99.2

Spore formation

26596

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
30254	NaCl	positive	growth	<1.5 %
26596	NaCl	positive	growth	0-1.5 %(w/v)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
26596	30089 ChEBI	acetate	-	carbon source
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
26596	35391 ChEBI	aspartate	-	carbon source
26596	17968 ChEBI	butyrate	-	carbon source
30254	17057 ChEBI	cellobiose	+	carbon source
26596	17057 ChEBI	cellobiose	+	carbon source
26596	17029 ChEBI	chitin	-	hydrolysis
26596	16261 ChEBI	chitosan	-	hydrolysis
26596	15570 ChEBI	D-alanine	-	carbon source
26596	17108 ChEBI	D-arabinose	-	carbon source
26596	15824 ChEBI	D-fructose	+	carbon source
26596	12936 ChEBI	D-galactose	+	carbon source
26596	8391 ChEBI	D-gluconate	-	carbon source
26596	17634 ChEBI	D-glucose	+	carbon source
68369	17634 ChEBI	D-glucose	-	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
26596	62318 ChEBI	D-lyxose	-	carbon source
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
26596	16024 ChEBI	D-mannose	+	carbon source
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
26596	16988 ChEBI	D-ribose	+	carbon source
26596	17924 ChEBI	D-sorbitol	-	carbon source
26596	65327 ChEBI	D-xylose	+	carbon source
26596	27689 ChEBI	decanoate	-	carbon source
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
30254	4853 ChEBI	esculin	+	hydrolysis
26596	4853 ChEBI	esculin	+	hydrolysis
68369	4853 ChEBI	esculin	+	hydrolysis	from API 20NE
26596	16236 ChEBI	ethanol	-	carbon source
26596	15740 ChEBI	formate	-	carbon source
30254	28757 ChEBI	fructose	+	carbon source
26596	29806 ChEBI	fumarate	-	carbon source
26596	16813 ChEBI	galactitol	-	carbon source
30254	28260 ChEBI	galactose	+	carbon source
26596	5291 ChEBI	gelatin	-	hydrolysis
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	-	assimilation	from API 20NE
30254	17234 ChEBI	glucose	+	carbon source
26596	24297 ChEBI	glucuronate	+	carbon source
30254	29987 ChEBI	glutamate	+	carbon source
26596	29987 ChEBI	glutamate	+	carbon source
26596	15428 ChEBI	glycine	-	carbon source
26596	85249 ChEBI	hydroxyethylcellulose	-	hydrolysis
26596		L-alanine 4-nitroanilide	-	carbon source
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
26596	15603 ChEBI	L-leucine	-	carbon source
26596	15729 ChEBI	L-ornithine	-	carbon source
26596	24996 ChEBI	lactate	-	carbon source
30254	17716 ChEBI	lactose	+	carbon source
26596	17716 ChEBI	lactose	+	carbon source
26596	6359 ChEBI	lactulose	+	carbon source
26596	6364 ChEBI	laminarin	+	hydrolysis
26596	6452 ChEBI	lichenin	+	hydrolysis
26596	25115 ChEBI	malate	-	carbon source
68369	25115 ChEBI	malate	-	assimilation	from API 20NE
30254	17306 ChEBI	maltose	+	carbon source
26596	17306 ChEBI	maltose	+	carbon source
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
26596	29864 ChEBI	mannitol	-	carbon source
30254	37684 ChEBI	mannose	+	carbon source
26596	6731 ChEBI	melezitose	+	carbon source
26596	17790 ChEBI	methanol	-	carbon source
26596	17268 ChEBI	myo-inositol	-	carbon source
30254	506227 ChEBI	N-acetylglucosamine	+	carbon source
26596	506227 ChEBI	N-acetylglucosamine	+	carbon source
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
26596	17632 ChEBI	nitrate	+	reduction
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
26596	30623 ChEBI	oxalate	-	carbon source
26596	17309 ChEBI	pectin	+	hydrolysis
26596	17272 ChEBI	propionate	-	carbon source
26596	27941 ChEBI	pullulan	-	hydrolysis
26596	15361 ChEBI	pyruvate	-	carbon source
30254	16634 ChEBI	raffinose	+	carbon source
26596	16634 ChEBI	raffinose	+	carbon source
26596	15963 ChEBI	ribitol	-	carbon source
30254	33942 ChEBI	ribose	+	carbon source
30254	17814 ChEBI	salicin	+	carbon source
26596	17814 ChEBI	salicin	+	carbon source
26596	53311 ChEBI	sodium alginate	-	hydrolysis
26596	28017 ChEBI	starch	+	hydrolysis
26596	30031 ChEBI	succinate	-	carbon source
30254	17992 ChEBI	sucrose	+	carbon source
26596	17992 ChEBI	sucrose	+	carbon source
30254	27082 ChEBI	trehalose	+	carbon source
26596	27082 ChEBI	trehalose	+	carbon source
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE
26596	31011 ChEBI	valerate	-	carbon source
26596	37166 ChEBI	xylan	-	hydrolysis
30254	18222 ChEBI	xylose	+	carbon source

Antibiotic resistance

@ref	ChEBI	Metabolite	Sensitivity conc.	Resistance conc.
26596	28971	ampicillin	10 µg (disc)
26596	28669	bacitracin	10 Unit
26596	17698	chloramphenicol		30 µg (disc)
26596	48923	erythromycin		15 µg (disc)
26596	17833	gentamicin		10 µg (disc)
26596	6104	kanamycin	30 µg (disc)
26596	6472	lincomycin	19 µg (disc)
26596	7507	neomycin		10 µg (disc)
26596	28368	novobiocin	10 µg (disc)
26596	17334	penicillin		10 Unit
26596	8309	polymyxin b		300 Unit
26596	28077	rifampicin		10 µg (disc)
26596	17076	streptomycin		10 µg (disc)
26596	27902	tetracycline	5 µg (disc)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
30254	acid phosphatase	+	3.1.3.2
26596	acid phosphatase	+	3.1.3.2
68382	acid phosphatase	+	3.1.3.2	from API zym
30254	alkaline phosphatase	+	3.1.3.1
26596	alkaline phosphatase	+	3.1.3.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
26596	alpha-chymotrypsin	+	3.4.21.1
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
26596	alpha-fucosidase	-	3.2.1.51
68382	alpha-fucosidase	-	3.2.1.51	from API zym
30254	alpha-galactosidase	+	3.2.1.22
26596	alpha-galactosidase	+	3.2.1.22
68382	alpha-galactosidase	-	3.2.1.22	from API zym
26596	alpha-glucosidase	+	3.2.1.20
68382	alpha-glucosidase	-	3.2.1.20	from API zym
26596	alpha-mannosidase	-	3.2.1.24
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
26596	beta-galactosidase	+	3.2.1.23
26596	beta-glucosidase	+	3.2.1.21
68369	beta-glucosidase	+	3.2.1.21	from API 20NE
26596	beta-glucuronidase	+	3.2.1.31
30254	catalase	+	1.11.1.6
26596	catalase	+	1.11.1.6
26596	cystine arylamidase	-	3.4.11.3
68382	cystine arylamidase	-	3.4.11.3	from API zym
26596	cytochrome oxidase	+	1.9.3.1
26596	esterase (C 4)	-
68382	esterase (C 4)	-		from API zym
26596	esterase Lipase (C 8)	-
68382	esterase lipase (C 8)	-		from API zym
68369	gelatinase	-		from API 20NE
26596	leucine arylamidase	+	3.4.11.1
68382	leucine arylamidase	+	3.4.11.1	from API zym
26596	lipase (C 14)	-
68382	lipase (C 14)	-		from API zym
26596	N-acetyl-beta-glucosaminidase	+	3.2.1.52
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
26596	naphthol-AS-BI-phosphohydrolase	+
26596	trypsin	+	3.4.21.4
68382	trypsin	-	3.4.21.4	from API zym
68369	urease	-	3.5.1.5	from API 20NE
26596	valine arylamidase	+
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
19168	-	+	-	-	-	+	+	-	-	-	+	+/-	-	+/-	+/-	-	+/-	+	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
19168	-	-	-	-	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil
#Climate	#Cold	#Tundra
#Environmental	#Terrestrial	#Tundra

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude
19168	tundra soil	Lapland, Kilpisjärvi	Finland	FIN	Europe
26596	tundra soil	Finland	Finland	FIN	Europe	69	20 69/20

Taxonmaps

69479

Global distribution of 16S sequence HQ687087 (>99% sequence identity) for Granulicella mallensis subclade from Microbeatlas

Aquatic Samples	49
Soil Samples	145
Animal Samples	57
Plant Samples	12
Total Samples	263

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
19168	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM17895v2 assembly for Granulicella mallensis MP5ACTX8	complete	GCA_000178955	682795.3	2503982037	682795	97.06

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
19168	Granulicella mallensis strain MP5ACTX8 16S ribosomal RNA gene, partial sequence	HQ687087	1488		682795

GC content

@ref	GC-content (mol%)	Method
19168	57.8	high performance liquid chromatography (HPLC)
30254	57.8

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Granulicella mallensis MP5ACTX8
IMG: 2503982037

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	72.40	no
125439	motility	BacteriaNetⓘ	no	56.60	no
125439	gram_stain	BacteriaNetⓘ	negative	88.20	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.20	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Granulicella mallensis MP5ACTX8 MP5ACTX8
NCBI: GCA_000178955.2

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	92.21	yes
125438	anaerobic	anaerobicⓘ	no	86.81	yes
125438	aerobic	aerobicⓘ	yes	85.58	yes
125438	spore-forming	spore-formingⓘ	no	82.03	no
125438	thermophilic	thermophileⓘ	no	93.32	yes
125438	flagellated	motile2+ⓘ	no	76.82	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Cloning, heterologous expression, and characterization of novel protease-resistant alpha-galactosidase from new Sphingomonas strain.	Zhou J, Dong Y, Li J, Zhang R, Tang X, Mu Y, Xu B, Wu Q, Huang Z	J Microbiol Biotechnol	10.4014/jmb.1112.12036	2012
Metabolism	Comparative genomic and physiological analysis provides insights into the role of Acidobacteria in organic carbon utilization in Arctic tundra soils.	Rawat SR, Mannisto MK, Bromberg Y, Haggblom MM	FEMS Microbiol Ecol	10.1111/j.1574-6941.2012.01381.x	2012
Enzymology	Structural basis for double cofactor specificity in a new formate dehydrogenase from the acidobacterium Granulicella mallensis MP5ACTX8.	Fogal S, Beneventi E, Cendron L, Bergantino E	Appl Microbiol Biotechnol	10.1007/s00253-015-6695-x	2015
Genetics	Complete genome sequence of Granulicella mallensis type strain MP5ACTX8(T), an acidobacterium from tundra soil.	Rawat SR, Mannisto MK, Starovoytov V, Goodwin L, Nolan M, Hauser LJ, Land M, Davenport KW, Woyke T, Haggblom MM	Stand Genomic Sci	10.4056/sigs.4328031	2013
Phylogeny	Granulicella arctica sp. nov., Granulicella mallensis sp. nov., Granulicella tundricola sp. nov. and Granulicella sapmiensis sp. nov., novel acidobacteria from tundra soil.	Mannisto MK, Rawat S, Starovoytov V, Haggblom MM	Int J Syst Evol Microbiol	10.1099/ijs.0.031864-0	2011

References

#19168	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23137
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#26596	IJSEM 2097 2012 ( DOI 10.1099/ijs.0.031864-0 , PubMed 22058325 )
#30254	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26596 (see below)
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Granulicella mallensis (DSM 23137, ATCC BAA-1857, MP5ACTX8)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API zym

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000178955

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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