Komagataeibacter intermedius (DSM 13111, ZIM B022, JK3)

Name: Komagataeibacter intermedius (DSM 13111, ZIM B022, JK3)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 22896

Type strain

Strain Designation JK3

Culture col. no. DSM 13111 ZIM B022 JK3 CIP 105781

NCBI tax ID(s) 66229

Special Collections DSMZ CIP

History DSMZ <- J. Trcek, Univ. Ljubljana, Slovenia; JK3 CIP <- 1998, C. Boesch, Zürich, Switzerland: strain JK3 <- ZIM

Links

Komagataeibacter intermedius JK3 is an obligate aerobe, Gram-negative, rod-shaped bacterium that was isolated from cider vinegar.

Gram-negative rod-shaped obligate aerobe 16S sequence Bacteria

Name and taxonomic classification

SI-ID 67007

CIP 105781

@ref 20215

Last LPSN update 2026-05-29 08:30:20

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Rhodospirillales

Family Acetobacteraceae

Genus Komagataeibacter

Species Komagataeibacter intermedius

Full scientific name Komagataeibacter intermedius (Boesch et al. 1998) Yamada et al. 2013

Synonyms (3)

Acetobacter intermedius
"Komagatabacter intermedius"
Gluconacetobacter intermedius

BacDive ID	Other strains from **Komagataeibacter intermedius** (2)	Type strain
92	K. intermedius TF2, DSM 11804, BCC 36457, JCM 16936, LMG ... (type strain)
138240	K. intermedius TSN3, CIP 105782, ZIM B051

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
120152	negative	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
19146	GLUCONACETOBACTER MEDIUM (DSMZ Medium 1430)	Medium recipe at MediaDive	Name: GLUCONACETOBACTER MEDIUM (DSMZ Medium 1430) Composition: Agar 15.0 g/l Glucose 5.0 g/l Peptone 4.0 g/l Yeast extract 3.0 g/l Acetic acid Ethanol Distilled water
40401	MEDIUM 289 - for Acetobacter intermedius		Distilled water make up to (940.000 ml);Agar (8.000 g);Glucose (7.500 g);Yeast extract (3.000 g);Peptone (4.000 g);Acetic acid (30.000 ml);Ethanol (30.000 ml)
120152	CIP Medium 289	Medium recipe at CIP
19146	MRS MEDIUM (DSMZ Medium 11)	Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
19146	positive	growth	30
40401	positive	growth	30
120152	positive	growth	22-30
120152	negative	growth	10
120152	negative	growth	37
120152	negative	growth	41
120152	negative	growth	45

Physiology and metabolism

Oxygen tolerance

120152

Oxygen toleranceobligate aerobe

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
120152	606565 ChEBI	hippurate	-	hydrolysis
120152	17632 ChEBI	nitrate	-	reduction
120152	16301 ChEBI	nitrite	+	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
120152	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
120152	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
120152	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
120152	gamma-glutamyltransferase	-	2.3.2.2
120152	gelatinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
120152	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
120152	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
120152	-	-	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Engineered	#Food production
#Engineered	#Industrial

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
19146	cider vinegar		Slovenia	SVN	Europe
120152	Cider vinegar production acetator	Ljubljana	Slovenia	SVN	Europe	1995

Taxonmaps

69479

Global distribution of 16S sequence AJ012699 (>99% sequence identity) for Komagataeibacter from Microbeatlas

Aquatic Samples	523
Soil Samples	662
Animal Samples	2327
Plant Samples	555
Total Samples	4067

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
19146	1	Risk group (German classification) According to TRBA 466
120152	1	Risk group (French classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
19146	Acetobacter intermedius, 16S rRNA gene, ZIM B022	AJ012699	1481		66229

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	16S rRNA in situ Hybridization Followed by Flow Cytometry for Rapid Identification of Acetic Acid Bacteria Involved in Submerged Industrial Vinegar Production.	Trcek J, Lipoglavsek L, Avgustin G.	Food Technol Biotechnol	10.17113/ftb.54.01.16.4041	2016

References

#19146	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13111
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#40401	; Curators of the CIP;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120152	Collection of Institut Pasteur ; Curators of the CIP; CIP 105781
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Komagataeibacter intermedius (DSM 13111, ZIM B022, JK3)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Metabolite utilization

Metabolite production

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

Genome-based predictions

Literature

Literature

References

BacDive

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