Name: Carnobacterium alterfunditum pf4
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2266

Type strain:

Species: Carnobacterium alterfunditum

Strain Designation: pf4

Culture col. no.: DSM 5972, ACAM 313, ATCC 49837, CCUG 34643, IFO 15548, CIP 105796

Strain history: CIP <- 1998, ATCC <- N. Weiss <- P. Franzmann: strain Pf4

NCBI tax ID(s): 1449335 (strain), 28230 (species)

SI-ID 8234

LMG 14462, ATCC 49837, CCUG 34643, DSM 5972, IFO 15548, LMG 13520, NCFB 3003, NCIMB 13248, CIP 105796, DSM 5973, CECT 5892, NBRC 15548, JCM 12498

Other strains from Carnobacterium alterfunditum

C. alterfunditum pf1, DSM 5973

Section

Carnobacterium alterfunditum pf4 is an aerobe, psychrophilic, Gram-positive bacterium that was isolated from water.

Gram-positive
aerobe
psychrophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Carnobacteriaceae*
	Genus	*Carnobacterium*
	Species	**Carnobacterium alterfunditum**
	Full Scientific Name (LPSN)	Carnobacterium alterfunditum Franzmann et al. 1993

Information on morphological and physiological properties Morphology

[Ref.: #116268]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #116268]	Cell shape	rod-shaped

[Ref.: #116268]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #2399]

Culture medium

TSBY SALT MEDIUM (DSMZ Medium 466)

[Ref.: #2399]

Culture medium growth

[Ref.: #2399]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2399]

Culture medium composition

expand / minimize

[Ref.: #40197]

Culture medium

MEDIUM 297 - for Carnobacterium alberfunditum

[Ref.: #40197]

Culture medium growth

[Ref.: #40197]

Culture medium composition

expand / minimize

[Ref.: #116268]

Culture medium

CIP Medium 297

[Ref.: #116268]

Culture medium growth

[Ref.: #116268]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #2399]		growth	20 °C
[Ref.: #40197]		growth	20 °C
[Ref.: #52228]		growth	16-22 °C
[Ref.: #116268]		growth	5-30 °C
[Ref.: #116268]	no	growth	37 °C
[Ref.: #116268]	no	growth	41 °C

[Ref.: #2399]	Temperature range	psychrophilic
[Ref.: #40197]	Temperature range	psychrophilic
[Ref.: #52228]	Temperature range	psychrophilic
[Ref.: #116268]	Temperature range	mesophilic
[Ref.: #116268]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #52228]	Oxygen tolerance	aerobe
[Ref.: #116268]	Oxygen tolerance	facultative anaerobe

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.97

	Salt	Tested relation		Salt conc.
[Ref.: #116268]	NaCl	no	growth	0	%
[Ref.: #116268]	NaCl	no	growth	2	%
[Ref.: #116268]	NaCl	no	growth	4	%
[Ref.: #116268]	NaCl	no	growth	6	%
[Ref.: #116268]	NaCl	no	growth	8	%
[Ref.: #116268]	NaCl	no	growth	10	%

[Ref.: #2399]	Murein short key	A31
[Ref.: #2399]	Murein types	A1gamma m-Dpm-direct

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	arbutin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #116268]	citrate ChEBI	-	carbon source
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #116268]	esculin ChEBI	+	hydrolysis
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #116268]	hippurate ChEBI	+	hydrolysis
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #116268]	nitrate ChEBI	-	builds gas from
[Ref.: #116268]	nitrate ChEBI	-	reduction
[Ref.: #116268]	nitrite ChEBI	-	builds gas from
[Ref.: #116268]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #116268]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #116268]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #116268]	amylase	-
[Ref.: #116268]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #116268]	caseinase	-	3.4.21.50
[Ref.: #116268]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #116268]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #116268]	gelatinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #116268]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #116268]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #116268]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #116268]	tryptophan deaminase	-
[Ref.: #116268]	tween esterase	-
[Ref.: #116268]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #116268]	10236	not determinedn.d.	-	-	-	-	+/-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	+	+/-	+	+	+	+/-	+	-	-	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116268]	9001	-	-	-	-	-	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #2399]	Sample type/isolated from	water
[Ref.: #2399]	Geographic location (country and/or sea, region)	Vestfold Hills, Ace Lake, depth 24 m
[Ref.: #2399]	Continent	Australia and Oceania

[Ref.: #52228]	Sample type/isolated from	Water
[Ref.: #52228]	Geographic location (country and/or sea, region)	Vestfold Hills,Ace Lake
[Ref.: #52228]	Country	Antarctica
[Ref.: #52228]	Country ISO 3 Code	ATA
[Ref.: #52228]	Continent	Australia and Oceania

[Ref.: #116268]	Sample type/isolated from	Environment, Ace lake
[Ref.: #116268]	Geographic location (country and/or sea, region)	Vestfold hills
[Ref.: #116268]	Country	Antarctica
[Ref.: #116268]	Country ISO 3 Code	ATA
[Ref.: #116268]	Continent	Antarctica
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB680898 (>99% sequence identity) for Carnobacterium from Microbeatlas

Aquatic Samples	370
Soil Samples	303
Animal Samples	580
Plant Samples	32
Total Samples	1285

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2399]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116268]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Carnobacterium alterfunditum large 16S-23S rRNA intergenic spacer, tRNA-Ile and tRNA-Ala genes, complete sequence	AY170834	448	ENA	28230 tax ID	*
[Ref.: #20218]	Carnobacterium alterfunditum medium 16S-23S rRNA intergenic spacer and tRNA-Ala gene, complete sequence	AY170835	337	ENA	28230 tax ID	*
[Ref.: #20218]	Carnobacterium alterfunditum small 16S-23S rRNA intergenic spacer, complete sequence	AY170836	225	ENA	28230 tax ID	*
[Ref.: #20218]	Carnobacterium alterfunditum gene for 16S rRNA, partial sequence, strain: NBRC 15548	AB680898	1484	ENA	28230 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Carnobacterium alterfunditum DSM 5972 pf4	GCA_000744115	contig	GenBank	1449335 tax ID	*
[Ref.: #66792]	Carnobacterium alterfunditum DSM 5972 strain pf4	1449335.3	wgs	PATRIC	1449335 tax ID	*
[Ref.: #66792]	Carnobacterium alterfunditum pf4	2574179752	draft	JGI IMG/M	28230 tax ID	*

[Ref.: #2399]

GC-content

33.3 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Carnobacterium alterfunditum DSM 5972 strain pf4 PATRIC: 1449335.3
[Ref.: #69481]	spore-forming	yes	75	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Carnobacterium alterfunditum DSM 5972 strain pf4 PATRIC: 1449335.3
[Ref.: #69480]	motile	no	55.223	no
[Ref.: #69480]	gram-positive	yes	88.391	no
[Ref.: #69480]	anaerobic	no	97.144	yes
[Ref.: #69480]	halophile	yes	85.982	no
[Ref.: #69480]	spore-forming	no	86.965	no
[Ref.: #69480]	thermophile	no	98.487	yes
[Ref.: #69480]	glucose-util	yes	89.587	no
[Ref.: #69480]	aerobic	no	91.686	no
[Ref.: #69480]	flagellated	no	79.805	no
[Ref.: #69480]	glucose-ferment	yes	75.877	no

Availability in culture collections External links

[Ref.: #2399]

Culture collection no.

DSM 5972, ACAM 313, ATCC 49837, CCUG 34643, IFO 15548, CIP 105796

[Ref.: #71825]

SI-ID 8234

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Psychrotrophic, lactic acid-producing bacteria from anoxic waters in Ace Lake, Antarctica; Carnobacterium funditum sp. nov. and Carnobacterium alterfunditum sp. nov.	Franzmann PD, Hopfl P, Weiss N, Tindall BJ	Arch Microbiol	10.1007/BF00262994	1991	*

References

#2399

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 5972

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40197 ; Curators of the CIP;
#52228 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 34643

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#71825

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID8234.1 )

#116268 Collection of Institut Pasteur ; Curators of the CIP; CIP 105796
* These data were automatically processed and therefore are not curated

Change proposal

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Carnobacterium alterfunditum DSM 5972 strain pf4

Carnobacterium alterfunditum DSM 5972 strain pf4

Change proposal

Change proposal