Name: Alloiococcus otitis DSM 7252
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2249

Type strain:

Species: Alloiococcus otitis

Culture col. no.: DSM 7252, ATCC 51267, NCFB 2890, CCUG 32997, CIP 103508

Strain history: CIP <- 1992, NCFB <- 1991, Upjohn Lab., Kalamazoo, MI, USA D.H. Batts and R.R Hinshaw

NCBI tax ID(s): 883081 (strain), 1652 (species)

SI-ID 10629

LMG 17751, ATCC 51267, CCM 4306, IFO 15545, NCFB 2890, CCUG 32997, CIP 103508, NCIMB 702890, NBRC 15545, DSM 7252, CNCTC 7328

Other strains from Alloiococcus otitis

A. otitis
A. otitis CCUG 44657
A. otitis CCUG 44658
A. otitis CCUG 44659

Section

Alloiococcus otitis DSM 7252 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from Human middle ear fluid,persistent otitis media.

Gram-positive
ovoid-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Carnobacteriaceae*
	Genus	*Alloiococcus*
	Species	**Alloiococcus otitis**
	Full Scientific Name (LPSN)	Alloiococcus otitis Aguirre and Collins 1992

Information on morphological and physiological properties Morphology

[Ref.: #23072]	Gram stain	positive
[Ref.: #121471]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #23072]	Cell shape	ovoid-shaped
[Ref.: #121471]	Cell shape	coccus-shaped

[Ref.: #121471]	Motility	yes
[Ref.: #69480]	Motility	no Confidence in %97.46

[Ref.: #23072]	Type of hemolysis	alpha
[Ref.: #23072]	Hemolysis ability	1
[Ref.: #23072]	Incubation period	2 days
[Ref.: #23072]	Cultivation medium used	blood agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3074]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #3074]

Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #3074]

Culture medium composition

expand / minimize

[Ref.: #23072]

Culture medium

blood agar

[Ref.: #23072]

Culture medium growth

[Ref.: #23072]

Culture medium

blood agar plates

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Culture medium growth

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Culture medium

Todd-Hewitt broth (Oxoid Ltd., Basingstoke, United Kingdom)

[Ref.: #23072]

Culture medium growth

[Ref.: #23072]

Culture medium composition

expand / minimize

[Ref.: #40680]

Culture medium

MEDIUM 6 - Columbia agar with 10 % horse blood

[Ref.: #40680]

Culture medium growth

[Ref.: #40680]

Culture medium composition

expand / minimize

[Ref.: #121471]

Culture medium

CIP Medium 6

[Ref.: #121471]

Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #3074]		growth	37 °C
[Ref.: #23072]	no	growth	45.0 °C
[Ref.: #40680]		growth	37 °C
[Ref.: #51306]		growth	37 °C
[Ref.: #121471]		growth	37 °C
[Ref.: #121471]	no	growth	10 °C
[Ref.: #121471]	no	growth	45 °C

[Ref.: #3074]	Temperature range	mesophilic
[Ref.: #23072]	Temperature range	thermophilic
[Ref.: #121471]	Temperature range	mesophilic
[Ref.: #121471]	Temperature range	psychrophilic
[Ref.: #121471]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23072]	Oxygen tolerance	aerobe
[Ref.: #121471]	Oxygen tolerance	obligate aerobe

[Ref.: #23072]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.998

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23072]		NaCl		growth	6.5	%
[Ref.: #121471]		NaCl	no	growth	6.5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23072]	arabinose ChEBI	-	builds acid from
[Ref.: #23072]	esculin ChEBI	-	hydrolysis
[Ref.: #121471]	esculin ChEBI	-	hydrolysis
[Ref.: #23072]	galactitol ChEBI	-	builds acid from
[Ref.: #23072]	glucose ChEBI	-	builds acid from
[Ref.: #23072]	glycerol ChEBI	-	builds acid from
[Ref.: #23072]	hippurate ChEBI	+/-	hydrolysis
[Ref.: #23072]	inulin ChEBI	-	builds acid from
[Ref.: #23072]	lactose ChEBI	-	builds acid from
[Ref.: #23072]	maltose ChEBI	-	builds acid from
[Ref.: #23072]	mannitol ChEBI	-	builds acid from
[Ref.: #23072]	mannose ChEBI	-	builds acid from
[Ref.: #121471]	nitrate ChEBI	-	reduction
[Ref.: #121471]	nitrite ChEBI	-	reduction
[Ref.: #23072]	raffinose ChEBI	-	builds acid from
[Ref.: #23072]	rhamnose ChEBI	-	builds acid from
[Ref.: #23072]	ribose ChEBI	-	builds acid from
[Ref.: #23072]	salicin ChEBI	-	builds acid from
[Ref.: #23072]	sorbitol ChEBI	-	builds acid from
[Ref.: #23072]	starch ChEBI	-	hydrolysis
[Ref.: #23072]	sucrose ChEBI	-	builds acid from
[Ref.: #23072]	trehalose ChEBI	-	builds acid from
[Ref.: #23072]	xylose ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #121471]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Enzyme	Enzyme activity	EC number
[Ref.: #121471]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #23072]	arginine dihydrolase	-	3.5.3.6
[Ref.: #23072]	beta-galactosidase	+	3.2.1.23
[Ref.: #23072]	catalase	+	1.11.1.6
[Ref.: #121471]	catalase	+	1.11.1.6
[Ref.: #23072]	cytochrome oxidase	-	1.9.3.1
[Ref.: #23072]	leucine arylamidase	+	3.4.11.1
[Ref.: #121471]	lysine decarboxylase	-	4.1.1.18
[Ref.: #121471]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #23072]	pyrrolidonyl arylamidase	+	3.4.19.3

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	butanoate fermentation	100	4 of 4
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	vitamin B1 metabolism	92.31	12 of 13
[Ref.: #66794]	palmitate biosynthesis	90.91	20 of 22
[Ref.: #66794]	pentose phosphate pathway	90.91	10 of 11
[Ref.: #66794]	chorismate metabolism	77.78	7 of 9
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	peptidoglycan biosynthesis	73.33	11 of 15
[Ref.: #66794]	NAD metabolism	72.22	13 of 18
[Ref.: #66794]	photosynthesis	71.43	10 of 14
[Ref.: #66794]	cardiolipin biosynthesis	71.43	5 of 7
[Ref.: #66794]	Entner Doudoroff pathway	70	7 of 10
[Ref.: #66794]	phenylalanine metabolism	69.23	9 of 13
[Ref.: #66794]	aspartate and asparagine metabolism	66.67	6 of 9
[Ref.: #66794]	selenocysteine biosynthesis	66.67	4 of 6
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	serine metabolism	66.67	6 of 9
[Ref.: #66794]	glycolysis	64.71	11 of 17
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
[Ref.: #66794]	pyrimidine metabolism	62.22	28 of 45
[Ref.: #66794]	purine metabolism	61.7	58 of 94
[Ref.: #66794]	methylglyoxal degradation	60	3 of 5
[Ref.: #66794]	phenylacetate degradation (aerobic)	60	3 of 5
[Ref.: #66794]	lipoate biosynthesis	60	3 of 5
[Ref.: #66794]	metabolism of amino sugars and derivatives	60	3 of 5
[Ref.: #66794]	flavin biosynthesis	60	9 of 15
[Ref.: #66794]	glutathione metabolism	57.14	8 of 14
[Ref.: #66794]	reductive acetyl coenzyme A pathway	57.14	4 of 7
[Ref.: #66794]	propanol degradation	57.14	4 of 7
[Ref.: #66794]	oxidative phosphorylation	57.14	52 of 91
[Ref.: #66794]	degradation of sugar alcohols	56.25	9 of 16
[Ref.: #66794]	CO2 fixation in Crenarchaeota	55.56	5 of 9
[Ref.: #66794]	metabolism of disaccharids	54.55	6 of 11
[Ref.: #66794]	alanine metabolism	51.72	15 of 29
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	threonine metabolism	50	5 of 10
[Ref.: #66794]	C4 and CAM-carbon fixation	50	4 of 8
[Ref.: #66794]	ribulose monophosphate pathway	50	1 of 2
[Ref.: #66794]	sulfopterin metabolism	50	2 of 4
[Ref.: #66794]	tetrahydrofolate metabolism	50	7 of 14
[Ref.: #66794]	cyclohexanol degradation	50	2 of 4
[Ref.: #66794]	suberin monomers biosynthesis	50	1 of 2
[Ref.: #66794]	starch degradation	50	5 of 10
[Ref.: #66794]	glutamate and glutamine metabolism	50	14 of 28
[Ref.: #66794]	non-pathway related	47.37	18 of 38
[Ref.: #66794]	proline metabolism	45.45	5 of 11
[Ref.: #66794]	lipid metabolism	45.16	14 of 31
[Ref.: #66794]	d-mannose degradation	44.44	4 of 9
[Ref.: #66794]	mevalonate metabolism	42.86	3 of 7
[Ref.: #66794]	tyrosine metabolism	42.86	6 of 14
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
[Ref.: #66794]	lysine metabolism	40.48	17 of 42
[Ref.: #66794]	arachidonate biosynthesis	40	2 of 5
[Ref.: #66794]	myo-inositol biosynthesis	40	4 of 10
[Ref.: #66794]	glycogen metabolism	40	2 of 5
[Ref.: #66794]	degradation of sugar acids	40	10 of 25
[Ref.: #66794]	cysteine metabolism	38.89	7 of 18
[Ref.: #66794]	methionine metabolism	38.46	10 of 26
[Ref.: #66794]	histidine metabolism	37.93	11 of 29
[Ref.: #66794]	ketogluconate metabolism	37.5	3 of 8
[Ref.: #66794]	isoleucine metabolism	37.5	3 of 8
[Ref.: #66794]	gluconeogenesis	37.5	3 of 8
[Ref.: #66794]	tryptophan metabolism	36.84	14 of 38
[Ref.: #66794]	d-xylose degradation	36.36	4 of 11
[Ref.: #66794]	citric acid cycle	35.71	5 of 14
[Ref.: #66794]	glycolate and glyoxylate degradation	33.33	2 of 6
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	degradation of hexoses	33.33	6 of 18
[Ref.: #66794]	octane oxidation	33.33	1 of 3
[Ref.: #66794]	formaldehyde oxidation	33.33	1 of 3
[Ref.: #66794]	arginine metabolism	33.33	8 of 24
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	urea cycle	30.77	4 of 13
[Ref.: #66794]	leucine metabolism	30.77	4 of 13
[Ref.: #66794]	glycine metabolism	30	3 of 10
[Ref.: #66794]	arachidonic acid metabolism	27.78	5 of 18
[Ref.: #66794]	vitamin B6 metabolism	27.27	3 of 11
[Ref.: #66794]	biotin biosynthesis	25	1 of 4
[Ref.: #66794]	degradation of pentoses	25	7 of 28
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	catecholamine biosynthesis	25	1 of 4
[Ref.: #66794]	CMP-KDO biosynthesis	25	1 of 4
[Ref.: #66794]	isoprenoid biosynthesis	23.08	6 of 26
[Ref.: #66794]	nitrate assimilation	22.22	2 of 9
[Ref.: #66794]	valine metabolism	22.22	2 of 9
[Ref.: #66794]	lipid A biosynthesis	22.22	2 of 9
[Ref.: #66794]	molybdenum cofactor biosynthesis	22.22	2 of 9
[Ref.: #66794]	4-hydroxymandelate degradation	22.22	2 of 9
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3074]	Sample type/isolated from	Human middle ear fluid,persistent otitis media

[Ref.: #51306]	Sample type/isolated from	Human middle ear

[Ref.: #121471]	Sample type/isolated from	Human, Child, otitis
[Ref.: #121471]	Isolation date	1989
* marker position based on {}

Isolation sources categories

#Infection	#Disease	-
#Host	#Human	-
#Host Body-Site	#Organ	#Ear
#Host Body Product	#Fluids	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB680896 (>99% sequence identity) for Alloiococcus otitis subclade from Microbeatlas

Aquatic Samples	215
Soil Samples	37
Animal Samples	2688
Plant Samples	81
Total Samples	3021

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3074]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #121471]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Alloiococcus otitis gene for 16S rRNA, partial sequence, strain: NBRC 15545	AB680896	1474	ENA	1652 tax ID	*
[Ref.: #20218]	A.otitis 16S rRNA	X59765	1525	ENA	1652 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Alloiococcus otitis ATCC 51267	GCA_000315445	scaffold	GenBank	883081 tax ID	*
[Ref.: #66792]	Alloiococcus otitis ATCC 51267	883081.3	wgs	PATRIC	883081 tax ID	*
[Ref.: #66792]	Alloiococcus otitis ATCC 51267	2537561904	draft	JGI IMG/M	883081 tax ID	*

[Ref.: #3074]	GC-content	44.0 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #23072]	GC-content	44.0-45.0 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Alloiococcus otitis ATCC 51267 ATCC 51267 NCBI: GCA_000315445.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Alloiococcus otitis ATCC 51267 ATCC 51267 NCBI: GCA_000315445.1
[Ref.: #69480]	motile	no	95.914	no
[Ref.: #69480]	flagellated	no	98.448	no
[Ref.: #69480]	gram-positive	yes	94.697	yes
[Ref.: #69480]	anaerobic	no	94.404	yes
[Ref.: #69480]	aerobic	no	89.696	no
[Ref.: #69480]	halophile	yes	97.71	yes
[Ref.: #69480]	spore-forming	no	96.595	no
[Ref.: #69480]	thermophile	no	97.483	no
[Ref.: #69480]	glucose-util	yes	87.21	no
[Ref.: #69480]	glucose-ferment	yes	80.13	no

Availability in culture collections External links

[Ref.: #3074]

Culture collection no.

DSM 7252, ATCC 51267, NCFB 2890, CCUG 32997, CIP 103508

[Ref.: #71808]

SI-ID 10629

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Phylogenetic analysis of Alloiococcus otitis gen. nov., sp. nov., an organism from human middle ear fluid.	Aguirre M, Collins MD	Int J Syst Bacteriol	10.1099/00207713-42-1-79	1992	*

References

#3074

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7252

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23072

M. Aguirre,M. D. Collins: Phylogenetic Analysis of Alloiococcus otitis gen. nov., sp. nov., an Organism from Human Middle Ear Fluid. IJSEM 42: 79 - 83 1992 ( DOI 10.1099/00207713-42-1-79 , PubMed 1371065 )

#40680 ; Curators of the CIP;
#51306 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 32997

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#71808

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID10629.1 )

#121471 Collection of Institut Pasteur ; Curators of the CIP; CIP 103508
* These data were automatically processed and therefore are not curated

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Alloiococcus otitis ATCC 51267 ATCC 51267

Alloiococcus otitis ATCC 51267 ATCC 51267

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