Alloiococcus otitis (DSM 7252, ATCC 51267, NCFB 2890)

Name: Alloiococcus otitis (DSM 7252, ATCC 51267, NCFB 2890)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2249

Type strain

Culture col. no. DSM 7252 ATCC 51267 NCFB 2890 CCUG 32997 CIP 103508 3 more

NCBI tax ID(s) 883081 1652

Special Collections DSMZ CCUG Literature strains CIP

History <- NCFB; NCFB 2890 CIP <- 1992, NCFB <- 1991, Upjohn Lab., Kalamazoo, MI, USA D.H. Batts and R.R Hinshaw

Links

Alloiococcus otitis DSM 7252 is an aerobe, Gram-positive, ovoid-shaped bacterium that was isolated from Human middle ear fluid,persistent otitis media.

Gram-positive ovoid-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 10629

LMG 17751,ATCC 51267,CCM 4306,IFO 15545,NCFB 2890,NCTC 13840,CCUG 32997,CIP 103508,NCIMB 702890,NBRC 15545,DSM 7252,CNCTC 7328

@ref 20215

Last LPSN update 2026-02-09 11:18:15

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Carnobacteriaceae

Genus Alloiococcus

Species Alloiococcus otitis

Full scientific name Alloiococcus otitis Aguirre and Collins 1992

BacDive ID	Other strains from **Alloiococcus otitis** (4)	Type strain
147174	A. otitis
151694	A. otitis CCUG 44657
151695	A. otitis CCUG 44658
151696	A. otitis CCUG 44659

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
23072	positive	ovoid-shaped
121471	positive	coccus-shaped
125438			90.597
125438	positive		90.497

Colony morphology

@ref	Type of hemolysis	Hemolysis ability	Incubation period	Medium used
23072	alpha	1	2 days	blood agar
121471

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
3074	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
23072	blood agar
23072	blood agar plates
23072	Todd-Hewitt broth (Oxoid Ltd., Basingstoke, United Kingdom)		supplemented with 5% horse serum
40680	MEDIUM 6 - Columbia agar with 10 % horse blood		Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
121471	CIP Medium 6	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
3074	positive	growth	37
23072	negative	growth	45.0
40680	positive	growth	37
51306	positive	growth	37
121471	positive	growth	37
121471	negative	growth	10
121471	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
23072	aerobe
121471	obligate aerobe
125439	anaerobe	91.2

Spore formation

@ref	Spore formation	Confidence
23072
125439		99.5

Halophily

@ref	Salt	Growth	Tested relation	Concentration
23072	NaCl	positive	growth	6.5 %
121471	NaCl		growth	6.5 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23072	22599 ChEBI	arabinose	-	builds acid from
68375	22599 ChEBI	arabinose	-	fermentation	from API ID32STA
68375	29016 ChEBI	arginine	-	hydrolysis	from API ID32STA
68375	17057 ChEBI	cellobiose	-	fermentation	from API ID32STA
68375	16899 ChEBI	D-mannitol	-	fermentation	from API ID32STA
68375	16024 ChEBI	D-mannose	-	fermentation	from API ID32STA
68375	16988 ChEBI	D-ribose	-	fermentation	from API ID32STA
23072	4853 ChEBI	esculin	-	hydrolysis
68375	4853 ChEBI	esculin	-	hydrolysis	from API ID32STA
121471	4853 ChEBI	esculin	-	hydrolysis
23072	16813 ChEBI	galactitol	-	builds acid from
23072	17234 ChEBI	glucose	-	builds acid from
23072	17754 ChEBI	glycerol	-	builds acid from
23072	606565 ChEBI	hippurate	+/-	hydrolysis
23072	15443 ChEBI	inulin	-	builds acid from
23072	17716 ChEBI	lactose	-	builds acid from
68375	17716 ChEBI	lactose	-	fermentation	from API ID32STA
23072	17306 ChEBI	maltose	-	builds acid from
68375	17306 ChEBI	maltose	-	fermentation	from API ID32STA
23072	29864 ChEBI	mannitol	-	builds acid from
23072	37684 ChEBI	mannose	-	builds acid from
68375	17632 ChEBI	nitrate	-	reduction	from API ID32STA
121471	17632 ChEBI	nitrate	-	reduction
121471	16301 ChEBI	nitrite	-	reduction
68375	18257 ChEBI	ornithine	-	degradation	from API ID32STA
23072	16634 ChEBI	raffinose	-	builds acid from
68375	16634 ChEBI	raffinose	-	fermentation	from API ID32STA
23072	26546 ChEBI	rhamnose	-	builds acid from
23072	33942 ChEBI	ribose	-	builds acid from
23072	17814 ChEBI	salicin	-	builds acid from
23072	30911 ChEBI	sorbitol	-	builds acid from
23072	28017 ChEBI	starch	-	hydrolysis
23072	17992 ChEBI	sucrose	-	builds acid from
68375	17992 ChEBI	sucrose	-	fermentation	from API ID32STA
23072	27082 ChEBI	trehalose	-	builds acid from
68375	27082 ChEBI	trehalose	-	fermentation	from API ID32STA
68375	32528 ChEBI	turanose	-	fermentation	from API ID32STA
68375	16199 ChEBI	urea	-	hydrolysis	from API ID32STA
23072	18222 ChEBI	xylose	-	builds acid from

Antibiotic resistance

@ref	ChEBI	Metabolite	Is sensitive	Sensitivity conc.	Is resistant
121471		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
68375	28368	novobiocin		1.8 µg		from API ID32STA

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68375	15688 ChEBI	acetoin		from API ID32STA

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
68375	15688 ChEBI	acetoin	-	from API ID32STA

Enzymes

@ref	Value	Activity	Ec
121471	alcohol dehydrogenase	-	1.1.1.1
68375	alkaline phosphatase	-	3.1.3.1	from API ID32STA
23072	arginine dihydrolase	-	3.5.3.6
68375	arginine dihydrolase	-	3.5.3.6	from API ID32STA
23072	beta-galactosidase	+	3.2.1.23
68375	beta-galactosidase	+	3.2.1.23	from API ID32STA
68375	beta-glucosidase	-	3.2.1.21	from API ID32STA
68375	beta-glucuronidase	-	3.2.1.31	from API ID32STA
23072	catalase	+	1.11.1.6
121471	catalase	+	1.11.1.6
23072	cytochrome oxidase	-	1.9.3.1
68375	L-arginine arylamidase	+		from API ID32STA
23072	leucine arylamidase	+	3.4.11.1
121471	lysine decarboxylase	-	4.1.1.18
121471	ornithine decarboxylase	-	4.1.1.17
68375	ornithine decarboxylase	-	4.1.1.17	from API ID32STA
23072	pyrrolidonyl arylamidase	+	3.4.19.3
68375	pyrrolidonyl arylamidase	+	3.4.19.3	from API ID32STA
68375	urease	-	3.5.1.5	from API ID32STA

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	adipate degradation	100	2 of 2
66794	ppGpp biosynthesis	100	4 of 4
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	butanoate fermentation	100	4 of 4
66794	coenzyme A metabolism	100	4 of 4
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	vitamin B1 metabolism	92.31	12 of 13
66794	palmitate biosynthesis	90.91	20 of 22
66794	pentose phosphate pathway	90.91	10 of 11
66794	chorismate metabolism	77.78	7 of 9
66794	acetate fermentation	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	peptidoglycan biosynthesis	73.33	11 of 15
66794	NAD metabolism	72.22	13 of 18
66794	photosynthesis	71.43	10 of 14
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	Entner Doudoroff pathway	70	7 of 10
66794	phenylalanine metabolism	69.23	9 of 13
66794	aspartate and asparagine metabolism	66.67	6 of 9
66794	selenocysteine biosynthesis	66.67	4 of 6
66794	acetoin degradation	66.67	2 of 3
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	serine metabolism	66.67	6 of 9
66794	glycolysis	64.71	11 of 17
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
66794	pyrimidine metabolism	62.22	28 of 45
66794	purine metabolism	61.7	58 of 94
66794	methylglyoxal degradation	60	3 of 5
66794	phenylacetate degradation (aerobic)	60	3 of 5
66794	lipoate biosynthesis	60	3 of 5
66794	metabolism of amino sugars and derivatives	60	3 of 5
66794	flavin biosynthesis	60	9 of 15
66794	glutathione metabolism	57.14	8 of 14
66794	reductive acetyl coenzyme A pathway	57.14	4 of 7
66794	propanol degradation	57.14	4 of 7
66794	oxidative phosphorylation	57.14	52 of 91
66794	degradation of sugar alcohols	56.25	9 of 16
66794	CO2 fixation in Crenarchaeota	55.56	5 of 9
66794	metabolism of disaccharids	54.55	6 of 11
66794	alanine metabolism	51.72	15 of 29
66794	ethanol fermentation	50	1 of 2
66794	threonine metabolism	50	5 of 10
66794	C4 and CAM-carbon fixation	50	4 of 8
66794	ribulose monophosphate pathway	50	1 of 2
66794	sulfopterin metabolism	50	2 of 4
66794	tetrahydrofolate metabolism	50	7 of 14
66794	cyclohexanol degradation	50	2 of 4
66794	suberin monomers biosynthesis	50	1 of 2
66794	starch degradation	50	5 of 10
66794	glutamate and glutamine metabolism	50	14 of 28
66794	non-pathway related	47.37	18 of 38
66794	proline metabolism	45.45	5 of 11
66794	lipid metabolism	45.16	14 of 31
66794	d-mannose degradation	44.44	4 of 9
66794	mevalonate metabolism	42.86	3 of 7
66794	tyrosine metabolism	42.86	6 of 14
66794	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
66794	lysine metabolism	40.48	17 of 42
66794	arachidonate biosynthesis	40	2 of 5
66794	myo-inositol biosynthesis	40	4 of 10
66794	glycogen metabolism	40	2 of 5
66794	degradation of sugar acids	40	10 of 25
66794	cysteine metabolism	38.89	7 of 18
66794	methionine metabolism	38.46	10 of 26
66794	histidine metabolism	37.93	11 of 29
66794	ketogluconate metabolism	37.5	3 of 8
66794	isoleucine metabolism	37.5	3 of 8
66794	gluconeogenesis	37.5	3 of 8
66794	tryptophan metabolism	36.84	14 of 38
66794	d-xylose degradation	36.36	4 of 11
66794	citric acid cycle	35.71	5 of 14
66794	glycolate and glyoxylate degradation	33.33	2 of 6
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	degradation of hexoses	33.33	6 of 18
66794	octane oxidation	33.33	1 of 3
66794	formaldehyde oxidation	33.33	1 of 3
66794	arginine metabolism	33.33	8 of 24
66794	IAA biosynthesis	33.33	1 of 3
66794	urea cycle	30.77	4 of 13
66794	leucine metabolism	30.77	4 of 13
66794	glycine metabolism	30	3 of 10
66794	arachidonic acid metabolism	27.78	5 of 18
66794	vitamin B6 metabolism	27.27	3 of 11
66794	biotin biosynthesis	25	1 of 4
66794	degradation of pentoses	25	7 of 28
66794	toluene degradation	25	1 of 4
66794	catecholamine biosynthesis	25	1 of 4
66794	CMP-KDO biosynthesis	25	1 of 4
66794	isoprenoid biosynthesis	23.08	6 of 26
66794	nitrate assimilation	22.22	2 of 9
66794	valine metabolism	22.22	2 of 9
66794	lipid A biosynthesis	22.22	2 of 9
66794	molybdenum cofactor biosynthesis	22.22	2 of 9
66794	4-hydroxymandelate degradation	22.22	2 of 9

API ID32STA

@ref	URE	ADH (Arg)	ODC	ESC	GLU	FRU	MNE	MAL	LAC	TRE	MAN	RAF	RIB	CEL	Reduction of nitrateNIT	Acetoin production (Voges Proskauer test)VP	beta GAL	L-arginine arylamidaseArgA	PAL	Pyrrolidonyl arylamidasePyrA	NOVO	SAC	NAG	TUR	ARA	beta GUR
3074	-	-	-	-	+/-	+/-	-	-	-	-	-	-	-	-	-	-	+	+	-	+	-	-	+/-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Infection	#Disease	-
#Host	#Human	-
#Host Body-Site	#Organ	#Ear
#Host Body Product	#Fluids	-

Isolation

@ref	Sample type	Isolation date
3074	Human middle ear fluid,persistent otitis media
51306	Human middle ear
121471	Human, Child, otitis	1989

Taxonmaps

69479

Global distribution of 16S sequence AB680896 (>99% sequence identity) for Alloiococcus otitis subclade from Microbeatlas

Aquatic Samples	215
Soil Samples	37
Animal Samples	2688
Plant Samples	81
Total Samples	3021

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
3074	2	Risk group (German classification) According to TRBA 466
121471	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	Allo_otit_ATCC_51267_V1 assembly for Alloiococcus otitis ATCC 51267	scaffold	GCA_000315445	883081.3	2537561904	883081	70.11

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Alloiococcus otitis gene for 16S rRNA, partial sequence, strain: NBRC 15545	AB680896	1474	1652
20218	A.otitis 16S rRNA	X59765	1525	1652
124043	Alloiococcus otitis strain CCUG 32997 16S-23S ribosomal RNA intergenic spacer, complete sequence.	JQ012769	227	1652

GC content

@ref	GC-content (mol%)	Method
3074	44.0	thermal denaturation, midpoint method (Tm)
23072	44.0-45.0

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Alloiococcus otitis ATCC 51267
NCBI: GCA_000315445

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	99.50	no
125439	motility	BacteriaNetⓘ	no	80.90	no
125439	gram_stain	BacteriaNetⓘ	negative	86.30	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	91.20	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Alloiococcus otitis ATCC 51267 ATCC 51267
NCBI: GCA_000315445.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	90.50	yes
125438	anaerobic	anaerobicⓘ	no	86.73	no
125438	aerobic	aerobicⓘ	no	64.82	yes
125438	spore-forming	spore-formingⓘ	no	84.15	yes
125438	thermophilic	thermophileⓘ	no	93.92	no
125438	flagellated	motile2+ⓘ	no	90.60	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genomic Stability and Genetic Defense Systems in Dolosigranulum pigrum, a Candidate Beneficial Bacterium from the Human Microbiome.	Flores Ramos S, Brugger SD, Escapa IF, Skeete CA, Cotton SL, Eslami SM, Gao W, Bomar L, Tran TH, Jones DS, Minot S, Roberts RJ, Johnston CD, Lemon KP.	mSystems	10.1128/msystems.00425-21	2021
	Development of a polymerase chain reaction-probe test for identification of Alloiococcus otitis.	Aguirre M, Collins MD.	J Clin Microbiol	10.1128/jcm.30.8.2177-2180.1992	1992
Phylogeny	Phylogenetic analysis of Alloiococcus otitis gen. nov., sp. nov., an organism from human middle ear fluid.	Aguirre M, Collins MD	Int J Syst Bacteriol	10.1099/00207713-42-1-79	1992

References

#3074	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7252
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#23072	M. Aguirre,M. D. Collins: Phylogenetic Analysis of Alloiococcus otitis gen. nov., sp. nov., an Organism from Human Middle Ear Fluid. IJSEM 42: 79 - 83 1992 ( DOI 10.1099/00207713-42-1-79 , PubMed 1371065 )
#40680	; Curators of the CIP;
#51306	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 32997
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#68375	Automatically annotated from API ID32STA .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#121471	Collection of Institut Pasteur ; Curators of the CIP; CIP 103508
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Alloiococcus otitis (DSM 7252, ATCC 51267, NCFB 2890)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

Pathway annotation

API ID32STA

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000315445

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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