Allofustis seminis (DSM 15817, CCUG 45438, CIP 107425)

Name: Allofustis seminis (DSM 15817, CCUG 45438, CIP 107425)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2248

Type strain

Strain Designation 01-570-1

Culture col. no. DSM 15817 CCUG 45438 CIP 107425 01-570-1

NCBI tax ID(s) 1120978 166939

Special Collections DSMZ CCUG CIP

History <- E. Falsen, CCUG <- M. D. Collins, Univ. of Reading <- R. Higgins CIP <- 2002, CCUG <- M.D. Collins, Univ. Reading, Reading, UK: strain 01-570-1

Links

Allofustis seminis 01-570-1 is an anaerobe, Gram-positive, rod-shaped bacterium that was isolated from stored porcine semen.

Gram-positive rod-shaped anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 87828

CCUG 45438,CIP 107425,DSM 15817

@ref 20215

Last LPSN update 2026-02-09 11:18:15

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Carnobacteriaceae

Genus Allofustis

Species Allofustis seminis

Full scientific name Allofustis seminis Collins et al. 2003

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
122555	positive	rod-shaped
125438			91.386
125438	positive		90.423

Colony morphology

@ref	Incubation period
56786	2-3 days
122555

Multimedia

@ref:	66793
multimedia content:	EM_DSM_15817_1.jpg
multimedia.multimedia content:	EM_DSM_15817_1.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_15817_2.jpg
multimedia.multimedia content:	EM_DSM_15817_2.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_15817_3.jpg
multimedia.multimedia content:	EM_DSM_15817_3.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_15817_4.jpg
multimedia.multimedia content:	EM_DSM_15817_4.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_15817_5.jpg
multimedia.multimedia content:	EM_DSM_15817_5.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
6121	PYG MEDIUM (MODIFIED) (DSMZ Medium 104)	Medium recipe at MediaDive	Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
6121	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
33126	MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and Neisseria		Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml);PolyVitex mischung (10.000 ml)
122555	CIP Medium 10	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
6121	positive	growth	37
33126	positive	growth	37
56786	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
6121	anaerobe
56786	anaerobe
56786	microaerophile

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
122555	17632 ChEBI	nitrate	-	reduction
122555	17632 ChEBI	nitrate	-	respiration
122555	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
122555	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
122555	amylase	-
68382	beta-galactosidase	-	3.2.1.23	from API zym
122555	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
122555	caseinase	-	3.4.21.50
122555	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
122555	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	-		from API zym
122555	gelatinase	-
122555	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
122555	lipase	-
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
122555	oxidase	-
122555	protease	-
68382	trypsin	-	3.4.21.4	from API zym
122555	tween esterase	-
122555	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
122555	-	-	+	-	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Mammals	#Suidae (Pig,Swine)
#Host Body Product	#Urogenital tract	#Semen

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
6121	stored porcine semen	Montreal	Canada	CAN	North America
56786	Porcine semen,environmental contaminant ?	Montreal	Canada	CAN	North America
122555	Animal, Porcine semen		United Kingdom	GBR	Europe

Taxonmaps

69479

Global distribution of 16S sequence AJ410303 (>99% sequence identity) for Allofustis seminis subclade from Microbeatlas

Aquatic Samples	120
Soil Samples	105
Animal Samples	966
Plant Samples	48
Total Samples	1239

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6121	1	Risk group (German classification) According to TRBA 466
122555	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM37432v1 assembly for Allofustis seminis DSM 15817	scaffold	GCA_000374325	1120978.3	2518285549	1120978	74.9

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Allofustis seminis 16S rRNA gene, type strain CCUG 45438T	AJ458446	1500		166939
6121	Alloiococcus sp. CCUG 45438 16S rRNA gene, strain CCUG 45438	AJ410303	1500		166939

GC content

6121

GC-content (mol%)39

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Allofustis seminis DSM 15817
NCBI: GCA_000374325

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	71.00	no
125439	motility	BacteriaNetⓘ	no	56.60	no
125439	gram_stain	BacteriaNetⓘ	positive	74.60	no
125439	oxygen_tolerance	BacteriaNetⓘ	microaerophile	87.60	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Allofustis seminis DSM 15817 DSM 15817
NCBI: GCA_000374325.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	90.42	no
125438	anaerobic	anaerobicⓘ	no	78.85	no
125438	aerobic	aerobicⓘ	no	96.22	yes
125438	spore-forming	spore-formingⓘ	no	86.55	no
125438	thermophilic	thermophileⓘ	no	91.62	yes
125438	flagellated	motile2+ⓘ	no	91.39	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Allofustis seminis gen. nov., sp. nov., a novel Gram-positive, catalase-negative, rod-shaped bacterium from pig semen.	Collins MD, Higgins R, Messier S, Fortin M, Hutson RA, Lawson PA, Falsen E	Int J Syst Evol Microbiol	10.1099/ijs.0.02484-0	2003

References

#6121	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15817
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#33126	; Curators of the CIP;
#56786	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 45438
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66793	Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#122555	Collection of Institut Pasteur ; Curators of the CIP; CIP 107425
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Allofustis seminis (DSM 15817, CCUG 45438, CIP 107425)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Metabolite utilization

Metabolite production

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000374325

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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