Name: Trueperella bonasi 2W106/04
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 217

Type strain:

Species: Trueperella bonasi

Strain Designation: 2W106/04, W106/04

Culture col. no.: CCUG 59861, DSM 17163, CIP 109232, NCTC 13355

Strain history: CIP <- 2006, DSMZ <- A. Lehnen, Inst. für Zoo, Berlin, Germany: strain W106/04

NCBI tax ID(s): 312286 (species)

SI-ID 290959

DSM 17163, NCTC 13355, CIP 109232, CCUG 59861

Section

Trueperella bonasi 2W106/04 is a microaerophile, mesophilic, coccus-shaped animal pathogen that forms circular colonies and was isolated from prepuce of European bison Bison bonasus suffering from balanoposthitis.

coccus-shaped
colony-forming
animal pathogen
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Actinomycetales*
	Family	*Actinomycetaceae*
	Genus	*Trueperella*
	Species	**Trueperella bonasi**
	Full Scientific Name (LPSN)	Trueperella bonasi (Lehnen et al. 2006) Yassin et al. 2011

Synonym

Arcanobacterium bonasi

Information on morphological and physiological properties Morphology

[Ref.: #31626]	Gram stain	positive
[Ref.: #116838]	Gram stain	positive

[Ref.: #31626]	Cell shape	rod-shaped
[Ref.: #116838]	Cell shape	oval-shaped
[Ref.: #22976]	Cell shape	coccus-shaped

[Ref.: #31626]	Motility	no
[Ref.: #116838]	Motility	no
[Ref.: #22976]	Motility	no

[Ref.: #22976]	Cell length	0.3-2.5 µm

[Ref.: #22976]	Cell width	0.2-0.9 µm

[Ref.: #22976]	Type of hemolysis	beta
[Ref.: #22976]	Hemolysis ability	1
[Ref.: #22976]	Colony size	0.5-1.5 mm
[Ref.: #22976]	Colony color	translucent
[Ref.: #22976]	Colony shape	circular
[Ref.: #22976]	Incubation period	2-3 days

[Ref.: #62137]	Incubation period	2-3 days

[Ref.: #31626]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6798]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #6798]

Culture medium growth

[Ref.: #6798]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6798]

Culture medium composition

expand / minimize

[Ref.: #40027]

Culture medium

MEDIUM 45 - for Columbia agar with sheep blood

[Ref.: #40027]

Culture medium growth

[Ref.: #40027]

Culture medium composition

expand / minimize

[Ref.: #116838]

Culture medium

CIP Medium 45

[Ref.: #116838]

Culture medium growth

[Ref.: #116838]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #6798]	growth	37 °C
[Ref.: #31626]	growth	37 °C
[Ref.: #31626]	optimum	37 °C
[Ref.: #40027]	growth	37 °C
[Ref.: #62137]	growth	37 °C

[Ref.: #6798]	Temperature range	mesophilic
[Ref.: #31626]	Temperature range	mesophilic
[Ref.: #40027]	Temperature range	mesophilic
[Ref.: #62137]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31626]			optimum	7
[Ref.: #31626]			growth	7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #6798]	Oxygen tolerance	microaerophile
[Ref.: #6798]	Oxygen tolerance	aerobe
[Ref.: #31626]	Oxygen tolerance	facultative anaerobe
[Ref.: #62137]	Oxygen tolerance	microaerophile

[Ref.: #22976]	Ability of spore formation	no
[Ref.: #31626]	Ability of spore formation	no

[Ref.: #22976]	Murein short key	A11.51
[Ref.: #22976]	Murein types	A5alpha L-Lys-L-Lys-D-Glu

[Ref.: #31626]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #22976]	D-glucose ChEBI	-	builds acid from
[Ref.: #22976]	D-mannitol ChEBI	-	builds acid from
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #22976]	D-ribose ChEBI	-	builds acid from
[Ref.: #22976]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #22976]	D-xylose ChEBI	-	builds acid from
[Ref.: #22976]	gelatin ChEBI	-	hydrolysis
[Ref.: #22976]	glycerol ChEBI	-	builds acid from
[Ref.: #31626]	glycerol ChEBI	+	carbon source
[Ref.: #22976]	glycogen ChEBI	-	builds acid from
[Ref.: #22976]	hippurate ChEBI	+	hydrolysis
[Ref.: #22976]	L-arabinose ChEBI	-	builds acid from
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #22976]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #22976]	lactose ChEBI	-	builds acid from
[Ref.: #31626]	lactose ChEBI	+	carbon source
[Ref.: #22976]	maltose ChEBI	-	builds acid from
[Ref.: #31626]	maltose ChEBI	+	carbon source
[Ref.: #22976]	mannose ChEBI	-	builds acid from
[Ref.: #116838]	nitrate ChEBI	-	reduction
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #116838]	nitrite ChEBI	-	reduction
[Ref.: #22976]	raffinose ChEBI	-	builds acid from
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #22976]	salicin ChEBI	-	builds acid from
[Ref.: #22976]	sucrose ChEBI	-	builds acid from
[Ref.: #22976]	trehalose ChEBI	-	builds acid from
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #22976]	acetoin ChEBI	no
[Ref.: #116838]	indole ChEBI	no
[Ref.: #68380]	indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #22976]	acid phosphatase	+	3.1.3.2
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68380]	alanine arylamidase	+	3.4.11.2	* from API rID32A
[Ref.: #22976]	alkaline phosphatase	-	3.1.3.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym, API rID32A
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym, API rID32A
[Ref.: #22976]	alpha-galactosidase	-	3.2.1.22
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym, API rID32A
[Ref.: #22976]	alpha-glucosidase	-	3.2.1.20
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym, API rID32A
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #22976]	arginine dihydrolase	-	3.5.3.6
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #22976]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym, API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #22976]	beta-glucosidase	-	3.2.1.21
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym, API rID32A
[Ref.: #22976]	beta-glucuronidase	+	3.2.1.31
[Ref.: #68382]	beta-glucuronidase	+	3.2.1.31	* from API zym, API rID32A
[Ref.: #22976]	catalase	-	1.11.1.6
[Ref.: #31626]	catalase	+	1.11.1.6
[Ref.: #116838]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #22976]	esterase lipase (C 8)	+
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	glycin arylamidase	+		* from API rID32A
[Ref.: #68380]	histidine arylamidase	+		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	+		* from API rID32A
[Ref.: #22976]	leucine arylamidase	-	3.4.11.1
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym, API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	+	3.4.11.1	* from API rID32A
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #22976]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym, API rID32A
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #22976]	nitrate reductase	-	1.7.99.4
[Ref.: #116838]	oxidase	-
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #22976]	pyrazinamidase	+	3.5.1.B15
[Ref.: #22976]	pyrrolidonyl arylamidase	+	3.4.19.3
[Ref.: #68380]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #22976]	urease	-	3.5.1.5
[Ref.: #116838]	urease	-	3.5.1.5
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #6798]	12641	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	+	-	+	-	+	+	-	+	+	+	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116838]	11734	-	-	+	+	-	+	-	-	-	-	-	-	-	-	+	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6798]	Sample type/isolated from	prepuce of European bison Bison bonasus suffering from balanoposthitis
[Ref.: #6798]	Host species	Bison bonasus
[Ref.: #6798]	Geographic location (country and/or sea, region)	Bialowieza National Park
[Ref.: #6798]	Country	Poland
[Ref.: #6798]	Country ISO 3 Code	POL
[Ref.: #6798]	Continent	Europe

[Ref.: #62137]	Sample type/isolated from	Prepuce of European bison
[Ref.: #62137]	Country	Poland
[Ref.: #62137]	Country ISO 3 Code	POL
[Ref.: #62137]	Continent	Europe

[Ref.: #116838]	Sample type/isolated from	European bison, prepuce
[Ref.: #116838]	Country	Poland
[Ref.: #116838]	Country ISO 3 Code	POL
[Ref.: #116838]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body-Site	#Urogenital tract	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ879697 (>99% sequence identity) for Trueperella bonasi subclade from Microbeatlas

Aquatic Samples	4
Soil Samples	14
Animal Samples	97
Plant Samples
Total Samples	115

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6798]	Pathogenicity (animal)	yes
[Ref.: #6798]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #116838]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6798]	Arcanobacterium bonasi 16S rRNA gene, type strain: 2(W106/04)T	AJ879697	1445	ENA	312286 tax ID
[Ref.: #20218]	Arcanobacterium bonasi strain DSM 17163 16S ribosomal RNA gene and 16S-23S ribosomal RNA intergenic spacer, partial sequence	EU194570	512	ENA	312286 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Trueperella bonasi DSM 17163	2923644855	draft	JGI IMG/M	312286 tax ID	*

[Ref.: #6798]

GC-content

58.1 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #6798]

Culture collection no.

CCUG 59861, DSM 17163, CIP 109232, NCTC 13355

[Ref.: #69900]

SI-ID 290959

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Molecular identification of Arcanobacterium bialowiezense and Arcanobacterium bonasi based on 16S-23S rRNA intergenic spacer region sequences.	Hassan AA, Mohyla H, Kanbar T, Alber J, Lammler C, Abdulmawjood A, Speck S, Zschock M, Weiss R	Vet Microbiol	10.1016/j.vetmic.2008.02.008	2008	*
Phylogeny	Arcanobacterium bialowiezense sp. nov. and Arcanobacterium bonasi sp. nov., isolated from the prepuce of European bison bulls (Bison bonasus) suffering from balanoposthitis, and emended description of the genus Arcanobacterium Collins et al. 1983.	Lehnen A, Busse HJ, Frolich K, Krasinska M, Kampfer P, Speck S	Int J Syst Evol Microbiol	10.1099/ijs.0.63923-0	2006	*

References

#6798

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17163

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#22976

A. F. Yassin,H. Hupfer,C. Siering,P. Schumann: Comparative chemotaxonomic and phylogenetic studies on the genus Arcanobacterium Collins et al. 1982 emend. Lehnen et al. 2006: proposal for Trueperella gen. nov. and emended description of the genus Arcanobacterium. IJSEM 61: 1265 - 1274 2011 ( DOI 10.1099/ijs.0.020032-0 , PubMed 20622055 )

#31626

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27911 (see below)

#40027 ; Curators of the CIP;
#62137 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 59861

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68380

Automatically annotated from API rID32A .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69900

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID290959.1 )

#116838 Collection of Institut Pasteur ; Curators of the CIP; CIP 109232

#27911

IJSEM 861 2006 ( DOI 10.1099/ijs.0.63923-0 , PubMed 16585707 )

* These data were automatically processed and therefore are not curated

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