Name: Campylobacter cuniculorum 150B
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2140

Type strain:

Species: Campylobacter cuniculorum

Strain Designation: 150B

Culture col. no.: CIP 110020, DSM 23162, CCUG 56289, LMG 24588

Strain history: CIP <- 2009, CCUG <- M. Rossi, Bologna Univ., Bologna, Italy: strain 150B

NCBI tax ID(s): 1121267 (strain), 374106 (species)

SI-ID 292326

LMG 24588, CCUG 56289, CIP 110020, DSM 23162

Section

Campylobacter cuniculorum 150B is a microaerophile, mesophilic, Gram-negative animal pathogen that was isolated from rabbit caecal contents Oryctolagus cuniculus.

Gram-negative
motile
rod-shaped
animal pathogen
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Epsilonproteobacteria*
	Order	*Campylobacterales*
	Family	*Campylobacteraceae*
	Genus	*Campylobacter*
	Species	**Campylobacter cuniculorum**
	Full Scientific Name (LPSN)	Campylobacter cuniculorum Zanoni et al. 2009

Information on morphological and physiological properties Morphology

[Ref.: #29146]	Gram stain	negative
[Ref.: #118817]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.998

[Ref.: #29146]	Cell length	1.9-3.3 µm

[Ref.: #29146]	Cell width	0.2-0.4 µm

[Ref.: #29146]	Cell shape	rod-shaped
[Ref.: #118817]	Cell shape	rod-shaped

[Ref.: #29146]	Motility	yes
[Ref.: #118817]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %92.821

[Ref.: #60903]

Incubation period

3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16740]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #16740]

Culture medium growth

[Ref.: #16740]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16740]

Culture medium composition

expand / minimize

[Ref.: #40639]

Culture medium

MEDIUM 6 - Columbia agar with 10 % horse blood

[Ref.: #40639]

Culture medium growth

[Ref.: #40639]

Culture medium composition

expand / minimize

[Ref.: #118817]

Culture medium

CIP Medium 45

[Ref.: #118817]

Culture medium growth

[Ref.: #118817]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #118817]

Culture medium

CIP Medium 6

[Ref.: #118817]

Culture medium growth

[Ref.: #118817]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #16740]	growth	37 °C
[Ref.: #29146]	growth	37-42 °C
[Ref.: #29146]	optimum	39.5 °C
[Ref.: #40639]	growth	37 °C
[Ref.: #60903]	growth	37 °C

[Ref.: #16740]	Temperature range	mesophilic
[Ref.: #29146]	Temperature range	mesophilic
[Ref.: #40639]	Temperature range	mesophilic
[Ref.: #60903]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29146]			optimum	4

Information on physiology and metabolism Physiology and metabolism

[Ref.: #16740]	Oxygen tolerance	microaerophile
[Ref.: #29146]	Oxygen tolerance	microaerophile
[Ref.: #60903]	Oxygen tolerance	microaerophile
[Ref.: #118817]	Oxygen tolerance	microaerophile

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.992

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #29146]		NaCl		optimum	1	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #29146]	acetate ChEBI	+	carbon source
[Ref.: #68373]	acetate ChEBI	-	assimilation	* from API CAM
[Ref.: #68373]	citrate ChEBI	-	assimilation	* from API CAM
[Ref.: #68373]	D-glucose ChEBI	-	assimilation	* from API CAM
[Ref.: #68373]	hippurate ChEBI	-	hydrolysis	* from API CAM
[Ref.: #68373]	malate ChEBI	-	assimilation	* from API CAM
[Ref.: #29146]	nitrate ChEBI	+	reduction
[Ref.: #118817]	nitrate ChEBI	+	reduction
[Ref.: #118817]	nitrite ChEBI	-	reduction
[Ref.: #68373]	triphenyltetrazolium chloride ChEBI	+	reduction	* from API CAM
[Ref.: #68373]	urea ChEBI	-	hydrolysis	* from API CAM
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #68373]		erythromycin ChEBI	yes	14	µg	* from API CAM

	Metabolite production	Metabolite	Production
[Ref.: #118817]		indole ChEBI	no
[Ref.: #68373]		hydrogen sulfide ChEBI	no	* from API CAM

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #16740]	catalase	+	1.11.1.6
[Ref.: #29146]	catalase	+	1.11.1.6
[Ref.: #118817]	catalase	+	1.11.1.6
[Ref.: #68373]	catalase	+	1.11.1.6	* from API CAM
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #29146]	cytochrome oxidase	+	1.9.3.1
[Ref.: #16740]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #68373]	esterase	+		* from API CAM
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68373]	L-aspartate arylamidase	-	3.4.11.21	* from API CAM
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #118817]	oxidase	+
[Ref.: #68373]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API CAM
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #118817]	urease	-	3.5.1.5
[Ref.: #68373]	urease	-	3.5.1.5	* from API CAM
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® CAM:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Reduction of nitrateNIT	EsteraseEST	Hippuric acidHIP	gamma-Glutamyl transferaseGGT	Reduction of Triphenyl tetrazolium chlorideTTC	Pyrrolidonyl arylamidasePYRA	L-arginine arylamidaseArgA	L-aspartic acid arylamidaseAspA	Alkaline phosphatasePAL	H2S productionH2S	Assimilation of D-glucoseGLU	Assimilation of succinateSUT	Growth inhibitionNAL	Growth inhibitionCFZ	Assimilation of acetateACE	Assimilation of propionic acidPROP	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Erythromycin resistance (+) sensitivity (-)ERO	CatalaseCAT
[Ref.: #16740]	3794	-	+	+	-	+/-	+	-	+	-	+	-	-	+	+	+	-	+	-	-	-	+
[Ref.: #16740]	3793	-	-	+	-	-	+	-	+/-	-	+/-	-	-	-	-	-	-	-	-	-	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #118817]	12295	-	-	+	-	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16740]	Sample type/isolated from	rabbit caecal contents Oryctolagus cuniculus
[Ref.: #16740]	Host species	Oryctolagus cuniculus

[Ref.: #60903]	Sample type/isolated from	Rabbit caecal contents
[Ref.: #60903]	Sampling date	2005-03-01
[Ref.: #60903]	Geographic location (country and/or sea, region)	Emilia Romagna region
[Ref.: #60903]	Country	Italy
[Ref.: #60903]	Country ISO 3 Code	ITA
[Ref.: #60903]	Continent	Europe

[Ref.: #118817]	Sample type/isolated from	Animal, Rabbit, caecal contents
[Ref.: #118817]	Geographic location (country and/or sea, region)	Emilia Romagna region
[Ref.: #118817]	Country	Italy
[Ref.: #118817]	Country ISO 3 Code	ITA
[Ref.: #118817]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Leporidae (Rabbit/Hare)
#Host Body Product	#Gastrointestinal tract	#Caecal content

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence DQ400345 (>99% sequence identity) for Campylobacter cuniculorum subclade from Microbeatlas

Aquatic Samples	102
Soil Samples	16
Animal Samples	2901
Plant Samples	4
Total Samples	3023

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16740]	Pathogenicity (animal)	yes, in single cases
[Ref.: #16740]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #118817]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16740]	Campylobacter cuniculorum strain 150B 16S ribosomal RNA gene, partial sequence	DQ400345	1390	ENA	1121267 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Campylobacter cuniculorum LMG 24588	2773857804	complete	JGI IMG/M	1121267 tax ID	*
[Ref.: #66792]	Campylobacter cuniculorum DSM 23162 = LMG 24588	GCA_000621005	scaffold	GenBank	1121267 tax ID	*
[Ref.: #66792]	Campylobacter cuniculorum DSM 23162	1121267.3	wgs	PATRIC	1121267 tax ID	*
[Ref.: #66792]	Campylobacter cuniculorum DSM 23162 = LMG 24588	1121267.6	plasmid	PATRIC	1121267 tax ID	*
[Ref.: #66792]	Campylobacter cuniculorum DSM 23162 = LMG 24588	1121267.7	plasmid	PATRIC	1121267 tax ID	*
[Ref.: #66792]	Campylobacter cuniculorum LMG 24588 strain LMG 24588	1031742.3	complete	PATRIC	1121267 tax ID	*

[Ref.: #16740]	GC-content	32.4 mol%	high performance liquid chromatography (HPLC)
[Ref.: #29146]	GC-content	32.4 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Campylobacter cuniculorum DSM 23162 = LMG 24588 LMG 24588 NCBI: GCA_002104335.1
[Ref.: #69481]	spore-forming	no	53	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Campylobacter cuniculorum DSM 23162 = LMG 24588 LMG 24588 NCBI: GCA_002104335.1
[Ref.: #69480]	flagellated	yes	62.655	no
[Ref.: #69480]	gram-positive	no	98.584	no
[Ref.: #69480]	anaerobic	no	91.546	yes
[Ref.: #69480]	aerobic	no	93.305	yes
[Ref.: #69480]	halophile	no	91.311	yes
[Ref.: #69480]	spore-forming	no	94.582	no
[Ref.: #69480]	glucose-util	no	87.367	yes
[Ref.: #69480]	thermophile	no	73.2	yes
[Ref.: #69480]	motile	yes	89.368	no
[Ref.: #69480]	glucose-ferment	no	88.665	no

Availability in culture collections External links

[Ref.: #16740]

Culture collection no.

CIP 110020, DSM 23162, CCUG 56289, LMG 24588

[Ref.: #71768]

SI-ID 292326

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete Genome Sequence of the Campylobacter cuniculorum Type Strain LMG 24588.	Miller WG, Yee E, Revez J, Bono JL, Rossi M	Genome Announc	10.1128/genomeA.00543-17	2017	*
Phylogeny	Campylobacter cuniculorum sp. nov., from rabbits.	Zanoni RG, Debruyne L, Rossi M, Revez J, Vandamme P	Int J Syst Evol Microbiol	10.1099/ijs.0.007286-0	2009	*

References

#16740

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23162

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29146

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25570 (see below)

#40639 ; Curators of the CIP;
#60903 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 56289

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68373

Automatically annotated from API CAM .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#71768

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID292326.1 )

#118817 Collection of Institut Pasteur ; Curators of the CIP; CIP 110020

#25570

IJSEM 1666 2009 ( DOI 10.1099/ijs.0.007286-0 , PubMed 19542108 )

* These data were automatically processed and therefore are not curated

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Campylobacter cuniculorum DSM 23162 = LMG 24588 LMG 24588

Campylobacter cuniculorum DSM 23162 = LMG 24588 LMG 24588

Change proposal

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