Name: Ralstonia pickettii K-288
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2085

Type strain:

Species: Ralstonia pickettii

Strain Designation: K-288

Culture col. no.: DSM 6297, ATCC 27511, CIP 73.23, JCM 5969, NCTC 11149, CCUG 3318, LMG 5942, BCRC 14820, CECT 330, CGMCC 1.1807, IAM 13530, ICPB 3981, NBRC 102503, NCIMB 13142, VTT E-981068

Strain history: Y. Kosako 85011 <-- Nissui Pharm. Co., Ltd. <-- ATCC 27511 <-- N. J. Palleroni <-- M. Pickett K-288.

NCBI tax ID(s): 1218114 (strain), 329 (species)

Other strains from Ralstonia pickettii

Section

Ralstonia pickettii K-288 is an aerobe, thermophilic, rod-shaped bacterium of the family Burkholderiaceae.

rod-shaped
aerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Burkholderiaceae*
	Genus	*Ralstonia*
	Species	**Ralstonia pickettii**
	Full Scientific Name (LPSN)	Ralstonia pickettii (Ralston et al. 1973) Yabuuchi et al. 1996

	Synonym	Pseudomonas pickettii
	Synonym	Burkholderia pickettii

Information on morphological and physiological properties Morphology

[Ref.: #22944]	Cell shape	rod-shaped
[Ref.: #22944]	Flagellum arrangement	monotrichous, polar

Information on culture and growth conditions Culture and growth conditions

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Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #2581]

Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #2581]

Culture medium composition

expand / minimize

[Ref.: #2581]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #2581]

Culture medium growth

[Ref.: #2581]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2581]

Culture medium composition

expand / minimize

[Ref.: #36535]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #36535]

Culture medium growth

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Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #2581]		growth	30 °C
[Ref.: #22943]	inconsistent	growth	42 °C
[Ref.: #22944]	inconsistent	growth	42 °C
[Ref.: #36535]		growth	30 °C
[Ref.: #44638]		growth	30-37 °C
[Ref.: #67770]		growth	30 °C

[Ref.: #2581]	Temperature range	mesophilic
[Ref.: #22943]	Temperature range	thermophilic
[Ref.: #22944]	Temperature range	thermophilic
[Ref.: #36535]	Temperature range	mesophilic
[Ref.: #44638]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #44638]

Oxygen tolerance

aerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #22944]	3-hydroxybenzoate ChEBI	-	growth
[Ref.: #22944]	acetate ChEBI	+	builds base from
[Ref.: #22943]	adipate ChEBI	+/-	growth
[Ref.: #22944]	allantoin ChEBI	-	builds base from
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #22943]	citrate ChEBI	+	growth
[Ref.: #22944]	citrate ChEBI	+	builds base from
[Ref.: #22944]	D-arabitol ChEBI	-	builds acid from
[Ref.: #22943]	D-fructose ChEBI	+/-	fermentation
[Ref.: #22943]	D-glucose ChEBI	+	growth
[Ref.: #22943]	D-glucose ChEBI	+/-	fermentation
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #22943]	D-xylose ChEBI	+/-	fermentation
[Ref.: #22944]	D-xylose ChEBI	+	builds acid from
[Ref.: #22943]	decanoate ChEBI	+/-	growth
[Ref.: #22944]	decanoate ChEBI	+	growth
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #22944]	galactarate ChEBI	+	builds base from
[Ref.: #22944]	galacturonate ChEBI	+	builds base from
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #22944]	glucose ChEBI	+	builds acid from
[Ref.: #22943]	L-arabinose ChEBI	+	growth
[Ref.: #22944]	L-arabinose ChEBI	+	builds acid from
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #22944]	lactate ChEBI	+	builds base from
[Ref.: #22944]	lactose ChEBI	-	builds acid from
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #22944]	maleic acid ChEBI	+	builds base from
[Ref.: #22944]	malonate ChEBI	+	builds base from
[Ref.: #22944]	maltose ChEBI	-	builds acid from
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #22944]	mannitol ChEBI	-	builds acid from
[Ref.: #22944]	myo-inositol ChEBI	-	builds acid from
[Ref.: #22943]	N-acetyl-D-glucosamine ChEBI	+	growth
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #22943]	nitrate ChEBI	+	reduction
[Ref.: #22944]	nitrate ChEBI	+	reduction
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #22944]	oxalate ChEBI	-	builds base from
[Ref.: #22943]	phenylacetate ChEBI	+/-	growth
[Ref.: #22944]	propionate ChEBI	+	growth
[Ref.: #22944]	suberate ChEBI	+	growth
[Ref.: #22944]	sucrose ChEBI	-	builds acid from
[Ref.: #22944]	tartrate ChEBI	+	builds base from
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #22943]	tween 80 ChEBI	+	growth
[Ref.: #22944]	tween 80 ChEBI	+	hydrolysis
[Ref.: #22943]	urea ChEBI	+/-	hydrolysis
[Ref.: #22944]	urea ChEBI	+	hydrolysis
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic intermediate	Concentration
[Ref.: #22943]		penicillin ChEBI	yes	10	µg (disc)

	Metabolite production	Metabolite
[Ref.: #68369]		indole ChEBI	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #22944]	alkaline phosphatase	-	3.1.3.1
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #22943]	catalase	-	1.11.1.6
[Ref.: #22944]	catalase	+	1.11.1.6
[Ref.: #22943]	cystine arylamidase	-	3.4.11.3
[Ref.: #22943]	cytochrome oxidase	-	1.9.3.1
[Ref.: #22944]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #22943]	esterase (C 4)	+/-
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #22943]	lipase (C 14)	+
[Ref.: #22944]	phenylalanine deaminase	+	4.3.1.5
[Ref.: #22943]	phosphoamidase	+/-	3.9.1.1
[Ref.: #22944]	pyrrolidonyl arylamidase	+	3.4.19.3
[Ref.: #22943]	urease	+/-	3.5.1.5
[Ref.: #22944]	urease	+	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #2581]	22167	+	-	-	-	-	-	-	-	+	+	-	-	-	-	+	+	+/-	+	+	+/-	+
[Ref.: #2581]	20744	+	-	-	-	-	-	-	-	+	+	-	-	-	-	+	+	-	+	+	-	+
[Ref.: #2581]	20724	+	-	-	-	-	-	-	-	+	+	-	-	-	-	+	+	-	+	+	+	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #44638]	Sample type/isolated from	Human,patient who had undergone tracheotomy
[Ref.: #44638]	Country	USA
[Ref.: #44638]	Country ISO 3 Code	USA
[Ref.: #44638]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Patient who had undergone tracheotomy

[Ref.: #36535]	Country	USA
[Ref.: #36535]	Country ISO 3 Code	USA
[Ref.: #36535]	Continent	North America
* marker position based on {}

Isolation sources categories

#Infection	#Medical environment	#Medical practice
#Host Body-Site	#Oral cavity and airways	#Trachea
#Host	#Human	-
#Infection	#Patient	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LN681565 (>99% sequence identity) for Ralstonia pickettii from Microbeatlas

Aquatic Samples	1121
Soil Samples	338
Animal Samples	4145
Plant Samples	2723
Total Samples	8327

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2581]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #2581]	Ralstonia pickettii partial 16S rRNA gene, type strain DSM 6297T	LN681565	1490	ENA	329 tax ID
[Ref.: #20218]	Ralstonia pickettii 16S ribosomal RNA gene, partial sequence	AF467977	995	ENA	329 tax ID	*
[Ref.: #20218]	Ralstonia pickettii strain ATCC 27511 16S ribosomal RNA gene, partial sequence	AY741342	1491	ENA	329 tax ID	*
[Ref.: #20218]	Ralstonia pickettii gene for 16S rRNA, partial sequence, strain: ATCC 27511 (= EY 1917)	D13047	158	ENA	329 tax ID	*
[Ref.: #20218]	Pseudomonas pickettii 16S ribosomal RNA (16S rRNA) gene, transfer RNA-Ile (tRNA-Ile) gene, transfer RNA-Ala (tRNA-Ala) gene, 23S ribosomal RNA (23S rRNA) gene	L28163	704	ENA	329 tax ID	*
[Ref.: #20218]	16S rRNA [Burkholderia pickettii, ATCC 27511, Genomic, 970 nt, segment 1 of 2]	S55004	970	ENA	329 tax ID	*
[Ref.: #20218]	16S rRNA [Burkholderia pickettii, ATCC 27511, Genomic, 377 nt, segment 2 of 2]	S55018	377	ENA	329 tax ID	*
[Ref.: #20218]	Ralstonia pickettii strain BCRC 14820 16S-23S ribosomal RNA intergenic spacer, complete sequence	EU014524	515	ENA	329 tax ID	*
[Ref.: #20218]	Ralstonia pickettii strain CIP 7323 16S ribosomal RNA gene, partial sequence	EU024148	1316	ENA	329 tax ID	*
[Ref.: #20218]	Ralstonia pickettii gene for 16S rRNA, partial sequence, strain: JCM 5969	AB626131	1460	ENA	329 tax ID	*
[Ref.: #20218]	Ralstonia pickettii 16S rRNA gene, ITS1 and 23S rRNA gene, strain JCM5969	AM501933	827	ENA	329 tax ID	*
[Ref.: #20218]	Ralstonia pickettii 16S rRNA gene, 23S rRNA gene and ITS1, specimen voucher LMG5942	AJ783972	888	ENA	329 tax ID	*
[Ref.: #20218]	Ralstonia pickettii gene for 16S rRNA, partial sequence, strain: NBRC 102503	AB681837	1463	ENA	329 tax ID	*
[Ref.: #20218]	P.pickettii (group 2) 16S rRNA subunit	X70349	294	ENA	329 tax ID	*
[Ref.: #20218]	Ralstonia pickettii partial 16S rRNA gene, strain PSE055, ATCC 27512	AJ270265	474	ENA	329 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Ralstonia pickettii NBRC 102503 NBRC 102503	GCA_001544155	contig	GenBank	1218114 tax ID
[Ref.: #67770]	Ralstonia pickettii ATCC 27511	GCA_000743455	scaffold	GenBank	329 tax ID
[Ref.: #67770]	Ralstonia pickettii NCTC11149	GCA_900455835	contig	GenBank	329 tax ID
[Ref.: #66792]	Ralstonia pickettii strain ATCC 27511	329.18	complete	PATRIC	329 tax ID	*
[Ref.: #66792]	Ralstonia pickettii strain FDAARGOS_1535 strain Not applicable	329.188	complete	PATRIC	329 tax ID	*
[Ref.: #66792]	Ralstonia pickettii NBRC 102503	2731957562	draft	JGI IMG/M	1218114 tax ID	*

[Ref.: #22943]

GC-content

64 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #2581]

Culture collection no.

DSM 6297, ATCC 27511, CIP 73.23, JCM 5969, NCTC 11149, CCUG 3318, LMG 5942, BCRC 14820, CECT 330, CGMCC 1.1807, IAM 13530, ICPB 3981, NBRC 102503, NCIMB 13142, VTT E-981068

Literature:

	Title	Authors	Journal	DOI	Year
Genetics	Structure of the O-specific polysaccharide from Burkholderia pickettii strain NCTC 11149.	Galbraith L, George R, Wyklicky J, Wilkinson SG	Carbohydr Res	10.1016/0008-6215(95)00387-8	1996	*
Phylogeny	Transfer of two Burkholderia and an Alcaligenes species to Ralstonia gen. Nov.: Proposal of Ralstonia pickettii (Ralston, Palleroni and Doudoroff 1973) comb. Nov., Ralstonia solanacearum (Smith 1896) comb. Nov. and Ralstonia eutropha (Davis 1969) comb. Nov.	Yabuuchi E, Kosako Y, Yano I, Hotta H, Nishiuchi Y	Microbiol Immunol	10.1111/j.1348-0421.1995.tb03275.x	1995	*
	Draft Genome Assembly of Ralstonia pickettii Type Strain K-288 (ATCC 27853).	Daligault HE, Davenport KW, Minogue TD, Broomall SM, Bruce DC, Chain PS, Coyne SR, Gibbons HS, Jaissle J, Lo CC, Meincke L, Munk AC, Rosenzweig CN, Johnson SL	Genome Announc	10.1128/genomeA.00973-14	2014	*

References

#2581

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 6297

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#22943

P. Vandamme, J. Goris, T. Coenye, B. Hoste, D. Janssens, K. Kersters, P. De Vos, E. Falsen: Assignment of Centers for Disease Control group IVc-2 to the genus Ralstonia as Ralstonia paucula sp. nov.. IJSEM 49: 663 - 669 1999 ( DOI 10.1099/00207713-49-2-663 , PubMed 10319489 )

#22944

T De Baere, S Steyaert, G Wauters, P Des Vos, J Goris, T Coenye, T Suyama, G Verschraegen, M Vaneechoutte: Classification of Ralstonia pickettii biovar 3/'thomasii' strains (Pickett 1994) and of new isolates related to nosocomial recurrent meningitis as Ralstonia mannitolytica sp. nov.. IJSEM 51: 547 - 558 2001 ( DOI 10.1099/00207713-51-2-547 , PubMed 11321101 )

#36535 ; Curators of the CIP;
#44638 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 3318

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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