Ralstonia pickettii (DSM 6297, ATCC 27511, CIP 73.23)

Name: Ralstonia pickettii (DSM 6297, ATCC 27511, CIP 73.23)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2085

Type strain

Strain Designation K-288, K 288

Culture col. no. DSM 6297 ATCC 27511 CIP 73.23 JCM 5969 NCTC 11149 12 more

NCBI tax ID(s) 1218114 329

Special Collections DSMZ CCUG JCM CIP Literature strains

History DSMZ <- CIP; CIP 73.23 <- F. Pichinoty; <- M. J. Picketti; K-288 JCM <-- Y. Kosako 85011 <-- Nissui Pharm. Co., Ltd. <-- ATCC 27511 <-- N. J. Palleroni <-- M. Pickett K-288. CIP <- 1973, F. Pichinoty, CNRS, Marseille, France, Pseudomonas pickettii <- M.J. Pickett, California, USA: strain K 288

Links

Ralstonia pickettii K-288 is an aerobe, rod-shaped bacterium of the family Burkholderiaceae.

rod-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 4172

LMG 5942,ATCC 27511,CCUG 3318,JCM 5969,NCTC 11149,CCRC 14820,IAM 13530,NCIMB 13142,CECT 330,CFBP 2459,AS 1.1807,NCCB 92016,LMD 92.16,BCRC 14820,HAMBI 2158,KACC 10472,CIP 73.23,NBRC 102503,CNCTC 5826,CGMCC 1.1759,CGMCC 1.1807,VTT E-981068,DSM 6297

@ref 20215

Last LPSN update 2026-05-29 08:25:24

Domain Bacteria

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Burkholderiaceae

Genus Ralstonia

Species Ralstonia pickettii

Full scientific name Ralstonia pickettii (Ralston et al. 1973) Yabuuchi et al. 1996

Synonyms (2)

Pseudomonas pickettii
Burkholderia pickettii

BacDive ID	Other strains from **Ralstonia pickettii** (63)	Type strain
134624	R. pickettii Jammet, CIP 74.22, LMG 7160
134834	R. pickettii K 300, CIP 73.24, LMG 7159, CCUG 38410
134906	R. pickettii CIP 102737
136115	R. pickettii 605-86, CIP 102614
138027	R. pickettii 524.86, CIP 102543
138706	R. pickettii CIP 102242
138749	R. pickettii 752.86, CIP 102735
141404	R. pickettii CCUG 1467
141427	R. pickettii CCUG 1742
141479	R. pickettii CCUG 2165, LMG 7008
141579	R. pickettii CCUG 3314
141580	R. pickettii CCUG 3316
141581	R. pickettii CCUG 3317, LMG 7003
141582	R. pickettii CCUG 3319, LMG 7004
141585	R. pickettii CCUG 3324
141588	R. pickettii CCUG 3339
141942	R. pickettii CCUG 8527
142020	R. pickettii CCUG 9984
142063	R. pickettii CCUG 10219
142314	R. pickettii CCUG 11629, LMG 7011
142399	R. pickettii CCUG 12327
142411	R. pickettii CCUG 12413
142433	R. pickettii CCUG 12490
142434	R. pickettii CCUG 12491, LMG 7014
142435	R. pickettii CCUG 12492, LMG 7015
142436	R. pickettii CCUG 12493
142437	R. pickettii CCUG 12494
142492	R. pickettii CCUG 12664 B
142612	R. pickettii CCUG 13311
142688	R. pickettii CCUG 13925
142866	R. pickettii CCUG 15418
142871	R. pickettii CCUG 15447
142872	R. pickettii CCUG 15448
142889	R. pickettii CCUG 15607
143031	R. pickettii CCUG 16426
143155	R. pickettii CCUG 17515
143304	R. pickettii CCUG 18336
143390	R. pickettii CCUG 18841, CCM 2846, LMG 6871
143662	R. pickettii CCUG 20865
143695	R. pickettii CCUG 21039
145004	R. pickettii CCUG 27371
145460	R. pickettii CCUG 28597
145693	R. pickettii CCUG 29272
146323	R. pickettii CCUG 30895
147997	R. pickettii CCUG 34642
149008	R. pickettii CCUG 36717
149792	R. pickettii CCUG 38409, LMG 7140
149953	R. pickettii CCUG 38749, LMG 18088
149961	R. pickettii CCUG 38760, LMG 18092
149962	R. pickettii CCUG 38761, LMG 18093
149963	R. pickettii CCUG 38762, LMG 18094
149964	R. pickettii CCUG 38763, LMG 18095
149965	R. pickettii CCUG 38764, LMG 18096
150487	R. pickettii CCUG 41417
150574	R. pickettii CCUG 41639
150782	R. pickettii CCUG 42308 B
151359	R. pickettii CCUG 43847
152062	R. pickettii CCUG 45668
153704	R. pickettii CCUG 50693
155402	R. pickettii CCUG 57614
156558	R. pickettii CCUG 62660
156734	R. pickettii CCUG 64635
174763	R. pickettii Dres_RalPick_01, DSM 105835

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Flagellum arrangement	Confidence
22944		rod-shaped	monotrichous, polar
121820	negative	rod-shaped
125438	negative			97.5
125439	negative			98.119

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
2581	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
36535	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
121820	CIP Medium 72	Medium recipe at CIP
2581	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)	Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
2581	positive	growth	30
22943	inconsistent	growth	42
22944	inconsistent	growth	42
36535	positive	growth	30
44638	positive	growth	30-37
67770	positive	growth	30
121820	positive	growth	30-41
121820	negative	growth	5
121820	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
44638	aerobe
121820	obligate aerobe
125439	obligate aerobe	99.656

Spore formation

@ref	Spore formation	Confidence
125439		99.285

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
22944	16193 ChEBI	3-hydroxybenzoate	-	growth
22944	30089 ChEBI	acetate	+	builds base from
22943	17128 ChEBI	adipate	+/-	growth
22944	15676 ChEBI	allantoin	-	builds base from
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
22943	16947 ChEBI	citrate	+	growth
22944	16947 ChEBI	citrate	+	builds base from
121820	16947 ChEBI	citrate	+	carbon source
22944	18333 ChEBI	D-arabitol	-	builds acid from
22943	15824 ChEBI	D-fructose	+/-	fermentation
22943	17634 ChEBI	D-glucose	+	growth
22943	17634 ChEBI	D-glucose	+/-	fermentation
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
22943	65327 ChEBI	D-xylose	+/-	fermentation
22944	65327 ChEBI	D-xylose	+	builds acid from
22943	27689 ChEBI	decanoate	+/-	growth
22944	27689 ChEBI	decanoate	+	growth
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
121820	4853 ChEBI	esculin	-	hydrolysis
22944	16537 ChEBI	galactarate	+	builds base from
22944	24175 ChEBI	galacturonate	+	builds base from
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
22944	17234 ChEBI	glucose	+	builds acid from
22943	30849 ChEBI	L-arabinose	+	growth
22944	30849 ChEBI	L-arabinose	+	builds acid from
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
22944	24996 ChEBI	lactate	+	builds base from
22944	17716 ChEBI	lactose	-	builds acid from
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
22944	18300 ChEBI	maleic acid	+	builds base from
22944	15792 ChEBI	malonate	+	builds base from
22944	17306 ChEBI	maltose	-	builds acid from
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
22944	29864 ChEBI	mannitol	-	builds acid from
22944	17268 ChEBI	myo-inositol	-	builds acid from
22943	506227 ChEBI	N-acetyl-D-glucosamine	+	growth
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
22943	17632 ChEBI	nitrate	+	reduction
22944	17632 ChEBI	nitrate	+	reduction
121820	17632 ChEBI	nitrate	+	reduction
121820	17632 ChEBI	nitrate	+	respiration
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
121820	16301 ChEBI	nitrite	+	reduction
22944	30623 ChEBI	oxalate	-	builds base from
121820	15882 ChEBI	phenol	+	degradation
22943	18401 ChEBI	phenylacetate	+/-	growth
22944	17272 ChEBI	propionate	+	growth
22944	76282 ChEBI	suberate	+	growth
22944	17992 ChEBI	sucrose	-	builds acid from
22944	132950 ChEBI	tartrate	+	builds base from
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
22943	53426 ChEBI	tween 80	+	growth
22944	53426 ChEBI	tween 80	+	hydrolysis
22943	16199 ChEBI	urea	+/-	hydrolysis
22944	16199 ChEBI	urea	+	hydrolysis
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Antibiotic resistance

@ref	ChEBI	Group ID	Metabolite	Is intermediate	Intermediate conc.
22943	17334	0	penicillin		10 µg (disc)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE
121820	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test	Voges-proskauer-test
121820	15688 ChEBI	acetoin		-
68369	35581 ChEBI	indole	-		from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
121820	alcohol dehydrogenase	-	1.1.1.1
22944	alkaline phosphatase	-	3.1.3.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
121820	amylase	+
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
22943	catalase	-	1.11.1.6
22944	catalase	+	1.11.1.6
121820	catalase	-	1.11.1.6
22943	cystine arylamidase	-	3.4.11.3
68382	cystine arylamidase	+	3.4.11.3	from API zym
22943	cytochrome oxidase	-	1.9.3.1
22944	cytochrome oxidase	+	1.9.3.1
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
121820	DNase	-
22943	esterase (C 4)	+/-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
121820	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
121820	lipase	+
22943	lipase (C 14)	+
68382	lipase (C 14)	+		from API zym
121820	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
121820	ornithine decarboxylase	-	4.1.1.17
121820	oxidase	+
121820	phenylalanine ammonia-lyase	-	4.3.1.24
22944	phenylalanine deaminase	+	4.3.1.5
22943	phosphoamidase	+/-	3.9.1.1
22944	pyrrolidonyl arylamidase	+	3.4.19.3
68382	trypsin	-	3.4.21.4	from API zym
121820	tryptophan deaminase	-
22943	urease	+/-	3.5.1.5
22944	urease	+	3.5.1.5
121820	urease	-	3.5.1.5
68369	urease	-	3.5.1.5	from API 20NE
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
121820	-	+	+	+	+	+	-	+	-	-	+	+	-	-	-	-	-	-	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
2581	+	-	-	-	-	-	-	-	+	+	-	-	-	-	+	+	+/-	+	+	+/-	+
2581	+	-	-	-	-	-	-	-	+	+	-	-	-	-	+	+	-	+	+	-	+
2581	+	-	-	-	-	-	-	-	+	+	-	-	-	-	+	+	-	+	+	+	+
2581	+	-	-	-	-	+/-	+	-	+	+	-	-	-	-	+	+	+/-	+	+	-	+

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
121820	not determinedn.d.	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	+	+	+	+	-	+	+	+	+	-	+	-	+	-	+	+	+	-	-	-	-	-	-	-	-	+	-	+	+	-	-	+	+	+	+	-	-	-	+	-	+	+	+	+	+	+	+	+	+	+	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Infection	#Medical environment	#Medical practice
#Host Body-Site	#Oral cavity and airways	#Trachea
#Host	#Human	-
#Infection	#Patient	-

Isolation

@ref	Sample type	Host species	Country	Country ISO 3 Code	Continent	Isolation date
36535			USA	USA	North America
44638	Human,patient who had undergone tracheotomy	Homo sapiens	USA	USA	North America
67770	Patient who had undergone tracheotomy	Homo sapiens
121820	Patient who had undergone tracheotomy	Homo sapiens	United States of America	USA	North America	1972

Taxonmaps

69479

Global distribution of 16S sequence LN681565 (>99% sequence identity) for Ralstonia pickettii from Microbeatlas

Aquatic Samples	1121
Soil Samples	338
Animal Samples	4145
Plant Samples	2723
Total Samples	8327

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
2581	2	Risk group (German classification) According to TRBA 466
121820	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	Ralstonia pickettii strain FDAARGOS_1535	complete		329.189		329	98.76
66792	Ralstonia pickettii strain FDAARGOS_1535	complete		329.188		329	98.76
66792	ASM1646641v2 assembly for Ralstonia pickettii K-288	complete	GCA_016466415	329.112	8113792790	329	94.48
124043	ASM2034145v1 assembly for Ralstonia pickettii FDAARGOS_1535	chromosome	GCA_020341455	329.187		329	82.32
67770	51699_E01 assembly for Ralstonia pickettii NCTC11149	contig	GCA_900455835	329.39	2859196362	329	73.4
67770	RPT assembly for Ralstonia pickettii ATCC 27511	scaffold	GCA_000743455	329.18	2597490112	329	69.56
67770	ASM154415v1 assembly for Ralstonia pickettii NBRC 102503	contig	GCA_001544155	1218114.3	2731957562	1218114	64.59

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Ralstonia pickettii 16S ribosomal RNA gene, partial sequence	AF467977	995	329
20218	Ralstonia pickettii strain ATCC 27511 16S ribosomal RNA gene, partial sequence	AY741342	1491	329
20218	Ralstonia pickettii gene for 16S rRNA, partial sequence, strain: ATCC 27511 (= EY 1917)	D13047	158	329
20218	Pseudomonas pickettii 16S ribosomal RNA (16S rRNA) gene, transfer RNA-Ile (tRNA-Ile) gene, transfer RNA-Ala (tRNA-Ala) gene, 23S ribosomal RNA (23S rRNA) gene	L28163	704	329
20218	Ralstonia pickettii 16S rRNA gene, complete sequence	AH004177	970	329
20218	Ralstonia pickettii strain BCRC 14820 16S-23S ribosomal RNA intergenic spacer, complete sequence	EU014524	515	329
20218	Ralstonia pickettii strain CIP 7323 16S ribosomal RNA gene, partial sequence	EU024148	1316	329
20218	Ralstonia pickettii gene for 16S rRNA, partial sequence, strain: JCM 5969	AB626131	1460	329
20218	Ralstonia pickettii 16S rRNA gene, ITS1 and 23S rRNA gene, strain JCM5969	AM501933	827	329
20218	Ralstonia pickettii 16S rRNA gene, 23S rRNA gene and ITS1, specimen voucher LMG5942	AJ783972	888	329
20218	Ralstonia pickettii gene for 16S rRNA, partial sequence, strain: NBRC 102503	AB681837	1463	329
20218	P.pickettii (group 2) 16S rRNA subunit	X70349	294	329
20218	Ralstonia pickettii partial 16S rRNA gene, strain PSE055, ATCC 27512	AJ270265	474	329
2581	Ralstonia pickettii partial 16S rRNA gene, type strain DSM 6297T	LN681565	1490	329
124043	Ralstonia pickettii strain ATCC 27511 16S ribosomal RNA gene, partial sequence.	OM818507	1526	329

GC content

@ref	GC-content (mol%)	Method
22943	64	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Ralstonia pickettii strain FDAARGOS_1535
BV-BRC: 329.188

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.66	no
125439	gram_stain	BacteriaNetⓘ	negative	98.12	no
125439	motility	BacteriaNetⓘ	yes	71.91	no
125439	spore_formation	BacteriaNetⓘ	no	99.29	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Ralstonia pickettii K-288
NCBI: GCA_016466415.2

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	97.50	no
125438	anaerobic	anaerobicⓘ	no	97.13	no
125438	aerobic	aerobicⓘ	yes	86.79	yes
125438	spore-forming	spore-formingⓘ	no	87.46	no
125438	thermophilic	thermophileⓘ	no	98.00	yes
125438	flagellated	motile2+ⓘ	yes	82.35	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Ultrasound-assisted extraction and flavor quality assessment of in vitro biomimetically fermented Kopi Luwak.	Duan S, Qiao Z, Chen Y, Shen Y, Du Z, Dong J, Yu L, Li Y, Yang R, Fang C.	Ultrason Sonochem	10.1016/j.ultsonch.2025.107499	2025
	Stereospecific control of microbial growth by a combinatoric suite of chiral siderophores.	Stow PR, Forsch KO, Thomsen E, Naka H, Haygood MG, Barbeau KA, Butler A.	Proc Natl Acad Sci U S A	10.1073/pnas.2423730122	2025
	Intestinal Ralstonia pickettii augments glucose intolerance in obesity.	Udayappan SD, Kovatcheva-Datchary P, Bakker GJ, Havik SR, Herrema H, Cani PD, Bouter KE, Belzer C, Witjes JJ, Vrieze A, de Sonnaville ESV, Chaplin A, van Raalte DH, Aalvink S, Dallinga-Thie GM, Heilig HGHJ, Bergstrom G, van der Meij S, van Wagensveld BA, Hoekstra JBL, Holleman F, Stroes ESG, Groen AK, Backhed F, de Vos WM, Nieuwdorp M.	PLoS One	10.1371/journal.pone.0181693	2017
Enzymology	Genotypic and phenotypic diversity of Ralstonia pickettii and Ralstonia insidiosa isolates from clinical and environmental sources including High-purity Water. Diversity in Ralstonia pickettii.	Ryan MP, Pembroke JT, Adley CC.	BMC Microbiol	10.1186/1471-2180-11-194	2011
Enzymology	Comprehensive Laboratory Evaluation of a Specific Lateral Flow Assay for the Presumptive Identification of Francisella tularensis in Suspicious White Powders and Aerosol Samples.	Pillai SP, DePalma L, Prentice KW, Ramage JG, Chapman C, Sarwar J, Parameswaran N, Petersen J, Yockey B, Young J, Singh A, Pillai CA, Manickam G, Thirunavkkarasu N, Avila JR, Sharma S, Morse SA, Venkateswaran K, Anderson K, Hodge DR.	Health Secur	10.1089/hs.2019.0151	2020
Enzymology	Comprehensive Laboratory Evaluation of a Lateral Flow Assay for the Detection of Yersinia pestis.	Prentice KW, DePalma L, Ramage JG, Sarwar J, Parameswaran N, Petersen J, Yockey B, Young J, Joshi M, Thirunavvukarasu N, Singh A, Chapman C, Avila JR, Pillai CA, Manickam G, Sharma SK, Morse SA, Venkateswaran KV, Anderson K, Hodge DR, Pillai SP.	Health Secur	10.1089/hs.2019.0094	2019
Enzymology	Comprehensive Laboratory Evaluation of a Highly Specific Lateral Flow Assay for the Presumptive Identification of Bacillus anthracis Spores in Suspicious White Powders and Environmental Samples.	Ramage JG, Prentice KW, DePalma L, Venkateswaran KS, Chivukula S, Chapman C, Bell M, Datta S, Singh A, Hoffmaster A, Sarwar J, Parameswaran N, Joshi M, Thirunavkkarasu N, Krishnan V, Morse S, Avila JR, Sharma S, Estacio PL, Stanker L, Hodge DR, Pillai SP.	Health Secur	10.1089/hs.2016.0041	2016
	Vertical transmission of endobacteria in the arbuscular mycorrhizal fungus Gigaspora margarita through generation of vegetative spores.	Bianciotto V, Genre A, Jargeat P, Lumini E, Becard G, Bonfante P.	Appl Environ Microbiol	10.1128/aem.70.6.3600-3608.2004	2004
Metabolism	Ralstonia solanacearum iron scavenging by the siderophore staphyloferrin B is controlled by PhcA, the global virulence regulator.	Bhatt G, Denny TP.	J Bacteriol	10.1128/jb.186.23.7896-7904.2004	2004
	In Vitro Evaluation of Common Antimicrobial Solutions Used for Breast Implant Soaking and Breast Pocket Irrigation-Part 1: Efficacy Against Planktonic Bacteria.	Jewell ML, Hariri S, Lantz EE, Jewell HL, Strickland AD, Leung BK.	Aesthet Surg J	10.1093/asj/sjaa309	2021
Metabolism	Stable-Isotope Probing-Enabled Cultivation of the Indigenous Bacterium Ralstonia sp. Strain M1, Capable of Degrading Phenanthrene and Biphenyl in Industrial Wastewater.	Li J, Luo C, Zhang D, Cai X, Jiang L, Zhang G.	Appl Environ Microbiol	10.1128/aem.00511-19	2019
	In Vitro Evaluation of Common Antimicrobial Solutions Used for Breast Implant Soaking and Breast Pocket Irrigation-Part 2: Efficacy Against Biofilm-Associated Bacteria.	Jewell ML, Bionda N, Moran AV, Bevels EJ, Jewell HL, Hariri S, Leung BK.	Aesthet Surg J	10.1093/asj/sjaa308	2021
	Evaluation of sponge wipe surface sampling for collection of potential surrogates for non-spore-forming bioterrorism agents.	Aslett LD, Calfee MW, Monge M, Abdel-Hady A, Chamberlain T, Baartmans R, Touati A.	J Appl Microbiol	10.1093/jambio/lxae097	2024
Phylogeny	A Cross-section Metagenomics and 16S Ribosomal DNA Based Evaluation of the Bacterial and Archaeal Communities Resident in the Forumad Chromite Mine, Northeastern of Iran.	Mousavi Maleki MS, Yakhchali B, Karkhaneh AA, Rezvani M, Ahmadpour F.	Iran J Biotechnol	10.30498/ijb.2022.240607.2818	2022
	Surface Coating with Hyaluronic Acid-Gelatin-Crosslinked Hydrogel on Gelatin-Conjugated Poly(dimethylsiloxane) for Implantable Medical Device-Induced Fibrosis.	Joo H, Park J, Sutthiwanjampa C, Kim H, Bae T, Kim W, Choi J, Kim M, Kang S, Park H.	Pharmaceutics	10.3390/pharmaceutics13020269	2021
	Bacterial Adhesion and Biofilm Formation on Textured Breast Implant Shell Materials.	James GA, Boegli L, Hancock J, Bowersock L, Parker A, Kinney BM.	Aesthetic Plast Surg	10.1007/s00266-018-1234-7	2019
	Fluorescence-based rapid detection of microbiological contaminants in water samples.	Meder H, Baumstummler A, Chollet R, Barrier S, Kukuczka M, Olivieri F, Welterlin E, Beguin V, Ribault S.	ScientificWorldJournal	10.1100/2012/234858	2012
	rpsU-based discrimination within the genus Burkholderia.	Frickmann H, Neubauer H, Loderstaedt U, Derschum H, Hagen RM.	Eur J Microbiol Immunol (Bp)	10.1556/eujmi.4.2014.2.3	2014
	The Isolation and Characterization of a Broad Host Range Bcep22-like Podovirus JC1.	Davis CM, Ruest MK, Cole JH, Dennis JJ.	Viruses	10.3390/v14050938	2022
	Biochemical-genetic characterization and distribution of OXA-22, a chromosomal and inducible class D beta-lactamase from Ralstonia (Pseudomonas) pickettii.	Nordmann P, Poirel L, Kubina M, Casetta A, Naas T.	Antimicrob Agents Chemother	10.1128/aac.44.8.2201-2204.2000	2000
Phylogeny	Fluorescence in situ hybridization for rapid identification of Achromobacter xylosoxidans and Alcaligenes faecalis recovered from cystic fibrosis patients.	Wellinghausen N, Wirths B, Poppert S.	J Clin Microbiol	10.1128/jcm.00508-06	2006
Enzymology	OXA-60, a chromosomal, inducible, and imipenem-hydrolyzing class D beta-lactamase from Ralstonia pickettii.	Girlich D, Naas T, Nordmann P.	Antimicrob Agents Chemother	10.1128/aac.48.11.4217-4225.2004	2004
	Microbial population diversity in the urethras of healthy males and males suffering from nonchlamydial, nongonococcal urethritis.	Riemersma WA, van der Schee CJ, van der Meijden WI, Verbrugh HA, van Belkum A.	J Clin Microbiol	10.1128/jcm.41.5.1977-1986.2003	2003
Enzymology	Evaluation of Molecular Methods To Improve the Detection of Burkholderia pseudomallei in Soil and Water Samples from Laos.	Knappik M, Dance DA, Rattanavong S, Pierret A, Ribolzi O, Davong V, Silisouk J, Vongsouvath M, Newton PN, Dittrich S.	Appl Environ Microbiol	10.1128/aem.04204-14	2015
Phylogeny	Fourier transform infrared spectroscopy for rapid identification of nonfermenting gram-negative bacteria isolated from sputum samples from cystic fibrosis patients.	Bosch A, Minan A, Vescina C, Degrossi J, Gatti B, Montanaro P, Messina M, Franco M, Vay C, Schmitt J, Naumann D, Yantorno O.	J Clin Microbiol	10.1128/jcm.02267-07	2008
Phylogeny	Complete Genome Sequence of 3-Chlorobenzoate-Degrading Bacterium Cupriavidus necator NH9 and Reclassification of the Strains of the Genera Cupriavidus and Ralstonia Based on Phylogenetic and Whole-Genome Sequence Analyses.	Moriuchi R, Dohra H, Kanesaki Y, Ogawa N.	Front Microbiol	10.3389/fmicb.2019.00133	2019
Metabolism	The Legionella pneumophila Siderophore Legiobactin Is a Polycarboxylate That Is Identical in Structure to Rhizoferrin.	Burnside DM, Wu Y, Shafaie S, Cianciotto NP.	Infect Immun	10.1128/iai.00808-15	2015
Metabolism	Bactobolin resistance is conferred by mutations in the L2 ribosomal protein.	Chandler JR, Truong TT, Silva PM, Seyedsayamdost MR, Carr G, Radey M, Jacobs MA, Sims EH, Clardy J, Greenberg EP.	mBio	10.1128/mbio.00499-12	2012
Phylogeny	Species-specific PCR as a tool for the identification of Burkholderia gladioli.	Whitby PW, Pope LC, Carter KB, LiPuma JJ, Stull TL.	J Clin Microbiol	10.1128/jcm.38.1.282-285.2000	2000
Enzymology	Development of real-time PCR assays and evaluation of their potential use for rapid detection of Burkholderia pseudomallei in clinical blood specimens.	Supaprom C, Wang D, Leelayuwat C, Thaewpia W, Susaengrat W, Koh V, Ooi EE, Lertmemongkolchai G, Liu Y.	J Clin Microbiol	10.1128/jcm.00291-07	2007
	A highly selective PCR protocol for detecting 16S rRNA genes of the genus Pseudomonas (sensu stricto) in environmental samples.	Widmer F, Seidler RJ, Gillevet PM, Watrud LS, Di Giovanni GD.	Appl Environ Microbiol	10.1128/aem.64.7.2545-2553.1998	1998
Transcriptome	Quantitative detection of the Ralstonia solanacearum species complex in soil by qPCR combined with a recombinant internal control strain.	Chen W, Zhang J-W, Qin B-X, Xie H-T, Zhang Z, Qiao X-Z, Li S-K, Asif M, Guo S, Cui L-X, Wang P-P, Dong L-H, Guo Q-G, Jiang W-J, Ma P, Xia Z-Y, Lu C-H, Zhang L-Q.	Microbiol Spectr	10.1128/spectrum.00210-23	2023
Enzymology	Ralstonia infection in cystic fibrosis.	Green HD, Bright-Thomas R, Kenna DT, Turton JF, Woodford N, Jones AM.	Epidemiol Infect	10.1017/s0950268817001728	2017
Phylogeny	Development of real-time PCR assays for detection and quantification of Bacillus cereus group species: differentiation of B. weihenstephanensis and rhizoid B. pseudomycoides isolates from milk.	Oliwa-Stasiak K, Kolaj-Robin O, Adley CC.	Appl Environ Microbiol	10.1128/aem.01581-10	2011
Enzymology	Identification of Burkholderia spp. in the clinical microbiology laboratory: comparison of conventional and molecular methods.	van Pelt C, Verduin CM, Goessens WH, Vos MC, Tummler B, Segonds C, Reubsaet F, Verbrugh H, van Belkum A.	J Clin Microbiol	10.1128/jcm.37.7.2158-2164.1999	1999
Phylogeny	Matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of nonfermenting gram-negative bacilli isolated from cystic fibrosis patients.	Degand N, Carbonnelle E, Dauphin B, Beretti JL, Le Bourgeois M, Sermet-Gaudelus I, Segonds C, Berche P, Nassif X, Ferroni A.	J Clin Microbiol	10.1128/jcm.00569-08	2008
	Validation and Application of a Real-time PCR Protocol for the Specific Detection and Quantification of Clavibacter michiganensis subsp. sepedonicus in Potato.	Cho MS, Park DH, Namgung M, Ahn TY, Park DS.	Plant Pathol J	10.5423/ppj.oa.02.2015.0019	2015
	Paenibacillus larvae-Directed Bacteriophage HB10c2 and Its Application in American Foulbrood-Affected Honey Bee Larvae.	Beims H, Wittmann J, Bunk B, Sproer C, Rohde C, Gunther G, Rohde M, von der Ohe W, Steinert M.	Appl Environ Microbiol	10.1128/aem.00804-15	2015
	Tube-Wise Diagnostic Microarray for the Multiplex Characterization of the Complex Plant Pathogen Ralstonia solanacearum.	Cellier G, Arribat S, Chiroleu F, Prior P, Robene I.	Front Plant Sci	10.3389/fpls.2017.00821	2017
Enzymology	Low rates of Pseudomonas aeruginosa misidentification in isolates from cystic fibrosis patients.	Kidd TJ, Ramsay KA, Hu H, Bye PT, Elkins MR, Grimwood K, Harbour C, Marks GB, Nissen MD, Robinson PJ, Rose BR, Sloots TP, Wainwright CE, Bell SC, ACPinCF Investigators.	J Clin Microbiol	10.1128/jcm.00014-09	2009
	Anionic dye removal by immobilized bacteria into alginate-polyvinyl alcohol-bentonite matrix.	Purnomo AS, Hairunnisa FW, Misdar, Maria VP, Rohmah AA, Putra SR, Putro HS, Rizqi HD.	Heliyon	10.1016/j.heliyon.2024.e27871	2024
	The effect of bacteria addition on DDT biodegradation by BROWN-ROT fungus Gloeophyllum trabeum.	Rizqi HD, Purnomo AS, Ulfi A.	Heliyon	10.1016/j.heliyon.2023.e18216	2023
	The effect of Ralstonia pickettii bacterium addition on methylene blue dye biodecolorization by brown-rot fungus Daedalea dickinsii.	Nabilah B, Purnomo AS, Rizqi HD, Putro HS, Nawfa R.	Heliyon	10.1016/j.heliyon.2022.e08963	2022
Biotechnology	Synergistic interaction of a consortium of the brown-rot fungus Fomitopsis pinicola and the bacterium Ralstonia pickettii for DDT biodegradation.	Purnomo AS, Sariwati A, Kamei I.	Heliyon	10.1016/j.heliyon.2020.e04027	2020
Phylogeny	PCR Primer Design for 16S rRNAs for Experimental Horizontal Gene Transfer Test in Escherichia coli.	Miyazaki K, Sato M, Tsukuda M.	Front Bioeng Biotechnol	10.3389/fbioe.2017.00014	2017
	First Record of Microbiomes of Sponges Collected From the Persian Gulf, Using Tag Pyrosequencing.	Najafi A, Moradinasab M, Nabipour I.	Front Microbiol	10.3389/fmicb.2018.01500	2018
	Draft Genome Assembly of Ralstonia pickettii Type Strain K-288 (ATCC 27853).	Daligault HE, Davenport KW, Minogue TD, Broomall SM, Bruce DC, Chain PS, Coyne SR, Gibbons HS, Jaissle J, Lo CC, Meincke L, Munk AC, Rosenzweig CN, Johnson SL	Genome Announc	10.1128/genomeA.00973-14	2014
Genetics	Structure of the O-specific polysaccharide from Burkholderia pickettii strain NCTC 11149.	Galbraith L, George R, Wyklicky J, Wilkinson SG	Carbohydr Res	10.1016/0008-6215(95)00387-8	1996
Phylogeny	Transfer of two Burkholderia and an Alcaligenes species to Ralstonia gen. Nov.: Proposal of Ralstonia pickettii (Ralston, Palleroni and Doudoroff 1973) comb. Nov., Ralstonia solanacearum (Smith 1896) comb. Nov. and Ralstonia eutropha (Davis 1969) comb. Nov.	Yabuuchi E, Kosako Y, Yano I, Hotta H, Nishiuchi Y	Microbiol Immunol	10.1111/j.1348-0421.1995.tb03275.x	1995
Phylogeny	Flammeovirga pacifica sp. nov., isolated from deep-sea sediment.	Xu H, Fu Y, Yang N, Ding Z, Lai Q, Zeng R.	Int J Syst Evol Microbiol	10.1099/ijs.0.030676-0	2012
Phylogeny	Perexilibacter aurantiacus gen. nov., sp. nov., a novel member of the family 'Flammeovirgaceae' isolated from sediment.	Yoon J, Ishikawa S, Kasai H, Yokota A.	Int J Syst Evol Microbiol	10.1099/ijs.0.64845-0	2007

References

#2581	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 6297
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#22943	P. Vandamme, J. Goris, T. Coenye, B. Hoste, D. Janssens, K. Kersters, P. De Vos, E. Falsen: Assignment of Centers for Disease Control group IVc-2 to the genus Ralstonia as Ralstonia paucula sp. nov.. IJSEM 49: 663 - 669 1999 ( DOI 10.1099/00207713-49-2-663 , PubMed 10319489 )
#22944	T De Baere, S Steyaert, G Wauters, P Des Vos, J Goris, T Coenye, T Suyama, G Verschraegen, M Vaneechoutte: Classification of Ralstonia pickettii biovar 3/'thomasii' strains (Pickett 1994) and of new isolates related to nosocomial recurrent meningitis as Ralstonia mannitolytica sp. nov.. IJSEM 51: 547 - 558 2001 ( DOI 10.1099/00207713-51-2-547 , PubMed 11321101 )
#36535	; Curators of the CIP;
#44638	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 3318
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#121820	Collection of Institut Pasteur ; Curators of the CIP; CIP 73.23
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Ralstonia pickettii (DSM 6297, ATCC 27511, CIP 73.23)

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