Name: Mobiluncus curtisii BV345-16
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 208

Type strain:

Species: Mobiluncus curtisii

Strain Designation: BV345-16

Culture col. no.: DSM 23059, ATCC 35241, CCUG 21018, NCTC 11656

Strain history: <- CCUG; CCUG 21018 <- NCTC; NCTC 11656 <- C. A. Spiegel, MCW - Medical College of Wisconsin,USA; BV345-16

NCBI tax ID(s): 585198 (strain), 2051 (species)

SI-ID 45004

ATCC 35241, CCUG 21018, NCTC 11656, KCTC 5093, CNCTC 6670, DSM 23059

Special Collections

Other strains from Mobiluncus curtisii

Section

Mobiluncus curtisii BV345-16 is an anaerobe, Gram-variable, motile bacterium that was isolated from vaginae of women with bacterial vaginosis.

Gram-variable
motile
rod-shaped
anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Actinomycetales*
	Family	*Actinomycetaceae*
	Genus	*Mobiluncus*
	Species	**Mobiluncus curtisii**
	Full Scientific Name (LPSN)	Mobiluncus curtisii Spiegel and Roberts 1984

Information on morphological and physiological properties Morphology

[Ref.: #22975]	Gram stain	variable

[Ref.: #22975]	Cell length	1.7 µm

[Ref.: #22975]	Cell width	<0.5 µm

[Ref.: #22975]	Cell shape	rod-shaped

[Ref.: #22975]	Motility	yes

[Ref.: #22975]	Flagellum arrangement	lophotrichous

[Ref.: #16682]	Type of hemolysis	gamma

[Ref.: #22975]	Colony size	2.0-3.0 mm
[Ref.: #22975]	Colony color	colorless
[Ref.: #22975]	Incubation period	5 days

[Ref.: #47252]	Incubation period	4-8 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16682]

Culture medium

PYG MEDIUM (MODIFIED) (DSMZ Medium 104)

[Ref.: #16682]

Culture medium growth

[Ref.: #16682]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16682]

Culture medium composition

expand / minimize

[Ref.: #16682]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #16682]

Culture medium growth

[Ref.: #16682]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16682]

Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #16682]		growth	37 °C
[Ref.: #22975]		growth	35.0 °C
[Ref.: #22975]		growth	42.0 °C
[Ref.: #22975]	no	growth	45.0 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #16682]	Oxygen tolerance	anaerobe
[Ref.: #22975]	Oxygen tolerance	anaerobe

[Ref.: #22975]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #22975]	amygdalin ChEBI	-	builds acid from
[Ref.: #22975]	arabinose ChEBI	-	fermentation
[Ref.: #22975]	arginine ChEBI	-	builds acid from
[Ref.: #22975]	arginine ChEBI	+	growth
[Ref.: #68380]	arginine ChEBI	+	hydrolysis	* from API rID32A
[Ref.: #22975]	cellobiose ChEBI	-	builds acid from
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #22975]	erythritol ChEBI	-	builds acid from
[Ref.: #22975]	esculin ChEBI	-	builds acid from
[Ref.: #22975]	esculin ChEBI	-	hydrolysis
[Ref.: #22975]	fructose ChEBI	-	fermentation
[Ref.: #22975]	galactose ChEBI	+	fermentation
[Ref.: #22975]	glucose ChEBI	+	fermentation
[Ref.: #22975]	glycogen ChEBI	+	fermentation
[Ref.: #22975]	inulin ChEBI	-	builds acid from
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #22975]	lactose ChEBI	-	fermentation
[Ref.: #22975]	maltose ChEBI	+	fermentation
[Ref.: #22975]	mannitol ChEBI	-	builds acid from
[Ref.: #22975]	mannose ChEBI	-	fermentation
[Ref.: #22975]	melezitose ChEBI	-	builds acid from
[Ref.: #22975]	melibiose ChEBI	+	fermentation
[Ref.: #22975]	myo-inositol ChEBI	-	fermentation
[Ref.: #22975]	nitrate ChEBI	-	reduction
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #22975]	raffinose ChEBI	-	fermentation
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #22975]	rhamnose ChEBI	-	fermentation
[Ref.: #22975]	ribitol ChEBI	-	builds acid from
[Ref.: #22975]	ribose ChEBI	-	fermentation
[Ref.: #22975]	salicin ChEBI	-	builds acid from
[Ref.: #22975]	sorbitol ChEBI	-	builds acid from
[Ref.: #22975]	starch ChEBI	-	fermentation
[Ref.: #22975]	starch ChEBI	+	hydrolysis
[Ref.: #22975]	sucrose ChEBI	-	fermentation
[Ref.: #22975]	trehalose ChEBI	-	fermentation
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #22975]	xylose ChEBI	+	fermentation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #22975]	elemental sulfur ChEBI	no
[Ref.: #22975]	indole ChEBI	no
[Ref.: #68380]	indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68380]	alanine arylamidase	-	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	-	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #22975]	alpha-fucosidase		3.2.1.51
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #22975]	alpha-glucosidase	+	3.2.1.20
[Ref.: #22975]	alpha-mannosidase	-	3.2.1.24
[Ref.: #68380]	arginine dihydrolase	+	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #22975]	beta-glucosidase	-	3.2.1.21
[Ref.: #68380]	beta-glucosidase	-	3.2.1.21	* from API rID32A
[Ref.: #22975]	beta-glucuronidase	-	3.2.1.31
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #22975]	carboxylesterase	-	3.1.1.1
[Ref.: #22975]	catalase	-	1.11.1.6
[Ref.: #22975]	chymotrypsin	-	3.4.4.5
[Ref.: #22975]	cystine arylamidase	-	3.4.11.3
[Ref.: #22975]	cytochrome oxidase	-	1.9.3.1
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	+		* from API rID32A
[Ref.: #22975]	lecithinase	-
[Ref.: #22975]	leucine arylamidase	+	3.4.11.1
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #22975]	lipase	-
[Ref.: #22975]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32A
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #22975]	phosphoamidase	+	3.9.1.1
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #22975]	trypsin	-	3.4.21.4
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
[Ref.: #22975]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #47252]

Fatty acid	Percentage	ECL
C16:0	34.4	16
C18:2 ω6,9c/C18:0 ANTE	18.8	17.724
C18:1 ω9c	17	17.769
C18:0	14.1	18
C18:1 ω9c DMA	4.9	18.226
unknown 18.177	4.7	18.177
C14:0	1.9	14
C16:1 ω7c	1.9	15.819
C17:0 anteiso	1.2	16.722
C17:1 ISO I/C16:0 DMA	1.2	16.481

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #16682]	13314	-	+	+	+/-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	+	+	-	-
[Ref.: #16682]	12422	-	+	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+/-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #22975]	Sample type/isolated from	vaginae of women with bacterial vaginosis

[Ref.: #47252]	Sample type/isolated from	Human vagina

[Ref.: #16682]	Sample type/isolated from	human vagina
[Ref.: #16682]	Geographic location (country and/or sea, region)	Seattle, WA
[Ref.: #16682]	Country	USA
[Ref.: #16682]	Country ISO 3 Code	USA
[Ref.: #16682]	Continent	North America
[Ref.: #16682]	Geographic location	47.6080°/-122.3350°
* marker position based on {}

Isolation sources categories

#Infection	#Disease	-
#Host	#Human	#Female
#Host Body-Site	#Urogenital tract	#Vagina

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ427623 (>99% sequence identity) for Mobiluncus from Microbeatlas

Aquatic Samples	629
Soil Samples	819
Animal Samples	32804
Plant Samples	178
Total Samples	34430

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16682]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16682]	Mobiluncus curtisii subsp. curtisii partial 16S rRNA gene, strain CCUG 21018	AJ318408	1489	GenBank ENA	585198 tax ID
[Ref.: #20218]	Mobiluncus curtisii subsp. curtisii partial 16S rRNA gene, strain CCUG 21018T	AJ427623	1489	GenBank ENA	585198 tax ID	*
[Ref.: #20218]	M.curtisii 16S rRNA gene	X82603	369	GenBank ENA	585198 tax ID	*
[Ref.: #20218]	M.curtisii 16S rRNA gene (ssp. curtisii)	X86005	331	GenBank ENA	585198 tax ID	*
[Ref.: #124043]	Mobiluncus curtisii 16S rRNA (part.)	X53186	1463	GenBank ENA	585198 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Mobiluncus curtisii subsp. curtisii ATCC 35241	GCA_000146285	scaffold	GenBank ENA	585198 tax ID	*
[Ref.: #66792]	Mobiluncus curtisii subsp. curtisii NCTC11656	GCA_900453315	contig	GenBank ENA	144177 tax ID	*
[Ref.: #66792]	Mobiluncus curtisii strain FDAARGOS_1127	2051.55	complete	PATRIC	2051 tax ID	*
[Ref.: #66792]	Mobiluncus curtisii subsp. curtisii ATCC 35241	585198.7	wgs	PATRIC	585198 tax ID	*
[Ref.: #66792]	Mobiluncus curtisii subsp. curtisii strain NCTC11656	144177.3	wgs	PATRIC	144177 tax ID	*
[Ref.: #66792]	Mobiluncus curtisii curtisii ATCC 35241	648276689	draft	JGI IMG/M	585198 tax ID	*

[Ref.: #22975]

GC-content

51-52 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Mobiluncus curtisii strain FDAARGOS_1127 strain Not applicable PATRIC: 2051.55
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Mobiluncus curtisii strain FDAARGOS_1127 strain Not applicable PATRIC: 2051.55
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	59.907	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	yes	63.84	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	74.421	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	87.035	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	89.688	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	64.125	yes

Availability in culture collections External links

[Ref.: #16682]

Culture collection no.

DSM 23059, ATCC 35241, CCUG 21018, NCTC 11656

[Ref.: #69891]

SI-ID 45004

Literature:

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Diversity of Mobiluncus strains as demonstrated by their electrophoretic protein patterns.	Drouet EB, Boude M, Denoyel GA	Zentralbl Bakteriol	10.1016/s0934-8840(11)80213-6	1991	*
Pathogenicity	Characterisation of monoclonal antibodies for detection of Mobiluncus spp. in genital specimens.	Ison CA, Kolator B, Reid JH, Dermott E, Clark J, Easmon CS	J Med Microbiol	10.1099/00222615-30-2-129	1989	*

References

#16682

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23059

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#22975

Carol A. Spiegel,Marilyn Roberts: Mobiluncus gen. nov., Mobiluncus curtisii subsp. curtisii sp. nov., Mobiluncus curtisii subsp. holmesii subsp. nov., and Mobiluncus mulieris sp. nov., Curved Rods from the human Vagina. IJSEM 34: 177 - 184 1984 ( DOI 10.1099/00207713-34-2-177 )

#47252 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 21018

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#69891

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID45004.1 )

#124043

Dr. Isabel Schober, Dr. Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

* These data were automatically processed and therefore are not curated

Change proposal

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Mobiluncus curtisii strain FDAARGOS_1127 strain Not applicable

Mobiluncus curtisii strain FDAARGOS_1127 strain Not applicable

Change proposal

Change proposal