Polynucleobacter duraquae (DSM 21495, CIP 110977, MWH-MoK4)

Name: Polynucleobacter duraquae (DSM 21495, CIP 110977, MWH-MoK4)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2071

Type strain

Strain Designation MWH-MoK4

Culture col. no. DSM 21495 CIP 110977 MWH-MoK4

NCBI tax ID(s) 1835254

Special Collections DSMZ CIP Literature strains

Links

Polynucleobacter duraquae MWH-MoK4 is an aerobe, chemoorganotroph, psychrophilic prokaryote that was isolated from water of freshwater lake.

rod-shaped aerobe chemoorganotroph psychrophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 405384

DSM 21495,CIP 110977

@ref 20215

Last LPSN update 2025-12-16 09:47:15

Domain Pseudomonadati

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Burkholderiaceae

Genus Polynucleobacter

Species Polynucleobacter duraquae

Full scientific name Polynucleobacter duraquae Hahn et al. 2016

Morphology

Cell morphology

@ref	Cell length	Cell width	Cell shape
43586	0.9-2.9 µm	0.4-0.5 µm	rod-shaped
118981			rod-shaped
125439
125438

Colony morphology

@ref	Incubation period
15746	>14 days
43586

Culture and growth conditions

Culture medium

@ref	Name	Composition
41645	MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromati	Distilled water make up to (1000.000 ml);R2A agar (18.200 g)
43586	Luria-Bertani medium
43586	NSY
43586	Peptone
43586	Reasoner's 2A agar (R2A)
43586	Yeast extract
15746	R2A MEDIUM (DSMZ Medium 830)	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
118981	CIP Medium 566

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
15746	positive	growth	15	psychrophilic
41645	positive	growth	20	psychrophilic
43586	positive	growth	30	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
43586	aerobe
125439	obligate aerobe

Nutrition type

43586

Typechemoorganotroph

Spore formation

@ref	Spore formation	Confidence
125439		99.8
125438		90.984

Halophily

@ref	Salt	Growth	Tested relation	Concentration
43586	NaCl	positive	growth	0-0.3 %(w/v)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43586	39150 ChEBI	4-oxopentanoate	-	assimilation
43586	30089 ChEBI	acetate	+	assimilation
43586	16947 ChEBI	citrate	-	assimilation
43586	12936 ChEBI	D-galactose	-	assimilation
43586	16024 ChEBI	D-mannose	-	assimilation
43586	17924 ChEBI	D-sorbitol	-	assimilation
43586	29806 ChEBI	fumarate	+	assimilation
43586	29805 ChEBI	glycolate	-	assimilation
43586	36655 ChEBI	glyoxylate	-	assimilation
43586	16977 ChEBI	L-alanine	-	assimilation
43586	17196 ChEBI	L-asparagine	-	assimilation
43586	29991 ChEBI	L-aspartate	-	assimilation
43586	17561 ChEBI	L-cysteine	+	assimilation
43586	18287 ChEBI	L-fucose	-	assimilation
43586	17115 ChEBI	L-serine	-	assimilation
43586	15792 ChEBI	malonate	-	assimilation
43586	30623 ChEBI	oxalate	-	assimilation
43586	16452 ChEBI	oxaloacetate	+	assimilation
43586	17272 ChEBI	propionate	-	assimilation
43586	15361 ChEBI	pyruvate	+	assimilation
43586	64243 ChEBI	sodium L-glutamate	-	assimilation
43586	30031 ChEBI	succinate	+	assimilation

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Freshwater
#Condition	#Alkaline	-
#Environmental	#Aquatic	#Lake (large)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude
15746	water of freshwater lake	Lake Mondsee (47° 49' 47.11" N 13° 22' 33.94"E)	Austria	AUT	Europe	47.8297	13.3761 47.8297/13.3761
43586	Alkaline Lake Mondsee	Salzkammergut, Austria	Austria	AUT	Europe
118981	Environment, Freshwater from pelagic zone of Mondsee lake	Mondsee lake	Austria	AUT	Europe

Taxonmaps

69479

Global distribution of 16S sequence AJ550654 (>99% sequence identity) for Polynucleobacter from Microbeatlas

Aquatic Samples	34169
Soil Samples	1815
Animal Samples	6310
Plant Samples	555
Total Samples	42849

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
15746	1	Risk group (German classification) According to TRBA 466
118981	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM97362v1 assembly for Polynucleobacter duraquae MWH-MoK4	complete	GCA_000973625	576611.7	2634166443	1835254	94.67

Genome browser: GCA_000973625

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
15746	Polynucleobacter duraquae partial 16S rRNA gene and ITS1, isolate MWH-MoK4	AJ550654	2014		1835254

GC content

@ref	GC-content (mol%)
43586	45.2
15746	45.2

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Polynucleobacter necessarius subsp. asymbioticus strain MWH-MoK4
PATRIC: 576611.7

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	99.80	no
125439	motility	BacteriaNetⓘ	yes	83.70	no
125439	gram_stain	BacteriaNetⓘ	negative	99.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	94.30	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Polynucleobacter duraquae MWH-MoK4
NCBI: GCA_000973625.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	94.83	no
125438	anaerobic	anaerobicⓘ	no	91.25	no
125438	aerobic	aerobicⓘ	yes	83.38	yes
125438	spore-forming	spore-formingⓘ	no	90.98	no
125438	thermophilic	thermophileⓘ	no	95.44	yes
125438	flagellated	motile2+ⓘ	no	58.22	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Reclassification of four Polynucleobacter necessarius strains as representatives of Polynucleobacter asymbioticus comb. nov., Polynucleobacter duraquae sp. nov., Polynucleobacter yangtzensis sp. nov. and Polynucleobacter sinensis sp. nov., and emended description of Polynucleobacter necessarius.	Hahn MW, Schmidt J, Pitt A, Taipale SJ, Lang E	Int J Syst Evol Microbiol	10.1099/ijsem.0.001073	2016

References

#15746	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21495
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#41645	; Curators of the CIP;
#43586	Martin W. Hahn, Johanna Schmidt, Alexandra Pitt, Sami J. Taipale, Elke Lang: Reclassification of four Polynucleobacter necessarius strains as representatives of Polynucleobacter asymbioticus comb. nov., Polynucleobacter duraquae sp. nov., Polynucleobacter yangtzensis sp. nov. and Polynucleobacter sinensis sp. nov., and emended description of Polynucleobacter necessarius. IJSEM 66: 2883 - 2892 2016 ( DOI 10.1099/ijsem.0.001073 , PubMed 27064460 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#118981	Collection of Institut Pasteur ; Curators of the CIP; CIP 110977
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Polynucleobacter duraquae (DSM 21495, CIP 110977, MWH-MoK4)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Nutrition type

Spore formation

Halophily

Metabolite utilization

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000973625

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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