Name: Polynucleobacter sinensis MWH-HuW1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2069

Type strain:

Species: Polynucleobacter sinensis

Strain Designation: MWH-HuW1

Culture col. no.: DSM 21492, CIP 110978

Strain history: CIP <- 2015, DSMZ <- M.W. Hahn, Austrian Academy of Science, Mondsee, Austria: strain MWH-HuW1

NCBI tax ID(s): 1743157 (species)

SI-ID 402770

DSM 21492, CIP 110978

Section

Polynucleobacter sinensis MWH-HuW1 is an aerobe, chemoorganotroph, mesophilic bacterium that forms circular colonies and was isolated from water from a slightly alkaline, artificial pond.

colony-forming
rod-shaped
aerobe
chemoorganotroph
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Burkholderiaceae*
	Genus	*Polynucleobacter*
	Species	**Polynucleobacter sinensis**
	Full Scientific Name (LPSN)	Polynucleobacter sinensis Hahn et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #122628]	Gram stain	negative

[Ref.: #122628]	Cell shape	rod-shaped
[Ref.: #43586]	Cell shape	rod-shaped

[Ref.: #122628]	Motility	yes

[Ref.: #43586]	Cell length	0.6-1.4 µm

[Ref.: #43586]	Cell width	0.4-0.5 µm

[Ref.: #43586]	Colony color	non-pigmented
[Ref.: #43586]	Colony shape	circular
[Ref.: #43586]	Cultivation medium used	NSY

Information on culture and growth conditions Culture and growth conditions

[Ref.: #42010]

Culture medium

MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromati

[Ref.: #42010]

Culture medium growth

[Ref.: #42010]

Culture medium composition

expand / minimize

[Ref.: #43586]

Culture medium

NSY

[Ref.: #43586]

Culture medium growth

[Ref.: #43586]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #43586]

Culture medium growth

[Ref.: #15743]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #15743]

Culture medium growth

[Ref.: #15743]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15743]

Culture medium composition

expand / minimize

[Ref.: #122628]

Culture medium

CIP Medium 566

[Ref.: #122628]

Culture medium growth

[Ref.: #122628]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #15743]	growth	28 °C
[Ref.: #42010]	growth	25 °C
[Ref.: #43586]	growth	35 °C

[Ref.: #15743]	Temperature range	mesophilic
[Ref.: #42010]	Temperature range	mesophilic
[Ref.: #43586]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43586]

Oxygen tolerance

aerobe

[Ref.: #43586]

Nutrition type

chemoorganotroph

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43586]		NaCl		growth	0-0.5	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43586]	4-oxopentanoate ChEBI	-	assimilation
[Ref.: #43586]	acetate ChEBI	+	assimilation
[Ref.: #43586]	citrate ChEBI	-	assimilation
[Ref.: #43586]	D-fructose ChEBI	-	assimilation
[Ref.: #43586]	D-galactose ChEBI	-	assimilation
[Ref.: #43586]	D-glucose ChEBI	-	assimilation
[Ref.: #43586]	D-lyxose ChEBI	-	assimilation
[Ref.: #43586]	D-mannose ChEBI	-	assimilation
[Ref.: #43586]	D-sorbitol ChEBI	-	assimilation
[Ref.: #43586]	fumarate ChEBI	+	assimilation
[Ref.: #43586]	glycolate ChEBI	-	assimilation
[Ref.: #43586]	glyoxylate ChEBI	-	assimilation
[Ref.: #43586]	L-alanine ChEBI	-	assimilation
[Ref.: #43586]	L-asparagine ChEBI	-	assimilation
[Ref.: #43586]	L-aspartate ChEBI	-	assimilation
[Ref.: #43586]	L-fucose ChEBI	-	assimilation
[Ref.: #43586]	L-glutamate ChEBI	+	assimilation
[Ref.: #43586]	L-serine ChEBI	-	assimilation
[Ref.: #43586]	malate ChEBI	+	assimilation
[Ref.: #43586]	malonate ChEBI	+	assimilation
[Ref.: #43586]	oxalate ChEBI	-	assimilation
[Ref.: #43586]	oxaloacetate ChEBI	+	assimilation
[Ref.: #43586]	propionate ChEBI	+	assimilation
[Ref.: #43586]	pyruvate ChEBI	+	assimilation
[Ref.: #43586]	succinate ChEBI	+	assimilation
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15743]	Sample type/isolated from	water from a slightly alkaline, artificial pond
[Ref.: #15743]	Geographic location (country and/or sea, region)	Suzhou, Tiger Hill (Huqiu) 31° 20' 14.81'' N 120° 34' 34.20'' E
[Ref.: #15743]	Country	China
[Ref.: #15743]	Country ISO 3 Code	CHN
[Ref.: #15743]	Continent	Asia
[Ref.: #15743]	Geographic location	31.3375°/120.5760°

[Ref.: #43586]	Sample type/isolated from	a slightly alkaline, artificial pond
[Ref.: #43586]	Geographic location (country and/or sea, region)	Tiger Hill (Huqiu), Suzhou
[Ref.: #43586]	Country	China
[Ref.: #43586]	Country ISO 3 Code	CHN
[Ref.: #43586]	Continent	Asia
[Ref.: #43586]	Geographic location	31.3370°/120.5760°

[Ref.: #122628]	Sample type/isolated from	Pond in Hugin park
[Ref.: #122628]	Country	China
[Ref.: #122628]	Country ISO 3 Code	CHN
[Ref.: #122628]	Continent	Asia
[Ref.: #122628]	Isolation date	2002-11-20
* marker position based on {}

Isolation sources categories

#Condition	#Alkaline	-
#Environmental	#Aquatic	#Pond (small)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ550666 (>99% sequence identity) for Polynucleobacter from Microbeatlas

Aquatic Samples	34169
Soil Samples	1815
Animal Samples	6310
Plant Samples	555
Total Samples	42849

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15743]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #122628]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15743]	Polynucleobacter necessarius subsp. asymbioticus partial 16S rRNA gene and ITS1, isolate MWH-HuW1	AJ550666	2014	ENA	1743157 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Polynucleobacter sinensis MWH-HuW1	GCA_001595985	scaffold	GenBank	1743157 tax ID	*
[Ref.: #66792]	Polynucleobacter sinensis MWH-HuW1	2844340359	complete	JGI IMG/M	1743157 tax ID	*

[Ref.: #43586]	GC-content	45.5 mol%
[Ref.: #15743]	GC-content	45.5 mol%	sequence analysis

Availability in culture collections External links

[Ref.: #15743]

Culture collection no.

DSM 21492, CIP 110978

[Ref.: #71716]

SI-ID 402770

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Reclassification of four Polynucleobacter necessarius strains as representatives of Polynucleobacter asymbioticus comb. nov., Polynucleobacter duraquae sp. nov., Polynucleobacter yangtzensis sp. nov. and Polynucleobacter sinensis sp. nov., and emended description of Polynucleobacter necessarius.	Hahn MW, Schmidt J, Pitt A, Taipale SJ, Lang E	Int J Syst Evol Microbiol	10.1099/ijsem.0.001073	2016	*

References

#15743

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21492

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#42010 ; Curators of the CIP;

#43586

Martin W. Hahn, Johanna Schmidt, Alexandra Pitt, Sami J. Taipale, Elke Lang: Reclassification of four Polynucleobacter necessarius strains as representatives of Polynucleobacter asymbioticus comb. nov., Polynucleobacter duraquae sp. nov., Polynucleobacter yangtzensis sp. nov. and Polynucleobacter sinensis sp. nov., and emended description of Polynucleobacter necessarius. IJSEM 66: 2883 - 2892 2016 ( DOI 10.1099/ijsem.0.001073 , PubMed 27064460 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#71716

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID402770.1 )

#122628 Collection of Institut Pasteur ; Curators of the CIP; CIP 110978
* These data were automatically processed and therefore are not curated

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