Name: Polynucleobacter asymbioticus QLW-P1DMWA-1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2068

Type strain:

Species: Polynucleobacter asymbioticus

Strain Designation: QLW-P1DMWA-1

Culture col. no.: DSM 18221, CIP 109841

Strain history: CIP <- 2008, DSMZ <- M.W. Hahn, Austrian Science Academy, Mondsee, Austria: strain QLW-P1DMWA-1

NCBI tax ID(s): 576611 (species)

SI-ID 364175

CIP 109841, DSM 18221, LMG 24810

Other strains from Polynucleobacter asymbioticus

P. asymbioticus JCM 16613
P. asymbioticus JCM 16614
P. asymbioticus JCM 16615

Section

Polynucleobacter asymbioticus QLW-P1DMWA-1 is a facultative anaerobe, chemoorganotroph, mesophilic bacterium that forms circular colonies and was isolated from acidic freshwater pond.

colony-forming
rod-shaped
chemoorganotroph
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Burkholderiaceae*
	Genus	*Polynucleobacter*
	Species	**Polynucleobacter asymbioticus**
	Full Scientific Name (LPSN)	Polynucleobacter asymbioticus (Hahn et al. 2009) Hahn et al. 2016

Synonym

Polynucleobacter necessarius subsp. asymbioticus

Information on morphological and physiological properties Morphology

[Ref.: #116533]	Gram stain	negative

[Ref.: #116533]	Cell shape	rod-shaped
[Ref.: #43586]	Cell shape	rod-shaped

[Ref.: #116533]	Motility	no

[Ref.: #43586]	Cell length	0.7-1.2 µm

[Ref.: #43586]	Cell width	0.4-0.5 µm

[Ref.: #43586]	Colony shape	circular
[Ref.: #43586]	Cultivation medium used	NSY agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7372]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #7372]

Culture medium growth

[Ref.: #7372]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7372]

Culture medium composition

expand / minimize

[Ref.: #41999]

Culture medium

MEDIUM 706- for Polynucleobacter

[Ref.: #41999]

Culture medium growth

[Ref.: #43586]

Culture medium

LB (Luria-Bertani) MEDIUM

[Ref.: #43586]

Culture medium growth

[Ref.: #43586]

Culture medium

NSY

[Ref.: #43586]

Culture medium growth

[Ref.: #43586]

Culture medium

NSY agar

[Ref.: #43586]

Culture medium growth

[Ref.: #43586]

Culture medium

Peptone medium

[Ref.: #43586]

Culture medium growth

[Ref.: #43586]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #43586]

Culture medium growth

[Ref.: #116533]

Culture medium

CIP Medium 706

[Ref.: #116533]

Culture medium growth

[Ref.: #116533]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #7372]	growth	28 °C
[Ref.: #41999]	growth	30 °C
[Ref.: #43586]	growth	34 °C

[Ref.: #7372]	Temperature range	mesophilic
[Ref.: #41999]	Temperature range	mesophilic
[Ref.: #43586]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43586]

Oxygen tolerance

facultative anaerobe

[Ref.: #43586]

Nutrition type

chemoorganotroph

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43586]		NaCl		growth	0-0.4	%(w/v)
[Ref.: #43586]		NaCl	no	growth	0.6	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43586]	acetate ChEBI	+	assimilation
[Ref.: #43586]	citrate ChEBI	-	assimilation
[Ref.: #43586]	D-galacturonic acid ChEBI	+	assimilation
[Ref.: #43586]	fumarate ChEBI	+	assimilation
[Ref.: #43586]	glycolate ChEBI	-	assimilation
[Ref.: #43586]	L-aspartate ChEBI	+	assimilation
[Ref.: #43586]	L-cysteine ChEBI	+	assimilation
[Ref.: #43586]	L-glutamate ChEBI	+	assimilation
[Ref.: #43586]	L-serine ChEBI	-	assimilation
[Ref.: #43586]	malate ChEBI	+	assimilation
[Ref.: #116533]	nitrate ChEBI	-	reduction
[Ref.: #116533]	nitrite ChEBI	-	reduction
[Ref.: #43586]	oxalate ChEBI	-	assimilation
[Ref.: #43586]	oxaloacetate ChEBI	-	assimilation
[Ref.: #43586]	propionate ChEBI	+	assimilation
[Ref.: #43586]	pyruvate ChEBI	+	assimilation
[Ref.: #43586]	succinate ChEBI	+	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #116533]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #116533]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #116533]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #116533]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116533]	12152	-	+	+	-	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7372]	Sample type/isolated from	acidic freshwater pond
[Ref.: #7372]	Geographic location (country and/or sea, region)	Alps, (47° 44' 27'' N 13° 17' 53'' E at 1300 meters above sea level)
[Ref.: #7372]	Country	Austria
[Ref.: #7372]	Country ISO 3 Code	AUT
[Ref.: #7372]	Continent	Europe
[Ref.: #7372]	Geographic location	47.7408°/13.2981°

[Ref.: #43586]	Sample type/isolated from	a small acidic freshwater pond located in the Austrian Alps at an altitude of 1300 m
[Ref.: #43586]	Geographic location (country and/or sea, region)	Austrian Alps
[Ref.: #43586]	Country	Austria
[Ref.: #43586]	Country ISO 3 Code	AUT
[Ref.: #43586]	Continent	Europe

[Ref.: #116533]	Sample type/isolated from	Environment, Freshwater pond
[Ref.: #116533]	Geographic location (country and/or sea, region)	Alpes
[Ref.: #116533]	Country	Austria
[Ref.: #116533]	Country ISO 3 Code	AUT
[Ref.: #116533]	Continent	Europe
[Ref.: #116533]	Isolation date	2003
* marker position based on {}

Isolation sources categories

#Condition	#Acidic	-
#Environmental	#Aquatic	#Freshwater
#Environmental	#Aquatic	#Pond (small)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ879783 (>99% sequence identity) for Polynucleobacter from Microbeatlas

Aquatic Samples	34169
Soil Samples	1815
Animal Samples	6310
Plant Samples	555
Total Samples	42849

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7372]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116533]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7372]	Polynucleobacter asymbioticus partial 16S rRNA gene and ITS1, strain QLW-P1DMWA-1	AJ879783	2000	ENA	312153 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Polynucleobacter asymbioticus QLW-P1DMWA-1	GCA_000016345	complete	GenBank	312153 tax ID	*
[Ref.: #66792]	Polynucleobacter asymbioticus QLW-P1DMWA-1	2639762516	complete	JGI IMG/M	312153 tax ID	*

[Ref.: #7372]	GC-content	44.8 mol%	high performance liquid chromatography (HPLC)
[Ref.: #43586]	GC-content	44.8 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Polynucleobacter asymbioticus QLW-P1DMWA-1 QLW-P1DMWA-1 NCBI: GCA_000016345.1
[Ref.: #69480]	motile	no	92.586	no
[Ref.: #69480]	flagellated	no	93.754	no
[Ref.: #69480]	gram-positive	no	98.555	no
[Ref.: #69480]	anaerobic	no	97.67	yes
[Ref.: #69480]	halophile	no	91.992	yes
[Ref.: #69480]	spore-forming	no	96.597	no
[Ref.: #69480]	thermophile	no	97.913	yes
[Ref.: #69480]	glucose-util	no	77.204	no
[Ref.: #69480]	aerobic	yes	83.728	no
[Ref.: #69480]	glucose-ferment	no	89.808	no

Availability in culture collections External links

[Ref.: #7372]

Culture collection no.

DSM 18221, CIP 109841

[Ref.: #71715]

SI-ID 364175

Literature:

Topic	Title	Authors	Journal	DOI	Year
Transcriptome	Combined Methylome, Transcriptome and Proteome Analyses Document Rapid Acclimatization of a Bacterium to Environmental Changes.	Srivastava A, Murugaiyan J, Garcia JAL, De Corte D, Hoetzinger M, Eravci M, Weise C, Kumar Y, Roesler U, Hahn MW, Grossart HP	Front Microbiol	10.3389/fmicb.2020.544785	2020	*
Phylogeny	Reclassification of four Polynucleobacter necessarius strains as representatives of Polynucleobacter asymbioticus comb. nov., Polynucleobacter duraquae sp. nov., Polynucleobacter yangtzensis sp. nov. and Polynucleobacter sinensis sp. nov., and emended description of Polynucleobacter necessarius.	Hahn MW, Schmidt J, Pitt A, Taipale SJ, Lang E	Int J Syst Evol Microbiol	10.1099/ijsem.0.001073	2016	*

References

#7372

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18221

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#41999 ; Curators of the CIP;

#43586

Martin W. Hahn, Johanna Schmidt, Alexandra Pitt, Sami J. Taipale, Elke Lang: Reclassification of four Polynucleobacter necessarius strains as representatives of Polynucleobacter asymbioticus comb. nov., Polynucleobacter duraquae sp. nov., Polynucleobacter yangtzensis sp. nov. and Polynucleobacter sinensis sp. nov., and emended description of Polynucleobacter necessarius. IJSEM 66: 2883 - 2892 2016 ( DOI 10.1099/ijsem.0.001073 , PubMed 27064460 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#71715

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID364175.1 )

#116533 Collection of Institut Pasteur ; Curators of the CIP; CIP 109841
* These data were automatically processed and therefore are not curated

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Polynucleobacter asymbioticus QLW-P1DMWA-1 QLW-P1DMWA-1

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