Wautersia eutropha (DSM 531, ATCC 17697, CCUG 1776)

Name: Wautersia eutropha (DSM 531, ATCC 17697, CCUG 1776)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2000

Type strain

Strain Designation 335, R-10-e

Culture col. no. DSM 531 ATCC 17697 CCUG 1776 LMG 1199 IMET 10383 18 more

NCBI tax ID(s) 106590

Special Collections DSMZ CCUG JCM CIP

History <- ATCC <- R.Y. Stanier, 335 (Hydrogenomonas eutropha) <- R. Repaske, R-10-e <- C. Bovell LMG 1199 <-- ATCC 17697 <-- R. Y. Stanier 335 <-- R. Repaske R-10-e <-- C. Bovell. CIP <- 1996, E. Yabuuchi, Osaka City Univ. Sch. Med., Osaka, Japan <- JCM <- IAM <- ATCC <- R.Y. Stanier: strain 335, Hydrogenomonas eutropha <- R. Repaske: strain R-10-e <- C. Bovell

Links

Wautersia eutropha 335 is an aerobe, mesophilic, Gram-negative prokaryote that was isolated from soil.

Gram-negative motile oval-shaped aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 13620

LMG 1199,ATCC 17697,CCUG 1776,DSM 531,IMET 10383,NCIB 11842,JCM 11282,CIP 104763,NCIMB 11842,JCM 9691,IAM 12368,LMD 82.41,CECT 4634,NRRL B-14690,NRRL B-4383,NCCB 82041,IAM 13533,KACC 10791,KACC 10467,BCRC 17389,CCRC 17389,NBRC 102504,CFBP 6735,JCM 20521,JCM 20893

@ref 20215

Last LPSN update 2025-12-16 09:47:14

Domain Pseudomonadati

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Burkholderiaceae

Genus Wautersia

Species Wautersia eutropha

Full scientific name Wautersia eutropha (Davis 1969) Vaneechoutte et al. 2004

Synonyms (2)

Alcaligenes eutrophus
Ralstonia eutropha

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
119146	negative	oval-shaped
125438	negative		97
125439	negative		98.5

Colony morphology

119146

Hemolysis ability1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
129	MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) (DSMZ Medium 81)	Medium recipe at MediaDive	Name: MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) (DSMZ Medium 81) Composition: Agar 20.1005 g/l Na2HPO4 x 2 H2O 2.91457 g/l KH2PO4 2.31156 g/l NH4Cl 1.00503 g/l MgSO4 x 7 H2O 0.502512 g/l Ferric ammonium citrate 0.0502513 g/l CaCl2 x 2 H2O 0.0100503 g/l NaVO3 x H2O 0.00502512 g/l Calcium pantothenate 0.00251256 g/l Pyridoxine hydrochloride 0.00251256 g/l Nicotinic acid 0.00251256 g/l Thiamine-HCl x 2 H2O 0.00251256 g/l H3BO3 0.00150754 g/l CoCl2 x 6 H2O 0.00100503 g/l Riboflavin 0.000502513 g/l ZnSO4 x 7 H2O 0.000502513 g/l MnCl2 x 4 H2O 0.000150754 g/l Na2MoO4 x 2 H2O 0.000150754 g/l NiCl2 x 6 H2O 0.000100503 g/l CuCl2 x 2 H2O 5.02513e-05 g/l Vitamin B12 5.02513e-05 g/l Folic acid 1.00503e-05 g/l Biotin 5.02513e-06 g/l Distilled water
129	NUTRIENT AGAR (DSMZ Medium 1)	Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
41806	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
119146	CIP Medium 72	Medium recipe at CIP
119146	CIP Medium 3	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
129	positive	growth	30	mesophilic
41806	positive	growth	30	mesophilic
44416	positive	growth	26-30	mesophilic
67770	positive	growth	30	mesophilic
119146	positive	growth	15-41
119146	negative	growth	5
119146	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
44416	aerobe
119146	obligate aerobe
125439	obligate aerobe	98.7

Spore formation

@ref	Spore formation	Confidence
125439		98.1

Compound production

@ref	Compound
129	PHB
129	hydrogenase
129	single cell protein

Halophily

@ref	Salt	Growth	Tested relation	Concentration
119146	NaCl	positive	growth	0-2 %
119146	NaCl		growth	4 %
119146	NaCl		growth	6 %
119146	NaCl		growth	8 %
119146	NaCl		growth	10 %

Observation

67770

Observationquinones: Q-8

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	+	assimilation	from API 20NE
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
119146	16947 ChEBI	citrate	+	carbon source
68369	17634 ChEBI	D-glucose	-	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
119146	4853 ChEBI	esculin	-	hydrolysis
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
119146	606565 ChEBI	hippurate	+	hydrolysis
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
119146	15792 ChEBI	malonate	-	assimilation
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
119146	17632 ChEBI	nitrate	+	reduction
119146	17632 ChEBI	nitrate	-	respiration
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
119146	16301 ChEBI	nitrite	+	reduction
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
119146	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE
119146	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test	Voges-proskauer-test	Methylred-test
119146	15688 ChEBI	acetoin		-
119146	17234 ChEBI	glucose			-
68369	35581 ChEBI	indole	-			from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
119146	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
119146	amylase	-
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68382	beta-galactosidase	-	3.2.1.23	from API zym
119146	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
68382	beta-glucuronidase	-	3.2.1.31	from API zym
119146	caseinase	-	3.4.21.50
119146	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
119146	DNase	-
68382	esterase (C 4)	+		from API zym
119146	gamma-glutamyltransferase	+	2.3.2.2
119146	gelatinase	-
68369	gelatinase	-		from API 20NE
119146	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
119146	lipase	-
68382	lipase (C 14)	-		from API zym
119146	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
119146	ornithine decarboxylase	-	4.1.1.17
119146	oxidase	+
119146	phenylalanine ammonia-lyase	-	4.3.1.24
119146	protease	+
68382	trypsin	-	3.4.21.4	from API zym
119146	tryptophan deaminase	-
119146	tween esterase	+
119146	urease	-	3.5.1.5
68369	urease	-	3.5.1.5	from API 20NE
68382	valine arylamidase	-		from API zym

Fatty acid profile

Metadata FA analysis

@ref

44416

Fatty acid	Percentage	ECL
C16:1 ω7c	41.5	15.819
C16:0	20.4	16
C14:0 3OH/C16:1 ISO I	16.4	15.485
C18:1 ω7c /12t/9t	12.9	17.824
C14:0	5.1	14
C14:0 2OH	3.7	15.205

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
44416	-	+	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-
119146	-	+	+	-	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
44416	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	+	+	+	+

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
119146	not determinedn.d.	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	+	+	-	-	+	-	+	+	+	+	+	+	-	+	+	+	-	-	-	-	-	+	-	+	+	+	+	+	+	+	+	-	-	-	-	+	+	+	+	+	-	+	+	-	+	+	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent	Isolation date
129	soil
44416	Soil	USA	USA	North America
67770	Soil
119146	Environment, Soil	United States of America	USA	North America	1957

Taxonmaps

69479

Global distribution of 16S sequence LC507442 (>99% sequence identity) for Cupriavidus from Microbeatlas

Aquatic Samples	5686
Soil Samples	15055
Animal Samples	7044
Plant Samples	2775
Total Samples	30560

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
129	1	Risk group (German classification) According to TRBA 466
119146	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM159875v1 assembly for Cupriavidus necator NBRC 102504	contig	GCA_001598755	1218105.7	2731957713	1218105	49.68

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
67770	Cupriavidus necator gene for 16S rRNA, partial sequence, strain: JCM 11282	AB594764	1454	106590
67770	Ralstonia eutropha DNA 16S ribosomal RNA	D88000	1468	106590
67770	Cupriavidus necator JCM 11282 gene for 16S ribosomal RNA, partial sequence	LC507442	1454	106590
67770	A.eutrophus small subunit ribosomal RNA	M32021	1511	106590
124043	Cupriavidus necator gene for 16S rRNA, partial sequence, strain: NBRC 102504.	AB681838	1457	106590
124043	Ralstonia eutropha 16S rRNA gene (partial), 23S rRNA gene (partial) and internal transcribed spacer 1 (ITS1), strain ATCC17697	AJ416498	538	106590

GC content

@ref	GC-content (mol%)	Method
129	66.8
67770	66.5	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Cupriavidus necator NBRC 102504
NCBI: GCA_001598755

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.70	no
125439	gram_stain	BacteriaNetⓘ	negative	98.50	no
125439	motility	BacteriaNetⓘ	yes	76.70	no
125439	spore_formation	BacteriaNetⓘ	no	98.10	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Cupriavidus necator NBRC 102504
PATRIC: 1218105.7

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	97.00	no
125438	anaerobic	anaerobicⓘ	no	97.39	yes
125438	spore-forming	spore-formingⓘ	no	88.33	no
125438	aerobic	aerobicⓘ	yes	89.93	yes
125438	thermophilic	thermophileⓘ	no	98.49	yes
125438	flagellated	motile2+ⓘ	yes	84.06	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	A Review on Enhancing Cupriavidus necator Fermentation for Poly(3-hydroxybutyrate) (PHB) Production From Low-Cost Carbon Sources.	Zhang L, Jiang Z, Tsui TH, Loh KC, Dai Y, Tong YW.	Front Bioeng Biotechnol	10.3389/fbioe.2022.946085	2022
	Production and Properties of Microbial Polyhydroxyalkanoates Synthesized from Hydrolysates of Jerusalem Artichoke Tubers and Vegetative Biomass.	Volova TG, Kiselev EG, Demidenko AV, Zhila NO, Nemtsev IV, Lukyanenko AV.	Polymers (Basel)	10.3390/polym14010132	2021
Enzymology	Characterization of a gene cluster encoding the maleylacetate reductase from Ralstonia eutropha 335T, an enzyme recruited for growth with 4-fluorobenzoate.	Seibert V, Thiel M, Hinner IS, Schlomann M.	Microbiology (Reading)	10.1099/mic.0.26602-0	2004
	Diversity of green-like and red-like ribulose-1,5-bisphosphate carboxylase/oxygenase large-subunit genes (cbbL) in differently managed agricultural soils.	Selesi D, Schmid M, Hartmann A.	Appl Environ Microbiol	10.1128/aem.71.1.175-184.2005	2005
	Alcaligenes eutrophus hydrogenase genes (Hox).	Hogrefe C, Romermann D, Friedrich B.	J Bacteriol	10.1128/jb.158.1.43-48.1984	1984
Metabolism	Naturally occurring genetic transfer of hydrogen-oxidizing ability between strains of Alcaligenes eutrophus.	Friedrich B, Hogrefe C, Schlegel HG.	J Bacteriol	10.1128/jb.147.1.198-205.1981	1981
Enzymology	Regulation of hydrogenase formation is temperature sensitive and plasmid coded in Alcaligenes eutrophus.	Friedrich CG, Friedrich B.	J Bacteriol	10.1128/jb.153.1.176-181.1983	1983
Metabolism	Enumeration and detection of anaerobic ferrous iron-oxidizing, nitrate-reducing bacteria from diverse European sediments.	Straub KL, Buchholz-Cleven BE.	Appl Environ Microbiol	10.1128/aem.64.12.4846-4856.1998	1998
Enzymology	Different types of dienelactone hydrolase in 4-fluorobenzoate-utilizing bacteria.	Schlomann M, Schmidt E, Knackmuss HJ.	J Bacteriol	10.1128/jb.172.9.5112-5118.1990	1990
	Identification and molecular characterization of the gene coding for acetaldehyde dehydrogenase II (acoD) of Alcaligenes eutrophus.	Priefert H, Kruger N, Jendrossek D, Schmidt B, Steinbuchel A.	J Bacteriol	10.1128/jb.174.3.899-907.1992	1992
	Cloning of the Alcaligenes eutrophus alcohol dehydrogenase gene.	Kuhn M, Jendrossek D, Frund C, Steinbuchel A, Schlegel HG.	J Bacteriol	10.1128/jb.170.2.685-692.1988	1988
	Production of polyhydroxyalkanoate (PHA) biopolymer from crop residue using bacteria as an alternative to plastics: a review.	Chouhan A, Tiwari A.	RSC Adv	10.1039/d4ra08505a	2025
	High-Yield Production of Polyhydroxybutyrate and Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from Crude Glycerol by a Newly Isolated Burkholderia Species Oh_219.	Oh SJ, Lim G, Han Y, Kim W, Joo HS, Kim YG, Kim JS, Bhatia SK, Yang YH.	Polymers (Basel)	10.3390/polym17020197	2025
	Polyhydroxyalkanoate production from rice straw hydrolysate obtained by alkaline pretreatment and enzymatic hydrolysis using Bacillus strains isolated from decomposing straw.	Van Thuoc D, Chung NT, Hatti-Kaul R.	Bioresour Bioprocess	10.1186/s40643-021-00454-7	2021
	Recent updates to microbial production and recovery of polyhydroxyalkanoates.	de Melo RN, de Souza Hassemer G, Steffens J, Junges A, Valduga E.	3 Biotech	10.1007/s13205-023-03633-9	2023
Phylogeny	Polyhydroxyalkanoates (PHAs) as Biomaterials in Tissue Engineering: Production, Isolation, Characterization.	Miu DM, Eremia MC, Moscovici M.	Materials (Basel)	10.3390/ma15041410	2022
Enzymology	Recent Trends in the Production and Recovery of Bioplastics Using Polyhydroxyalkanoates Copolymers.	Garcia A, Aguirre C, Perez A, Bahamonde SS, Urtuvia V, Diaz-Barrera A, Pena C.	Microorganisms	10.3390/microorganisms12112135	2024
	Quantitative 1H-NMR analysis reveals steric and electronic effects on the substrate specificity of benzoate dioxygenase in Ralstonia eutropha B9.	Bent JS, Clark ZT, Collins JA.	J Ind Microbiol Biotechnol	10.1093/jimb/kuac006	2022
Metabolism	Complete factorial design to adjust pH and sugar concentrations in the inoculum phase of Ralstonia solanacearum to optimize P(3HB) production.	Macagnan KL, Alves MI, Rodrigues AA, Furlan L, da Silva Rodrigues R, Diaz de Oliveira P, Vendruscolo CT, da Silveira Moreira A.	PLoS One	10.1371/journal.pone.0180563	2017
Metabolism	A benzene-degrading nitrate-reducing microbial consortium displays aerobic and anaerobic benzene degradation pathways.	Atashgahi S, Hornung B, van der Waals MJ, da Rocha UN, Hugenholtz F, Nijsse B, Molenaar D, van Spanning R, Stams AJM, Gerritse J, Smidt H.	Sci Rep	10.1038/s41598-018-22617-x	2018
Phylogeny	CDC group IV c-2: a new Ralstonia species close to Ralstonia eutropha.	Moissenet D, Goujon CP, Garbarg-Chenon A, Vu-Thien H.	J Clin Microbiol	10.1128/jcm.37.6.1777-1781.1999	1999
Metabolism	Bioenergetic strategy for the biodegradation of p-cresol by the unicellular green alga Scenedesmus obliquus.	Papazi A, Assimakopoulos K, Kotzabasis K.	PLoS One	10.1371/journal.pone.0051852	2012
Phylogeny	Phenotypic and genotypic characterization of clinical strains of CDC group IVc-2.	Osterhout GJ, Valentine JL, Dick JD.	J Clin Microbiol	10.1128/jcm.36.9.2618-2622.1998	1998
Metabolism	Ralstonia eutropha TF93 is blocked in tat-mediated protein export.	Bernhard M, Friedrich B, Siddiqui RA.	J Bacteriol	10.1128/jb.182.3.581-588.2000	2000
Enzymology	Phylogeny and functional expression of ribulose 1,5-bisphosphate carboxylase/oxygenase from the autotrophic ammonia-oxidizing bacterium Nitrosospira sp. isolate 40KI.	Utaker JB, Andersen K, Aakra A, Moen B, Nes IF.	J Bacteriol	10.1128/jb.184.2.468-478.2002	2002
	Construction and use of a gene bank of Alcaligenes eutrophus in the analysis of ribulose bisphosphate carboxylase genes.	Andersen K, Wilke-Douglas M.	J Bacteriol	10.1128/jb.159.3.973-978.1984	1984
Metabolism	Control of catechol meta-cleavage pathway in Alcaligenes eutrophus.	Hughes EJ, Bayly RC.	J Bacteriol	10.1128/jb.154.3.1363-1370.1983	1983
Metabolism	Acetate utilization is inhibited by benzoate in Alcaligenes eutrophus: evidence for transcriptional control of the expression of acoE coding for acetyl coenzyme A synthetase.	Ampe F, Lindley ND.	J Bacteriol	10.1128/jb.177.20.5826-5833.1995	1995
Metabolism	Benzoate degradation via the ortho pathway in Alcaligenes eutrophus is perturbed by succinate.	Ampe F, Uribelarrea JL, Aragao GM, Lindley ND.	Appl Environ Microbiol	10.1128/aem.63.7.2765-2770.1997	1997
Phylogeny	Deoxyribonucleic acid homologies of some so-called "Hydrogenomonas" species.	Ralston E, Palleroni NJ, Doudoroff M.	J Bacteriol	10.1128/jb.109.1.465-466.1972	1972
	Yields of Hydrogenomonas eutropha from growth on succinate and fumarate.	Bongers L.	J Bacteriol	10.1128/jb.102.2.598-599.1970	1970
Metabolism	Repression of phenol catabolism by organic acids in Ralstonia eutropha.	Ampe F, Leonard D, Lindley ND.	Appl Environ Microbiol	10.1128/aem.64.1.1-6.1998	1998
	Genetic and physical mapping and expression in Pseudomonas aeruginosa of the chromosomally encoded ribulose bisphosphate carboxylase genes of Alcaligenes eutrophus.	Andersen K, Wilke-Douglas M.	J Bacteriol	10.1128/jb.169.5.1997-2004.1987	1987
Metabolism	Extensive degradation of Aroclors and environmentally transformed polychlorinated biphenyls by Alcaligenes eutrophus H850.	Bedard DL, Wagner RE, Brennan MJ, Haberl ML, Brown JF.	Appl Environ Microbiol	10.1128/aem.53.5.1094-1102.1987	1987
Metabolism	Characterization of a TOL-like plasmid from Alcaligenes eutrophus that controls expression of a chromosomally encoded p-cresol pathway.	Hughes EJ, Bayly RC, Skurray RA.	J Bacteriol	10.1128/jb.158.1.73-78.1984	1984
Metabolism	Purification and properties of a protein linked to the soluble hydrogenase of hydrogen-oxidizing bacteria.	Karst U, Suetin S, Friedrich CG.	J Bacteriol	10.1128/jb.169.5.2079-2085.1987	1987
Metabolism	Dissimilation of aromatic compounds by Alcaligenes eutrophus.	Johnson BF, Stanier RY.	J Bacteriol	10.1128/jb.107.2.468-475.1971	1971
Metabolism	Energy generation and utilization in hydrogen bacteria.	Bongers L.	J Bacteriol	10.1128/jb.104.1.145-151.1970	1970
Metabolism	Regulation of the -ketoadipate pathway in Alcaligenes eutrophus.	Johnson BF, Stanier RY.	J Bacteriol	10.1128/jb.107.2.476-485.1971	1971
Proteome	The nutritional composition and cell size of microbial biomass for food applications are defined by the growth conditions.	Sakarika M, Kerckhof FM, Van Peteghem L, Pereira A, Van Den Bossche T, Bouwmeester R, Gabriels R, Van Haver D, Ulcar B, Martens L, Impens F, Boon N, Ganigue R, Rabaey K.	Microb Cell Fact	10.1186/s12934-023-02265-1	2023
	Raman Spectroscopy-Based Measurements of Single-Cell Phenotypic Diversity in Microbial Populations.	Garcia-Timermans C, Props R, Zacchetti B, Sakarika M, Delvigne F, Boon N.	mSphere	10.1128/msphere.00806-20	2020
	PHA Production and PHA Synthases of the Halophilic Bacterium Halomonas sp. SF2003.	Thomas T, Sudesh K, Bazire A, Elain A, Tan HT, Lim H, Bruzaud S.	Bioengineering (Basel)	10.3390/bioengineering7010029	2020
	Emergent Approaches to Efficient and Sustainable Polyhydroxyalkanoate Production.	Bedade DK, Edson CB, Gross RA.	Molecules	10.3390/molecules26113463	2021
Genetics	Comparative Genomic Assessment of the Cupriavidus necator Species for One-Carbon Based Biomanufacturing.	Jespersen MG, Vangsgaard EF, Saavedra MA, Donati S, Nielsen LK.	Microb Biotechnol	10.1111/1751-7915.70201	2025
Biotechnology	Development of Clostridium saccharoperbutylacetonicum as a Whole Cell Biocatalyst for Production of Chirally Pure (R)-1,3-Butanediol.	Grosse-Honebrink A, Little GT, Bean Z, Heldt D, Cornock RHM, Winzer K, Minton NP, Green E, Zhang Y.	Front Bioeng Biotechnol	10.3389/fbioe.2021.659895	2021
	Chemoenzymatic synthesis of 3-ethyl-2,5-dimethylpyrazine by L-threonine 3-dehydrogenase and 2-amino-3-ketobutyrate CoA ligase/L-threonine aldolase.	Motoyama T, Nakano S, Hasebe F, Miyata R, Kumazawa S, Miyoshi N, Ito S.	Commun Chem	10.1038/s42004-021-00545-8	2021
Enzymology	A novel A3 group aconitase tolerates oxidation and nitric oxide.	Doi Y, Takaya N.	J Biol Chem	10.1074/jbc.m114.614164	2015
Phylogeny	Complete Genome Sequence of 3-Chlorobenzoate-Degrading Bacterium Cupriavidus necator NH9 and Reclassification of the Strains of the Genera Cupriavidus and Ralstonia Based on Phylogenetic and Whole-Genome Sequence Analyses.	Moriuchi R, Dohra H, Kanesaki Y, Ogawa N.	Front Microbiol	10.3389/fmicb.2019.00133	2019
	Acetate excretion by a methanotroph, Methylocaldum marinum S8, under aerobic conditions.	Takeuchi M, Yoshioka H	Biosci Biotechnol Biochem	10.1093/bbb/zbab150	2021
Metabolism	PHA granule formation and degradation by Cupriavidus necator under different nutritional conditions.	Nygaard D, Yashchuk O, Hermida EB	J Basic Microbiol	10.1002/jobm.202100184	2021
Biotechnology	Improved fermentation strategies in a bioreactor for enhancing poly(3-hydroxybutyrate) (PHB) production by wild type Cupriavidus necator from fructose.	Nygaard D, Yashchuk O, Noseda DG, Araoz B, Hermida EB	Heliyon	10.1016/j.heliyon.2021.e05979	2021
Biotechnology	Evaluation of culture medium on poly(3-hydroxybutyrate) production by Cupriavidus necator ATCC 17697: application of the response surface methodology.	Nygaard D, Yashchuk O, Hermida EB	Heliyon	10.1016/j.heliyon.2019.e01374	2019
Metabolism	Biosynthesis of poly-3-hydroxybutyrate from grass silage by a two-stage fermentation process based on an integrated biorefinery concept.	Schwarz D, Schoenenwald AKJ, Dorrstein J, Sterba J, Kahoun D, Fojtikova P, Vilimek J, Schieder D, Zollfrank C, Sieber V	Bioresour Technol	10.1016/j.biortech.2018.08.064	2018
Enzymology	Highly selective L-threonine 3-dehydrogenase from Cupriavidus necator and its use in determination of L-threonine.	Ueatrongchit T, Asano Y	Anal Biochem	10.1016/j.ab.2010.11.003	2010

References

#129	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 531
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#41806	; Curators of the CIP;
#44416	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 1776
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#119146	Collection of Institut Pasteur ; Curators of the CIP; CIP 104763
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Wautersia eutropha (DSM 531, ATCC 17697, CCUG 1776)

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