Name: Burkholderia contaminans J2956
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1989

Type strain:

Species: Burkholderia contaminans

Strain Designation: J2956

Culture col. no.: DSM 22706, CCUG 55526, LMG 23361

Strain history: <- CCUG <- E. Vanlaere, LMG <- P. Vandamme, LMG <- J. Govan, Univ. Edinburgh, UK <- E. Berriatua

NCBI tax ID(s): 1334628 (strain), 488447 (species)

SI-ID 28924

CCUG 55526, LMG 23361, DSM 22706

Other strains from Burkholderia contaminans

B. contaminans CCUG 34411, LMG 16227
B. contaminans CCUG 54369
B. contaminans CCUG 58711
B. contaminans CCUG 58713
B. contaminans CCUG 59019
B. contaminans CCUG 59223
B. contaminans CCUG 59607

Section

Burkholderia contaminans J2956 is an aerobe, mesophilic, Gram-negative human pathogen that has a yellow pigmentation and was isolated from milk of a sheep with mastitis.

Gram-negative
pigmented
rod-shaped
aerobe
human pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Burkholderiaceae*
	Genus	*Burkholderia*
	Species	**Burkholderia contaminans**
	Full Scientific Name (LPSN)	Burkholderia contaminans Vanlaere et al. 2009

Information on morphological and physiological properties Morphology

[Ref.: #23366]	Gram stain	negative

[Ref.: #23366]	Cell shape	rod-shaped

[Ref.: #23366]

Colony color

metallic sheen

[Ref.: #23366]	Pigment production	yes
[Ref.: #23366]	Pigmentation color	yellow

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16476]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #16476]

Culture medium growth

[Ref.: #16476]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16476]

Culture medium composition

expand / minimize

[Ref.: #16476]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #16476]

Culture medium growth

[Ref.: #16476]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16476]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #16476]	growth	28 °C
[Ref.: #23366]	growth	30-42 °C
[Ref.: #60633]	growth	37 °C

[Ref.: #16476]	Temperature range	mesophilic
[Ref.: #60633]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23366]	Oxygen tolerance	aerobe
[Ref.: #60633]	Oxygen tolerance	aerobe

[Ref.: #23366]

Ability of spore formation

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23366]	adipate ChEBI	+	assimilation
[Ref.: #68369]	adipate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #23366]	citrate ChEBI	+	assimilation
[Ref.: #23366]	D-gluconate ChEBI	+	assimilation
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #23366]	D-mannitol ChEBI	+	assimilation
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #23366]	D-mannose ChEBI	+	assimilation
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #23366]	decanoate ChEBI	+	assimilation
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #23366]	esculin ChEBI	+	hydrolysis
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #23366]	glucose ChEBI	+	assimilation
[Ref.: #23366]	glucose ChEBI	+	builds acid from
[Ref.: #23366]	L-arabinose ChEBI	+	assimilation
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #23366]	L-malate ChEBI	+	assimilation
[Ref.: #23366]	lactose ChEBI	+	builds acid from
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #23366]	maltose ChEBI	-	assimilation
[Ref.: #23366]	maltose ChEBI	+	builds acid from
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #23366]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #23366]	nitrate ChEBI	+/-	reduction
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #23366]	phenylacetate ChEBI	+	assimilation
[Ref.: #23366]	ribitol ChEBI	+	builds acid from
[Ref.: #23366]	sucrose ChEBI	+	builds acid from
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #23366]	xylose ChEBI	+	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #23366]	arginine dihydrolase	-	3.5.3.6
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #23366]	beta-galactosidase	+	3.2.1.23
[Ref.: #23366]	beta-galactosidase	+/-	3.2.1.23
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE
[Ref.: #16476]	catalase	+	1.11.1.6
[Ref.: #23366]	cytochrome oxidase	+	1.9.3.1
[Ref.: #16476]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #68369]	gelatinase	+		* from API 20NE
[Ref.: #23366]	lysine decarboxylase	+	4.1.1.18
[Ref.: #23366]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #23366]	ornithine decarboxylase	+/-	4.1.1.17
[Ref.: #23366]	tryptophanase	-	4.1.99.1
[Ref.: #23366]	urease	-	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #16476]	24617	-	-	-	-	-	+	+	+	+	+	+	+	-	-	+	+	+	+	+	+	-
[Ref.: #16476]	23850	-	-	+	-	-	+	+	+	+	+	+	+	-	-	+	+	+	+	+	+	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16476]	Sample type/isolated from	milk of a sheep with mastitis
[Ref.: #16476]	Country	Spain
[Ref.: #16476]	Country ISO 3 Code	ESP
[Ref.: #16476]	Continent	Europe

[Ref.: #23366]	Sample type/isolated from	milk of a sheep with mastitis during an outbreak in 1999-2000

[Ref.: #60633]	Sample type/isolated from	Sheep milk,mastitis
[Ref.: #60633]	Country	Spain
[Ref.: #60633]	Country ISO 3 Code	ESP
[Ref.: #60633]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Infection	#Disease	-
#Host	#Mammals	#Caprinae (Sheep/Goat)
#Host Body Product	#Fluids	#Milk

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JX986975 (>99% sequence identity) for Burkholderia from Microbeatlas

Aquatic Samples	564
Soil Samples	1055
Animal Samples	1930
Plant Samples	1505
Total Samples	5054

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16476]	Pathogenicity (human)	yes
[Ref.: #16476]	Pathogenicity (animal)	yes
[Ref.: #16476]	Biosafety level	2	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16476]	Burkholderia sp. MS14 16S ribosomal RNA gene, partial sequence	EU938697	1455	ENA	557777 tax ID
[Ref.: #20218]	Burkholderia contaminans strain LMG 23361 16S ribosomal RNA gene, partial sequence	JX986975	1485	ENA	488447 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Burkholderia contaminans LMG 23361	GCA_000987075	contig	GenBank	1334628 tax ID	*
[Ref.: #66792]	Burkholderia contaminans LMG 23361	1334628.9	wgs	PATRIC	1334628 tax ID	*
[Ref.: #66792]	Burkholderia contaminans strain LMG 23361	488447.10	wgs	PATRIC	488447 tax ID	*
[Ref.: #66792]	Burkholderia contaminans LMG 23361	2681812996	draft	JGI IMG/M	1334628 tax ID	*
[Ref.: #66792]	Burkholderia contaminans LMG 23361	2916563313	draft	JGI IMG/M	1334628 tax ID	*
[Ref.: #66792]	Burkholderia contaminans LMG 23361	GCA_029633825	complete	GenBank	488447 tax ID	*

[Ref.: #16476]	GC-content	67 mol%
[Ref.: #23366]	GC-content	67.0 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Burkholderia contaminans LMG 23361 NCBI: GCA_029633825.1
[Ref.: #69480]	motile	yes	86.983	no
[Ref.: #69480]	flagellated	no	77.537	no
[Ref.: #69480]	gram-positive	no	97.762	yes
[Ref.: #69480]	anaerobic	no	98.517	yes
[Ref.: #69480]	aerobic	yes	92.2	no
[Ref.: #69480]	halophile	no	91.542	no
[Ref.: #69480]	spore-forming	no	91.835	no
[Ref.: #69480]	glucose-ferment	no	89.491	no
[Ref.: #69480]	thermophile	no	99.701	yes
[Ref.: #69480]	glucose-util	yes	89.902	yes

Availability in culture collections External links

[Ref.: #16476]

Culture collection no.

DSM 22706, CCUG 55526, LMG 23361

[Ref.: #71636]

SI-ID 28924

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Potential of Novel Sequence Type of Burkholderia cenocepacia for Biological Control of Root Rot of Maize (Zea mays L.) Caused by Fusarium temperatum.	Tagele SB, Kim SW, Lee HG, Lee YS	Int J Mol Sci	10.3390/ijms20051005	2019	*
Genetics	Improved High-Quality Draft Genome Sequence and Annotation of Burkholderia contaminans LMG 23361(T).	Jung JY, Ahn Y, Kweon O, LiPuma JJ, Hussong D, Marasa BS, Cerniglia CE	Genome Announc	10.1128/genomeA.00245-17	2017	*
Genetics	Draft Genome Sequences of Burkholderia contaminans, a Burkholderia cepacia Complex Species That Is Increasingly Recovered from Cystic Fibrosis Patients.	Bloodworth RA, Selin C, Lopez De Volder MA, Drevinek P, Galanternik L, Degrossi J, Cardona ST	Genome Announc	10.1128/genomeA.00766-15	2015	*
Phylogeny	Taxon K, a complex within the Burkholderia cepacia complex, comprises at least two novel species, Burkholderia contaminans sp. nov. and Burkholderia lata sp. nov.	Vanlaere E, Baldwin A, Gevers D, Henry D, De Brandt E, LiPuma JJ, Mahenthiralingam E, Speert DP, Dowson C, Vandamme P	Int J Syst Evol Microbiol	10.1099/ijs.0.001123-0	2009	*

References

#16476

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22706

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23366

Elke Vanlaere, Adam Baldwin, Dirk Gevers, Deborah Henry, Evie De Brandt, John J. LiPuma, Eshwar Mahenthiralingam, David P. Speert, Chris Dowson, Peter Vandamme: Taxon K, a complex within the Burkholderia cepacia complex, comprises at least two novel species, Burkholderia contaminans sp. nov. and Burkholderia lata sp. nov.. IJSEM 59: 102 - 111 2009 ( DOI 10.1099/ijs.0.001123-0 , PubMed 19126732 )

#60633 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 55526

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#71636

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID28924.1 )

* These data were automatically processed and therefore are not curated

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Burkholderia contaminans LMG 23361

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