Burkholderia seminalis (DSM 23518, CCUG 54564, LMG 24067)

Name: Burkholderia seminalis (DSM 23518, CCUG 54564, LMG 24067)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1987

Type strain

Strain Designation AU0475

Culture col. no. DSM 23518 CCUG 54564 LMG 24067 AU0475

NCBI tax ID(s) 1108969 488731

Special Collections DSMZ CCUG Literature strains

History <- CCUG; CCUG 54564 <- E. Vanlaere, LMG; LMG 24067 <- J. J. LiPuma; AU0475

Links

Burkholderia seminalis AU0475 is an aerobe, mesophilic, Gram-negative prokaryote that has a yellow pigmentation and was isolated from human sputum from cystic fibrosis patient.

Gram-negative rod-shaped pigmented aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 137158

LMG 24067,CCUG 54564,DSM 23518

@ref 20215

Last LPSN update 2026-02-09 11:18:13

Domain Bacteria

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Burkholderiaceae

Genus Burkholderia

Species Burkholderia seminalis

Full scientific name Burkholderia seminalis Vanlaere et al. 2008

BacDive ID	Other strains from **Burkholderia seminalis** (2)	Type strain
154607	B. seminalis CCUG 54565, LMG 24272
154608	B. seminalis CCUG 54566, LMG 24273

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
23365	negative	rod-shaped
125439	negative		96.2
125438	negative		97.969

Colony morphology

17254

Type of hemolysisgamma

Pigmentation

@ref	Production	Color
23365		yellow

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
17254	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)		Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
17254	R2A MEDIUM (DSMZ Medium 830)		Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
17254	positive	growth	30	mesophilic
23365	positive	growth	30-37	mesophilic
23365	inconsistent	growth	42
60238	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
23365	aerobe
60238	aerobe
125438	aerobe	90.395
125439	obligate aerobe	99.2

Spore formation

@ref	Spore formation	Confidence
23365
125439		97.3

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23365	17128 ChEBI	adipate	+	assimilation
68369	17128 ChEBI	adipate	+	assimilation	from API 20NE
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
23365	16947 ChEBI	citrate	+	assimilation
23365	8391 ChEBI	D-gluconate	+	assimilation
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	+	fermentation	from API 20NE
23365	16899 ChEBI	D-mannitol	+	assimilation
68369	16899 ChEBI	D-mannitol	+	assimilation	from API 20NE
23365	16024 ChEBI	D-mannose	+	assimilation
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
23365	27689 ChEBI	decanoate	+	assimilation
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
23365	4853 ChEBI	esculin	+	hydrolysis
68369	4853 ChEBI	esculin	+	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	+	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
23365	17234 ChEBI	glucose	+	assimilation
23365	17234 ChEBI	glucose	+	builds acid from
23365	30849 ChEBI	L-arabinose	+	assimilation
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
23365	15589 ChEBI	L-malate	+	assimilation
23365	17716 ChEBI	lactose	+	builds acid from
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
23365	17306 ChEBI	maltose	-	assimilation
23365	17306 ChEBI	maltose	+	builds acid from
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
23365	506227 ChEBI	N-acetylglucosamine	+	assimilation
68369	59640 ChEBI	N-acetylglucosamine	+	assimilation	from API 20NE
23365	17632 ChEBI	nitrate	-	reduction
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
23365	18401 ChEBI	phenylacetate	+	assimilation
23365	15963 ChEBI	ribitol	+	builds acid from
23365	17992 ChEBI	sucrose	+	assimilation
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE
23365	18222 ChEBI	xylose	+	builds acid from

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
23365	arginine dihydrolase	-	3.5.3.6
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
23365	beta-galactosidase	+	3.2.1.23
68369	beta-glucosidase	+	3.2.1.21	from API 20NE
17254	catalase	+	1.11.1.6
23365	cytochrome oxidase	+	1.9.3.1
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
17254	cytochrome-c oxidase	+	1.9.3.1
23365	gelatinase	+
68369	gelatinase	+		from API 20NE
23365	lysine decarboxylase	+	4.1.1.18
23365	ornithine decarboxylase	+	4.1.1.17
23365	tryptophanase	-	4.1.99.1
23365	urease	-	3.5.1.5
68369	urease	-	3.5.1.5	from API 20NE

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
17254	-	-	+	-	-	+	+	+	+	+	+	+	+	-	+	+	+	+	+	+	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Infection	#Disease	#Cystic fibrosis
#Infection	#Patient	-
#Host	#Human	-
#Host Body Product	#Fluids	#Sputum

Isolation

@ref	Sample type	Sampling date	Country	Country ISO 3 Code	Continent
17254	human sputum from cystic fibrosis patient		USA	USA	North America
23365		1998
60238	Human sputum,cystic fibrosis	2004-06-03	USA	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence AM747631 (>99% sequence identity) for Burkholderia from Microbeatlas

Aquatic Samples	564
Soil Samples	1055
Animal Samples	1930
Plant Samples	1505
Total Samples	5054

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
17254	2	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	Burkholderia seminalis LMG 24067 assembly for Burkholderia seminalis DSM 23518 LMG 24067	scaffold	GCA_902832885	1108969.3	8115411739	1108969	67.45
66792	ASM90249916v1 assembly for Burkholderia seminalis	contig	GCA_902499165	488731.17	8115383368	488731	40

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
17254	Burkholderia seminalis DSM 2351 partial 16S rRNA gene, type strain R-24196T	AM747631	1498		1108969

GC content

23365

GC-content (mol%)67.0

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Burkholderia seminalis DSM 23518 LMG 24067
NCBI: GCA_902832885

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.20	no
125439	gram_stain	BacteriaNetⓘ	negative	96.20	no
125439	motility	BacteriaNetⓘ	yes	78.40	no
125439	spore_formation	BacteriaNetⓘ	no	97.30	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Burkholderia seminalis DSM 23518 LMG 24067
NCBI: GCA_902832885.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	97.97	yes
125438	anaerobic	anaerobicⓘ	no	96.66	yes
125438	aerobic	aerobicⓘ	yes	90.40	yes
125438	spore-forming	spore-formingⓘ	no	84.41	yes
125438	thermophilic	thermophileⓘ	no	98.00	yes
125438	flagellated	motile2+ⓘ	yes	84.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Multi-omics analysis of niche specificity provides new insights into ecological adaptation in bacteria.	Zhu B, Ibrahim M, Cui Z, Xie G, Jin G, Kube M, Li B, Zhou X.	ISME J	10.1038/ismej.2015.251	2016
	Genomic Assemblies of Members of Burkholderia and Related Genera as a Resource for Natural Product Discovery.	Mullins AJ, Jones C, Bull MJ, Webster G, Parkhill J, Connor TR, Murray JAH, Challis GL, Mahenthiralingam E.	Microbiol Resour Announc	10.1128/mra.00485-20	2020
	Effect of beta-Lactamase inhibitors on in vitro activity of beta-Lactam antibiotics against Burkholderia cepacia complex species.	Everaert A, Coenye T.	Antimicrob Resist Infect Control	10.1186/s13756-016-0142-3	2016
Pathogenicity	Investigating the Role of the Host Multidrug Resistance Associated Protein Transporter Family in Burkholderia cepacia Complex Pathogenicity Using a Caenorhabditis elegans Infection Model.	Tedesco P, Visone M, Parrilli E, Tutino ML, Perrin E, Maida I, Fani R, Ballestriero F, Santos R, Pinilla C, Di Schiavi E, Tegos G, de Pascale D.	PLoS One	10.1371/journal.pone.0142883	2015
Phylogeny	Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex.	Vanlaere E, Lipuma JJ, Baldwin A, Henry D, De Brandt E, Mahenthiralingam E, Speert D, Dowson C, Vandamme P	Int J Syst Evol Microbiol	10.1099/ijs.0.65634-0	2008

References

#17254	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23518
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#23365	Elke Vanlaere, John J. LiPuma, Adam Baldwin, Deborah Henry, Evie De Brandt, Eshwar Mahenthiralingam, David Speert, Chris Dowson, Peter Vandamme: Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex. IJSEM 58: 1580 - 1590 2008 ( DOI 10.1099/ijs.0.65634-0 , PubMed 18599699 )
#60238	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 54564
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68369	Automatically annotated from API 20NE .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Burkholderia seminalis (DSM 23518, CCUG 54564, LMG 24067)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_902832885

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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