Name: Burkholderia seminalis AU0475
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1987

Type strain:

Species: Burkholderia seminalis

Strain Designation: AU0475

Culture col. no.: DSM 23518, CCUG 54564, LMG 24067

Strain history: <- CCUG <- E. Vanlaere, LMG <- J. J. LiPuma; AU0475

NCBI tax ID(s): 1108969 (strain), 488731 (species)

Other strains from Burkholderia seminalis

B. seminalis CCUG 54565, LMG 24272
B. seminalis CCUG 54566, LMG 24273

Section

Burkholderia seminalis AU0475 is an aerobe, mesophilic, Gram-negative bacterium that has a yellow pigmentation and was isolated from human sputum from cystic fibrosis patient.

Gram-negative
pigmented
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Burkholderiaceae*
	Genus	*Burkholderia*
	Species	**Burkholderia seminalis**
	Full Scientific Name (PNU)	Burkholderia seminalis Vanlaere et al. 2008

Information on morphological and physiological properties Morphology

[Ref.: #23365]	Gram stain	negative
[Ref.: #23365]	Cell shape	rod-shaped

[Ref.: #17254]

Type of hemolysis

gamma

[Ref.: #23365]	Pigment production	yes
[Ref.: #23365]	Pigmentation color	yellow

Information on culture and growth conditions Culture and growth conditions

[Ref.: #17254]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #17254]

Culture medium growth

[Ref.: #17254]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #17254]

Culture medium composition

expand / minimize

[Ref.: #17254]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #17254]

Culture medium growth

[Ref.: #17254]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #17254]

Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #17254]		growth	30 °C
[Ref.: #23365]		growth	30-37 °C
[Ref.: #23365]	inconsistent	growth	42 °C
[Ref.: #60238]		growth	37 °C

[Ref.: #17254]	Temperature range	mesophilic
[Ref.: #23365]	Temperature range	mesophilic
[Ref.: #23365]	Temperature range	thermophilic
[Ref.: #60238]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23365]	Oxygen tolerance	aerobe
[Ref.: #60238]	Oxygen tolerance	aerobe

[Ref.: #23365]

Ability of spore formation

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23365]	adipate ChEBI	+	assimilation
[Ref.: #68369]	adipate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #23365]	citrate ChEBI	+	assimilation
[Ref.: #23365]	D-gluconate ChEBI	+	assimilation
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	fermentation	* from API 20NE
[Ref.: #23365]	D-mannitol ChEBI	+	assimilation
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #23365]	D-mannose ChEBI	+	assimilation
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #23365]	decanoate ChEBI	+	assimilation
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #23365]	esculin ChEBI	+	hydrolysis
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #23365]	glucose ChEBI	+	assimilation
[Ref.: #23365]	glucose ChEBI	+	builds acid from
[Ref.: #23365]	L-arabinose ChEBI	+	assimilation
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #23365]	L-malate ChEBI	+	assimilation
[Ref.: #23365]	lactose ChEBI	+	builds acid from
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #23365]	maltose ChEBI	-	assimilation
[Ref.: #23365]	maltose ChEBI	+	builds acid from
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #23365]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #23365]	nitrate ChEBI	-	reduction
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #23365]	phenylacetate ChEBI	+	assimilation
[Ref.: #23365]	ribitol ChEBI	+	builds acid from
[Ref.: #23365]	sucrose ChEBI	+	assimilation
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #23365]	xylose ChEBI	+	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #68369]		indole ChEBI	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #23365]	arginine dihydrolase	-	3.5.3.6
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #23365]	beta-galactosidase	+	3.2.1.23
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE
[Ref.: #17254]	catalase	+	1.11.1.6
[Ref.: #23365]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #17254]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #23365]	gelatinase	+
[Ref.: #68369]	gelatinase	+		* from API 20NE
[Ref.: #23365]	lysine decarboxylase	+	4.1.1.18
[Ref.: #23365]	ornithine decarboxylase	+	4.1.1.17
[Ref.: #23365]	tryptophanase	-	4.1.99.1
[Ref.: #23365]	urease	-	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #17254]	21256	-	-	+	-	-	+	+	+	+	+	+	+	+	-	+	+	+	+	+	+	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #17254]	Sample type/isolated from	human sputum from cystic fibrosis patient
[Ref.: #17254]	Country	USA
[Ref.: #17254]	Country ISO 3 Code	USA
[Ref.: #17254]	Continent	North America

[Ref.: #60238]	Sample type/isolated from	Human sputum,cystic fibrosis
[Ref.: #60238]	Sampling date	2004-06-03
[Ref.: #60238]	Country	USA
[Ref.: #60238]	Country ISO 3 Code	USA
[Ref.: #60238]	Continent	North America

[Ref.: #23365]	Sampling date	1998
* marker position based on {}

Isolation sources categories

#Infection	#Disease	#Cystic fibrosis
#Infection	#Patient	-
#Host	#Human	-
#Host Body Product	#Fluids	#Sputum

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AM747631 (>99% sequence identity) for Burkholderia from Microbeatlas

Aquatic Samples	564
Soil Samples	1055
Animal Samples	1930
Plant Samples	1505
Total Samples	5054

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #17254]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #17254]	Burkholderia seminalis DSM 2351 partial 16S rRNA gene, type strain R-24196T	AM747631	1498	ENA	1108969 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Burkholderia seminalis LMG 24067	GCA_902499165	contig	GenBank	488731 tax ID	*
[Ref.: #66792]	Burkholderia seminalis DSM 23518 LMG 24067	GCA_902832885	scaffold	GenBank	1108969 tax ID	*
[Ref.: #66792]	Burkholderia seminalis DSM 23518 strain LMG 24067	1108969.3	wgs	PATRIC	1108969 tax ID	*
[Ref.: #66792]	Burkholderia seminalis strain LMG 24067	488731.17	wgs	PATRIC	488731 tax ID	*
[Ref.: #66792]	Tetragenococcus halophilus TMW 2.2264	GCA_024137075	contig	PATRIC	51669 tax ID	*
[Ref.: #66792]	Parabacteroides goldsteinii A19_11.PG.DSS	GCA_910574435	scaffold	PATRIC	328812 tax ID	*

[Ref.: #23365]

GC-content

67.0 mol%

Availability in culture collections External links

[Ref.: #17254]

Culture collection no.

DSM 23518, CCUG 54564, LMG 24067

[Ref.: #20218]

Associated Passport(s) in StrainInfo

719691, 719434

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex.	Vanlaere E, Lipuma JJ, Baldwin A, Henry D, De Brandt E, Mahenthiralingam E, Speert D, Dowson C, Vandamme P	Int J Syst Evol Microbiol	10.1099/ijs.0.65634-0	2008	*

References

#17254

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23518

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23365

Elke Vanlaere, John J. LiPuma, Adam Baldwin, Deborah Henry, Evie De Brandt, Eshwar Mahenthiralingam, David Speert, Chris Dowson, Peter Vandamme: Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex. IJSEM 58: 1580 - 1590 2008 ( DOI 10.1099/ijs.0.65634-0 , PubMed 18599699 )

#60238 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 54564

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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