Burkholderia metallica (DSM 23519, CCUG 54567, LMG 24068)

Name: Burkholderia metallica (DSM 23519, CCUG 54567, LMG 24068)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1986

Type strain

Strain Designation AU0553

Culture col. no. DSM 23519 CCUG 54567 LMG 24068 AU0553

NCBI tax ID(s) 488729

Special Collections DSMZ CCUG Literature strains

History DSMZ <- CCUG <- E. Vanlaere, LMG <- J. J. LiPuma; AU0553

Links

Burkholderia metallica AU0553 is an aerobe, Gram-negative, rod-shaped bacterium that has a yellow pigmentation and was isolated from human sputum from cystic fibrosis patient.

Gram-negative rod-shaped pigmented aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 95222

LMG 24068,CCUG 54567,DSM 23519

@ref 20215

Last LPSN update 2026-05-29 08:25:23

Domain Bacteria

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Burkholderiaceae

Genus Burkholderia

Species Burkholderia metallica

Full scientific name Burkholderia metallica Vanlaere et al. 2008

BacDive ID	Other strains from **Burkholderia metallica** (2)	Type strain
154609	B. metallica CCUG 54568, LMG 24274
154610	B. metallica CCUG 54569, LMG 24275

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
23365	negative	rod-shaped
125438	negative		98

Colony morphology

@ref	Type of hemolysis	Hemolysis ability	Colony color
17255	gamma	0
23365			metallic shine

Pigmentation

@ref	Production	Color
23365		yellow

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
17255	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)		Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
17255	R2A MEDIUM (DSMZ Medium 830)		Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
17255	positive	growth	30
23365	positive	growth	30-42
60241	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
23365	aerobe
60241	aerobe
125438	aerobe	91.579
125439	obligate aerobe	99.228

Spore formation

@ref	Spore formation	Confidence
23365
125439		98.364

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23365	17128 ChEBI	adipate	+	assimilation
68369	17128 ChEBI	adipate	+	assimilation	from API 20NE
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
23365	16947 ChEBI	citrate	+	growth
23365	8391 ChEBI	D-gluconate	+	growth
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
23365	16899 ChEBI	D-mannitol	+	assimilation
68369	16899 ChEBI	D-mannitol	+	assimilation	from API 20NE
23365	16024 ChEBI	D-mannose	+	growth
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
23365	27689 ChEBI	decanoate	+	assimilation
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
23365	4853 ChEBI	esculin	+	hydrolysis
68369	4853 ChEBI	esculin	+	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	+	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
23365	17234 ChEBI	glucose	+	builds acid from
23365	17234 ChEBI	glucose	+	growth
23365	30849 ChEBI	L-arabinose	+	assimilation
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
23365	15589 ChEBI	L-malate	+	growth
23365	17716 ChEBI	lactose	+	builds acid from
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
23365	17306 ChEBI	maltose	-	assimilation
23365	17306 ChEBI	maltose	+	builds acid from
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
23365	506227 ChEBI	N-acetylglucosamine	+	assimilation
68369	59640 ChEBI	N-acetylglucosamine	+	assimilation	from API 20NE
23365	17632 ChEBI	nitrate	-	reduction
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
23365	18401 ChEBI	phenylacetate	+	assimilation
23365	15963 ChEBI	ribitol	+	builds acid from
23365	17992 ChEBI	sucrose	+	builds acid from
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE
23365	18222 ChEBI	xylose	+	builds acid from

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
23365	arginine dihydrolase	-	3.5.3.6
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
23365	beta-galactosidase	+	3.2.1.23
68369	beta-glucosidase	+	3.2.1.21	from API 20NE
17255	catalase	+	1.11.1.6
23365	cytochrome oxidase	+	1.9.3.1
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
17255	cytochrome-c oxidase	+	1.9.3.1
23365	gelatinase	+
68369	gelatinase	+		from API 20NE
23365	lysine decarboxylase	+	4.1.1.18
23365	ornithine decarboxylase	-	4.1.1.17
23365	tryptophanase	-	4.1.99.1
23365	urease	-	3.5.1.5
68369	urease	-	3.5.1.5	from API 20NE

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
17255	-	-	+/-	-	-	+	+	+	+	+	+	+	+	-	+	+	+	+	+	+	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Infection	#Disease	#Cystic fibrosis
#Infection	#Patient	-
#Host	#Human	-
#Host Body Product	#Fluids	#Sputum

Isolation

@ref	Sample type	Host species	Sampling date	Country	Country ISO 3 Code	Continent
17255	human sputum from cystic fibrosis patient	Homo sapiens		USA	USA	North America
23365			1998
60241	Human sputum,cystic fibrosis	Homo sapiens	2001-09-07	USA	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence AM747632 (>99% sequence identity) for Burkholderia from Microbeatlas

Aquatic Samples	564
Soil Samples	1055
Animal Samples	1930
Plant Samples	1505
Total Samples	5054

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
17255	2	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM90283284v1 assembly for Burkholderia metallica LMG 24068	scaffold	GCA_902832845	488729.16	8115404775	488729	72.32
66792	ASM90249906v1 assembly for Burkholderia metallica	contig	GCA_902499065	488729.11	8113316241	488729	56.32

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
17255	Burkholderia metallica partial 16S rRNA gene, type strain R-16017T	AM747632	1505		488729

GC content

23365

GC-content (mol%)67.0

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM90283284v1 assembly for Burkholderia metallica LMG 24068
BV-BRC: 488729.16

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.23	no
125439	gram_stain	BacteriaNetⓘ	negative	77.89	no
125439	motility	BacteriaNetⓘ	yes	62.74	no
125439	spore_formation	BacteriaNetⓘ	no	98.36	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Burkholderia metallica LMG 24068
NCBI: GCA_902832845.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.00	yes
125438	anaerobic	anaerobicⓘ	no	97.68	yes
125438	aerobic	aerobicⓘ	yes	91.58	no
125438	spore-forming	spore-formingⓘ	no	87.16	no
125438	thermophilic	thermophileⓘ	no	98.00	yes
125438	flagellated	motile2+ⓘ	yes	84.81	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Phylogeny, Synteny, and Distribution of Type III Secretion Systems in Burkholderia cenocepacia: A Closer Look Into Host Span.	Cordeiro GT, de Oliveira HL, Mariano DCO, Torres YS, Dias GM, Neves BC.	Microbiologyopen	10.1002/mbo3.70101	2025
	Evaluation of Burkholderia cepacia Complex Bacteria Pathogenicity Using Caenorhabditis elegans.	Tedesco P, Di Schiavi E, Esposito FP, de Pascale D.	Bio Protoc	10.21769/bioprotoc.1964	2016
	Genomic Assemblies of Members of Burkholderia and Related Genera as a Resource for Natural Product Discovery.	Mullins AJ, Jones C, Bull MJ, Webster G, Parkhill J, Connor TR, Murray JAH, Challis GL, Mahenthiralingam E.	Microbiol Resour Announc	10.1128/mra.00485-20	2020
Genetics	Isolation and Characterization of Strain Exiguobacterium sp. KRL4, a Producer of Bioactive Secondary Metabolites from a Tibetan Glacier.	Tedesco P, Palma Esposito F, Masino A, Vitale GA, Tortorella E, Poli A, Nicolaus B, van Zyl LJ, Trindade M, de Pascale D.	Microorganisms	10.3390/microorganisms9050890	2021
	Characterization of a New Mixture of Mono-Rhamnolipids Produced by Pseudomonas gessardii Isolated from Edmonson Point (Antarctica).	Buonocore C, Tedesco P, Vitale GA, Esposito FP, Giugliano R, Monti MC, D'Auria MV, de Pascale D.	Mar Drugs	10.3390/md18050269	2020
	Effect of beta-Lactamase inhibitors on in vitro activity of beta-Lactam antibiotics against Burkholderia cepacia complex species.	Everaert A, Coenye T.	Antimicrob Resist Infect Control	10.1186/s13756-016-0142-3	2016
Pathogenicity	Investigating the Role of the Host Multidrug Resistance Associated Protein Transporter Family in Burkholderia cepacia Complex Pathogenicity Using a Caenorhabditis elegans Infection Model.	Tedesco P, Visone M, Parrilli E, Tutino ML, Perrin E, Maida I, Fani R, Ballestriero F, Santos R, Pinilla C, Di Schiavi E, Tegos G, de Pascale D.	PLoS One	10.1371/journal.pone.0142883	2015
Phylogeny	Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex.	Vanlaere E, Lipuma JJ, Baldwin A, Henry D, De Brandt E, Mahenthiralingam E, Speert D, Dowson C, Vandamme P	Int J Syst Evol Microbiol	10.1099/ijs.0.65634-0	2008

References

#17255	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23519
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#23365	Elke Vanlaere, John J. LiPuma, Adam Baldwin, Deborah Henry, Evie De Brandt, Eshwar Mahenthiralingam, David Speert, Chris Dowson, Peter Vandamme: Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex. IJSEM 58: 1580 - 1590 2008 ( DOI 10.1099/ijs.0.65634-0 , PubMed 18599699 )
#60241	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 54567
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68369	Automatically annotated from API 20NE .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Burkholderia metallica (DSM 23519, CCUG 54567, LMG 24068)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_902832845

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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