Name: Paraburkholderia mimosarum PAS44
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1972

Type strain:

Species: Paraburkholderia mimosarum

Strain Designation: PAS44

Culture col. no.: DSM 21841, BCRC 17516, CCUG 54296, LMG 23256

Strain history: <- LMG <- P. Vandamme, Univ. Ghent, Belgium <- W.-M. Chen, Dept. Seafood Science, Nat. Kaohsiung Marine Univ. Taiwan, China

NCBI tax ID(s): 1038869 (strain), 312026 (species)

SI-ID 289546

LMG 23256, BCRC 17516, CCUG 54296, CCRC 17516, NBRC 106338, DSM 21841

Section

Paraburkholderia mimosarum PAS44 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from root nodule of Mimosa pigra.

Gram-negative
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Burkholderiaceae*
	Genus	*Paraburkholderia*
	Species	**Paraburkholderia mimosarum**
	Full Scientific Name (LPSN)	Paraburkholderia mimosarum (Chen et al. 2006) Sawana et al. 2015

Synonym

Burkholderia mimosarum

Information on morphological and physiological properties Morphology

[Ref.: #31805]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.956

[Ref.: #31805]	Cell length	0.8-2 µm

[Ref.: #31805]	Cell width	0.5-0.7 µm

[Ref.: #31805]	Cell shape	rod-shaped

[Ref.: #69480]	Motility	yes Confidence in %92.191

[Ref.: #15984]	Incubation period	2-3 days

[Ref.: #60128]	Incubation period	2 days

[Ref.: #31805]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15984]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #15984]

Culture medium growth

[Ref.: #15984]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15984]

Culture medium composition

expand / minimize

[Ref.: #15984]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #15984]

Culture medium growth

[Ref.: #15984]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15984]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #15984]	growth	30 °C
[Ref.: #31805]	growth	28-37 °C
[Ref.: #31805]	optimum	28-37 °C
[Ref.: #60128]	growth	30 °C

[Ref.: #15984]	Temperature range	mesophilic
[Ref.: #31805]	Temperature range	mesophilic
[Ref.: #60128]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31805]			optimum	7
[Ref.: #31805]			growth	7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31805]	Oxygen tolerance	aerobe
[Ref.: #60128]	Oxygen tolerance	aerobe

[Ref.: #31805]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %99
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.974

[Ref.: #31805]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #31805]	arabinose ChEBI	+	carbon source
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #31805]	glucose ChEBI	+	carbon source
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #31805]	malate ChEBI	+	carbon source
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #31805]	mannitol ChEBI	+	carbon source
[Ref.: #31805]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	+	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	yes	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	+	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #31805]	catalase	+	1.11.1.6
[Ref.: #31805]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68369]	cytochrome oxidase	-	1.9.3.1	* from API 20NE
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #15984]	23834	+	+	-	-	-	-	-	+	+	+	+	+	+	-	+	+	-	+	+/-	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15984]	Sample type/isolated from	root nodule of Mimosa pigra
[Ref.: #15984]	Host species	Mimosa pigra
[Ref.: #15984]	Geographic location (country and/or sea, region)	Taiwan
[Ref.: #15984]	Country ISO 3 Code	CHN

[Ref.: #60128]	Sample type/isolated from	Root nodules of Mimosa pigra
[Ref.: #60128]	Geographic location (country and/or sea, region)	Anso
[Ref.: #60128]	Country	Taiwan
[Ref.: #60128]	Country ISO 3 Code	TWN
[Ref.: #60128]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Shrub (Scrub)
#Host Body-Site	#Plant	#Root nodule

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence HQ849089 (>99% sequence identity) for Paraburkholderia mimosarum subclade from Microbeatlas

Aquatic Samples	57
Soil Samples	535
Animal Samples	152
Plant Samples	145
Total Samples	889

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15984]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15984]	Burkholderia mimosarum strain PAS44 16S ribosomal RNA gene, partial sequence	AY752958	1439	ENA	312026 tax ID
[Ref.: #20218]	Burkholderia mimosarum strain LMG 23256 16S ribosomal RNA gene, partial sequence	HQ849089	1124	ENA	312026 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Paraburkholderia mimosarum LMG 23256 = NBRC 106338	GCA_000472825	scaffold	GenBank	1038869 tax ID	*
[Ref.: #66792]	Paraburkholderia mimosarum LMG 23256 = NBRC 106338	GCA_000739815	contig	GenBank	1038869 tax ID	*
[Ref.: #66792]	Paraburkholderia mimosarum LMG 23256	GCA_902833725	scaffold	GenBank	312026 tax ID	*
[Ref.: #66792]	Burkholderia mimosarum LMG 23256	1038869.3	wgs	PATRIC	1038869 tax ID	*
[Ref.: #66792]	Paraburkholderia mimosarum strain LMG 23256	312026.4	wgs	PATRIC	312026 tax ID	*
[Ref.: #66792]	Paraburkholderia mimosarum LMG 23256	2513237083	draft	JGI IMG/M	1038869 tax ID	*

[Ref.: #31805]

GC-content

63.8-64.8 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Paraburkholderia mimosarum LMG 23256 = NBRC 106338 LMG 23256 NCBI: GCA_000472825.1
[Ref.: #69481]	spore-forming	no	99	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Paraburkholderia mimosarum LMG 23256 = NBRC 106338 LMG 23256 NCBI: GCA_000472825.1
[Ref.: #69480]	motile	yes	83.108	no
[Ref.: #69480]	flagellated	no	82.061	no
[Ref.: #69480]	gram-positive	no	98.22	no
[Ref.: #69480]	anaerobic	no	98.238	no
[Ref.: #69480]	aerobic	yes	87.28	yes
[Ref.: #69480]	halophile	no	95.26	no
[Ref.: #69480]	spore-forming	no	93.666	no
[Ref.: #69480]	thermophile	no	99.509	yes
[Ref.: #69480]	glucose-util	yes	88.899	yes
[Ref.: #69480]	glucose-ferment	no	90.081	no

Availability in culture collections External links

[Ref.: #15984]

Culture collection no.

DSM 21841, BCRC 17516, CCUG 54296, LMG 23256

[Ref.: #71617]

SI-ID 289546

Literature:

	Title	Authors	Journal	DOI	Year
Genetics	Genome sequence of Burkholderia mimosarum strain LMG 23256(T), a Mimosa pigra microsymbiont from Anso, Taiwan.	Willems A, Tian R, Brau L, Goodwin L, Han J, Liolios K, Huntemann M, Pati A, Woyke T, Mavrommatis K, Markowitz V, Ivanova N, Kyrpides N, Reeve W	Stand Genomic Sci	10.4056/sigs.4848627	2013	*
Phylogeny	Burkholderia spp. are the most competitive symbionts of Mimosa, particularly under N-limited conditions.	Elliott GN, Chou JH, Chen WM, Bloemberg GV, Bontemps C, Martinez-Romero E, Velazquez E, Young JP, Sprent JI, James EK	Environ Microbiol	10.1111/j.1462-2920.2008.01799.x	2008	*
Phylogeny	Burkholderia mimosarum sp. nov., isolated from root nodules of Mimosa spp. from Taiwan and South America.	Chen WM, James EK, Coenye T, Chou JH, Barrios E, de Faria SM, Elliott GN, Sheu SY, Sprent JI, Vandamme P	Int J Syst Evol Microbiol	10.1099/ijs.0.64325-0	2006	*
	A Third Class: Functional Gibberellin Biosynthetic Operon in Beta-Proteobacteria.	Nagel R, Bieber JE, Schmidt-Dannert MG, Nett RS, Peters RJ	Front Microbiol	10.3389/fmicb.2018.02916	2018	*

References

#15984

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21841

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#31805

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28074 (see below)

#60128 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 54296

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#71617

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID289546.1 )

#28074

IJSEM 1847 2006 ( DOI 10.1099/ijs.0.64325-0 , PubMed 16902019 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Paraburkholderia mimosarum in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Paraburkholderia mimosarum LMG 23256 = NBRC 106338 LMG 23256

Paraburkholderia mimosarum LMG 23256 = NBRC 106338 LMG 23256

Change proposal

Change proposal