Name: Paraburkholderia phenoliruptrix AC1100
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1967

Type strain:

Species: Paraburkholderia phenoliruptrix

Strain Designation: AC1100

Culture col. no.: DSM 17773, CCUG 48558, CIP 108591, LMG 22037

Strain history: CIP <- 2004, CCUG <- 2003, LMG <- 2003, T. Coenye, Ghent Univ., Ghent, Belgium <- 1999, D. Henry, British Columbia Univ., Vancouver, Canada: strain AC1100

NCBI tax ID(s): 252970 (species)

SI-ID 43466

ATCC 53867, ATCC 39027, LMG22037, CCUG 48558, CIP 108591, DSM 17773

Section

Paraburkholderia phenoliruptrix AC1100 is an aerobe, mesophilic, motile bacterium that was isolated from chemostat with 2,4,5-trichlorophenoxyacetic acid.

motile
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Burkholderiaceae*
	Genus	*Paraburkholderia*
	Species	**Paraburkholderia phenoliruptrix**
	Full Scientific Name (LPSN)	Paraburkholderia phenoliruptrix (Coenye et al. 2005) Sawana et al. 2015

Synonym

Burkholderia phenoliruptrix

Information on morphological and physiological properties Morphology

[Ref.: #121796]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.965

[Ref.: #121796]	Cell shape	rod-shaped

[Ref.: #121796]	Motility	no
[Ref.: #69480]	Motility	yes Confidence in %95.781

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7177]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #7177]

Culture medium growth

[Ref.: #7177]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7177]

Culture medium composition

expand / minimize

[Ref.: #7177]

Culture medium

MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457)

[Ref.: #7177]

Culture medium growth

[Ref.: #7177]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7177]

Culture medium composition

expand / minimize

[Ref.: #41977]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #41977]

Culture medium growth

[Ref.: #41977]

Culture medium composition

expand / minimize

[Ref.: #121796]

Culture medium

CIP Medium 72

[Ref.: #121796]

Culture medium growth

[Ref.: #121796]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #7177]		growth	28 °C
[Ref.: #41977]		growth	30 °C
[Ref.: #58230]		growth	30 °C
[Ref.: #121796]		growth	25-37 °C
[Ref.: #121796]	no	growth	5 °C
[Ref.: #121796]	no	growth	10 °C
[Ref.: #121796]	no	growth	41 °C

[Ref.: #7177]	Temperature range	mesophilic
[Ref.: #41977]	Temperature range	mesophilic
[Ref.: #58230]	Temperature range	mesophilic
[Ref.: #121796]	Temperature range	mesophilic
[Ref.: #121796]	Temperature range	psychrophilic
[Ref.: #121796]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #58230]	Oxygen tolerance	aerobe
[Ref.: #121796]	Oxygen tolerance	obligate aerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.987

	Salt	Tested relation		Salt conc.
[Ref.: #121796]	NaCl		growth	0-2	%
[Ref.: #121796]	NaCl	no	growth	4	%
[Ref.: #121796]	NaCl	no	growth	6	%
[Ref.: #121796]	NaCl	no	growth	8	%
[Ref.: #121796]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #121796]	esculin ChEBI	-	hydrolysis
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #121796]	nitrate ChEBI	-	reduction
[Ref.: #121796]	nitrate ChEBI	-	respiration
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #121796]	nitrite ChEBI	-	reduction
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #121796]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #121796]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #121796]	amylase	+
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #121796]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym, API 20NE
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121796]	caseinase	-	3.4.21.50
[Ref.: #121796]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #121796]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #121796]	gelatinase	-
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #121796]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #121796]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #121796]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121796]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #121796]	oxidase	+
[Ref.: #121796]	protease	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #121796]	tryptophan deaminase	-
[Ref.: #121796]	tween esterase	-
[Ref.: #121796]	urease	-	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #7177]	23792	-	-	-	-	-	-	-	+	+	+	+	+	+	-	+	+/-	+/-	+	+	+	+/-

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #121796]	2818	not determinedn.d.	+	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	-	+	+	-	-	-	-	+	-	-	-	+	-	-	+	+	+	-	+	-	+	+	-	-	-	-	-	-	-	-	+	+	-	+	-	-	-	-	-	+	-	-	-	-	-	+	-	+	+	-	-	-	-	+	-	-	+	+	-	-	-	-	-	-	-	+	+	-	+	-	-	-	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121796]	11312	-	+	+	+	-	+	-	-	-	-	+	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7177]	Sample type/isolated from	chemostat with 2,4,5-trichlorophenoxyacetic acid
[Ref.: #7177]	Geographic location (country and/or sea, region)	Illionis
[Ref.: #7177]	Country	USA
[Ref.: #7177]	Country ISO 3 Code	USA
[Ref.: #7177]	Continent	North America

[Ref.: #58230]	Sample type/isolated from	Chemostat grown with 2,4,5-trichlorophenoxyacetic
[Ref.: #58230]	Geographic location (country and/or sea, region)	Vancouver
[Ref.: #58230]	Country	Canada
[Ref.: #58230]	Country ISO 3 Code	CAN
[Ref.: #58230]	Continent	North America

[Ref.: #121796]	Sample type/isolated from	Chemostat grown with 2 4 5-trichlorophenoxyacetic
[Ref.: #121796]	Geographic location (country and/or sea, region)	Vancouver
[Ref.: #121796]	Country	Canada
[Ref.: #121796]	Country ISO 3 Code	CAN
[Ref.: #121796]	Continent	North America
* marker position based on {}

Isolation sources categories

#Engineered

#Bioreactor

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7177]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121796]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7177]	Burkholderia sp. AC1100 16S ribosomal RNA gene, partial sequence	AY435213	1485	ENA	252970 tax ID
[Ref.: #20218]	Burkholderia phenoliruptrix strain LMG 22037 16S ribosomal RNA gene, partial sequence	HQ849094	1126	ENA	252970 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Paraburkholderia phenoliruptrix LMG 22037	GCA_902833655	contig	GenBank	252970 tax ID	*
[Ref.: #66792]	Paraburkholderia phenoliruptrix LMG 22037	GCA_902859825	contig	GenBank	252970 tax ID	*
[Ref.: #66792]	Paraburkholderia phenoliruptrix AC1100	GCA_000416445	contig	GenBank	1337996 tax ID	*
[Ref.: #66792]	Paraburkholderia phenoliruptrix strain LMG 22037	252970.3	wgs	PATRIC	252970 tax ID	*
[Ref.: #66792]	Paraburkholderia phenoliruptrix strain LMG 22037	252970.5	wgs	PATRIC	252970 tax ID	*
[Ref.: #66792]	Paraburkholderia phenoliruptrix AC1100	2554235175	draft	JGI IMG/M	1337996 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Paraburkholderia phenoliruptrix strain LMG 22037 PATRIC: 252970.4
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Paraburkholderia phenoliruptrix strain LMG 22037 PATRIC: 252970.3
[Ref.: #69480]	motile	yes	88.86	no
[Ref.: #69480]	gram-positive	no	98.098	no
[Ref.: #69480]	anaerobic	no	98.799	no
[Ref.: #69480]	aerobic	yes	86.958	yes
[Ref.: #69480]	halophile	no	95.061	no
[Ref.: #69480]	spore-forming	no	92.72	no
[Ref.: #69480]	glucose-util	yes	89.84	yes
[Ref.: #69480]	flagellated	no	85.166	no
[Ref.: #69480]	thermophile	no	99.425	yes
[Ref.: #69480]	glucose-ferment	no	89.958	yes

Availability in culture collections External links

[Ref.: #7177]

Culture collection no.

DSM 17773, CCUG 48558, CIP 108591, LMG 22037

[Ref.: #71612]

SI-ID 43466

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Catalytic mechanism of 5-chlorohydroxyhydroquinone dehydrochlorinase from the YCII superfamily of largely unknown function.	Hayes RP, Lewis KM, Xun L, Kang C	J Biol Chem	10.1074/jbc.M113.499368	2013	*
Genetics	Genome Sequence of the 2,4,5-Trichlorophenoxyacetate-Degrading Bacterium Burkholderia phenoliruptrix Strain AC1100.	Xu P, Yu H, Chakrabarty AM, Xun L	Genome Announc	10.1128/genomeA.00600-13	2013	*
Phylogeny	Burkholderia phenoliruptrix sp. nov., to accommodate the 2,4,5-trichlorophenoxyacetic acid and halophenol-degrading strain AC1100.	Coenye T, Henry D, Speert DP, Vandamme P	Syst Appl Microbiol	10.1078/0723202042369992	2004	*

References

#7177

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17773

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#41977 ; Curators of the CIP;
#58230 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 48558

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#71612

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID43466.1 )

#121796 Collection of Institut Pasteur ; Curators of the CIP; CIP 108591
* These data were automatically processed and therefore are not curated

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Paraburkholderia phenoliruptrix strain LMG 22037

Paraburkholderia phenoliruptrix strain LMG 22037

Change proposal

Change proposal