Paraburkholderia kururiensis (DSM 13646, ATCC 700977, CCUG 43663)

Name: Paraburkholderia kururiensis (DSM 13646, ATCC 700977, CCUG 43663)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1943

Type strain

Strain Designation KP23

Culture col. no. DSM 13646 ATCC 700977 CCUG 43663 CIP 106643 JCM 10599 4 more

NCBI tax ID(s) 1236956 984307 89498

Special Collections DSMZ CCUG JCM CIP

History DSMZ <- JCM <- Y. Kamagata; KP23 JCM <-- Y. Kamagata KP23. CIP <- 2001, JCM <- Y. Kamagata: strain KP23

Links

Paraburkholderia kururiensis KP23 is an aerobe, Gram-negative, rod-shaped bacterium that was isolated from aquifer sample from TCE polluted site.

Gram-negative rod-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 13210

LMG 19447,JCM 10599,ATCC 700977,DSM 13646,CIP 106643,CCM 7121,CCUG 43663,NCIMB 13885

@ref 20215

Last LPSN update 2026-05-29 08:25:22

Domain Bacteria

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Burkholderiaceae

Genus Paraburkholderia

Species Paraburkholderia kururiensis

Full scientific name Paraburkholderia kururiensis (Zhang et al. 2000) Sawana et al. 2015

Synonyms (1)

Burkholderia kururiensis

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
120516	negative	rod-shaped
125438	negative		96.482
125439	negative		96.718

Colony morphology

55989

Incubation period2-3 days

Pigmentation

@ref	Production	Name
120516		Pyocyanin

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
4939	CASO AGAR (MERCK 105458) (DSMZ Medium 220)	Medium recipe at MediaDive	Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
4939	MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457)	Medium recipe at MediaDive	Name: MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457; with strain-specific modifications) Composition: Na2HPO4 2.44 g/l KH2PO4 1.52 g/l (NH4)2SO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l Phenol 0.15 g/l CaCl2 x 2 H2O 0.05 g/l EDTA 0.005 g/l FeSO4 x 7 H2O 0.002 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l MnCl2 x 4 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Distilled water
4939	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
4939	TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545)	Medium recipe at MediaDive	Name: TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545) Composition: Casein peptone 17.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water
41962	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
120516	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
4939	positive	growth	30
4939	positive	growth	28
41962	positive	growth	37
55989	positive	growth	30
67770	positive	growth	37
120516	positive	growth	25-41
120516	negative	growth	5
120516	negative	growth	10

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
55989	aerobe
120516	facultative anaerobe
125439	obligate aerobe	96.526

Spore formation

@ref	Spore formation	Confidence
125439		98.846

Halophily

@ref	Salt	Growth	Tested relation	Concentration
120516	NaCl	positive	growth	0-4 %
120516	NaCl		growth	6 %
120516	NaCl		growth	8 %
120516	NaCl		growth	10 %

Observation

67770

Observationquinones: Q-8

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
120516	16947 ChEBI	citrate	+	carbon source
120516	4853 ChEBI	esculin	+	hydrolysis
120516	17632 ChEBI	nitrate	+	reduction
120516	17632 ChEBI	nitrate	+	respiration
120516	16301 ChEBI	nitrite	+	reduction

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
120516	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
120516	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
120516	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
120516	amylase	+
68382	beta-galactosidase	-	3.2.1.23	from API zym
120516	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
120516	caseinase	+	3.4.21.50
120516	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
120516	DNase	+
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
120516	gelatinase	+
120516	lecithinase	+
68382	leucine arylamidase	+	3.4.11.1	from API zym
120516	lipase	-
68382	lipase (C 14)	-		from API zym
120516	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
120516	ornithine decarboxylase	-	4.1.1.17
120516	oxidase	-
120516	protease	-
68382	trypsin	-	3.4.21.4	from API zym
120516	tryptophan deaminase	-
120516	tween esterase	-
120516	urease	+	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
120516	-	+	+	+	-	+	-	-	-	-	+	+	-	-	-	-	+	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
120516	not determinedn.d.	+	+	+	+	+	-	-	-	-	+	+	-	-	+	-	+	-	-	+	+	+	+	-	+	+	-	+	+	+	-	-	+	+	+	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	+	+	+	+	+	-	+	+	+	+	+	+	+	-	-	+	+	+	+	-	+	-	+	-	-	-	+	+	-	-	-	+	-	+	-	+	+	+	+	-	+	+	-	-	+	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Groundwater

Isolation

@ref	Sample type	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
4939	aquifer sample from TCE polluted site		Kururi	Japan	JPN	Asia
55989	Aquifer,trichloroethylene polluted site	1996	Kururi Chiba Prefecture	Japan	JPN	Asia
67770	Aquifer sample collected from a TCE polluted site		Kururi, Chiba Pref.	Japan	JPN	Asia
120516	Aquifer sample collected from a TCE-polluted site		Kururi, Chiba	Japan	JPN	Asia	1996

Taxonmaps

69479

Global distribution of 16S sequence AB024310 (>99% sequence identity) for Paraburkholderia kururiensis subclade from Microbeatlas

Aquatic Samples	495
Soil Samples	1489
Animal Samples	393
Plant Samples	818
Total Samples	3195

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
4939	1	Risk group (German classification) According to TRBA 466
120516	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	Paraburkholderia kururiensis LMG 19447 assembly for Paraburkholderia kururiensis JCM 10599 = LMG 19447	contig	GCA_902833705	1236956.13	8111909995	1236956	60.08
66792	ASM398693v1 assembly for Paraburkholderia kururiensis JCM 10599 = LMG 19447 KP23	contig	GCA_003986935	984307.21	8116899607	1236956	49.38
66792	ASM61602v1 assembly for Paraburkholderia kururiensis JCM 10599 = LMG 19447	contig	GCA_000616025	1236956.7	2579778690	1236956	0

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
4939	Burkholderia kururiensis gene for 16S ribosomal RNA	AB024310	1461		984307
124043	Burkholderia kururiensis strain LMG 19447 16S ribosomal RNA gene, partial sequence.	HQ849088	1124		1236956

GC content

@ref	GC-content (mol%)	Method
4939	64.8	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Paraburkholderia kururiensis LMG 19447 assembly for Paraburkholderia kururiensis JCM 10599 = LMG 19447
BV-BRC: 1236956.13

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	96.53	no
125439	gram_stain	BacteriaNetⓘ	negative	96.72	no
125439	motility	BacteriaNetⓘ	yes	63.45	no
125439	spore_formation	BacteriaNetⓘ	no	98.85	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Burkholderia kururiensis JCM 10599
PATRIC: 1236956.7

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	96.48	no
125438	anaerobic	anaerobicⓘ	no	96.15	yes
125438	spore-forming	spore-formingⓘ	no	86.74	no
125438	aerobic	aerobicⓘ	yes	89.72	no
125438	thermophilic	thermophileⓘ	no	96.50	yes
125438	flagellated	motile2+ⓘ	yes	81.97	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Burkholderia thailandensis E264 as a promising safe rhamnolipids' producer towards a sustainable valorization of grape marcs and olive mill pomace.	Chebbi A, Tazzari M, Rizzi C, Gomez Tovar FH, Villa S, Sbaffoni S, Vaccari M, Franzetti A.	Appl Microbiol Biotechnol	10.1007/s00253-021-11292-0	2021
	Molecular signatures and phylogenomic analysis of the genus Burkholderia: proposal for division of this genus into the emended genus Burkholderia containing pathogenic organisms and a new genus Paraburkholderia gen. nov. harboring environmental species.	Sawana A, Adeolu M, Gupta RS.	Front Genet	10.3389/fgene.2014.00429	2014
	Iterative glycosylation on a single residue of a mature lasso peptide.	Sun K, Cui JJ, Zhai W, Su X, Liu YC, Ning L, Xiong J, Gao K, Luo S, Lei X, Dong SH.	Chem Sci	10.1039/d5sc00605h	2025
Phylogeny	Reclassification of Phycicola gilvus (Lee et al. 2008) and Leifsonia pindariensis (Reddy et al. 2008) as Microterricola gilva comb. nov. and Microterricola pindariensis comb. nov. and emended description of the genus Microterricola.	Dhotre DP, Rajabal V, Sharma A, Kulkarni GJ, Prakash O, Vemuluri VR, Joseph N, Rahi P, Shouche YS.	Int J Syst Evol Microbiol	10.1099/ijsem.0.002019	2017
Genetics	Genome-Wide Identification and Characterization of Homeobox Transcription Factors in Phoma sorghina var. saccharum Causing Sugarcane Twisted Leaf Disease.	Bao Y, Deng J, Akbar S, Duan Z, Zhang C, Lin W, Wu S, Yue Y, Yao W, Xu J, Zhang M.	Int J Mol Sci	10.3390/ijms25105346	2024
Enzymology	Detection, distribution, and organohalogen compound discovery implications of the reduced flavin adenine dinucleotide-dependent halogenase gene in major filamentous actinomycete taxonomic groups.	Gao P, Huang Y.	Appl Environ Microbiol	10.1128/aem.02958-08	2009
	Genomic Assemblies of Members of Burkholderia and Related Genera as a Resource for Natural Product Discovery.	Mullins AJ, Jones C, Bull MJ, Webster G, Parkhill J, Connor TR, Murray JAH, Challis GL, Mahenthiralingam E.	Microbiol Resour Announc	10.1128/mra.00485-20	2020
	Diaminobutyricibacter tongyongensis gen. nov., sp. nov. and Homoserinibacter gongjuensis gen. nov., sp. nov. belong to the family Microbacteriaceae.	Kim SJ, Ahn JH, Weon HY, Hamada M, Suzuki K, Kwon SW.	J Microbiol	10.1007/s12275-014-3278-y	2014
Phylogeny	Brachybacterium saurashtrense sp. nov., a halotolerant root-associated bacterium with plant growth-promoting potential.	Gontia I, Kavita K, Schmid M, Hartmann A, Jha B.	Int J Syst Evol Microbiol	10.1099/ijs.0.023176-0	2011
Phylogeny	Mixotrophic metabolism in Burkholderia kururiensis subsp. thiooxydans subsp. nov., a facultative chemolithoautotrophic thiosulfate oxidizing bacterium isolated from rhizosphere soil and proposal for classification of the type strain of Burkholderia kururiensis as Burkholderia kururiensis subsp. kururiensis subsp. nov.	Anandham R, Indira Gandhi P, Kwon SW, Sa TM, Kim YK, Jee HJ	Arch Microbiol	10.1007/s00203-009-0517-4	2009
Phylogeny	Burkholderia kururiensis sp. nov., a trichloroethylene (TCE)-degrading bacterium isolated from an aquifer polluted with TCE.	Zhang H, Hanada S, Shigematsu T, Shibuya K, Kamagata Y, Kanagawa T, Kurane R	Int J Syst Evol Microbiol	10.1099/00207713-50-2-743	2000

References

#4939	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13646
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#41962	; Curators of the CIP;
#55989	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 43663
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120516	Collection of Institut Pasteur ; Curators of the CIP; CIP 106643
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Paraburkholderia kururiensis (DSM 13646, ATCC 700977, CCUG 43663)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Antibiotic resistance

Metabolite production

Enzymes

API zym

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_902833705

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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