Name: Paraburkholderia kururiensis KP23
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 1943

Type strain:

Species: Paraburkholderia kururiensis

Strain Designation: KP23

Culture col. no.: DSM 13646, ATCC 700977, CCUG 43663, CIP 106643, JCM 10599, LMG 19447, CCM 7121, CGMCC 1.10442

Strain history: CIP <- 2001, JCM <- Y. Kamagata: strain KP23

NCBI tax ID(s): 1236956 (strain), 984307 (species), 89498 (subspecies)

SI-ID 13210

LMG 19447, JCM 10599, ATCC 700977, DSM 13646, CIP 106643, CCM 7121, CCUG 43663, NCIMB 13885

Special Collections

Section

When using BacDive for research please cite our paper

Paraburkholderia kururiensis KP23 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from aquifer sample from TCE polluted site.

Gram-negative
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Burkholderiaceae*
	Genus	*Paraburkholderia*
	Species	**Paraburkholderia kururiensis**
	Full Scientific Name (LPSN)	Paraburkholderia kururiensis (Zhang et al. 2000) Sawana et al. 2015

Synonym

Burkholderia kururiensis

Information on morphological and physiological properties Morphology

[Ref.: #120516]	Gram stain	negative
[Ref.: #125438]	Gram stain	negative Confidence in %96.482
[Ref.: #125439]	Gram stain	negative Confidence in %98.9

[Ref.: #120516]	Cell shape	rod-shaped

[Ref.: #120516]	Motility	no

[Ref.: #55989]

Incubation period

2-3 days

[Ref.: #120516]	Pigment production	no
[Ref.: #120516]	Pigment name	Pyocyanin

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4939]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #4939]

Culture medium growth

[Ref.: #4939]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4939]

Culture medium composition

expand / minimize

[Ref.: #4939]

Culture medium

MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457)

[Ref.: #4939]

Culture medium growth

[Ref.: #4939]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4939]

Culture medium composition

expand / minimize

[Ref.: #4939]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #4939]

Culture medium growth

[Ref.: #4939]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #4939]

Culture medium

TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545)

[Ref.: #4939]

Culture medium growth

[Ref.: #4939]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #41962]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #41962]

Culture medium growth

[Ref.: #41962]

Culture medium composition

expand / minimize

[Ref.: #120516]

Culture medium

CIP Medium 72

[Ref.: #120516]

Culture medium growth

[Ref.: #120516]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #4939]		growth	30 °C
[Ref.: #4939]		growth	28 °C
[Ref.: #41962]		growth	37 °C
[Ref.: #55989]		growth	30 °C
[Ref.: #67770]		growth	37 °C
[Ref.: #120516]		growth	25-41 °C
[Ref.: #120516]	negative	growth	5 °C
[Ref.: #120516]	negative	growth	10 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #55989]	Oxygen tolerance	aerobe
[Ref.: #120516]	Oxygen tolerance	facultative anaerobe
[Ref.: #125439]	Oxygen tolerance	obligate aerobe Confidence in %96.5

[Ref.: #125439]

Ability of spore formation

no Confidence in %96.4

	Salt	Tested relation		Salt conc.
[Ref.: #120516]	NaCl		growth	0-4	%
[Ref.: #120516]	NaCl	no	growth	6	%
[Ref.: #120516]	NaCl	no	growth	8	%
[Ref.: #120516]	NaCl	no	growth	10	%

[Ref.: #67770]

Observation

quinones: Q-8

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #120516]	citrate ChEBI	+	carbon source
[Ref.: #120516]	esculin ChEBI	+	hydrolysis
[Ref.: #120516]	nitrate ChEBI	+	reduction
[Ref.: #120516]	nitrate ChEBI	+	respiration
[Ref.: #120516]	nitrite ChEBI	+	reduction

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #120516]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #120516]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #120516]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #120516]	amylase	+
[Ref.: #120516]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #120516]	caseinase	+	3.4.21.50
[Ref.: #120516]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #120516]	DNase	+
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #120516]	gelatinase	+
[Ref.: #120516]	lecithinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #120516]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #120516]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #120516]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #120516]	oxidase	-
[Ref.: #120516]	protease	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #120516]	tryptophan deaminase	-
[Ref.: #120516]	tween esterase	-
[Ref.: #120516]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #120516]	1752	not determinedn.d.	+	+	+	+	+	-	-	-	-	+	+	-	-	+	-	+	-	-	+	+	+	+	-	+	+	-	+	+	+	-	-	+	+	+	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	+	+	+	+	+	-	+	+	+	+	+	+	+	-	-	+	+	+	+	-	+	-	+	-	-	-	+	+	-	-	-	+	-	+	-	+	+	+	+	-	+	+	-	-	+	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120516]	9692	-	+	+	+	-	+	-	-	-	-	+	+	-	-	-	-	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4939]	Sample type/isolated from	aquifer sample from TCE polluted site
[Ref.: #4939]	Geographic location (country and/or sea, region)	Kururi
[Ref.: #4939]	Country	Japan
[Ref.: #4939]	Country ISO 3 Code	JPN
[Ref.: #4939]	Continent	Asia

[Ref.: #55989]	Sample type/isolated from	Aquifer,trichloroethylene polluted site
[Ref.: #55989]	Sampling date	1996
[Ref.: #55989]	Geographic location (country and/or sea, region)	Kururi Chiba Prefecture
[Ref.: #55989]	Country	Japan
[Ref.: #55989]	Country ISO 3 Code	JPN
[Ref.: #55989]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Aquifer sample collected from a TCE polluted site
[Ref.: #67770]	Geographic location (country and/or sea, region)	Kururi, Chiba Pref.
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia

[Ref.: #120516]	Sample type/isolated from	Aquifer sample collected from a TCE-polluted site
[Ref.: #120516]	Geographic location (country and/or sea, region)	Kururi, Chiba
[Ref.: #120516]	Country	Japan
[Ref.: #120516]	Country ISO 3 Code	JPN
[Ref.: #120516]	Continent	Asia
[Ref.: #120516]	Isolation date	1996
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Groundwater

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB024310 (>99% sequence identity) for Paraburkholderia kururiensis subclade from Microbeatlas

Aquatic Samples	495
Soil Samples	1489
Animal Samples	393
Plant Samples	818
Total Samples	3195

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4939]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120516]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #4939]	Burkholderia kururiensis gene for 16S ribosomal RNA	AB024310	1461	GenBank ENA	984307 tax ID
[Ref.: #124043]	Burkholderia kururiensis strain LMG 19447 16S ribosomal RNA gene, partial sequence.	HQ849088	1124	GenBank ENA	1236956 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Paraburkholderia kururiensis JCM 10599 = LMG 19447	GCA_902833705	contig	GenBank ENA	1236956 tax ID	*
[Ref.: #66792]	Paraburkholderia kururiensis JCM 10599 = LMG 19447 KP23	GCA_003986935	contig	GenBank ENA	1236956 tax ID	*
[Ref.: #66792]	Paraburkholderia kururiensis JCM 10599 = LMG 19447	GCA_000616025	contig	GenBank ENA	1236956 tax ID	*
[Ref.: #66792]	Burkholderia kururiensis JCM 10599	1236956.7	wgs	PATRIC	1236956 tax ID	*
[Ref.: #66792]	Paraburkholderia kururiensis JCM 10599 = LMG 19447	1236956.13	wgs	PATRIC	1236956 tax ID	*
[Ref.: #66792]	Paraburkholderia kururiensis strain KP23	984307.21	wgs	PATRIC	984307 tax ID	*

[Ref.: #4939]

GC-content

64.8 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %	In training data
	Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Burkholderia kururiensis JCM 10599 PATRIC: 1236956.7
[Ref.: #125438]	gram-positive	gram-positiveⓘ	no	96.482	no
[Ref.: #125438]	anaerobic	anaerobicⓘ	no	96.151	yes
[Ref.: #125438]	spore-forming	spore-formingⓘ	no	86.74	no
[Ref.: #125438]	aerobic	aerobicⓘ	yes	89.716	no
[Ref.: #125438]	thermophile	thermophilicⓘ	no	96.5	yes
[Ref.: #125438]	motile2+	flagellatedⓘ	yes	81.966	no

	Model	Trait	Prediction	Confidence in %
	Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Paraburkholderia kururiensis JCM 10599 = LMG 19447 NCBI: GCA_902833705
[Ref.: #125439]	BacteriaNet	oxygen_toleranceⓘ	obligate aerobe	96.5
[Ref.: #125439]	BacteriaNet	gram_stainⓘ	negative	98.9
[Ref.: #125439]	BacteriaNet	motilityⓘ	yes	71.6
[Ref.: #125439]	BacteriaNet	spore_formationⓘ	no	96.4

Availability in culture collections External links

[Ref.: #4939]

Culture collection no.

DSM 13646, ATCC 700977, CCUG 43663, CIP 106643, JCM 10599, LMG 19447, CCM 7121, CGMCC 1.10442

[Ref.: #71588]

SI-ID 13210

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Mixotrophic metabolism in Burkholderia kururiensis subsp. thiooxydans subsp. nov., a facultative chemolithoautotrophic thiosulfate oxidizing bacterium isolated from rhizosphere soil and proposal for classification of the type strain of Burkholderia kururiensis as Burkholderia kururiensis subsp. kururiensis subsp. nov.	Anandham R, Indira Gandhi P, Kwon SW, Sa TM, Kim YK, Jee HJ	Arch Microbiol	10.1007/s00203-009-0517-4	2009	*
Phylogeny	Burkholderia kururiensis sp. nov., a trichloroethylene (TCE)-degrading bacterium isolated from an aquifer polluted with TCE.	Zhang H, Hanada S, Shigematsu T, Shibuya K, Kamagata Y, Kanagawa T, Kurane R	Int J Syst Evol Microbiol	10.1099/00207713-50-2-743	2000	*

References

#4939

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13646

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#41962 ; Curators of the CIP;
#55989 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 43663

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#71588

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID13210.1 )

#120516 Collection of Institut Pasteur ; Curators of the CIP; CIP 106643

#124043

Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

#125438

Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )

#125439

Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

* These data were automatically processed and therefore are not curated

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Burkholderia kururiensis JCM 10599

Paraburkholderia kururiensis JCM 10599 = LMG 19447

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Change proposal