Name: Burkholderia thailandensis E264
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1942

Type strain:

Species: Burkholderia thailandensis

Strain Designation: E264

Culture col. no.: CCUG 48851, CIP 106301, DSM 13276, ATCC 700388

Strain history: CIP <- 1999, D.E. Woods, Univ. of Calgary Health Sci. Centre, Canada <- 1995, N.J. White: strain E264

NCBI tax ID(s): 271848 (strain), 57975 (species)

SI-ID 46232

ATCC 700388, LMG 20219, CIP 106301, DSM 13276, CBMAI 690, CCUG 48851, VTT E-082813

Other strains from Burkholderia thailandensis

B. thailandensis STI41339(IMET),
B. thailandensis E265, CIP 106302

Section

Burkholderia thailandensis E264 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from environmental sample.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Burkholderiaceae*
	Genus	*Burkholderia*
	Species	**Burkholderia thailandensis**
	Full Scientific Name (LPSN)	Burkholderia thailandensis Brett et al. 1998

Information on morphological and physiological properties Morphology

[Ref.: #123492]	Gram stain	negative

[Ref.: #123492]	Cell shape	rod-shaped

[Ref.: #123492]	Motility	yes

[Ref.: #4988]	Type of hemolysis	gamma
[Ref.: #4988]	Incubation period	1-2 days

[Ref.: #123492]	Pigment production	no
[Ref.: #123492]	Pigment name	Pyocyanin

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4988]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #4988]

Culture medium growth

[Ref.: #4988]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4988]

Culture medium composition

expand / minimize

[Ref.: #4988]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #4988]

Culture medium growth

[Ref.: #4988]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4988]

Culture medium composition

expand / minimize

[Ref.: #4988]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #4988]

Culture medium growth

[Ref.: #4988]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4988]

Culture medium composition

expand / minimize

[Ref.: #39485]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #39485]

Culture medium growth

[Ref.: #39485]

Culture medium composition

expand / minimize

[Ref.: #123492]

Culture medium

CIP Medium 72

[Ref.: #123492]

Culture medium growth

[Ref.: #123492]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #4988]		growth	28 °C
[Ref.: #39485]		growth	30 °C
[Ref.: #58359]		growth	30-37 °C
[Ref.: #123492]		growth	25-41 °C
[Ref.: #123492]	no	growth	5 °C
[Ref.: #123492]	no	growth	10 °C

[Ref.: #4988]	Temperature range	mesophilic
[Ref.: #39485]	Temperature range	mesophilic
[Ref.: #58359]	Temperature range	mesophilic
[Ref.: #123492]	Temperature range	psychrophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #58359]	Oxygen tolerance	aerobe
[Ref.: #123492]	Oxygen tolerance	obligate aerobe

	Salt	Tested relation		Salt conc.
[Ref.: #123492]	NaCl		growth	0-4	%
[Ref.: #123492]	NaCl	no	growth	6	%
[Ref.: #123492]	NaCl	no	growth	8	%
[Ref.: #123492]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	+	assimilation	* from API 20NE
[Ref.: #123492]	citrate ChEBI	+	carbon source
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #123492]	esculin ChEBI	-	hydrolysis
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #123492]	nitrate ChEBI	+	reduction
[Ref.: #123492]	nitrate ChEBI	+	respiration
[Ref.: #123492]	nitrite ChEBI	+	reduction
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #123492]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #123492]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #123492]	alcohol dehydrogenase	+	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #123492]	amylase	-
[Ref.: #123492]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #123492]	caseinase	+	3.4.21.50
[Ref.: #123492]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #4988]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #123492]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #123492]	gelatinase	+
[Ref.: #68369]	gelatinase	+		* from API 20NE
[Ref.: #123492]	lecithinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #123492]	lipase	+
[Ref.: #68382]	lipase (C 14)	+		* from API zym
[Ref.: #123492]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #123492]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #123492]	oxidase	+
[Ref.: #123492]	protease	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #123492]	tryptophan deaminase	-
[Ref.: #123492]	tween esterase	+
[Ref.: #123492]	urease	-	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #4988]	24699	+	-	-	+	-	+/-	+	-	+	+	+	+	+	-	+	+	+	+	+	+	+
[Ref.: #4988]	23704	+	-	-	+	-	-	+	-	+	+	+	+	+	-	+	+	+	+	+	+	+
[Ref.: #4988]	23703	-	-	-	-	-	-	+	-	+	+	+	+	+	-	+	+	+	+	+/-	+	+
[Ref.: #4988]	23702	+	-	-	+	-	+/-	+	-	+	+	+	+	+	-	+	+	+	+	+	+	not determinedn.d.
[Ref.: #4988]	23701	+	-	-	+	-	+	+	-	+	+	+	+	+	-	+	+	+	+	+	+	+

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #123492]	1630	not determinedn.d.	+	+	+	+	+	-	-	+	-	+	-	-	-	-	-	+	+	-	+	+	+	+	-	-	+	-	+	+	+	+	+	+	+	+	-	-	+	+	-	-	-	-	-	+	-	-	-	-	-	+	+	+	-	+	-	-	+	+	-	+	+	+	+	+	+	+	-	+	-	-	-	+	+	+	-	+	+	+	-	+	+	+	+	+	+	+	+	-	+	+	+	+	+	+	+	+	+	+	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123492]	9382	-	+	+	+	+	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4988]	Sample type/isolated from	environmental sample
[Ref.: #4988]	Country	Thailand
[Ref.: #4988]	Country ISO 3 Code	THA
[Ref.: #4988]	Continent	Asia

[Ref.: #58359]	Sample type/isolated from	Soil,rice field
[Ref.: #58359]	Sampling date	1995
[Ref.: #58359]	Country	Thailand
[Ref.: #58359]	Country ISO 3 Code	THA
[Ref.: #58359]	Continent	Asia

[Ref.: #123492]	Sample type/isolated from	Environment, Rice field soil
[Ref.: #123492]	Country	Thailand
[Ref.: #123492]	Country ISO 3 Code	THA
[Ref.: #123492]	Continent	Asia
[Ref.: #123492]	Isolation date	1995
* marker position based on {}

Isolation sources categories

#Environmental

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4988]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #123492]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #4988]	Burkholderia thailandensis strain E264 16S ribosomal RNA gene, complete sequence	U91838	1488	ENA	271848 tax ID
[Ref.: #20218]	Burkholderia thailandensis strain E264 16S ribosomal RNA gene, partial sequence	EF535235	1541	ENA	271848 tax ID	*
[Ref.: #20218]	Burkholderia thailandensis strain BD10-00323 16S ribosomal RNA gene, partial sequence	KF444906	1459	ENA	57975 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Burkholderia thailandensis E264, ATCC 700388	637000052	complete	JGI IMG/M	271848 tax ID	*
[Ref.: #66792]	Burkholderia thailandensis E264 E264; ATCC 700388	GCA_000012365	complete	GenBank	271848 tax ID	*
[Ref.: #66792]	Burkholderia thailandensis E264 ATCC 700388	GCA_000152285	chromosome	GenBank	271848 tax ID	*
[Ref.: #66792]	Burkholderia thailandensis ATCC 700388	271848.8	wgs	PATRIC	271848 tax ID	*
[Ref.: #66792]	Burkholderia thailandensis Bt4	441164.8	wgs	PATRIC	441164 tax ID	*
[Ref.: #66792]	Burkholderia thailandensis E264	271848.6	complete	PATRIC	271848 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Burkholderia thailandensis E264 E264 NCBI: GCA_003568605.1
[Ref.: #69480]	motile	yes	84.722	no
[Ref.: #69480]	flagellated	no	78.626	no
[Ref.: #69480]	gram-positive	no	98.192	no
[Ref.: #69480]	anaerobic	no	97.357	yes
[Ref.: #69480]	aerobic	yes	85.543	yes
[Ref.: #69480]	halophile	no	94.25	no
[Ref.: #69480]	spore-forming	no	93.323	no
[Ref.: #69480]	glucose-util	yes	91.509	yes
[Ref.: #69480]	thermophile	no	99.481	yes
[Ref.: #69480]	glucose-ferment	no	87.958	no

Availability in culture collections External links

[Ref.: #4988]

Culture collection no.

CCUG 48851, CIP 106301, DSM 13276, ATCC 700388

[Ref.: #71587]

SI-ID 46232

Literature:

	Title	Authors	Journal	DOI	Year
Pathogenicity	Rhamnolipids from non-pathogenic Burkholderia thailandensis E264: Physicochemical characterization, antimicrobial and antibiofilm efficacy against oral hygiene related pathogens.	Elshikh M, Funston S, Chebbi A, Ahmed S, Marchant R, Banat IM	N Biotechnol	10.1016/j.nbt.2016.12.009	2017	*
Pathogenicity	Colonial morphotypes and biofilm forming ability of Burkholderia pseudomallei.	Koh SF, Tay ST, Puthucheary SD	Trop Biomed		2013	*
	Characterization of the Thermostable Biosurfactant Produced by Burkholderia thailandensis DSM 13276.	Gil CV, Rebocho AT, Esmail A, Sevrin C, Grandfils C, Torres CAV, Reis MAM, Freitas F	Polymers (Basel)	10.3390/polym14102088	2022	*
	Exometabolite Dynamics over Stationary Phase Reveal Strain-Specific Responses.	Chodkowski JL, Shade A	mSystems	10.1128/mSystems.00493-20	2020	*

References

#4988

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13276

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#39485 ; Curators of the CIP;
#58359 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 48851

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#71587

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID46232.1 )

#123492 Collection of Institut Pasteur ; Curators of the CIP; CIP 106301
* These data were automatically processed and therefore are not curated

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Burkholderia thailandensis E264 E264

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