Actinomyces massiliensis (DSM 23047, CCUG 53522, KCTC 15158)

Name: Actinomyces massiliensis (DSM 23047, CCUG 53522, KCTC 15158)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 192

Type strain

Strain Designation 4401292

Culture col. no. DSM 23047 CCUG 53522 KCTC 15158 CSUR P18 4401292 2 more

NCBI tax ID(s) 1167628 461393

Special Collections DSMZ CCUG JCM KCTC Literature strains CIP

History 2006, V. Roux, Timone Hosp., Marseille, France: strain 4401292 <- CCUG <- V. Roux, Timone Hospital, Marseille, France CCUG 53522 <-- V. Roux 4401292. <- DSM <- CCUG <- V Roux, Timone Hospital, Marseille, France CIP <- 2006, V. Roux, Timone Hosp., Marseille, France: strain 4401292

Links

Actinomyces massiliensis 4401292 is an anaerobe, mesophilic, Gram-positive prokaryote that was isolated from human blood .

Gram-positive rod-shaped anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 395408

KCTC 15158,JCM 16130,DSM 23047

@ref 20215

Last LPSN update 2026-02-09 11:17:49

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Actinomycetales

Family Actinomycetaceae

Genus Actinomyces

Species Actinomyces massiliensis

Full scientific name Actinomyces massiliensis Renvoise et al. 2009

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
28916	positive	0.5-1.7 µm	0.35-0.74 µm	rod-shaped
67771				rod-shaped
67771	positive
120847	positive			rod-shaped
125438					93.375
125438	positive				92.618
125439	positive				98.5

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
16669	PYG MEDIUM (MODIFIED) (DSMZ Medium 104)	Medium recipe at MediaDive	Name: PYG MEDIUM (modified) (DSMZ Medium 104; with strain-specific modifications) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 4.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
37528	MEDIUM 6 - Columbia agar with 10 % horse blood		Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
120847	CIP Medium 6	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
16669	positive	growth	37	mesophilic
28916	positive	growth	30-37	mesophilic
28916	positive	optimum	37	mesophilic
37528	positive	growth	30	mesophilic
59924	positive	growth	37	mesophilic
67770	positive	growth	37	mesophilic
67771	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
16669	anaerobe
28916	anaerobe
59924	microaerophile
67771	anaerobe
120847	facultative anaerobe

Spore formation

@ref	Spore formation
28916
67771

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68380	29016 ChEBI	arginine	-	hydrolysis	from API rID32A
28916	28757 ChEBI	fructose	+	carbon source
28916	28260 ChEBI	galactose	+	carbon source
28916	17234 ChEBI	glucose	+	carbon source
68380	29985 ChEBI	L-glutamate	-	degradation	from API rID32A
28916	17716 ChEBI	lactose	+	carbon source
28916	17306 ChEBI	maltose	+	carbon source
28916	37684 ChEBI	mannose	+	carbon source
28916	17632 ChEBI	nitrate	+	reduction
68380	17632 ChEBI	nitrate	+	reduction	from API rID32A
120847	17632 ChEBI	nitrate	+	reduction
120847	16301 ChEBI	nitrite	+	reduction
28916	33942 ChEBI	ribose	+	carbon source
28916	17992 ChEBI	sucrose	+	carbon source
28916	27082 ChEBI	trehalose	+	carbon source
68380	27897 ChEBI	tryptophan	-	energy source	from API rID32A
68380	16199 ChEBI	urea	-	hydrolysis	from API rID32A
28916	18222 ChEBI	xylose	+	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68380	35581 ChEBI	indole		from API rID32A
120847	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68380	35581 ChEBI	indole	-	from API rID32A

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68380	alanine arylamidase	-	3.4.11.2	from API rID32A
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68380	alkaline phosphatase	-	3.1.3.1	from API rID32A
68380	alpha-arabinosidase	-	3.2.1.55	from API rID32A
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68380	alpha-fucosidase	-	3.2.1.51	from API rID32A
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68380	alpha-galactosidase	-	3.2.1.22	from API rID32A
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68380	alpha-glucosidase	+	3.2.1.20	from API rID32A
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68380	arginine dihydrolase	-	3.5.3.6	from API rID32A
68382	beta-galactosidase	+	3.2.1.23	from API zym
68380	beta-galactosidase	+	3.2.1.23	from API rID32A
68380	beta-Galactosidase 6-phosphate	-		from API rID32A
68382	beta-glucosidase	+	3.2.1.21	from API zym
68380	beta-glucosidase	-	3.2.1.21	from API rID32A
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68380	beta-glucuronidase	-	3.2.1.31	from API rID32A
120847	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	-		from API zym
68380	glutamate decarboxylase	-	4.1.1.15	from API rID32A
68380	glutamyl-glutamate arylamidase	-		from API rID32A
68380	glycin arylamidase	-		from API rID32A
68380	histidine arylamidase	-		from API rID32A
68380	L-arginine arylamidase	-		from API rID32A
68382	leucine arylamidase	+	3.4.11.1	from API zym
68380	leucine arylamidase	+	3.4.11.1	from API rID32A
68380	leucyl glycin arylamidase	-	3.4.11.1	from API rID32A
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68380	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API rID32A
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
120847	oxidase	-
68380	phenylalanine arylamidase	+		from API rID32A
68380	proline-arylamidase	+	3.4.11.5	from API rID32A
68380	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32A
68380	serine arylamidase	-		from API rID32A
68382	trypsin	-	3.4.21.4	from API zym
68380	tryptophan deaminase	-	4.1.99.1	from API rID32A
68380	tyrosine arylamidase	+		from API rID32A
120847	urease	-	3.5.1.5
68380	urease	-	3.5.1.5	from API rID32A
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
120847	-	-	+	-	-	+	-	-	-	-	+	+	-	+	-	+	+	-	-	-

API rID32A

@ref	URE	ADH (Arg)	alpha GAL	beta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha GLU	beta GLU	alpha ARA	beta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	MNE	RAF	GDC	alpha FUC	Reduction of nitrateNIT	IND	PAL	L-arginine arylamidaseArgA	ProA	LGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	PyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
16669	-	-	-	+	-	+	-	-	-	-	+/-	+/-	-	-	+	-	-	-	+	-	+	+	-	+	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Infection	#Inflammation	-
#Host	#Human	-
#Host Body-Site	#Oral cavity and airways	#Lung
#Host Body Product	#Fluids	#Blood

Isolation

@ref	Sample type	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
16669	human blood (pleuropneumonia)		Marseille	France	FRA	Europe
59924	Human blood,39-yr-old man,pleuropneumonia	2004-05-01	Marseille,Timone hospital	France	FRA	Europe
67770	Human blood sample
67771	From human blood (pleuropneumonia)		Marseille	France	FRA	Europe
120847	Human, Blood		Marseille	France	FRA	Europe	2004

Taxonmaps

69479

Global distribution of 16S sequence AB545934 (>99% sequence identity) for Actinomyces massiliensis subclade from Microbeatlas

Aquatic Samples	1557
Soil Samples	995
Animal Samples	45755
Plant Samples	370
Total Samples	48677

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
16669	2	Risk group (German classification) According to TRBA 466
120847	2	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67771	ASM26980v1 assembly for Actinomyces massiliensis 4401292	scaffold	GCA_000269805	1167628.4	2548876973	1167628	72.52

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
16669	Actinomyces massiliensis strain 4401292 16S ribosomal RNA gene, partial sequence	EF558367	1481		1167628
67771	Actinomyces massiliensis gene for 16S ribosomal RNA, partial sequence	AB545934	1520		1167628

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Actinomyces massiliensis 4401292
NCBI: GCA_000269805

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	56.20	no
125439	motility	BacteriaNetⓘ	no	78.50	no
125439	gram_stain	BacteriaNetⓘ	positive	98.50	no
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	89.10	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Actinomyces massiliensis 4401292
PATRIC: 1167628.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	92.62	yes
125438	anaerobic	anaerobicⓘ	no	73.63	no
125438	spore-forming	spore-formingⓘ	no	78.85	yes
125438	aerobic	aerobicⓘ	no	76.63	yes
125438	thermophilic	thermophileⓘ	no	93.70	yes
125438	flagellated	motile2+ⓘ	no	93.38	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	The Impact of Maltitol-Sweetened Chewing Gum on the Dental Plaque Biofilm Microbiota Composition.	Keijser BJF, van den Broek TJ, Slot DE, van Twillert L, Kool J, Thabuis C, Ossendrijver M, van der Weijden FA, Montijn RC.	Front Microbiol	10.3389/fmicb.2018.00381	2018
Genetics	Draft genome sequence of Actinomyces massiliensis strain 4401292T.	Roux V, Robert C, Gimenez G, Gharbi R, Raoult D	J Bacteriol	10.1128/JB.01039-12	2012
Phylogeny	Actinomyces massiliensis sp. nov., isolated from a patient blood culture.	Renvoise A, Raoult D, Roux V	Int J Syst Evol Microbiol	10.1099/ijs.0.001503-0	2009

References

#16669	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23047
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#25355	IJSEM 540 2009 ( DOI 10.1099/ijs.0.001503-0 , PubMed 19244437 )
#28916	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25355
#37528	; Curators of the CIP;
#59924	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 53522
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#68380	Automatically annotated from API rID32A .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120847	Collection of Institut Pasteur ; Curators of the CIP; CIP 109360
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Actinomyces massiliensis (DSM 23047, CCUG 53522, KCTC 15158)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API rID32A

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000269805

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

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