Acetobacter oeni (DSM 23926, CECT 5830, LMG 21952)

Name: Acetobacter oeni (DSM 23926, CECT 5830, LMG 21952)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 19

Type strain

Strain Designation B13

Variant Isotype of BacDive ID 132838

Culture col. no. DSM 23926 CECT 5830 LMG 21952 B13 CIP 109171

NCBI tax ID(s) 1307912 304077

Special Collections DSMZ Literature strains CIP

Links

Acetobacter oeni B13 is an obligate aerobe, mesophilic, Gram-negative prokaryote that forms circular colonies and was isolated from spoiled red wine.

Gram-negative motile rod-shaped colony-forming obligate aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 100264

LMG 21952,CECT 5830,CIP 109171,DSM 23926,DSM 17891

@ref 20215

Last LPSN update 2025-12-16 09:46:45

Domain Pseudomonadati

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Rhodospirillales

Family Acetobacteraceae

Genus Acetobacter

Species Acetobacter oeni

Full scientific name Acetobacter oeni Silva et al. 2006

BacDive ID	Other strains from **Acetobacter oeni** (1)	Type strain
132838	A. oeni B 13, DSM 17891, CECT 5830, LMG 21952 (type strain)

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Flagellum arrangement	Confidence
23234	negative	3.6-5.1 µm	0.8-0.9 µm	rod-shaped	peritrichous
121399	negative			rod-shaped
125439	negative					99.7
125438	negative					97.833

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period	Medium used
23234	1-2 mm	cream, opaque	circular	4 days	YEDC

Multimedia

@ref:	17309
multimedia content:	DSM_23926.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_23926.jpg
caption:	Micrograph of DSM 23626. Scale bar represents 2 um.
intellectual property rights:	Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
manual_annotation:	1

@ref:	17309
multimedia content:	DSM_23926-1.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_23926-1.jpg
caption:	DSM 23926 grown on DSMZ meidum # 360 at 25°C.
intellectual property rights:	Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
17309	YPM MEDIUM (DSMZ Medium 360)	Medium recipe at MediaDive	Name: YPM MEDIUM (DSMZ Medium 360) Composition: Mannitol 25.0 g/l Agar 12.0 g/l Yeast extract 5.0 g/l Peptone 3.0 g/l Distilled water
23234	YEDC		7 g glucose, 3 g yeast extract, 20 g calcium carbonate and 17 g agar
37461	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
121399	CIP Medium 314	Medium recipe at CIP
121399	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
17309	positive	growth	25	mesophilic
23234	positive	optimum	28	mesophilic
37461	positive	growth	25	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
23234	obligate aerobe
121399	obligate aerobe
125439	obligate aerobe	98.6

Spore formation

@ref	Spore formation	Confidence
23234
125439		96.5

Tolerance

@ref	Compound	Percentage
23234	Ethanol	10

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23234	17754 ChEBI	glycerol	+	carbon source
23234	17306 ChEBI	maltose	-	carbon source
23234	17790 ChEBI	methanol	-	carbon source
121399	17632 ChEBI	nitrate	-	reduction
121399	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite
23234	16808 ChEBI	2-dehydro-D-gluconate
23234	17426 ChEBI	5-dehydro-D-gluconate
121399	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
121399	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
121399	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
121399	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	ethanol fermentation	100	2 of 2
66794	teichoic acid biosynthesis	100	1 of 1
66794	coenzyme A metabolism	100	4 of 4
66794	formaldehyde oxidation	100	3 of 3
66794	starch degradation	100	10 of 10
66794	butanoate fermentation	100	4 of 4
66794	biotin biosynthesis	100	4 of 4
66794	acetate fermentation	100	4 of 4
66794	adipate degradation	100	2 of 2
66794	threonine metabolism	100	10 of 10
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	ppGpp biosynthesis	100	4 of 4
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	methylglyoxal degradation	100	5 of 5
66794	ketogluconate metabolism	100	8 of 8
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	ceramide biosynthesis	100	1 of 1
66794	suberin monomers biosynthesis	100	2 of 2
66794	valine metabolism	100	9 of 9
66794	folate polyglutamylation	100	1 of 1
66794	quinate degradation	100	2 of 2
66794	palmitate biosynthesis	95.45	21 of 22
66794	Entner Doudoroff pathway	90	9 of 10
66794	glutamate and glutamine metabolism	89.29	25 of 28
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	lipid A biosynthesis	88.89	8 of 9
66794	allantoin degradation	88.89	8 of 9
66794	molybdenum cofactor biosynthesis	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	methionine metabolism	88.46	23 of 26
66794	vitamin B12 metabolism	88.24	30 of 34
66794	C4 and CAM-carbon fixation	87.5	7 of 8
66794	tetrahydrofolate metabolism	85.71	12 of 14
66794	propanol degradation	85.71	6 of 7
66794	photosynthesis	85.71	12 of 14
66794	phenylalanine metabolism	84.62	11 of 13
66794	vitamin B1 metabolism	84.62	11 of 13
66794	NAD metabolism	83.33	15 of 18
66794	pentose phosphate pathway	81.82	9 of 11
66794	glycogen metabolism	80	4 of 5
66794	peptidoglycan biosynthesis	80	12 of 15
66794	alanine metabolism	79.31	23 of 29
66794	purine metabolism	78.72	74 of 94
66794	citric acid cycle	78.57	11 of 14
66794	serine metabolism	77.78	7 of 9
66794	d-mannose degradation	77.78	7 of 9
66794	CMP-KDO biosynthesis	75	3 of 4
66794	isoleucine metabolism	75	6 of 8
66794	gluconeogenesis	75	6 of 8
66794	sulfopterin metabolism	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	histidine metabolism	72.41	21 of 29
66794	ubiquinone biosynthesis	71.43	5 of 7
66794	reductive acetyl coenzyme A pathway	71.43	5 of 7
66794	heme metabolism	71.43	10 of 14
66794	pyrimidine metabolism	71.11	32 of 45
66794	arginine metabolism	70.83	17 of 24
66794	glycolysis	70.59	12 of 17
66794	leucine metabolism	69.23	9 of 13
66794	cyanate degradation	66.67	2 of 3
66794	flavin biosynthesis	66.67	10 of 15
66794	octane oxidation	66.67	2 of 3
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	CO2 fixation in Crenarchaeota	66.67	6 of 9
66794	acetoin degradation	66.67	2 of 3
66794	non-pathway related	65.79	25 of 38
66794	tryptophan metabolism	65.79	25 of 38
66794	vitamin B6 metabolism	63.64	7 of 11
66794	metabolism of disaccharids	63.64	7 of 11
66794	proline metabolism	63.64	7 of 11
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
66794	degradation of sugar alcohols	62.5	10 of 16
66794	lipid metabolism	61.29	19 of 31
66794	cysteine metabolism	61.11	11 of 18
66794	oxidative phosphorylation	60.44	55 of 91
66794	cellulose degradation	60	3 of 5
66794	propionate fermentation	60	6 of 10
66794	degradation of aromatic, nitrogen containing compounds	58.33	7 of 12
66794	isoprenoid biosynthesis	57.69	15 of 26
66794	tyrosine metabolism	57.14	8 of 14
66794	glutathione metabolism	57.14	8 of 14
66794	degradation of pentoses	53.57	15 of 28
66794	degradation of sugar acids	52	13 of 25
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	cyclohexanol degradation	50	2 of 4
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	dTDPLrhamnose biosynthesis	50	4 of 8
66794	lysine metabolism	50	21 of 42
66794	glycine metabolism	50	5 of 10
66794	phosphatidylethanolamine bioynthesis	46.15	6 of 13
66794	phenylpropanoid biosynthesis	46.15	6 of 13
66794	sulfate reduction	46.15	6 of 13
66794	ascorbate metabolism	45.45	10 of 22
66794	cholesterol biosynthesis	45.45	5 of 11
66794	d-xylose degradation	45.45	5 of 11
66794	androgen and estrogen metabolism	43.75	7 of 16
66794	cardiolipin biosynthesis	42.86	3 of 7
66794	coenzyme M biosynthesis	40	4 of 10
66794	factor 420 biosynthesis	40	2 of 5
66794	4-hydroxyphenylacetate degradation	40	4 of 10
66794	metabolism of amino sugars and derivatives	40	2 of 5
66794	lipoate biosynthesis	40	2 of 5
66794	gallate degradation	40	2 of 5
66794	degradation of hexoses	38.89	7 of 18
66794	arachidonic acid metabolism	38.89	7 of 18
66794	carotenoid biosynthesis	36.36	8 of 22
66794	IAA biosynthesis	33.33	1 of 3
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	methane metabolism	33.33	1 of 3
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	3-phenylpropionate degradation	33.33	5 of 15
66794	sphingosine metabolism	33.33	2 of 6
66794	4-hydroxymandelate degradation	33.33	3 of 9
66794	enterobactin biosynthesis	33.33	1 of 3
66794	sulfoquinovose degradation	33.33	1 of 3
66794	nitrate assimilation	33.33	3 of 9
66794	urea cycle	30.77	4 of 13
66794	polyamine pathway	30.43	7 of 23
66794	benzoyl-CoA degradation	28.57	2 of 7
66794	catecholamine biosynthesis	25	1 of 4
66794	lactate fermentation	25	1 of 4
66794	phenol degradation	25	5 of 20
66794	carnitine metabolism	25	2 of 8
66794	toluene degradation	25	1 of 4

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
121399	-	-	+	+	-	+	-	-	-	-	-	+	-	-	-	-	+	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	#Fermented
#Environmental	#Microbial community	-
#Engineered	#Food production	#Beverage

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
17309	spoiled red wine	Dão region, Viseu	Portugal	PRT	Europe
121399	Food, Red wine	Dao	Portugal	PRT	Europe

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
17309	1	Risk group (German classification) According to TRBA 466
121399	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1419631v1 assembly for Acetobacter oeni DSM 23926	contig	GCA_014196315	304077.9	2829864053	304077	59.12
66792	ASM1151682v1 assembly for Acetobacter oeni LMG 21952	contig	GCA_011516825	304077.8	8114967252	304077	54.94
124043	ASM2599559v1 assembly for Acetobacter oeni LMG 21952	contig	GCA_025995595	1307912.3	8119182487	1307912	9.64

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Acetobacter oeni strain CECT 5830 16S ribosomal RNA gene, partial sequence	HQ398213	990	304077
20218	Acetobacter oeni strain LMG 21952 16S ribosomal RNA gene, partial sequence	JF793961	1351	304077
17309	Acetobacter oeni strain B13 16S ribosomal RNA gene, complete sequence	AY829472	1484	304077

GC content

@ref	GC-content (mol%)	Method
17309	58.1	high performance liquid chromatography (HPLC)
23234	58.10	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Acetobacter oeni LMG 21952
NCBI: GCA_011516825

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	96.50	no
125439	motility	BacteriaNetⓘ	yes	52.50	no
125439	gram_stain	BacteriaNetⓘ	negative	99.70	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.60	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Acetobacter oeni strain DSM 23926
PATRIC: 304077.9

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	97.83	yes
125438	anaerobic	anaerobicⓘ	no	94.37	no
125438	spore-forming	spore-formingⓘ	no	88.69	yes
125438	aerobic	aerobicⓘ	yes	83.75	no
125438	thermophilic	thermophileⓘ	no	98.18	yes
125438	flagellated	motile2+ⓘ	yes	68.89	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Development and Validation of a New Method for Detecting Acetic Bacteria in Wine.	Parra A, Ovejas A, Gonzalez-Arenzana L, Gutierrez AR, Lopez-Alfaro I.	Foods	10.3390/foods12203734	2023
	Molecular discrimination and identification of Acetobacter genus based on the partial heat shock protein 60 gene (hsp60) sequences	Huang C, Chang M, Huang L, Chua W.	Journal of the Science of Food and Agriculture.		2014
Phylogeny	Molecular discrimination and identification of Acetobacter genus based on the partial heat shock protein 60 gene (hsp60) sequences.	Huang CH, Chang MT, Huang L, Chua WS.	J Sci Food Agric	10.1002/jsfa.6231	2014
	Comprehensive deciphering prophages in genus Acetobacter on the ecology, genomic features, toxin-antitoxin system, and linkage with CRISPR-Cas system.	Qian C, Ma J, Liang J, Zhang L, Liang X.	Front Microbiol	10.3389/fmicb.2022.951030	2022
	Acetic Acid Bacteria in the Food Industry: Systematics, Characteristics and Applications.	Gomes RJ, Borges MF, Rosa MF, Castro-Gomez RJH, Spinosa WA.	Food Technol Biotechnol	10.17113/ftb.56.02.18.5593	2018
Phylogeny	Novel acetic acid bacteria from cider fermentations: Acetobacter conturbans sp. nov. and Acetobacter fallax sp. nov.	Sombolestani AS, Cleenwerck I, Cnockaert M, Borremans W, Wieme AD, De Vuyst L, Vandamme P.	Int J Syst Evol Microbiol	10.1099/ijsem.0.004511	2020
Phylogeny	Acetobacter sicerae sp. nov., isolated from cider and kefir, and identification of species of the genus Acetobacter by dnaK, groEL and rpoB sequence analysis.	Li L, Wieme A, Spitaels F, Balzarini T, Nunes OC, Manaia CM, Van Landschoot A, De Vuyst L, Cleenwerck I, Vandamme P.	Int J Syst Evol Microbiol	10.1099/ijs.0.058354-0	2014
Phylogeny	Acetobacter musti sp. nov., isolated from Bobal grape must.	Ferrer S, Manes-Lazaro R, Benavent-Gil Y, Yepez A, Pardo I	Int J Syst Evol Microbiol	10.1099/ijsem.0.000818	2015
Phylogeny	Acetobacter oeni sp. nov., isolated from spoiled red wine.	Silva LR, Cleenwerck I, Rivas R, Swings J, Trujillo ME, Willems A, Velazquez E	Int J Syst Evol Microbiol	10.1099/ijs.0.46000-0	2006

References

#17309	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23926
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#23234	Luis R. Silva Ilse Cleenwerck, Raúl Rivas, Jean Swings, Martha E. Trujillo, Anne Willems, Encarna Velázquez: Acetobacter oeni sp. nov., isolated from spoiled red wine. IJSEM 56: 21 - 24 2006 ( DOI 10.1099/ijs.0.46000-0 , PubMed 16403860 )
#37461	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#68382	Automatically annotated from API zym .
#121399	Collection of Institut Pasteur ; Curators of the CIP; CIP 109171
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Acetobacter oeni (DSM 23926, CECT 5830, LMG 21952)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Tolerance

Metabolite utilization

Metabolite production

Enzymes

Pathway annotation

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_014196315

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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