Brucella pituitosa (DSM 22207, CCUG 50899)

Name: Brucella pituitosa (DSM 22207, CCUG 50899)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1896

Type strain

Culture col. no. DSM 22207 CCUG 50899

NCBI tax ID(s) 571256

Special Collections DSMZ CCUG Literature strains

History DSMZ <- H.-J. Busse, Univ. Veterinary Medicine Vienna, Austria; CCUG 50899 <- H. C. Scholz <- E. Falsen

Links

Brucella pituitosa DSM 22207 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from industrial environment.

Gram-negative motile rod-shaped colony-forming aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 214848

CCUG 50899,DSM 22207

@ref 20215

Last LPSN update 2026-05-29 08:25:22

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Hyphomicrobiales

Family Bartonellaceae

Genus Brucella

Species Brucella pituitosa

Full scientific name Brucella pituitosa (Huber et al. 2010) Hördt et al. 2020

Synonyms (1)

Ochrobactrum pituitosum

BacDive ID	Other strains from **Brucella pituitosa** (1)	Type strain
176924	B. pituitosa ZK5343, NiuAA2, AA2, DSM 114565

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell shape	Confidence
23343	negative	1.5-2.5 µm	rod-shaped
125439	negative			99.053
125438	negative			100

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period
23343	1 mm	beige, translucent	circular	1 day

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
16203	R2A MEDIUM (DSMZ Medium 830)	Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
23343	Columbia agar		sheep blood
23343	MacConkey
23343	Nutrient agar (NA)
23343	PYE agar
23343	Reasoner's 2A agar (R2A)
23343	Trypticase Soy Agar (TSA)

Culture temp

@ref	Growth	Type	Temperature (°C)
16203	positive	growth	30
23343	positive	growth	25-30
23343	inconsistent	growth	20
23343	inconsistent	growth	35
23343	negative	growth	4
23343	negative	growth	>35
59044	positive	growth	30

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
23343	aerobe
59044	aerobe
125439	obligate aerobe	98.796

Spore formation

@ref	Spore formation	Confidence
23343
125439		99.806

Halophily

@ref	Salt	Growth	Tested relation	Concentration
23343	NaCl	positive	growth	6 %
23343	NaCl		growth	7 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23343	16763 ChEBI	2-oxobutanoate	-	assimilation
23343	16724 ChEBI	4-hydroxybutyrate	+	assimilation
23343	18101 ChEBI	4-hydroxyphenylacetic acid	-	assimilation
23343	91122 ChEBI	4-nitrophenyl alpha-D-glucopyranoside	+/-	hydrolysis
23343	90259 ChEBI	4-nitrophenyl beta-D-glucopyranoside	+/-	hydrolysis
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
23343	3122 ChEBI	bis-4-nitrophenyl phosphate	-	hydrolysis
23343	17057 ChEBI	cellobiose	-	builds acid from
23343	17057 ChEBI	cellobiose	+	assimilation
23343	16383 ChEBI	cis-aconitate	+	assimilation
23343	16947 ChEBI	citrate	+	assimilation
23343	18333 ChEBI	D-arabitol	+	assimilation
23343	8391 ChEBI	D-gluconate	-	assimilation
23343	17784 ChEBI	D-glucosaminic acid	+	assimilation
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
23343	16899 ChEBI	D-mannitol	+	assimilation
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
23343	17924 ChEBI	D-sorbitol	+	assimilation
23343	65327 ChEBI	D-xylose	+	builds acid from
23343	17113 ChEBI	erythritol	+/-	builds acid from
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
23343		gamma-L-glutamate-4-nitroanilide	+	hydrolysis
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
23343	28066 ChEBI	gentiobiose	+	assimilation
68369	24265 ChEBI	gluconate	-	assimilation	from API 20NE
23343	32323 ChEBI	glucuronamide	-	assimilation
23343	17754 ChEBI	glycerol	-	assimilation
23343	21217 ChEBI	L-alaninamide	-	assimilation
23343	30849 ChEBI	L-arabinose	+	builds acid from
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
23343	18287 ChEBI	L-fucose	+	assimilation
23343	15971 ChEBI	L-histidine	+	assimilation
23343	15603 ChEBI	L-leucine	+	assimilation
23343	62345 ChEBI	L-rhamnose	+	assimilation
23343	16857 ChEBI	L-threonine	-	assimilation
23343	17716 ChEBI	lactose	-	builds acid from
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
23343	68428 ChEBI	maltitol	+	assimilation
23343	17306 ChEBI	maltose	+	assimilation
68369	17306 ChEBI	maltose	+	assimilation	from API 20NE
23343	28053 ChEBI	melibiose	-	builds acid from
23343	17268 ChEBI	myo-inositol	-	builds acid from
23343	17268 ChEBI	myo-inositol	+	assimilation
23343	28037 ChEBI	N-acetylgalactosamine	+	assimilation
23343	506227 ChEBI	N-acetylglucosamine	-	assimilation
68369	59640 ChEBI	N-acetylglucosamine	+	assimilation	from API 20NE
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
23343	91050 ChEBI	p-nitrophenyl phenyl phosphonate	+	hydrolysis
23343	16634 ChEBI	raffinose	-	assimilation
23343	26546 ChEBI	rhamnose	-	builds acid from
23343	15963 ChEBI	ribitol	-	builds acid from
23343	15963 ChEBI	ribitol	+	assimilation
23343	41865 ChEBI	sebacic acid	-	assimilation
23343	143136 ChEBI	succinamate	-	assimilation
23343	17992 ChEBI	sucrose	+	assimilation
23343	15708 ChEBI	trans-aconitate	+	assimilation
23343	27082 ChEBI	trehalose	-	assimilation
23343	27082 ChEBI	trehalose	+	assimilation
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
23343	53426 ChEBI	tween 80	+	assimilation
68369	16199 ChEBI	urea	+	hydrolysis	from API 20NE
23343	16704 ChEBI	uridine	+	assimilation

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
23343	catalase	+	1.11.1.6
23343	cytochrome oxidase	+	1.9.3.1
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
68369	gelatinase	-		from API 20NE
68369	urease	+	3.5.1.5	from API 20NE

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
16203	+	-	-	-	+	-	-	-	+	+	+	-	+	+	-	+/-	-	+	+	-	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Engineered	#Industrial

Isolation

@ref	Sample type	Sampling date	Country	Country ISO 3 Code	Continent
16203	industrial environment		Sweden	SWE	Europe
59044	Industry,environment	2005-04-01	Sweden	SWE	Europe

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
16203	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM880170v1 assembly for Brucella pituitosa CCUG 50899	contig	GCA_008801705	571256.25		571256	49.08
124043	ASM4192998v1 assembly for Brucella pituitosa DSM 22207	scaffold	GCA_041929985			571256	31.7
66792	ASM304968v2 assembly for Brucella pituitosa CCUG 50899	contig	GCA_003049685	571256.19	8116734741	571256	24.04

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
16203	Ochrobactrum pituitosum partial 16S rRNA gene, strain CCUG 50899	AM490609	1429		571256

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM880170v1 assembly for Brucella pituitosa CCUG 50899
BV-BRC: 571256.25

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.80	no
125439	gram_stain	BacteriaNetⓘ	negative	99.05	no
125439	motility	BacteriaNetⓘ	yes	69.21	no
125439	spore_formation	BacteriaNetⓘ	no	99.81	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Ochrobactrum pituitosum strain CCUG 50899
PATRIC: 571256.25

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	100.00	yes
125438	anaerobic	anaerobicⓘ	no	94.94	no
125438	spore-forming	spore-formingⓘ	no	89.01	yes
125438	aerobic	aerobicⓘ	yes	83.45	no
125438	thermophilic	thermophileⓘ	no	97.62	yes
125438	flagellated	motile2+ⓘ	yes	67.76	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Crown Gall Induced by a Natural Isolate of Brucella (Ochrobactrum) pseudogrignonense Containing a Tumor-Inducing Plasmid.	Hooykaas MJG, Hooykaas PJJ.	Microorganisms	10.3390/microorganisms13010102	2025
Genetics	Analysis of 1,000+ Type-Strain Genomes Substantially Improves Taxonomic Classification of Alphaproteobacteria.	Hordt A, Lopez MG, Meier-Kolthoff JP, Schleuning M, Weinhold LM, Tindall BJ, Gronow S, Kyrpides NC, Woyke T, Goker M.	Front Microbiol	10.3389/fmicb.2020.00468	2020
Enzymology	Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and Identification of Potential Bacterial Chassis for Studying Plant-Microbe Interactions.	van Schaik J, Li Z, Cheadle J, Crook N.	ACS Synth Biol	10.1021/acssynbio.3c00371	2023
	Evaluation of ready-to-use freezer stocks of a synthetic microbial community for maize root colonization.	Parnell JJ, Vintila S, Tang C, Wagner MR, Kleiner M.	Microbiol Spectr	10.1128/spectrum.02401-23	2024
Genetics	Core Genome Multilocus Sequence Typing Scheme for Improved Characterization and Epidemiological Surveillance of Pathogenic Brucella.	Abdel-Glil MY, Thomas P, Brandt C, Melzer F, Subbaiyan A, Chaudhuri P, Harmsen D, Jolley KA, Janowicz A, Garofolo G, Neubauer H, Pletz MW.	J Clin Microbiol	10.1128/jcm.00311-22	2022
Phylogeny	Ochrobactrum daejeonense sp. nov., a nitrate-reducing bacterium isolated from sludge of a leachate treatment plant.	Woo SG, Ten LN, Park J, Lee M	Int J Syst Evol Microbiol	10.1099/ijs.0.025510-0	2010
Phylogeny	Ochrobactrum pituitosum sp. nov., isolated from an industrial environment.	Huber B, Scholz HC, Kampfer P, Falsen E, Langer S, Busse HJ	Int J Syst Evol Microbiol	10.1099/ijs.0.011668-0	2009

References

#16203	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22207
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#23343	Birgit Huber, Holger C. Scholz, Peter Kämpfer, Enevold Falsen, Stefan Langer, Hans-Jürgen Busse: Ochrobactrum pituitosum sp. nov., isolated from an industrial environment. IJSEM 60: 321 - 326 2010 ( DOI 10.1099/ijs.0.011668-0 , PubMed 19651740 )
#59044	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 50899
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68369	Automatically annotated from API 20NE .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Brucella pituitosa (DSM 22207, CCUG 50899)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_008801705

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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