Brucella pseudogrignonensis (DSM 22354, CCUG 30717, CIP 109451)

Name: Brucella pseudogrignonensis (DSM 22354, CCUG 30717, CIP 109451)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1892

Type strain

Culture col. no. DSM 22354 CCUG 30717 CIP 109451

NCBI tax ID(s) 419475

Special Collections DSMZ CCUG CIP Literature strains

History <- CIP <- CCUG <- ? 2007, CCUG

Links

Brucella pseudogrignonensis DSM 22354 is a mesophilic, Gram-negative, rod-shaped prokaryote that forms circular colonies and was isolated from blood of a 28-year-old man.

Gram-negative rod-shaped colony-forming mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 57055

CCUG 30717,CIP 109451,DSM 22354

@ref 20215

Last LPSN update 2025-12-16 09:47:12

Domain Pseudomonadati

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Hyphomicrobiales

Family Brucellaceae

Genus Brucella

Species Brucella pseudogrignonensis

Full scientific name Brucella pseudogrignonensis (Kämpfer et al. 2007) Hördt et al. 2020

Synonyms (1)

Ochrobactrum pseudogrignonense

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell shape	Confidence
23341	negative	2 µm	rod-shaped
125438	negative			98.5
125439	negative			99.7

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period
23341	2 mm	beige, translucent	circular	1 day

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
16310	CASO AGAR (MERCK 105458) (DSMZ Medium 220)	Medium recipe at MediaDive	Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
23341	MacConkey
23341	Nutrient agar (NA)
23341	PYE agar
23341	Reasoner's 2A agar (R2A)
23341	Trypticase Soy Agar (TSA)
37560	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
16310	positive	growth	30	mesophilic
23341	positive	growth	28-30	mesophilic
23341	positive	growth	4	psychrophilic
23341	negative	growth	42
37560	positive	growth	30	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	97.7

Spore formation

@ref	Spore formation	Confidence
23341
125439		99.2

Halophily

@ref	Salt	Growth	Tested relation	Concentration
23341	NaCl	positive	growth	5 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23341	37054 ChEBI	3-hydroxybutyrate	+	assimilation
23341	17879 ChEBI	4-hydroxybenzoate	-	assimilation
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
23341	16958 ChEBI	beta-alanine	+	assimilation
23341	17057 ChEBI	cellobiose	-	assimilation
23341	16383 ChEBI	cis-aconitate	+	assimilation
23341	16947 ChEBI	citrate	+	assimilation
23341	15824 ChEBI	D-fructose	+	assimilation
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
23341	17924 ChEBI	D-sorbitol	+	assimilation
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
23341	16865 ChEBI	gamma-aminobutyric acid	+	assimilation
23341		gamma-L-glutamate-4-nitroanilide	-	hydrolysis
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	-	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
23341	29991 ChEBI	L-aspartate	+	assimilation
23341	15971 ChEBI	L-histidine	+	assimilation
23341	15603 ChEBI	L-leucine	+	assimilation
23341		L-proline-4-nitroanilide	+	hydrolysis
23341	62345 ChEBI	L-rhamnose	+	assimilation
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
23341	68428 ChEBI	maltitol	-	assimilation
23341	17306 ChEBI	maltose	-	assimilation
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
23341	17268 ChEBI	myo-inositol	+	assimilation
23341	28037 ChEBI	N-acetylgalactosamine	+	assimilation
23341	506227 ChEBI	N-acetylglucosamine	-	assimilation
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
23341	91050 ChEBI	p-nitrophenyl phenyl phosphonate	+/-	hydrolysis
23341	15963 ChEBI	ribitol	+	assimilation
23341	9300 ChEBI	suberic acid	-	assimilation
23341	17992 ChEBI	sucrose	+	assimilation
23341	15708 ChEBI	trans-aconitate	+	assimilation
23341	27082 ChEBI	trehalose	+	assimilation
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
23341	cytochrome oxidase	+	1.9.3.1
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
16310	cytochrome-c oxidase	+	1.9.3.1
68369	gelatinase	-		from API 20NE
68369	urease	-	3.5.1.5	from API 20NE

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
16310	+	-	-	-	-	-	-	-	+	+	+	+/-	+/-	-	-	-	-	+	+	-	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Infection	#Patient	-
#Host Body Product	#Fluids	#Blood
#Host	#Human	#Male

Isolation

@ref	Sample type	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent
16310	blood of a 28-year-old man		Göteborg	Sweden	SWE	Europe
50239	Human blood,28-yr-old man	1992-10-28	Göteborg	Sweden	SWE	Europe

Taxonmaps

69479

Global distribution of 16S sequence AM422371 (>99% sequence identity) for Ochrobactrum from Microbeatlas

Aquatic Samples	738
Soil Samples	503
Animal Samples	2153
Plant Samples	1125
Total Samples	4519

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Biosafety level	Biosafety level comment
16310	yes, in single cases	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM225252v1 assembly for Brucella pseudogrignonensis CCUG 30717	contig	GCA_002252525	419475.13	2854890933	419475	55.73
124043	ASM4192994v1 assembly for Brucella pseudogrignonensis CIP 109451	scaffold	GCA_041929945			419475	26.37
66792	ASM1239520v1 assembly for Brucella pseudogrignonensis CIP 109451	contig	GCA_012395205	419475.20		419475

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
16310	Ochrobactrum pseudogrignonense partial 16S rRNA gene, type strain CCUG 30717T	AM422371	1387		419475

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Brucella pseudogrignonensis CCUG 30717
NCBI: GCA_002252525

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	99.20	no
125439	motility	BacteriaNetⓘ	yes	77.30	no
125439	gram_stain	BacteriaNetⓘ	negative	99.70	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	97.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Ochrobactrum pseudogrignonense strain CCUG 30717
PATRIC: 419475.13

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.50	no
125438	anaerobic	anaerobicⓘ	no	96.02	no
125438	spore-forming	spore-formingⓘ	no	89.05	no
125438	aerobic	aerobicⓘ	yes	84.18	no
125438	thermophilic	thermophileⓘ	no	99.49	yes
125438	flagellated	motile2+ⓘ	yes	66.45	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Analysis of 1,000+ Type-Strain Genomes Substantially Improves Taxonomic Classification of Alphaproteobacteria.	Hordt A, Lopez MG, Meier-Kolthoff JP, Schleuning M, Weinhold LM, Tindall BJ, Gronow S, Kyrpides NC, Woyke T, Goker M.	Front Microbiol	10.3389/fmicb.2020.00468	2020
	Crown Gall Induced by a Natural Isolate of Brucella (Ochrobactrum) pseudogrignonense Containing a Tumor-Inducing Plasmid.	Hooykaas MJG, Hooykaas PJJ.	Microorganisms	10.3390/microorganisms13010102	2025
Phylogeny	Genomics of Ochrobactrum pseudogrignonense (newly named Brucella pseudogrignonensis) reveals a new bla_OXA subgroup.	Li SY, Huang YE, Chen JY, Lai CH, Mao YC, Huang YT, Liu PY.	Microb Genom	10.1099/mgen.0.000626	2021
Phylogeny	Ochrobactrum haematophilum sp. nov. and Ochrobactrum pseudogrignonense sp. nov., isolated from human clinical specimens.	Kampfer P, Scholz HC, Huber B, Falsen E, Busse HJ	Int J Syst Evol Microbiol	10.1099/ijs.0.65066-0	2007

References

#16310	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22354
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#23341	Peter Kämpfer, Holger C. Scholz, Birgit Huber, Enevold Falsen, Hans-Jürgen Busse: Ochrobactrum haematophilum sp. nov. and Ochrobactrum pseudogrignonense sp. nov., isolated from human clinical specimens. IJSEM 57: 2513 - 2518 2007 ( DOI 10.1099/ijs.0.65066-0 , PubMed 17978211 )
#37560	; Curators of the CIP;
#50239	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 30717
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68369	Automatically annotated from API 20NE .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Brucella pseudogrignonensis (DSM 22354, CCUG 30717, CIP 109451)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002252525

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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