Name: Brucella intermedia DSM 17986
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1888

Type strain:

Species: Brucella intermedia

Culture col. no.: DSM 17986, CIP 105838, LMG 3301, NCTC 12171, CCUG 24694, CNS 2-75

Strain history: <- CIP; CIP 105838 <- I. Moriyón, Univ. Navarra, Spain; CNS 2-75

NCBI tax ID(s): 641118 (strain), 94625 (species)

SI-ID 3682

LMG 3301, NCTC 12171, CCUG 24694, CIP 105838, IFO 15820, CCM 7179, NBRC 15820, BCRC 17247, CCRC 17247, DSM 17986, VTT E-991166

Section

Brucella intermedia DSM 17986 is a mesophilic, motile, rod-shaped bacterium that was isolated from human blood.

motile
rod-shaped
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Brucellaceae*
	Genus	*Brucella*
	Species	**Brucella intermedia**
	Full Scientific Name (LPSN)	Brucella intermedia (Velasco et al. 1998) Hördt et al. 2020

Synonym

Ochrobactrum intermedium

Information on morphological and physiological properties Morphology

[Ref.: #23338]	Cell shape	rod-shaped

[Ref.: #23338]	Motility	yes

[Ref.: #23338]	Flagellum arrangement	lophotrichous

[Ref.: #23338]	Colony size	1 mm
[Ref.: #23338]	Colony shape	circular
[Ref.: #23338]	Incubation period	1 day
[Ref.: #23338]	Cultivation medium used	Nutrient agar

[Ref.: #7318]	Incubation period	1-2 days

Information on culture and growth conditions Culture and growth conditions

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Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

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Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

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Culture medium growth

[Ref.: #7318]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7318]

Culture medium composition

expand / minimize

[Ref.: #23338]

Culture medium

Nutrient agar (NA)

[Ref.: #23338]

Culture medium growth

[Ref.: #37230]

Culture medium

MEDIUM 3 - Columbia agar

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Culture medium growth

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Culture medium composition

expand / minimize

		Temperature
[Ref.: #7318]	growth	30 °C
[Ref.: #23338]	growth	37 °C
[Ref.: #23338]	growth	18-22 °C
[Ref.: #37230]	growth	30 °C

[Ref.: #7318]	Temperature range	mesophilic
[Ref.: #23338]	Temperature range	mesophilic
[Ref.: #23338]	Temperature range	psychrophilic
[Ref.: #37230]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23338]	4-coumarate ChEBI	-	assimilation
[Ref.: #23338]	4-nitrophenyl beta-D-galactopyranoside hydrolysate	-	hydrolysis
[Ref.: #23338]	4-nitrophenyl beta-D-glucopyranoside ChEBI	+	hydrolysis
[Ref.: #23338]	4-nitrophenyl beta-D-glucuronide ChEBI	-	hydrolysis
[Ref.: #23338]	4-nitrophenyl beta-D-xylopyranoside ChEBI	-	hydrolysis
[Ref.: #23338]	acetamide ChEBI	-
[Ref.: #23338]	adipate ChEBI	-	assimilation
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #23338]	arabinose ChEBI	-	assimilation
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #23338]	bis-4-nitrophenyl phosphate ChEBI	-	hydrolysis
[Ref.: #23338]	bis-4-nitrophenyl phosphate ChEBI	+	hydrolysis
[Ref.: #23338]	citrate ChEBI	+	assimilation
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #23338]	esculin ChEBI	-	hydrolysis
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #23338]	glucose ChEBI	-	assimilation
[Ref.: #23338]	glycerol ChEBI	+	assimilation
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #23338]	L-proline-4-nitroanilide	-	hydrolysis
[Ref.: #23338]	lactose ChEBI	-	assimilation
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #23338]	malonate ChEBI	-
[Ref.: #23338]	maltose ChEBI	-	assimilation
[Ref.: #23338]	maltose ChEBI	+	assimilation
[Ref.: #68369]	maltose ChEBI	+	assimilation	* from API 20NE
[Ref.: #23338]	mannitol ChEBI	-	assimilation
[Ref.: #23338]	melibiose ChEBI	-	assimilation
[Ref.: #23338]	myo-inositol ChEBI	-	assimilation
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #23338]	nitrate ChEBI	+	reduction
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #23338]	O-nitrophenyl-beta-D-galactopyranosid	-	hydrolysis
[Ref.: #23338]	p-nitrophenylphosphocholine ChEBI	-	hydrolysis
[Ref.: #23338]	phenylacetate ChEBI	-	assimilation
[Ref.: #23338]	raffinose ChEBI	-	assimilation
[Ref.: #23338]	rhamnose ChEBI	-	assimilation
[Ref.: #23338]	sorbitol ChEBI	-	assimilation
[Ref.: #23338]	sucrose ChEBI	-	assimilation
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic resistance
[Ref.: #23338]		colistin ChEBI	yes

	Metabolite	Production
[Ref.: #23338]	hydrogen sulfide ChEBI	yes
[Ref.: #23338]	indole ChEBI	no
[Ref.: #68369]	indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #23338]	arginine dihydrolase	-	3.5.3.6
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #23338]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #23338]	gelatinase	-
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #23338]	lysine decarboxylase	-	4.1.1.18
[Ref.: #23338]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #7318]	24900	-	-	-	-	-	-	-	-	+	+	+	+	+	+	+/-	+	-	+	+	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7318]	Sample type/isolated from	human blood
[Ref.: #7318]	Country	France
[Ref.: #7318]	Country ISO 3 Code	FRA
[Ref.: #7318]	Continent	Europe

[Ref.: #48013]	Sample type/isolated from	Human blood
[Ref.: #48013]	Country	France
[Ref.: #48013]	Country ISO 3 Code	FRA
[Ref.: #48013]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body Product	#Fluids	#Blood

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AM114411 (>99% sequence identity) for Brucella from Microbeatlas

Aquatic Samples	171
Soil Samples	255
Animal Samples	403
Plant Samples	72
Total Samples	901

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7318]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7318]	Ochrobactrum intermedium gene for 16S rRNA, partial sequence, strain: NBRC 15820	AB680967	1410	ENA	94625 tax ID
[Ref.: #7318]	Ochrobactrum intermedium partial 16S rRNA gene, type strain CCUG 24694T	AM114411	1390	ENA	94625 tax ID
[Ref.: #23338]	Ochrobactrum intermedium LMG 3301 16S ribosomal RNA gene, partial sequence	U70978	1454	GenBank	641118 tax ID
[Ref.: #20218]	Ochrobactrum intermedium 16S rRNA gene (partial), 23S rRNA gene (partial), tRNA-Ile gene, tRNA-Ala gene and ITS1, type strain LMG 3301, allele A	AJ867340	913	ENA	94625 tax ID	*
[Ref.: #20218]	Ochrobactrum intermedium 16S rRNA gene (partial), 23S rRNA gene (partial), tRNA-Ile gene, tRNA-Ala gene and ITS1, type strain LMG 3301, allele B	AJ867341	915	ENA	94625 tax ID	*
[Ref.: #20218]	Ochrobactrum intermedium 16S rRNA gene (partial), 23S rRNA gene (partial), tRNA-Ile gene, tRNA-Ala gene and ITS1, type strain LMG 3301, allele C	AJ867342	915	ENA	94625 tax ID	*
[Ref.: #20218]	Ochrobactrum intermedium 16S rRNA gene (partial), 23S rRNA gene (partial), tRNA-Ile gene, tRNA-Ala gene and ITS1, type strain LMG 3301, allele D	AJ867343	936	ENA	94625 tax ID	*
[Ref.: #20218]	Ochrobactrum intermedium 16S rRNA gene (partial), 23S rRNA gene (partial), tRNA-Ile gene, tRNA-Ala gene and ITS1, type strain LMG 3301, allele E	AJ867344	985	ENA	94625 tax ID	*
[Ref.: #20218]	Ochrobactrum intermedium partial 16S rRNA gene, strain LMG 3301T	AM490623	1390	ENA	641118 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Brucella intermedia NCTC12171	GCA_900454225	contig	GenBank	94625 tax ID	*
[Ref.: #66792]	Brucella intermedia LMG 3301	GCA_000182645	scaffold	GenBank	641118 tax ID	*
[Ref.: #66792]	Brucella intermedia CIP 105838	GCA_012103055	contig	GenBank	94625 tax ID	*
[Ref.: #66792]	Ochrobactrum intermedium LMG 3301	641118.3	wgs	PATRIC	641118 tax ID	*
[Ref.: #66792]	Ochrobactrum intermedium strain CIP 105838	94625.26	wgs	PATRIC	94625 tax ID	*
[Ref.: #66792]	Ochrobactrum intermedium strain NCTC12171	94625.13	wgs	PATRIC	94625 tax ID	*

[Ref.: #23338]

GC-content

57.7-58.3 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Brucella intermedia LMG 3301 LMG 3301 NCBI: GCA_000182645.1
[Ref.: #69480]	motile	yes	81.775	yes
[Ref.: #69480]	flagellated	no	85.664	yes
[Ref.: #69480]	gram-positive	no	98.293	no
[Ref.: #69480]	anaerobic	no	97.759	no
[Ref.: #69480]	aerobic	yes	94.057	no
[Ref.: #69480]	halophile	no	93.299	no
[Ref.: #69480]	spore-forming	no	95.926	no
[Ref.: #69480]	thermophile	no	98.421	no
[Ref.: #69480]	glucose-util	yes	92.206	no
[Ref.: #69480]	glucose-ferment	no	91.194	no

Availability in culture collections External links

[Ref.: #7318]

Culture collection no.

DSM 17986, CIP 105838, LMG 3301, NCTC 12171, CCUG 24694, CNS 2-75

[Ref.: #71532]

SI-ID 3682

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Pathogenicity	Prevalence, Host Range, and Comparative Genomic Analysis of Temperate Ochrobactrum Phages.	Jackel C, Hertwig S, Scholz HC, Nockler K, Reetz J, Hammerl JA	Front Microbiol	10.3389/fmicb.2017.01207	2017	*
Phylogeny	Isolation and characterization of a crude oil degrading bacteria from formation water: comparative genomic analysis of environmental Ochrobactrum intermedium isolate versus clinical strains.	Chai LJ, Jiang XW, Zhang F, Zheng BW, Shu FC, Wang ZL, Cui QF, Dong HP, Zhang ZZ, Hou DJ, She YH	J Zhejiang Univ Sci B	10.1631/jzus.B1500029	2015	*
Biotechnology	Acquired genetic mechanisms of a multiresistant bacterium isolated from a treatment plant receiving wastewater from antibiotic production.	Johnning A, Moore ER, Svensson-Stadler L, Shouche YS, Larsson DG, Kristiansson E	Appl Environ Microbiol	10.1128/AEM.02141-13	2013	*
Phylogeny	Ochrobactrum pecoris sp. nov., isolated from farm animals.	Kampfer P, Huber B, Busse HJ, Scholz HC, Tomaso H, Hotzel H, Melzer F	Int J Syst Evol Microbiol	10.1099/ijs.0.027631-0	2010	*
Phylogeny	Ochrobactrum ciceri sp. nov., isolated from nodules of Cicer arietinum.	Imran A, Hafeez FY, Fruhling A, Schumann P, Malik KA, Stackebrandt E	Int J Syst Evol Microbiol	10.1099/ijs.0.013987-0	2009	*
Phylogeny	Isolation of urease-positive Ochrobactrum intermedium in the stomach of a non-ulcer dyspeptic patient from north India.	Dharne MS, Misra SP, Misra V, Dwivedi M, Patole MS, Shouche YS	J Microbiol Immunol Infect		2008	*
Phylogeny	Comparative sequence analysis of the internal transcribed spacer 1 of Ochrobactrum species.	Lebuhn M, Bathe S, Achouak W, Hartmann A, Heulin T, Schloter M	Syst Appl Microbiol	10.1016/j.syapm.2005.11.003	2005	*
Phylogeny	Evaluation of the relatedness of Brucella spp. and Ochrobactrum anthropi and description of Ochrobactrum intermedium sp. nov., a new species with a closer relationship to Brucella spp.	Velasco J, Romero C, Lopez-Goni I, Leiva J, Diaz R, Moriyon I	Int J Syst Bacteriol	10.1099/00207713-48-3-759	1998	*
	Draft genome of Ochrobactrum intermedium strain M86 isolated from non-ulcer dyspeptic individual from India.	Kulkarni G, Dhotre D, Dharne M, Shetty S, Chowdhury S, Misra V, Misra S, Patole M, Shouche Y	Gut Pathog	10.1186/1757-4749-5-7	2013	*

References

#7318

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17986

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23338

Julián Velasco, Conchi Romero, Ignacio López-Goni, José Leiva, Ramón Díaz, Ignacio Moriyón: Evaluation of the relatedness of Brucella spp. and Ochrobactrum anthropi and description of Ochrobactrum intermedium sp. nov., a new species with a closer relationship to Brucella spp.. IJSEM 48: 759 - 768 1998 ( DOI 10.1099/00207713-48-3-759 , PubMed 9734029 )

#37230 ; Curators of the CIP;
#48013 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 24694

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#71532

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID3682.1 )

* These data were automatically processed and therefore are not curated

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Brucella intermedia LMG 3301 LMG 3301

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