Name: Brucella tritici SCII24
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1884

Type strain:

Species: Brucella tritici

Strain Designation: SCII24

Culture col. no.: DSM 13340, NBRC 102585, CCUG 47104, LMG 18957, UCCCB44

Strain history: Lebuhn M. DSV-DEVM and GSF - NationalResearch Center forEnvironment and Health

NCBI tax ID(s): 94626 (species)

SI-ID 13104

LMG 18957, DSM 13340, CCM 7181, BCRC 17250, CCRC 17250, CCUG 47104, NBRC 102585, CIP 107374

Other strains from Brucella tritici

B. tritici LAIII106, DSM 13341

Section

Brucella tritici SCII24 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from rhizosphere of wheat.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Brucellaceae*
	Genus	*Brucella*
	Species	**Brucella tritici**
	Full Scientific Name (LPSN)	Brucella tritici (Lebuhn et al. 2000) Hördt et al. 2020

Synonym

Ochrobactrum tritici

Information on morphological and physiological properties Morphology

[Ref.: #23335]	Gram stain	negative

[Ref.: #23335]	Cell length	0.6-1.4 µm

[Ref.: #23335]	Cell width	0.4-0.6 µm

[Ref.: #23335]	Cell shape	rod-shaped

[Ref.: #23335]	Motility	yes

[Ref.: #23335]	Flagellum arrangement	peritrichous

[Ref.: #23335]	Colony color	opaque
[Ref.: #23335]	Cultivation medium used	NA

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5003]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #5003]

Culture medium growth

[Ref.: #5003]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5003]

Culture medium composition

expand / minimize

[Ref.: #23335]

Culture medium

Nutrient agar (NA)

[Ref.: #23335]

Culture medium growth

[Ref.: #33780]

Culture medium

MEDIUM 328- for nutrient agar

[Ref.: #33780]

Culture medium growth

[Ref.: #33780]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #5003]	growth	30 °C
[Ref.: #23335]	growth	4-40 °C
[Ref.: #23335]	optimum	30 °C
[Ref.: #33780]	growth	30 °C
[Ref.: #57511]	growth	30 °C
[Ref.: #67772]	minimum	4 °C
[Ref.: #67772]	optimum	30 °C
[Ref.: #67772]	maximum	40 °C

[Ref.: #5003]	Temperature range	mesophilic
[Ref.: #23335]	Temperature range	mesophilic
[Ref.: #33780]	Temperature range	mesophilic
[Ref.: #57511]	Temperature range	mesophilic
[Ref.: #67772]	Temperature range	psychrophilic
[Ref.: #67772]	Temperature range	mesophilic
[Ref.: #67772]	Temperature range	thermophilic

		pH
[Ref.: #67772]	maximum	9.0
[Ref.: #67772]	minimum	3.0
[Ref.: #23335]	optimum	6.0-7.0
[Ref.: #67772]	optimum	6.0-7.0
[Ref.: #23335]	growth	3.0-9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23335]	Oxygen tolerance	aerobe
[Ref.: #57511]	Oxygen tolerance	aerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23335]	(-)-quinic acid ChEBI	-	assimilation
[Ref.: #23335]	2-hydroxybutyrate ChEBI	-	assimilation
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #23335]	arabinose ChEBI	-	assimilation
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #23335]	cellobiose ChEBI	-	assimilation
[Ref.: #23335]	citrate ChEBI	+/-	assimilation
[Ref.: #23335]	D-glucosaminic acid ChEBI	+	assimilation
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #23335]	D-trehalose ChEBI	+	assimilation
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #23335]	DL-carnitine ChEBI	+/-	assimilation
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #23335]	gentiobiose ChEBI	-	assimilation
[Ref.: #23335]	gluconate ChEBI	+	assimilation
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #23335]	glycerol ChEBI	-	assimilation
[Ref.: #23335]	glycerol 1-phosphate ChEBI	+/-	assimilation
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #23335]	malonate ChEBI	-	assimilation
[Ref.: #23335]	maltose ChEBI	+	assimilation
[Ref.: #68369]	maltose ChEBI	+	assimilation	* from API 20NE
[Ref.: #23335]	mannose ChEBI	-	assimilation
[Ref.: #23335]	melibiose ChEBI	-	assimilation
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #23335]	ribitol ChEBI	+	assimilation
[Ref.: #23335]	sebacic acid ChEBI	+	assimilation
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #23335]	uridine ChEBI	+/-	assimilation
[Ref.: #23335]	xylitol ChEBI	-	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Antibiotic intermediate	Antibiotic resistance
[Ref.: #23335]	chloramphenicol ChEBI			yes
[Ref.: #23335]	colistin ChEBI		yes
[Ref.: #23335]	colistin ChEBI	yes
[Ref.: #23335]	polymyxin b ChEBI	yes

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #23335]	catalase	+	1.11.1.6
[Ref.: #23335]	cytochrome oxidase		1.9.3.1
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68369]	urease	+	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #5003]	23709	+	-	-	-	+	-	-	-	+	+	+	+/-	+	+	+	+	-	+	+	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5003]	Sample type/isolated from	rhizosphere of wheat
[Ref.: #5003]	Country	France
[Ref.: #5003]	Country ISO 3 Code	FRA
[Ref.: #5003]	Continent	Europe

[Ref.: #23335]	Sample type/isolated from	rhizoplane of wheat (Triticum aestivum)

[Ref.: #57511]	Sample type/isolated from	Wheat rhizoplane
[Ref.: #57511]	Sampling date	1997
[Ref.: #57511]	Geographic location (country and/or sea, region)	Grignon
[Ref.: #57511]	Country	France
[Ref.: #57511]	Country ISO 3 Code	FRA
[Ref.: #57511]	Continent	Europe

[Ref.: #67772]	Sample type/isolated from	Wheat rhizoplane
[Ref.: #67772]	Geographic location (country and/or sea, region)	Auvergne-Rhône-Alpes, Grignon
[Ref.: #67772]	Country	France
[Ref.: #67772]	Country ISO 3 Code	FRA
[Ref.: #67772]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host Body-Site	#Plant	#Rhizoplane
#Host	#Plants	#Herbaceous plants (Grass,Crops)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB681865 (>99% sequence identity) for Ochrobactrum from Microbeatlas

Aquatic Samples	339
Soil Samples	432
Animal Samples	1469
Plant Samples	154
Total Samples	2394

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5003]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #67772]	Biosafety level	1

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #23335]	Ochrobactrum tritici partial 16S rRNA gene, strain SCII24	AJ242584	1398	GenBank	94626 tax ID
[Ref.: #20218]	Ochrobactrum tritici gene for 16S rRNA, partial sequence, strain: NBRC 102585	AB681865	1408	ENA	94626 tax ID	*
[Ref.: #20218]	Ochrobactrum tritici 16S rRNA gene (partial), 23S rRNA gene (partial), tRNA-Ile gene, tRNA-Ala gene and ITS1, type strain SCII24, allele B	AJ867329	964	ENA	94626 tax ID	*
[Ref.: #20218]	Ochrobactrum tritici 16S rRNA gene (partial), 23S rRNA gene (partial), tRNA-Ile gene, tRNA-Ala gene and ITS1, type strain SCII24, allele A	AJ867330	934	ENA	94626 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67772]	Brucella tritici LMG 18957	GCA_008932295	contig	GenBank	94626 tax ID
[Ref.: #66792]	Brucella tritici DSM 13340	GCA_012395245	contig	GenBank	94626 tax ID	*
[Ref.: #66792]	Ochrobactrum tritici strain DSM 13340	94626.11	wgs	PATRIC	94626 tax ID	*
[Ref.: #66792]	Ochrobactrum tritici strain LMG 18957	94626.9	wgs	PATRIC	94626 tax ID	*

[Ref.: #23335]

GC-content

59.00 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Ochrobactrum tritici strain DSM 13340 PATRIC: 94626.11
[Ref.: #69480]	motile	yes	83.455	yes
[Ref.: #69480]	flagellated	no	87.191	no
[Ref.: #69480]	gram-positive	no	98.364	no
[Ref.: #69480]	anaerobic	no	97.425	no
[Ref.: #69480]	halophile	no	92.049	no
[Ref.: #69480]	spore-forming	no	96.741	no
[Ref.: #69480]	thermophile	no	98.905	no
[Ref.: #69480]	glucose-util	yes	92.639	yes
[Ref.: #69480]	aerobic	yes	91.457	yes
[Ref.: #69480]	glucose-ferment	no	90.876	yes

Availability in culture collections External links

[Ref.: #5003]

Culture collection no.

DSM 13340, NBRC 102585, CCUG 47104, LMG 18957, UCCCB44

[Ref.: #71528]

SI-ID 13104

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Arsenic accumulation by a rhizosphere bacterial strain Ochrobactrum tritici reduces rice plant arsenic levels.	Moens M, Branco R, Morais PV	World J Microbiol Biotechnol	10.1007/s11274-020-2800-0	2020	*
Metabolism	Immobilization of Ochrobactrum tritici As5 on PTFE thin films for arsenite biofiltration.	Branco R, Sousa T, Piedade AP, Morais PV	Chemosphere	10.1016/j.chemosphere.2015.12.025	2015	*
Metabolism	Hyper Accumulation of Arsenic in Mutants of Ochrobactrum tritici Silenced for Arsenite Efflux Pumps.	Sousa T, Branco R, Piedade AP, Morais PV	PLoS One	10.1371/journal.pone.0131317	2015	*
Metabolism	Identification of an aox system that requires cytochrome c in the highly arsenic-resistant bacterium Ochrobactrum tritici SCII24.	Branco R, Francisco R, Chung AP, Morais PV	Appl Environ Microbiol	10.1128/AEM.02798-08	2009	*
Genetics	Sequencing and expression of two arsenic resistance operons with different functions in the highly arsenic-resistant strain Ochrobactrum tritici SCII24T.	Branco R, Chung AP, Morais PV	BMC Microbiol	10.1186/1471-2180-8-95	2008	*
Phylogeny	Ochrobactrum cytisi sp. nov., isolated from nodules of Cytisus scoparius in Spain.	Zurdo-Pineiro JL, Rivas R, Trujillo ME, Vizcaino N, Carrasco JA, Chamber M, Palomares A, Mateos PF, Martinez-Molina E, Velazquez E	Int J Syst Evol Microbiol	10.1099/ijs.0.64613-0	2007	*
Phylogeny	Taxonomic characterization of Ochrobactrum sp. isolates from soil samples and wheat roots, and description of Ochrobactrum tritici sp. nov. and Ochrobactrum grignonense sp. nov.	Lebuhn M, Achouak W, Schloter M, Berge O, Meier H, Barakat M, Hartmann A, Heulin T	Int J Syst Evol Microbiol	10.1099/00207713-50-6-2207	2000	*

References

#5003

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13340

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23335

M Lebuhn, W Achouak, M Schloter, O Berge, H Meier, M Barakat, A Hartmann, T Heulin: Taxonomic characterization of Ochrobactrum sp. isolates from soil samples and wheat roots, and description of Ochrobactrum tritici sp. nov. and Ochrobactrum grignonense sp. nov.. IJSEM 50: 2207 - 2223 2000 ( DOI 10.1099/00207713-50-6-2207 , PubMed 11155998 )

#33780 ; Curators of the CIP;
#57511 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 47104

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67772 The University of Coimbra Bacteria Culture Collection (UCCCB) ; Curators of the UCCCB;

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#71528

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID13104.1 )

* These data were automatically processed and therefore are not curated

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Ochrobactrum tritici strain DSM 13340

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