Brucella tritici (DSM 13340, NBRC 102585, CCUG 47104)

Name: Brucella tritici (DSM 13340, NBRC 102585, CCUG 47104)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1884

Type strain

Strain Designation SCII24

Culture col. no. DSM 13340 NBRC 102585 CCUG 47104 LMG 18957 SCII24 1 more

NCBI tax ID(s) 94626

Special Collections DSMZ CCUG Literature strains UCCCB

History <- M. Lebuhn; SCII24 Lebuhn M. DSV-DEVM and GSF - NationalResearch Center forEnvironment and Health

Links

Brucella tritici SCII24 is an aerobe, Gram-negative, motile bacterium that was isolated from rhizosphere of wheat.

Gram-negative motile rod-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 13104

LMG 18957,DSM 13340,CCM 7181,BCRC 17250,CCRC 17250,CCUG 47104,NBRC 102585,CIP 107374

@ref 20215

Last LPSN update 2026-02-09 11:18:12

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Hyphomicrobiales

Family Brucellaceae

Genus Brucella

Species Brucella tritici

Full scientific name Brucella tritici (Lebuhn et al. 2000) Hördt et al. 2020

Synonyms (1)

Ochrobactrum tritici

BacDive ID	Other strains from **Brucella tritici** (1)	Type strain
1885	B. tritici LAIII106, DSM 13341

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Flagellum arrangement	Confidence
23335	negative	0.6-1.4 µm	0.4-0.6 µm	rod-shaped	peritrichous
125439	negative					99.4
125438	negative					99.5

Colony morphology

@ref	Colony color	Medium used
23335	opaque	NA

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
5003	NUTRIENT AGAR (DSMZ Medium 1)	Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
23335	Nutrient agar (NA)
33780	MEDIUM 328- for nutrient agar		Distilled water make up to (1000.000 ml);Agar (15.000 g);Peptone (5.000g);Beef extract (3.000 g)

Culture temp

@ref	Growth	Type	Temperature (°C)
5003	positive	growth	30
23335	positive	growth	4-40
23335	positive	optimum	30
33780	positive	growth	30
57511	positive	growth	30
67772	positive	minimum	4
67772	positive	optimum	30
67772	positive	maximum	40

Culture pH

@ref	Ability	Type	PH	PH range
67772	positive	maximum	9.0
67772	positive	minimum	3.0
23335	positive	optimum	6.0-7.0
67772	positive	optimum	6.0-7.0
23335	positive	growth	3.0-9.0	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
23335	aerobe
57511	aerobe
125439	aerobe	96.4

Spore formation

@ref	Spore formation	Confidence
125439		98.3
125438		90.664

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23335	17521 ChEBI	(-)-quinic acid	-	assimilation
23335	64552 ChEBI	2-hydroxybutyrate	-	assimilation
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
23335	22599 ChEBI	arabinose	-	assimilation
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
23335	17057 ChEBI	cellobiose	-	assimilation
23335	16947 ChEBI	citrate	+/-	assimilation
23335	17784 ChEBI	D-glucosaminic acid	+	assimilation
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
23335	16551 ChEBI	D-trehalose	+	assimilation
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
23335	17126 ChEBI	DL-carnitine	+/-	assimilation
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
23335	28066 ChEBI	gentiobiose	-	assimilation
23335	24265 ChEBI	gluconate	+	assimilation
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
23335	17754 ChEBI	glycerol	-	assimilation
23335	14336 ChEBI	glycerol 1-phosphate	+/-	assimilation
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
23335	15792 ChEBI	malonate	-	assimilation
23335	17306 ChEBI	maltose	+	assimilation
68369	17306 ChEBI	maltose	+	assimilation	from API 20NE
23335	37684 ChEBI	mannose	-	assimilation
23335	28053 ChEBI	melibiose	-	assimilation
68369	59640 ChEBI	N-acetylglucosamine	+	assimilation	from API 20NE
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
23335	15963 ChEBI	ribitol	+	assimilation
23335	41865 ChEBI	sebacic acid	+	assimilation
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	+	hydrolysis	from API 20NE
23335	16704 ChEBI	uridine	+/-	assimilation
23335	17151 ChEBI	xylitol	-	assimilation

Antibiotic resistance

@ref	ChEBI	Metabolite
23335	17698	chloramphenicol
23335	37943	colistin
23335	37943	colistin
23335	8309	polymyxin b

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
23335	catalase	+	1.11.1.6
23335	cytochrome oxidase		1.9.3.1
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
68369	gelatinase	-		from API 20NE
68369	urease	+	3.5.1.5	from API 20NE

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
5003	+	-	-	-	+	-	-	-	+	+	+	+/-	+	+	+	+	-	+	+	-	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host Body-Site	#Plant	#Rhizoplane
#Host	#Plants	#Herbaceous plants (Grass,Crops)

Isolation

@ref	Sample type	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent
5003	rhizosphere of wheat			France	FRA	Europe
23335	rhizoplane of wheat (Triticum aestivum)
57511	Wheat rhizoplane	1997	Grignon	France	FRA	Europe
67772	Wheat rhizoplane		Auvergne-Rhône-Alpes, Grignon	France	FRA	Europe

Taxonmaps

69479

Global distribution of 16S sequence AB681865 (>99% sequence identity) for Ochrobactrum from Microbeatlas

Aquatic Samples	339
Soil Samples	432
Animal Samples	1469
Plant Samples	154
Total Samples	2394

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
5003	1	Risk group (German classification) According to TRBA 466
67772	1

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67772	ASM893229v1 assembly for Brucella tritici LMG 18957	contig	GCA_008932295	94626.9	8110296521	94626	38.67
66792	ASM1239524v1 assembly for Brucella tritici DSM 13340	contig	GCA_012395245	94626.11		94626	4.3

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Ochrobactrum tritici gene for 16S rRNA, partial sequence, strain: NBRC 102585	AB681865	1408	94626
20218	Ochrobactrum tritici 16S rRNA gene (partial), 23S rRNA gene (partial), tRNA-Ile gene, tRNA-Ala gene and ITS1, type strain SCII24, allele B	AJ867329	964	94626
20218	Ochrobactrum tritici 16S rRNA gene (partial), 23S rRNA gene (partial), tRNA-Ile gene, tRNA-Ala gene and ITS1, type strain SCII24, allele A	AJ867330	934	94626
23335	Ochrobactrum tritici partial 16S rRNA gene, strain SCII24	AJ242584	1398	94626

GC content

@ref	GC-content (mol%)	Method
23335	59.00	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Brucella tritici DSM 13340
NCBI: GCA_012395245

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	98.30	no
125439	motility	BacteriaNetⓘ	yes	70.10	no
125439	gram_stain	BacteriaNetⓘ	negative	99.40	no
125439	oxygen_tolerance	BacteriaNetⓘ	aerobe	96.40	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Ochrobactrum tritici strain DSM 13340
PATRIC: 94626.11

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	99.50	yes
125438	anaerobic	anaerobicⓘ	no	93.29	yes
125438	aerobic	aerobicⓘ	yes	83.95	no
125438	spore-forming	spore-formingⓘ	no	90.66	no
125438	thermophilic	thermophileⓘ	no	98.70	no
125438	flagellated	motile2+ⓘ	yes	63.11	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Analysis of 1,000+ Type-Strain Genomes Substantially Improves Taxonomic Classification of Alphaproteobacteria.	Hordt A, Lopez MG, Meier-Kolthoff JP, Schleuning M, Weinhold LM, Tindall BJ, Gronow S, Kyrpides NC, Woyke T, Goker M.	Front Microbiol	10.3389/fmicb.2020.00468	2020
Metabolism	Arsenic accumulation by a rhizosphere bacterial strain Ochrobactrum tritici reduces rice plant arsenic levels.	Moens M, Branco R, Morais PV	World J Microbiol Biotechnol	10.1007/s11274-020-2800-0	2020
Metabolism	Immobilization of Ochrobactrum tritici As5 on PTFE thin films for arsenite biofiltration.	Branco R, Sousa T, Piedade AP, Morais PV	Chemosphere	10.1016/j.chemosphere.2015.12.025	2015
Metabolism	Hyper Accumulation of Arsenic in Mutants of Ochrobactrum tritici Silenced for Arsenite Efflux Pumps.	Sousa T, Branco R, Piedade AP, Morais PV	PLoS One	10.1371/journal.pone.0131317	2015
Metabolism	Identification of an aox system that requires cytochrome c in the highly arsenic-resistant bacterium Ochrobactrum tritici SCII24.	Branco R, Francisco R, Chung AP, Morais PV	Appl Environ Microbiol	10.1128/AEM.02798-08	2009
Genetics	Sequencing and expression of two arsenic resistance operons with different functions in the highly arsenic-resistant strain Ochrobactrum tritici SCII24T.	Branco R, Chung AP, Morais PV	BMC Microbiol	10.1186/1471-2180-8-95	2008
Phylogeny	Ochrobactrum cytisi sp. nov., isolated from nodules of Cytisus scoparius in Spain.	Zurdo-Pineiro JL, Rivas R, Trujillo ME, Vizcaino N, Carrasco JA, Chamber M, Palomares A, Mateos PF, Martinez-Molina E, Velazquez E	Int J Syst Evol Microbiol	10.1099/ijs.0.64613-0	2007
Phylogeny	Taxonomic characterization of Ochrobactrum sp. isolates from soil samples and wheat roots, and description of Ochrobactrum tritici sp. nov. and Ochrobactrum grignonense sp. nov.	Lebuhn M, Achouak W, Schloter M, Berge O, Meier H, Barakat M, Hartmann A, Heulin T	Int J Syst Evol Microbiol	10.1099/00207713-50-6-2207	2000

References

#5003	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13340
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#23335	M Lebuhn, W Achouak, M Schloter, O Berge, H Meier, M Barakat, A Hartmann, T Heulin: Taxonomic characterization of Ochrobactrum sp. isolates from soil samples and wheat roots, and description of Ochrobactrum tritici sp. nov. and Ochrobactrum grignonense sp. nov.. IJSEM 50: 2207 - 2223 2000 ( DOI 10.1099/00207713-50-6-2207 , PubMed 11155998 )
#33780	; Curators of the CIP;
#57511	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 47104
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67772	The University of Coimbra Bacteria Culture Collection (UCCCB) ; Curators of the UCCCB;
#68369	Automatically annotated from API 20NE .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Brucella tritici (DSM 13340, NBRC 102585, CCUG 47104)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_008932295

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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