Brucella grignonensis (DSM 13338, NBRC 102586, CCUG 46362)

Name: Brucella grignonensis (DSM 13338, NBRC 102586, CCUG 46362)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1882

Type strain

Strain Designation OgA9a

Culture col. no. DSM 13338 NBRC 102586 CCUG 46362 CIP 107373 LMG 18954 1 more

NCBI tax ID(s) 94627

Special Collections DSMZ CCUG CIP Literature strains

History <- M. Lebuhn; OgA9a CIP <- 2002, A. Hartmann, Helmholz Center, Munich-Neuherberg, Germany: strain OgA9a <- M. Lebuhn

Links

Brucella grignonensis OgA9a is an obligate aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from soil.

Gram-negative motile rod-shaped colony-forming obligate aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 13105

LMG 18954,DSM 13338,CCUG 46362,CIP 107373,CCM 7180,BCRC 17249,CCRC 17249,NBRC 102586

@ref 20215

Last LPSN update 2026-02-09 11:18:12

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Hyphomicrobiales

Family Brucellaceae

Genus Brucella

Species Brucella grignonensis

Full scientific name Brucella grignonensis (Lebuhn et al. 2000) Hördt et al. 2020

Synonyms (1)

Ochrobactrum grignonense

BacDive ID	Other strains from **Brucella grignonensis** (1)	Type strain
1883	B. grignonensis OgA9c, DSM 13339, LMG 18955

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Flagellum arrangement	Confidence
23335	negative	0.6-1.2 µm	0.4 µm	rod-shaped	peritrichous
119740	negative			rod-shaped
125438	negative					97.5
125439	negative					99.6

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period	Medium used	Hemolysis ability
23335	1 mm	milky opaque	circular	1 day	NA
119740						1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
5001	NUTRIENT AGAR (DSMZ Medium 1)	Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
23335	Nutrient agar (NA)
33779	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
119740	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
5001	positive	growth	30
23335	positive	growth	4-40
23335	positive	optimum	30
33779	positive	growth	30
119740	positive	growth	15-37
119740	negative	growth	5
119740	negative	growth	41
119740	negative	growth	45

Culture pH

@ref	Ability	Type	PH	PH range
23335	positive	optimum	6.0-7.0
23335	positive	growth	3.0-9.0	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
119740	obligate aerobe
125439	aerobe	96.4

Spore formation

@ref	Spore formation	Confidence
125439		98.8

Halophily

@ref	Salt	Growth	Tested relation	Concentration
119740	NaCl	positive	growth	0-4 %
119740	NaCl		growth	6 %
119740	NaCl		growth	8 %
119740	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23335	17521 ChEBI	(-)-quinic acid	-	assimilation
23335	64552 ChEBI	2-hydroxybutyrate	+	assimilation
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
23335	22599 ChEBI	arabinose	+	builds acid from
23335	22599 ChEBI	arabinose	+/-	assimilation
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
23335	17057 ChEBI	cellobiose	+/-	assimilation
23335	16947 ChEBI	citrate	+	assimilation
119740	16947 ChEBI	citrate	+	carbon source
23335	17784 ChEBI	D-glucosaminic acid	-	assimilation
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68377	17634 ChEBI	D-glucose	+	builds acid from	from API NH
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
23335	16551 ChEBI	D-trehalose	-	assimilation
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
23335	17126 ChEBI	DL-carnitine	+/-	assimilation
119740	4853 ChEBI	esculin	-	hydrolysis
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
23335	28066 ChEBI	gentiobiose	+/-	assimilation
23335	24265 ChEBI	gluconate	+/-	assimilation
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
23335	17754 ChEBI	glycerol	+	assimilation
23335	14336 ChEBI	glycerol 1-phosphate	+/-	assimilation
119740	606565 ChEBI	hippurate	+	hydrolysis
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
23335	15792 ChEBI	malonate	+	assimilation
119740	15792 ChEBI	malonate	+	assimilation
23335	17306 ChEBI	maltose	-	assimilation
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
68377	17306 ChEBI	maltose	-	builds acid from	from API NH
23335	37684 ChEBI	mannose	+	assimilation
23335	28053 ChEBI	melibiose	+	assimilation
23335	28053 ChEBI	melibiose	+	builds acid from
68369	59640 ChEBI	N-acetylglucosamine	+	assimilation	from API 20NE
119740	17632 ChEBI	nitrate	+	reduction
119740	17632 ChEBI	nitrate	+	respiration
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
119740	16301 ChEBI	nitrite	+	reduction
23335	15963 ChEBI	ribitol	-	assimilation
23335	41865 ChEBI	sebacic acid	-	assimilation
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE
23335	16704 ChEBI	uridine	+	assimilation
23335	17151 ChEBI	xylitol	+/-	assimilation

Antibiotic resistance

@ref	Metabolite	ChEBI
119740	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
23335	chloramphenicol	17698
23335	colistin	37943
23335	polymyxin b	8309

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE
119740	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test	Voges-proskauer-test	Methylred-test
119740	15688 ChEBI	acetoin		-
119740	17234 ChEBI	glucose			-
68369	35581 ChEBI	indole	-			from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
119740	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68377	alkaline phosphatase	-	3.1.3.1	from API NH
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
119740	amylase	-
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68382	beta-galactosidase	-	3.2.1.23	from API zym
119740	beta-galactosidase	-	3.2.1.23
68377	beta-galactosidase	-	3.2.1.23	from API NH
68382	beta-glucosidase	-	3.2.1.21	from API zym
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68377	beta-lactamase	+	3.5.2.6	from API NH
119740	caseinase	-	3.4.21.50
119740	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
119740	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	-		from API zym
119740	gamma-glutamyltransferase	+	2.3.2.2
119740	gelatinase	-
68369	gelatinase	-		from API 20NE
119740	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
119740	lipase	-
68382	lipase (C 14)	-		from API zym
119740	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
119740	ornithine decarboxylase	-	4.1.1.17
119740	oxidase	+
119740	phenylalanine ammonia-lyase	-	4.3.1.24
68382	trypsin	+	3.4.21.4	from API zym
119740	tryptophan deaminase	-
119740	tween esterase	-
23335	urease	-	3.5.1.5
119740	urease	+	3.5.1.5
68369	urease	-	3.5.1.5	from API 20NE
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
119740	-	+	+	-	-	+	-	-	+	-	+	-	-	-	-	-	-	-	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
5001	+	-	-	-	-	-	-	-	+	+	+	+/-	+	-	+	+	-	+	+	-	not determinedn.d.
5001	+	-	-	-	-	-	-	-	+	+	+	-	+	-	+	+	-	+	+	-	+

API NH

@ref	PEN	GLU	FRU	MAL	SAC	ODC	URE	LIP	PAL	beta GAL	ProA	GGT	IND
5001	+	+	-	-	+	+/-	+	-	-	-	+	-	+
5001	+	+	+	-	-	+	+/-	+	-	-	-	+	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
119740	not determinedn.d.	+	+	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	+	+	-	+	-	-	+	+	+	+	-	-	-	-	-	-	+	+	-	-	-	-	+	-	-	+	+	+	-	-	+	+	+	+	-	-	+	+	-	+	-	-	+	-	-	-	-	-	+	-	+	-	+	-	-	-	+	+	+	+	-	-	-	+	-	+	+	+	+	-	-	+	+	+	-	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
5001	soil		France	FRA	Europe
23335	soil of the Grignon Experimental Station
57167	Soil	Grignon	France	FRA	Europe
119740	Environment, Soil samples and wheat roots	Grignon	France	FRA	Europe	1998

Taxonmaps

69479

Global distribution of 16S sequence AM490619 (>99% sequence identity) for Ochrobactrum from Microbeatlas

Aquatic Samples	2
Soil Samples	8
Animal Samples	21
Plant Samples	2
Total Samples	33

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
5001	1	Risk group (German classification) According to TRBA 466
119740	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM4193008v1 assembly for Brucella grignonensis LMG 18954	scaffold	GCA_041930085			94627	34.43
66792	ASM225250v1 assembly for Brucella grignonensis OgA9a	contig	GCA_002252505	94627.10	2834567989	94627	34.3
66792	O.grignonense_LMG_18954.fasta assembly for Brucella grignonensis LMG 18954	contig	GCA_012103085	94627.15		94627	5.21

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Ochrobactrum grignonense gene for 16S rRNA, partial sequence, strain: NBRC 102586	AB681866	1407	94627
20218	Ochrobactrum grignonense partial 16S rRNA gene, strain Oga9a	AM490619	1387	94627
23335	Ochrobactrum grignonense 16S rRNA gene (partial), 23S rRNA gene (partial), tRNA-Ile gene, tRNA-Ala gene and ITS1, type strain OgA9a	AJ242581	2349	94627

GC content

@ref	GC-content (mol%)	Method
23335	58.00	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Brucella grignonensis OgA9a
NCBI: GCA_002252505

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	98.80	no
125439	motility	BacteriaNetⓘ	yes	77.70	no
125439	gram_stain	BacteriaNetⓘ	negative	99.60	no
125439	oxygen_tolerance	BacteriaNetⓘ	aerobe	96.40	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Ochrobactrum grignonense strain LMG 18954
PATRIC: 94627.15

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	97.50	no
125438	anaerobic	anaerobicⓘ	no	94.87	no
125438	aerobic	aerobicⓘ	yes	83.88	yes
125438	spore-forming	spore-formingⓘ	no	87.24	no
125438	thermophilic	thermophileⓘ	no	97.44	yes
125438	flagellated	motile2+ⓘ	yes	52.46	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Analysis of 1,000+ Type-Strain Genomes Substantially Improves Taxonomic Classification of Alphaproteobacteria.	Hordt A, Lopez MG, Meier-Kolthoff JP, Schleuning M, Weinhold LM, Tindall BJ, Gronow S, Kyrpides NC, Woyke T, Goker M.	Front Microbiol	10.3389/fmicb.2020.00468	2020
Phylogeny	Taxonomic characterization of Ochrobactrum sp. isolates from soil samples and wheat roots, and description of Ochrobactrum tritici sp. nov. and Ochrobactrum grignonense sp. nov.	Lebuhn M, Achouak W, Schloter M, Berge O, Meier H, Barakat M, Hartmann A, Heulin T	Int J Syst Evol Microbiol	10.1099/00207713-50-6-2207	2000

References

#5001	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13338
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#23335	M Lebuhn, W Achouak, M Schloter, O Berge, H Meier, M Barakat, A Hartmann, T Heulin: Taxonomic characterization of Ochrobactrum sp. isolates from soil samples and wheat roots, and description of Ochrobactrum tritici sp. nov. and Ochrobactrum grignonense sp. nov.. IJSEM 50: 2207 - 2223 2000 ( DOI 10.1099/00207713-50-6-2207 , PubMed 11155998 )
#33779	; Curators of the CIP;
#57167	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 46362
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68369	Automatically annotated from API 20NE .
#68377	Automatically annotated from API NH .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#119740	Collection of Institut Pasteur ; Curators of the CIP; CIP 107373
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Brucella grignonensis (DSM 13338, NBRC 102586, CCUG 46362)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API zym

API 20NE

API NH

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_041930085

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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