Name: Bacillus halotolerans WS 1087
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 1847

Type strain:

Species: Bacillus halotolerans

Strain Designation: WS 1087, 6721

Culture col. no.: CCUG 47676, CIP 67.21, DSM 8802, ATCC 25096, JCM 12400, BCRC 14861

Strain history: CIP <- 1967, B. Delaporte, Inst. Pasteur, Paris, France

NCBI tax ID(s): 260554 (species)

SI-ID 389308

ATCC 25096, DSM 8802, CIP 67.21, CCUG 47676, JCM 12400, BCRC 14861, CCRC 14861

Special Collections

Other strains from Bacillus halotolerans

Section

When using BacDive for research please cite our paper

Bacillus halotolerans WS 1087 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from soil.

Gram-positive
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus halotolerans**
	Full Scientific Name (LPSN)	Bacillus halotolerans (Delaporte and Sasson 1967) Tindall 2017

	Synonym	Bacillus axarquiensis
	Synonym	Bacillus malacitensis
	Synonym	Brevibacterium halotolerans
	Synonym	"Bacillus* halotolerans"*

Information on morphological and physiological properties Morphology

[Ref.: #119823]	Gram stain	positive

[Ref.: #119823]	Cell shape	rod-shaped

[Ref.: #119823]	Motility	yes

[Ref.: #20210]	Colony color	Ivory (1014)
[Ref.: #20210]	Incubation period	10-14 days
[Ref.: #20210]	Cultivation medium used	ISP 2

[Ref.: #20210]	Colony color	Light ivory (1015)
[Ref.: #20210]	Incubation period	10-14 days
[Ref.: #20210]	Cultivation medium used	ISP 3

[Ref.: #20210]	Colony color	Rapeseed yellow (1021)
[Ref.: #20210]	Incubation period	10-14 days
[Ref.: #20210]	Cultivation medium used	ISP 4

[Ref.: #20210]	Colony color	Traffic yellow (1023)
[Ref.: #20210]	Incubation period	10-14 days
[Ref.: #20210]	Cultivation medium used	ISP 6

[Ref.: #20210]	Multicellular complex forming ability	no
[Ref.: #20210]	Medium Name (multicellularity)	ISP 2

[Ref.: #20210]	Multicellular complex forming ability	no
[Ref.: #20210]	Medium Name (multicellularity)	ISP 3

[Ref.: #20210]	Multicellular complex forming ability	no
[Ref.: #20210]	Medium Name (multicellularity)	ISP 4

[Ref.: #20210]	Multicellular complex forming ability	no
[Ref.: #20210]	Medium Name (multicellularity)	ISP 6

Information on culture and growth conditions Culture and growth conditions

[Ref.: #20210]

Culture medium

ISP 2

[Ref.: #20210]

Culture medium growth

[Ref.: #20210]

Culture medium composition

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Culture medium

ISP 3

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 4

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 6

[Ref.: #20210]

Culture medium growth

[Ref.: #20210]

Culture medium composition

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[Ref.: #42212]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #42212]

Culture medium growth

[Ref.: #42212]

Culture medium composition

expand / minimize

[Ref.: #3408]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #3408]

Culture medium growth

[Ref.: #3408]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3408]

Culture medium composition

expand / minimize

[Ref.: #119823]

Culture medium

CIP Medium 72

[Ref.: #119823]

Culture medium growth

[Ref.: #119823]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #119823]

Culture medium

CIP Medium 3

[Ref.: #119823]

Culture medium growth

[Ref.: #119823]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #3408]	growth	30 °C
[Ref.: #20210]	optimum	28 °C
[Ref.: #42212]	growth	30 °C
[Ref.: #57815]	growth	30 °C
[Ref.: #67770]	growth	28 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #57815]	Oxygen tolerance	aerobe
[Ref.: #119823]	Oxygen tolerance	obligate aerobe

[Ref.: #125438]

Ability of spore formation

yes Confidence in %94.4

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #20210]		NaCl		maximum	2.5	%

[Ref.: #3408]	Murein short key	A31
[Ref.: #3408]	Murein types	A1gamma m-Dpm-direct

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #20210]	arabinose ChEBI	-
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #20210]	cellulose ChEBI	-
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #20210]	fructose ChEBI	-
[Ref.: #68379]	gelatin ChEBI	+	hydrolysis	* from API Coryne, API 20E
[Ref.: #20210]	glucose ChEBI	-
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68379]	maltose ChEBI	+	fermentation	* from API Coryne
[Ref.: #20210]	mannitol ChEBI	-
[Ref.: #20210]	myo-inositol ChEBI	-
[Ref.: #119823]	nitrate ChEBI	+	reduction
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #119823]	nitrite ChEBI	-	reduction
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #20210]	raffinose ChEBI	-
[Ref.: #20210]	rhamnose ChEBI	-
[Ref.: #20210]	sucrose ChEBI	-
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68379]	urea ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
[Ref.: #20210]	xylose ChEBI	-
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #119823]	indole ChEBI	no
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68379]	alpha-glucosidase	-	3.2.1.20	* from API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68368]	beta-galactosidase	+	3.2.1.23	* from API 20E
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API Coryne
[Ref.: #119823]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68379]	gelatinase	+		* from API Coryne, API 20E
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym, API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #119823]	oxidase	-
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #119823]	urease	-	3.5.1.5
[Ref.: #68379]	urease	+	3.5.1.5	* from API Coryne
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #20210]	646	+	-	-	-	-	-	-	-	-	+	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #20210]	477	-	-	+	-	-	-	-	-	-	+	+	not determinedn.d.	-	-	-	+	+	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #20210]	988	not determinedn.d.	-	-	+	-	-	-	-	-	-	+	+	-	-	-	+	+	-	-	-
[Ref.: #119823]	13123	-	+	+	-	-	-	-	-	-	-	+	+	-	+	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3408]	Sample type/isolated from	soil
[Ref.: #3408]	Country	Morocco
[Ref.: #3408]	Country ISO 3 Code	MAR
[Ref.: #3408]	Continent	Africa

[Ref.: #57815]	Sample type/isolated from	Soil
[Ref.: #57815]	Country	Morocco
[Ref.: #57815]	Country ISO 3 Code	MAR
[Ref.: #57815]	Continent	Africa

[Ref.: #67770]	Sample type/isolated from	Arid soil
[Ref.: #67770]	Country	Morocco
[Ref.: #67770]	Country ISO 3 Code	MAR
[Ref.: #67770]	Continent	Africa

[Ref.: #119823]	Sample type/isolated from	Environment, Soil
[Ref.: #119823]	Country	Morocco
[Ref.: #119823]	Country ISO 3 Code	MAR
[Ref.: #119823]	Continent	Africa
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AM747812 (>99% sequence identity) for Bacillus from Microbeatlas

Aquatic Samples	2854
Soil Samples	3768
Animal Samples	9674
Plant Samples	1905
Total Samples	18201

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #20210]	Biosafety level	1
[Ref.: #3408]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119823]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Brevibacterium halotolerans partial 16S rRNA gene, type strain LMG 21660T	AJ620368	1513	GenBank ENA	260554 tax ID
[Ref.: #124043]	Bacillus halotolerans strain DSM 8802 16S ribosomal RNA gene, partial sequence.	ON081966	772	GenBank ENA	260554 tax ID	*
[Ref.: #124043]	Bacillus halotolerans strain ATCC 25096 16S ribosomal RNA gene, partial sequence.	OQ876681	1449	GenBank ENA	260554 tax ID	*
[Ref.: #124043]	Bacillus halotolerans strain DSM 8802 16S ribosomal RNA gene, partial sequence.	MN526998	498	GenBank ENA	260554 tax ID	*
[Ref.: #20218]	[Brevibacterium] halotolerans 16S rRNA gene, strain DSM 8802	AM747812	1545	GenBank ENA	260554 tax ID	*
[Ref.: #124043]	Bacillus halotolerans strain DSM 8802 16S ribosomal RNA gene, partial sequence.	MN527277	498	GenBank ENA	260554 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Bacillus halotolerans ATCC 25096	GCA_001517105	contig	GenBank ENA	260554 tax ID
[Ref.: #66792]	[Brevibacterium] halotolerans strain ATCC 25096	260554.3	wgs	PATRIC	260554 tax ID	*
[Ref.: #66792]	Bacillus halotolerans ATCC 25096	2716884207	draft	JGI IMG/M	260554 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %	In training data
	Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: [Brevibacterium] halotolerans strain ATCC 25096 PATRIC: 260554.3
[Ref.: #125438]	gram-positive	gram-positiveⓘ	yes	82.539	no
[Ref.: #125438]	anaerobic	anaerobicⓘ	no	88.96	no
[Ref.: #125438]	spore-forming	spore-formingⓘ	yes	94.4	no
[Ref.: #125438]	aerobic	aerobicⓘ	yes	72.795	no
[Ref.: #125438]	thermophile	thermophilicⓘ	no	94.986	no
[Ref.: #125438]	motile2+	flagellatedⓘ	yes	88.277	no

	Model	Trait	Prediction	Confidence in %
	Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Bacillus halotolerans ATCC 25096 NCBI: GCA_001517105
[Ref.: #125439]	BacteriaNet	spore_formationⓘ	yes	80.8
[Ref.: #125439]	BacteriaNet	motilityⓘ	yes	75.6
[Ref.: #125439]	BacteriaNet	gram_stainⓘ	variable	86.7
[Ref.: #125439]	BacteriaNet	oxygen_toleranceⓘ	obligate aerobe	82.4

Availability in culture collections External links

[Ref.: #3408]

Culture collection no.

CCUG 47676, CIP 67.21, DSM 8802, ATCC 25096, JCM 12400, BCRC 14861

[Ref.: #71492]

SI-ID 389308

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Polyhydroxyalkanoate synthesis by bacteria isolated from landfill and ETP with pomegranate peels as carbon source.	Rayasam V, Chavan P, Kumar T	Arch Microbiol	10.1007/s00203-020-01995-9	2020	*
Phylogeny	Reclassification of Brevibacterium halotolerans DSM8802 as Bacillus halotolerans comb. nov. Based on Microbial and Biochemical Characterization and Multiple Gene Sequence.	Ben-Gad D, Gerchman Y	Curr Microbiol	10.1007/s00284-016-1143-4	2016	*

References

#3408

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 8802

#20210

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#42212 ; Curators of the CIP;
#57815 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 47676

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#71492

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID389308.1 )

#119823 Collection of Institut Pasteur ; Curators of the CIP; CIP 67.21

#124043

Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

#125438

Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )

#125439

Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

* These data were automatically processed and therefore are not curated

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[Brevibacterium] halotolerans strain ATCC 25096

Bacillus halotolerans ATCC 25096

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