Name: Brevibacterium epidermidis DSM 20660
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1838

Type strain:

Species: Brevibacterium epidermidis

Culture col. no.: DSM 20660, ATCC 35514, NCDO 2286, CCUG 30540, NCTC 11083, JCM 2593, BCRC 12215, CCM 4098, CECT 4162, CGMCC 1.1929, CIP 102110, IFO 14811, IMET 10998, KCTC 3090, LMG 21455, NBRC 14811, NCIMB 702286, VKM Ac-2108, VTT E-032416

Strain history: NCDO 2286 <-- D. G. Pitcher P159.

NCBI tax ID(s): 1349751 (strain), 1698 (species)

Other strains from Brevibacterium epidermidis

B. epidermidis 615, DSM 9586
B. epidermidis DSM 20659, NCDO 2285, JCM 2592, ...
B. epidermidis CCUG 32343

Section

Brevibacterium epidermidis DSM 20660 is a mesophilic bacterium that was isolated from human skin.

mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Brevibacteriaceae*
	Genus	*Brevibacterium*
	Species	**Brevibacterium epidermidis**
	Full Scientific Name (PNU)	Brevibacterium epidermidis Collins et al. 1983

Information on morphological and physiological properties Morphology

[Ref.: #18768]	Colony color	Oyster white
[Ref.: #18768]	Incubation period	10-14 days
[Ref.: #18768]	Cultivation medium used	ISP 2

[Ref.: #18768]	Colony color	Oyster white
[Ref.: #18768]	Incubation period	10-14 days
[Ref.: #18768]	Cultivation medium used	ISP 3

[Ref.: #18768]	Colony color	Oyster white
[Ref.: #18768]	Incubation period	10-14 days
[Ref.: #18768]	Cultivation medium used	ISP 4

[Ref.: #18768]	Colony color	Oyster white
[Ref.: #18768]	Incubation period	10-14 days
[Ref.: #18768]	Cultivation medium used	ISP 5

[Ref.: #18768]	Colony color	Ivory
[Ref.: #18768]	Incubation period	10-14 days
[Ref.: #18768]	Cultivation medium used	ISP 6

[Ref.: #18768]	Multicellular complex forming ability	no
[Ref.: #18768]	Medium Name (multicellularity)	ISP 2

[Ref.: #18768]	Multicellular complex forming ability	no
[Ref.: #18768]	Medium Name (multicellularity)	ISP 3

[Ref.: #18768]	Multicellular complex forming ability	no
[Ref.: #18768]	Medium Name (multicellularity)	ISP 4

[Ref.: #18768]	Multicellular complex forming ability	no
[Ref.: #18768]	Medium Name (multicellularity)	ISP 5

[Ref.: #18768]	Multicellular complex forming ability	no
[Ref.: #18768]	Medium Name (multicellularity)	ISP 6

Information on culture and growth conditions Culture and growth conditions

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Culture medium

ISP 2

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 3

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 4

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 5

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 6

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Culture medium growth

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Culture medium composition

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Culture medium

CORYNEBACTERIUM AGAR (DSMZ Medium 53)

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

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Culture medium composition

expand / minimize

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Culture medium

MEDIUM 3 - Columbia agar

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Culture medium growth

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Culture medium composition

expand / minimize

		Temperature
[Ref.: #8880]	growth	30 °C
[Ref.: #18768]	optimum	28 °C
[Ref.: #40309]	growth	30 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #8880]	Temperature range	mesophilic
[Ref.: #18768]	Temperature range	mesophilic
[Ref.: #40309]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #18768]		NaCl		maximum	7.5	%

[Ref.: #8880]	Murein short key	A31
[Ref.: #8880]	Murein types	A1gamma m-Dpm-direct

[Ref.: #67770]

Observation

quinones: MK-8(H₂)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #18768]	arabinose ChEBI	+
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #18768]	cellulose ChEBI	-
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #18768]	fructose ChEBI	-
[Ref.: #68379]	gelatin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #18768]	glucose ChEBI	+
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #18768]	mannitol ChEBI	-
[Ref.: #18768]	myo-inositol ChEBI	+
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #18768]	raffinose ChEBI	+
[Ref.: #18768]	rhamnose ChEBI	-
[Ref.: #18768]	sucrose ChEBI	+
[Ref.: #68379]	sucrose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68379]	urea ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
[Ref.: #18768]	xylose ChEBI	+
	Only first 10 entries are displayed. Click here to see all.

	Metabolite
[Ref.: #68368]	acetoin ChEBI	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	* from API 20E
[Ref.: #68368]	indole ChEBI	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym, API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym, API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68379]	beta-galactosidase	+	3.2.1.23	* from API Coryne
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym, API 20E
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym, API Coryne
[Ref.: #68379]	beta-glucuronidase	+	3.2.1.31	* from API Coryne
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	+		* from API Coryne
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	+		* from API zym
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym, API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #68379]	pyrazinamidase	+	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68379]	urease	+	3.5.1.5	* from API Coryne
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #50138]

Fatty acid	Percentage	ECL
C15:0 ANTEISO	59.6	14.711
C17:0 anteiso	24.6	16.722
C15:0 ISO	8.5	14.621
C16:0 iso	3.3	15.626
C17:1 ω9c ANTEISO	2.3	16.525
C17:0 iso	1.1	16.629
C16:0	0.5	16

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #18768]	244	-	-	-	-	+	-	-	-	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #18768]	240	-	+	+	+	+	+	-	-	-	+	+	not determinedn.d.	-	-	-	-	-	-	+	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #18768]	333	not determinedn.d.	+	+	+	+	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8880]	Sample type/isolated from	human skin

[Ref.: #50138]	Sample type/isolated from	Skin

[Ref.: #67770]	Sample type/isolated from	Human skin
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body-Site	#Organ	#Skin, Nail, Hair

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence X76565 (>99% sequence identity) for Brevibacterium from Microbeatlas

Aquatic Samples	205
Soil Samples	327
Animal Samples	1757
Plant Samples	42
Total Samples	2331

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8880]	Pathogenicity (human)	yes, in single cases
[Ref.: #8880]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #18768]	Biosafety level	1+	Hazard group

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	B.epidermidis (NCDO 2286) gene for 16S ribosomal RNA	X76565	1482	ENA	1698 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Brevibacterium epidermidis NBRC 14811 NBRC 14811	GCA_001570805	contig	GenBank	1349751 tax ID
[Ref.: #66792]	Brevibacterium epidermidis NBRC 14811	1349751.3	wgs	PATRIC	1349751 tax ID	*
[Ref.: #66792]	Brevibacterium epidermidis NBRC 14811	2731957605	draft	JGI IMG/M	1349751 tax ID	*

[Ref.: #8880]	GC-content	63.5 mol%
[Ref.: #67770]	GC-content	64.3 mol%	genome sequence analysis

Availability in culture collections External links

[Ref.: #8880]

Culture collection no.

DSM 20660, ATCC 35514, NCDO 2286, CCUG 30540, NCTC 11083, JCM 2593, BCRC 12215, CCM 4098, CECT 4162, CGMCC 1.1929, CIP 102110, IFO 14811, IMET 10998, KCTC 3090, LMG 21455, NBRC 14811, NCIMB 702286, VKM Ac-2108, VTT E-032416

[Ref.: #20218]

Associated Passport(s) in StrainInfo

129371, 124599, 124600

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Brevibacterium renqingii sp. nov., isolated from the Daqu of Baijiu.	Yan Y, Xing X, Sun Z, Li J, Hao S, Xu J	Arch Microbiol	10.1007/s00203-021-02212-x	2021	*
Phylogeny	Brevibacterium hankyongi sp. nov., isolated from compost.	Choi KD, Siddiqi MZ, Liu Q, Muhammad Shafi S, Durrani Y, Lee SY, Kang MS, Im WT	Int J Syst Evol Microbiol	10.1099/ijsem.0.002886	2018	*
Phylogeny	Brevibacterium sediminis sp. nov., isolated from deep-sea sediments from the Carlsberg and Southwest Indian Ridges.	Chen P, Zhang L, Wang J, Ruan J, Han X, Huang Y	Int J Syst Evol Microbiol	10.1099/ijsem.0.001506	2016	*
Phylogeny	Brevibacterium siliguriense sp. nov., a facultatively oligotrophic bacterium isolated from river water.	Kumar A, Ince IA, Kati A, Chakraborty R	Int J Syst Evol Microbiol	10.1099/ijs.0.038281-0	2012	*
Phylogeny	Chemotaxonomy and molecular taxonomy of some coryneform clinical isolates.	Carlotti A, Meugnier H, Pommier MT, Villard J, Freney J	Zentralbl Bakteriol	10.1016/s0934-8840(11)80276-8	1993	*
Phylogeny	Brevibacterium casei sp. nov. and Brevibacterium epidermidis sp. nov.	Collins MD, Farrow JA, Goodfellow M, Minnikin DE	Syst Appl Microbiol	10.1016/S0723-2020(83)80023-X	1983	*

References

#8880

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20660

#18768

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40309 ; Curators of the CIP;
#50138 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 30540

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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