Name: Brevibacterium casei CMD1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1834

Type strain:

Species: Brevibacterium casei

Strain Designation: CMD1

Culture col. no.: DSM 20657, ATCC 35513, NCDO 2048, CCUG 29001, JCM 2594, BCRC 12214, CCM 4100, CECT 4161, CGMCC 1.1930, CIP 102111, IFO 14812, IMET 10997, KCTC 3082, KCTC 3085, NBRC 14812, NCIMB 702048, VKM Ac-2114

Strain history: NCDO 2048 <-- M. E. Sharpe CMD1.

NCBI tax ID(s): 1255625 (strain), 33889 (species)

Other strains from Brevibacterium casei

B. casei 108, DSM 9657
B. casei 393, DSM 9658
B. casei 585, DSM 9659
B. casei R6, DSM 20658, NCDO 2051, JCM 2597, ...
B. casei JV801, DSM 29377
B. casei STI09086(IMET), 1136-001,
B. casei STI09087(IMET), 1136-009,
B. casei CIP 104165
B. casei CIP 102489
B. casei CIP 102233
B. casei CIP 102347
B. casei CCUG 27947
B. casei CCUG 30494
B. casei CCUG 31323
B. casei CCUG 31402
B. casei CCUG 31467
B. casei CCUG 32028
B. casei CCUG 32316
B. casei CCUG 32476
B. casei CCUG 32538
B. casei CCUG 33193
B. casei CCUG 33345
B. casei CCUG 34468
B. casei CCUG 34955
B. casei CCUG 35377
B. casei CCUG 35578
B. casei CCUG 37266
B. casei CCUG 43490, CIP 104166, ATCC 51396
B. casei CCUG 44572
B. casei CCUG 46895
B. casei CCUG 51271
B. casei CCUG 51543
B. casei CCUG 53344
B. casei CCUG 53784
B. casei CCUG 53939
B. casei CCUG 55282
B. casei S 53/2 II white, DSM 110887
B. casei 105895, DSM 105895
B. casei JCM 2595, IFO 15228, KCTC 3086, ...
B. casei JCM 2596, CCM 4101, KCTC 3087, ...
B. casei TURIN101221_C9a1, DSM 113548

Section

Brevibacterium casei CMD1 is an aerobe, mesophilic bacterium that produces methane and was isolated from cheddar cheese.

methane production
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Brevibacteriaceae*
	Genus	*Brevibacterium*
	Species	**Brevibacterium casei**
	Full Scientific Name (PNU)	Brevibacterium casei Collins et al. 1983

Information on morphological and physiological properties Morphology

[Ref.: #18455]	Colony color	Light ivory (1012)
[Ref.: #18455]	Incubation period	10-14 days
[Ref.: #18455]	Cultivation medium used	ISP 2

[Ref.: #18455]	Colony color	Light ivory (1015)
[Ref.: #18455]	Incubation period	10-14 days
[Ref.: #18455]	Cultivation medium used	ISP 3

[Ref.: #18455]	Colony color	Colorless
[Ref.: #18455]	Incubation period	10-14 days
[Ref.: #18455]	Cultivation medium used	ISP 5

[Ref.: #18455]	Colony color	Colorless
[Ref.: #18455]	Incubation period	10-14 days
[Ref.: #18455]	Cultivation medium used	ISP 6

[Ref.: #18455]	Colony color	Light ivory (1015)
[Ref.: #18455]	Incubation period	10-14 days
[Ref.: #18455]	Cultivation medium used	ISP 7

[Ref.: #18455]	Multicellular complex forming ability	no
[Ref.: #18455]	Medium Name (multicellularity)	ISP 2

[Ref.: #18455]	Multicellular complex forming ability	no
[Ref.: #18455]	Medium Name (multicellularity)	ISP 3

[Ref.: #18455]	Multicellular complex forming ability	no
[Ref.: #18455]	Medium Name (multicellularity)	ISP 5

[Ref.: #18455]	Multicellular complex forming ability	no
[Ref.: #18455]	Medium Name (multicellularity)	ISP 6

[Ref.: #18455]	Multicellular complex forming ability	no
[Ref.: #18455]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #18455]

Culture medium

ISP 2

[Ref.: #18455]

Culture medium growth

[Ref.: #18455]

Culture medium composition

expand / minimize

[Ref.: #18455]

Culture medium

ISP 7

[Ref.: #18455]

Culture medium growth

[Ref.: #18455]

Culture medium composition

expand / minimize

[Ref.: #18455]

Culture medium

ISP 6

[Ref.: #18455]

Culture medium growth

[Ref.: #18455]

Culture medium composition

expand / minimize

[Ref.: #18455]

Culture medium

ISP 5

[Ref.: #18455]

Culture medium growth

[Ref.: #18455]

Culture medium composition

expand / minimize

[Ref.: #18455]

Culture medium

ISP 3

[Ref.: #18455]

Culture medium growth

[Ref.: #18455]

Culture medium composition

expand / minimize

[Ref.: #8877]

Culture medium

CORYNEBACTERIUM AGAR (DSMZ Medium 53)

[Ref.: #8877]

Culture medium growth

[Ref.: #8877]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #8877]

Culture medium composition

expand / minimize

[Ref.: #40321]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #40321]

Culture medium growth

[Ref.: #40321]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #8877]	growth	30 °C
[Ref.: #18455]	optimum	28 °C
[Ref.: #40321]	growth	30 °C
[Ref.: #49477]	growth	30-37 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #8877]	Temperature range	mesophilic
[Ref.: #18455]	Temperature range	mesophilic
[Ref.: #40321]	Temperature range	mesophilic
[Ref.: #49477]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #49477]

Oxygen tolerance

aerobe

[Ref.: #8877]	Name of produced compound	methanethiol
[Ref.: #20216]	Name of produced compound	Methanethiol

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #18455]		NaCl		maximum	10	%

[Ref.: #8877]	Murein short key	A31
[Ref.: #8877]	Murein types	A1gamma m-Dpm-direct

[Ref.: #67770]

Observation

quinones: MK-8(H₂), MK-7(H₂)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #18455]	arabinose ChEBI	-
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #18455]	cellulose ChEBI	-
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #18455]	fructose ChEBI	-
[Ref.: #68379]	gelatin ChEBI	+	hydrolysis	* from API Coryne, API 20E
[Ref.: #18455]	glucose ChEBI	+
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68379]	maltose ChEBI	+	fermentation	* from API Coryne
[Ref.: #18455]	mannitol ChEBI	-
[Ref.: #18455]	myo-inositol ChEBI	+
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #18455]	raffinose ChEBI	-
[Ref.: #18455]	rhamnose ChEBI	-
[Ref.: #18455]	sucrose ChEBI	+
[Ref.: #68379]	sucrose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68379]	urea ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
[Ref.: #18455]	xylose ChEBI	-
	Only first 10 entries are displayed. Click here to see all.

	Metabolite
[Ref.: #68368]	acetoin ChEBI	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	* from API 20E
[Ref.: #68368]	indole ChEBI	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68379]	alpha-glucosidase	-	3.2.1.20	* from API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68379]	beta-galactosidase	+	3.2.1.23	* from API Coryne
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym, API 20E
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne
[Ref.: #68379]	beta-glucuronidase	+	3.2.1.31	* from API Coryne
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	+		* from API Coryne, API 20E
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym, API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #68379]	pyrazinamidase	+	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68379]	urease	+	3.5.1.5	* from API Coryne
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #18455]	42	-	-	-	-	+	-	-	-	-	+	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #18455]	130	-	+	+	-	+	+	-	-	-	+	+	not determinedn.d.	-	-	-	+	+	-	+	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #18455]	166	not determinedn.d.	+	+	+	-	+	+	-	-	-	+	+	-	-	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8877]	Sample type/isolated from	cheddar cheese

[Ref.: #49477]	Sample type/isolated from	Cheddar cheese

[Ref.: #67770]	Sample type/isolated from	Cheddar cheese
* marker position based on {}

Isolation sources categories

#Engineered

#Food production

#Dairy product

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18455]	Biosafety level	1
[Ref.: #8877]	Biosafety level	2	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Brevibacterium casei partial 16S rRNA gene, type strain CIP 102111T	AM411119	178	ENA	33889 tax ID	*
[Ref.: #20218]	Brevibacterium casei 16S rRNA gene, strain DSM 20657	AJ251418	1423	ENA	33889 tax ID	*
[Ref.: #20218]	B.casei (NCDO 2048) gene for 16S ribosomal RNA	X76564	1450	ENA	33889 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Brevibacterium casei CIP 102111 CIP 102111	GCA_900169275	contig	GenBank	1255625 tax ID
[Ref.: #66792]	Brevibacterium casei CIP 102111	1255625.3	wgs	PATRIC	1255625 tax ID	*
[Ref.: #66792]	Brevibacterium casei strain FDAARGOS_936 strain Not applicable	33889.20	complete	PATRIC	33889 tax ID	*
[Ref.: #66792]	Brevibacterium casei CIP 102111	2600255381	draft	JGI IMG/M	1255625 tax ID	*

[Ref.: #8877]

GC-content

66.7 mol%

Availability in culture collections External links

[Ref.: #8877]

Culture collection no.

DSM 20657, ATCC 35513, NCDO 2048, CCUG 29001, JCM 2594, BCRC 12214, CCM 4100, CECT 4161, CGMCC 1.1930, CIP 102111, IFO 14812, IMET 10997, KCTC 3082, KCTC 3085, NBRC 14812, NCIMB 702048, VKM Ac-2114

[Ref.: #20218]

Associated Passport(s) in StrainInfo

129349, 110806, 110807

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Brevibacterium ammoniilyticum sp. nov., an ammonia-degrading bacterium isolated from sludge of a wastewater treatment plant.	Kim J, Srinivasan S, You T, Bang JJ, Park S, Lee SS	Int J Syst Evol Microbiol	10.1099/ijs.0.039305-0	2012	*
Metabolism	Efficient utilization of ectoine by halophilic Brevibacterium species and Escherichia coli subjected to osmotic downshock.	Nagata S, Wang C	J Biosci Bioeng	10.1263/jbb.99.61	2005	*
Phylogeny	Brevibacterium celere sp. nov., isolated from degraded thallus of a brown alga.	Ivanova EP, Christen R, Alexeeva YV, Zhukova NV, Gorshkova NM, Lysenko AM, Mikhailov VV, Nicolau DV	Int J Syst Evol Microbiol	10.1099/ijs.0.02867-0	2004	*
Phylogeny	Brevibacterium casei sp. nov. and Brevibacterium epidermidis sp. nov.	Collins MD, Farrow JA, Goodfellow M, Minnikin DE	Syst Appl Microbiol	10.1016/S0723-2020(83)80023-X	1983	*

References

#8877

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20657

#18455

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20216

Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) Curators of the HKI: Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40321 ; Curators of the CIP;
#49477 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 29001

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

* These data were automatically processed and therefore are not curated

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