Bradyrhizobium cytisi (DSM 23720, CECT 7749, LMG 25866)

Name: Bradyrhizobium cytisi (DSM 23720, CECT 7749, LMG 25866)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1817

Type strain

Strain Designation CTAW11

Culture col. no. DSM 23720 CECT 7749 LMG 25866 CTAW11

NCBI tax ID(s) 515489

Special Collections DSMZ Literature strains

History DSMZ <- A. Peix, IRNASA-CSIC, Salamanca, Spain; CTAW11 <- A. Peix Geldart <- R. Chahboune

Links

Bradyrhizobium cytisi CTAW11 is an obligate aerobe, Gram-negative, rod-shaped bacterium that was isolated from effective nodules of Cytisus villosus.

Gram-negative rod-shaped obligate aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 47598

LMG 25866,CECT 7749,DSM 23720

@ref 20215

Last LPSN update 2026-05-29 08:25:21

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Hyphomicrobiales

Family Nitrobacteraceae

Genus Bradyrhizobium

Species Bradyrhizobium cytisi

Full scientific name Bradyrhizobium cytisi Chahboune et al. 2011

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
23331	negative	rod-shaped
125438	negative		97.269

Colony morphology

@ref	Colony size	Incubation period	Medium used
23331	<1 mm	7 days	YMA

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
17353	MODIFIED YMA MEDIUM (DSMZ Medium 1334)		Medium recipe at MediaDive	Name: MODIFIED YMA MEDIUM (DSMZ Medium 1334) Composition: Agar 15.0 g/l Mannitol 7.0 g/l Yeast extract 0.5 g/l MgSO4 x 7 H2O 0.2 g/l K2HPO4 0.2 g/l Distilled water
23331	YMA

Culture temp

@ref	Growth	Type	Temperature (°C)
17353	positive	growth	25
23331	positive	growth	28
23331	negative	growth	4
23331	negative	growth	37

Culture pH

@ref	Ability	Type	PH
23331	positive	growth	6.0-8.0

Physiology and metabolism

Oxygen tolerance

23331

Oxygen toleranceobligate aerobe

Halophily

@ref	Salt	Growth	Tested relation	Concentration
23331	NaCl		growth	0.25 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23331	17128 ChEBI	adipate	+	carbon source
23331	35391 ChEBI	aspartate	+	nitrogen source
23331	17750 ChEBI	betaine	-	builds base from
23331	17750 ChEBI	betaine	+	nitrogen source
23331	17057 ChEBI	cellobiose	-	carbon source
23331	16947 ChEBI	citrate	+/-	carbon source
23331	17108 ChEBI	D-arabinose	+	builds acid from
23331	15824 ChEBI	D-fructose	+	builds acid from
23331	15824 ChEBI	D-fructose	+	carbon source
23331	12936 ChEBI	D-galactose	-	builds acid from
23331	12936 ChEBI	D-galactose	+	carbon source
23331	17634 ChEBI	D-glucose	+	builds acid from
23331	17634 ChEBI	D-glucose	+	carbon source
23331	16024 ChEBI	D-mannose	+	builds acid from
23331	16024 ChEBI	D-mannose	+	carbon source
23331	16988 ChEBI	D-ribose	+	builds acid from
23331	16988 ChEBI	D-ribose	+	carbon source
23331	65327 ChEBI	D-xylose	+	builds acid from
23331	65327 ChEBI	D-xylose	+	carbon source
23331	27689 ChEBI	decanoate	-	carbon source
23331	4853 ChEBI	esculin	+/-	hydrolysis
23331	16813 ChEBI	galactitol	-	carbon source
23331	24265 ChEBI	gluconate	+	carbon source
23331	15428 ChEBI	glycine	-	nitrogen source
23331	30849 ChEBI	L-arabinose	+	carbon source
23331	29991 ChEBI	L-aspartate	+	builds base from
23331	29985 ChEBI	L-glutamate	+	builds base from
23331	29985 ChEBI	L-glutamate	+	nitrogen source
23331	17203 ChEBI	L-proline	-	builds base from
23331	17203 ChEBI	L-proline	+	nitrogen source
23331	62345 ChEBI	L-rhamnose	-	carbon source
23331	17115 ChEBI	L-serine	-	builds base from
23331	17115 ChEBI	L-serine	+	nitrogen source
23331	17266 ChEBI	L-sorbose	-	carbon source
23331	17716 ChEBI	lactose	-	carbon source
23331	25115 ChEBI	malate	+/-	carbon source
23331	17306 ChEBI	maltose	-	carbon source
23331	29864 ChEBI	mannitol	+	carbon source
23331	29864 ChEBI	mannitol	+/-	builds acid from
23331	506227 ChEBI	N-acetylglucosamine	+	carbon source
23331	17632 ChEBI	nitrate	-	reduction
23331	18401 ChEBI	phenylacetate	-	carbon source
23331	15361 ChEBI	pyruvate	-	builds base from
23331	15361 ChEBI	pyruvate	+/-	carbon source
23331	16634 ChEBI	raffinose	-	carbon source
23331	15963 ChEBI	ribitol	-	builds acid from
23331	15963 ChEBI	ribitol	+	carbon source
23331	15611 ChEBI	sarcosine	-	nitrogen source
23331	17992 ChEBI	sucrose	-	carbon source
23331	27082 ChEBI	trehalose	-	carbon source

Antibiotic resistance

@ref	ChEBI	Metabolite	Resistance conc.	Sensitivity conc.
23331	28971	ampicillin	2 µg
23331	3515	cefuroxime		30 µg
23331	100241	ciprofloxacin	5 µg
23331	49566	cloxacillin	1 µg
23331	48923	erythromycin	2 µg
23331	17833	gentamicin		10 µg
23331	7507	neomycin		5 µg
23331	17334	penicillin	10 Unit
23331	8309	polymyxin b	300 Unit
23331	27902	tetracycline		30 µg

Enzymes

@ref	Value	Activity	Ec
23331	arginine dihydrolase	-	3.5.3.6
23331	beta-galactosidase	-	3.2.1.23
23331	urease	-	3.5.1.5

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Shrub (Scrub)
#Host Body-Site	#Plant	#Root nodule

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent
17353	effective nodules of Cytisus villosus	Cytisus villosus	Moroquian rif, Awdal region	Morocco	MAR	Africa

Taxonmaps

69479

Global distribution of 16S sequence EU561065 (>99% sequence identity) for Bradyrhizobium from Microbeatlas

Aquatic Samples	2827
Soil Samples	5001
Animal Samples	4320
Plant Samples	739
Total Samples	12887

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
17353	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM812351v1 assembly for Bradyrhizobium cytisi CTAW11	contig	GCA_008123515	515489.3	8112052170	515489	39.58

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
17353	Bradyrhizobium cytisi strain CTAW11 from Morocco 16S ribosomal RNA gene, partial sequence	EU561065	1516		515489

GC content

@ref	GC-content (mol%)	Method
17353	65.1	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Bradyrhizobium cytisi CTAW11
NCBI: GCA_008123515.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	97.27	yes
125438	anaerobic	anaerobicⓘ	no	92.49	yes
125438	aerobic	aerobicⓘ	yes	76.64	no
125438	spore-forming	spore-formingⓘ	no	87.11	no
125438	thermophilic	thermophileⓘ	no	97.49	yes
125438	flagellated	motile2+ⓘ	yes	75.48	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Astragalus algarbiensis is nodulated by the genistearum symbiovar of Bradyrhizobium spp. in Morocco.	Alami S, Lamin H, Bouhnik O, El Faik S, Filali-Maltouf A, Abdelmoumen H, Bedmar EJ, Missbah El Idrissi M	Syst Appl Microbiol	10.1016/j.syapm.2019.03.004	2019
Phylogeny	Bradyrhizobium hipponense sp. nov., isolated from Lupinus angustifolius growing in the northern region of Tunisia.	Rejili M, Off K, Brachmann A, Marin M	Int J Syst Evol Microbiol	10.1099/ijsem.0.004445	2020
Phylogeny	Bradyrhizobium rifense sp. nov. isolated from effective nodules of Cytisus villosus grown in the Moroccan Rif.	Chahboune R, Carro L, Peix A, Ramirez-Bahena MH, Barrijal S, Velazquez E, Bedmar EJ	Syst Appl Microbiol	10.1016/j.syapm.2012.06.001	2012
Phylogeny	Bradyrhizobium cytisi sp. nov., isolated from effective nodules of Cytisus villosus.	Chahboune R, Carro L, Peix A, Barrijal S, Velazquez E, Bedmar EJ	Int J Syst Evol Microbiol	10.1099/ijs.0.027649-0	2011

References

#17353	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23720
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#23331	Rajaa Chahboune, Lorena Carro, Alvaro Peix, Said Barrijal, Encarna Velázquez, Eulogio J. Bedmar: Bradyrhizobium cytisi sp. nov., isolated from effective nodules of Cytisus villosus. IJSEM 61: 2922 - 2927 2011 ( DOI 10.1099/ijs.0.027649-0 , PubMed 21257682 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Bradyrhizobium cytisi (DSM 23720, CECT 7749, LMG 25866)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Halophily

Metabolite utilization

Antibiotic resistance

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_008123515

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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