Name: Bradyrhizobium cytisi CTAW11
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1817

Type strain:

Species: Bradyrhizobium cytisi

Strain Designation: CTAW11

Culture col. no.: DSM 23720, CECT 7749, LMG 25866

Strain history: <- A. Peix, IRNASA-CSIC, Salamanca, Spain; CTAW11 <- A. Peix Geldart <- R. Chahboune

NCBI tax ID(s): 515489 (species)

SI-ID 47598

LMG 25866, CECT 7749, DSM 23720

Section

Bradyrhizobium cytisi CTAW11 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from effective nodules of Cytisus villosus.

Gram-negative
rod-shaped
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Nitrobacteraceae*
	Genus	*Bradyrhizobium*
	Species	**Bradyrhizobium cytisi**
	Full Scientific Name (LPSN)	Bradyrhizobium cytisi Chahboune et al. 2011

Information on morphological and physiological properties Morphology

[Ref.: #23331]	Gram stain	negative

[Ref.: #23331]	Cell shape	rod-shaped

[Ref.: #23331]	Colony size	<1 mm
[Ref.: #23331]	Incubation period	7 days
[Ref.: #23331]	Cultivation medium used	YMA

Information on culture and growth conditions Culture and growth conditions

[Ref.: #17353]

Culture medium

MODIFIED YMA MEDIUM (DSMZ Medium 1334)

[Ref.: #17353]

Culture medium growth

[Ref.: #17353]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17353]

Culture medium composition

expand / minimize

[Ref.: #23331]

Culture medium

YMA

[Ref.: #23331]

Culture medium growth

	Kind of temperature		Temperature
[Ref.: #17353]		growth	25 °C
[Ref.: #23331]		growth	28 °C
[Ref.: #23331]	no	growth	4 °C
[Ref.: #23331]	no	growth	37 °C

[Ref.: #17353]	Temperature range	mesophilic
[Ref.: #23331]	Temperature range	mesophilic
[Ref.: #23331]	Temperature range	psychrophilic
[Ref.: #23331]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #23331]			growth	6.0-8.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23331]

Oxygen tolerance

obligate aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23331]		NaCl	no	growth	0.25	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23331]	adipate ChEBI	+	carbon source
[Ref.: #23331]	aspartate ChEBI	+	nitrogen source
[Ref.: #23331]	betaine ChEBI	-	builds base from
[Ref.: #23331]	betaine ChEBI	+	nitrogen source
[Ref.: #23331]	cellobiose ChEBI	-	carbon source
[Ref.: #23331]	citrate ChEBI	+/-	carbon source
[Ref.: #23331]	D-arabinose ChEBI	+	builds acid from
[Ref.: #23331]	D-fructose ChEBI	+	builds acid from
[Ref.: #23331]	D-fructose ChEBI	+	carbon source
[Ref.: #23331]	D-galactose ChEBI	-	builds acid from
[Ref.: #23331]	D-galactose ChEBI	+	carbon source
[Ref.: #23331]	D-glucose ChEBI	+	builds acid from
[Ref.: #23331]	D-glucose ChEBI	+	carbon source
[Ref.: #23331]	D-mannose ChEBI	+	builds acid from
[Ref.: #23331]	D-mannose ChEBI	+	carbon source
[Ref.: #23331]	D-ribose ChEBI	+	builds acid from
[Ref.: #23331]	D-ribose ChEBI	+	carbon source
[Ref.: #23331]	D-xylose ChEBI	+	builds acid from
[Ref.: #23331]	D-xylose ChEBI	+	carbon source
[Ref.: #23331]	decanoate ChEBI	-	carbon source
[Ref.: #23331]	esculin ChEBI	+/-	hydrolysis
[Ref.: #23331]	galactitol ChEBI	-	carbon source
[Ref.: #23331]	gluconate ChEBI	+	carbon source
[Ref.: #23331]	glycine ChEBI	-	nitrogen source
[Ref.: #23331]	L-arabinose ChEBI	+	carbon source
[Ref.: #23331]	L-aspartate ChEBI	+	builds base from
[Ref.: #23331]	L-glutamate ChEBI	+	builds base from
[Ref.: #23331]	L-glutamate ChEBI	+	nitrogen source
[Ref.: #23331]	L-proline ChEBI	-	builds base from
[Ref.: #23331]	L-proline ChEBI	+	nitrogen source
[Ref.: #23331]	L-rhamnose ChEBI	-	carbon source
[Ref.: #23331]	L-serine ChEBI	-	builds base from
[Ref.: #23331]	L-serine ChEBI	+	nitrogen source
[Ref.: #23331]	L-sorbose ChEBI	-	carbon source
[Ref.: #23331]	lactose ChEBI	-	carbon source
[Ref.: #23331]	malate ChEBI	+/-	carbon source
[Ref.: #23331]	maltose ChEBI	-	carbon source
[Ref.: #23331]	mannitol ChEBI	+	carbon source
[Ref.: #23331]	mannitol ChEBI	+/-	builds acid from
[Ref.: #23331]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #23331]	nitrate ChEBI	-	reduction
[Ref.: #23331]	phenylacetate ChEBI	-	carbon source
[Ref.: #23331]	pyruvate ChEBI	-	builds base from
[Ref.: #23331]	pyruvate ChEBI	+/-	carbon source
[Ref.: #23331]	raffinose ChEBI	-	carbon source
[Ref.: #23331]	ribitol ChEBI	-	builds acid from
[Ref.: #23331]	ribitol ChEBI	+	carbon source
[Ref.: #23331]	sarcosine ChEBI	-	nitrogen source
[Ref.: #23331]	sucrose ChEBI	-	carbon source
[Ref.: #23331]	trehalose ChEBI	-	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #23331]	ampicillin ChEBI				yes	2	µg
[Ref.: #23331]	cefuroxime ChEBI	yes	30	µg
[Ref.: #23331]	ciprofloxacin ChEBI				yes	5	µg
[Ref.: #23331]	cloxacillin ChEBI				yes	1	µg
[Ref.: #23331]	erythromycin ChEBI				yes	2	µg
[Ref.: #23331]	gentamicin ChEBI	yes	10	µg
[Ref.: #23331]	neomycin ChEBI	yes	5	µg
[Ref.: #23331]	penicillin ChEBI				yes	10	Unit
[Ref.: #23331]	polymyxin b ChEBI				yes	300	Unit
[Ref.: #23331]	tetracycline ChEBI	yes	30	µg

	Enzyme	Enzyme activity	EC number
[Ref.: #23331]	arginine dihydrolase	-	3.5.3.6
[Ref.: #23331]	beta-galactosidase	-	3.2.1.23
[Ref.: #23331]	urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #17353]	Sample type/isolated from	effective nodules of Cytisus villosus
[Ref.: #17353]	Host species	Cytisus villosus
[Ref.: #17353]	Geographic location (country and/or sea, region)	Moroquian rif, Awdal region
[Ref.: #17353]	Country	Morocco
[Ref.: #17353]	Country ISO 3 Code	MAR
[Ref.: #17353]	Continent	Africa
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Shrub (Scrub)
#Host Body-Site	#Plant	#Root nodule

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EU561065 (>99% sequence identity) for Bradyrhizobium from Microbeatlas

Aquatic Samples	2827
Soil Samples	5001
Animal Samples	4320
Plant Samples	739
Total Samples	12887

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #17353]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #17353]	Bradyrhizobium cytisi strain CTAW11 from Morocco 16S ribosomal RNA gene, partial sequence	EU561065	1516	ENA	515489 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bradyrhizobium cytisi CTAW11	GCA_008123515	contig	GenBank	515489 tax ID	*

[Ref.: #17353]

GC-content

65.1 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bradyrhizobium cytisi CTAW11 NCBI: GCA_008123515.1
[Ref.: #69480]	motile	yes	86.547	no
[Ref.: #69480]	flagellated	no	69.124	no
[Ref.: #69480]	gram-positive	no	98.158	yes
[Ref.: #69480]	anaerobic	no	97.796	yes
[Ref.: #69480]	aerobic	yes	87.998	no
[Ref.: #69480]	halophile	no	95.56	no
[Ref.: #69480]	spore-forming	no	93.97	no
[Ref.: #69480]	glucose-util	yes	87.406	no
[Ref.: #69480]	thermophile	no	99.31	yes
[Ref.: #69480]	glucose-ferment	no	89.666	no

Availability in culture collections External links

[Ref.: #17353]

Culture collection no.

DSM 23720, CECT 7749, LMG 25866

[Ref.: #71463]

SI-ID 47598

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bradyrhizobium hipponense sp. nov., isolated from Lupinus angustifolius growing in the northern region of Tunisia.	Rejili M, Off K, Brachmann A, Marin M	Int J Syst Evol Microbiol	10.1099/ijsem.0.004445	2020	*
Phylogeny	Astragalus algarbiensis is nodulated by the genistearum symbiovar of Bradyrhizobium spp. in Morocco.	Alami S, Lamin H, Bouhnik O, El Faik S, Filali-Maltouf A, Abdelmoumen H, Bedmar EJ, Missbah El Idrissi M	Syst Appl Microbiol	10.1016/j.syapm.2019.03.004	2019	*
Phylogeny	Bradyrhizobium rifense sp. nov. isolated from effective nodules of Cytisus villosus grown in the Moroccan Rif.	Chahboune R, Carro L, Peix A, Ramirez-Bahena MH, Barrijal S, Velazquez E, Bedmar EJ	Syst Appl Microbiol	10.1016/j.syapm.2012.06.001	2012	*
Phylogeny	Bradyrhizobium cytisi sp. nov., isolated from effective nodules of Cytisus villosus.	Chahboune R, Carro L, Peix A, Barrijal S, Velazquez E, Bedmar EJ	Int J Syst Evol Microbiol	10.1099/ijs.0.027649-0	2011	*

References

#17353

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23720

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23331

Rajaa Chahboune, Lorena Carro, Alvaro Peix, Said Barrijal, Encarna Velázquez, Eulogio J. Bedmar: Bradyrhizobium cytisi sp. nov., isolated from effective nodules of Cytisus villosus. IJSEM 61: 2922 - 2927 2011 ( DOI 10.1099/ijs.0.027649-0 , PubMed 21257682 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#71463

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID47598.1 )

* These data were automatically processed and therefore are not curated

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