Bradyrhizobium pachyrhizi PAC48 | Type strain | DSM 19631, CECT 7396, LMG 24246

Strain identifier

BacDive ID: 1815

Type strain:

Species: Bradyrhizobium pachyrhizi

Strain Designation: PAC48

Culture col. no.: DSM 19631, CECT 7396, LMG 24246

Section

Name and taxonomic classification
Morphology and physiology
Culture and growth conditions
Isolation, sampling and environmental information
Application and interaction
Molecular biology
Strain availability

External links

Information on the name and the taxonomic classification.Name and taxonomic classification

	Last LPSN update	31-03-2020 (DD-MM-YYYY)
	Domain	Bacteria
	Phylum	Proteobacteria
	Class	Alphaproteobacteria
	Order	Rhizobiales
	Family	Bradyrhizobiaceae
	Genus	Bradyrhizobium
	Species	Bradyrhizobium pachyrhizi
	Full Scientific Name (PNU)	Bradyrhizobium pachyrhizi Ramírez-Bahena et al. 2009

Information on morphological and physiological propertiesMorphology and physiology

[Ref.: #23330]	Gram stain	negative
[Ref.: #23330]	Cell shape	rod-shaped

[Ref.: #23330]	Colony color	pearl white
[Ref.: #23330]	Cultivation medium used	YMA

	Enzyme	Enzyme activity	EC number
[Ref.: #23330]	arginine dihydrolase	-	3.5.3.6
[Ref.: #23330]	beta-galactosidase	-	3.2.1.23
[Ref.: #23330]	urease	+/-	3.5.1.5

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23330]		NaCl	no	growth	1	%

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic intermediate	Concentration		Antibiotic resistance	Concentration
[Ref.: #23330]	Ampicillin ChEBI				yes	1	µg/mL
[Ref.: #23330]	Cefuroxime ChEBI							yes	15	µg/mL
[Ref.: #23330]	Ciprofloxacin ChEBI							yes	5	µg/mL
[Ref.: #23330]	Cloxacillin ChEBI				yes	1	µg/mL
[Ref.: #23330]	Erythromycin ChEBI							yes	50	µg/mL
[Ref.: #23330]	Gentamicin ChEBI	yes	20	µg/mL
[Ref.: #23330]	Kanamycin ChEBI	yes		µg/mL
[Ref.: #23330]	Neomycin ChEBI	yes	5	µg/mL
[Ref.: #23330]	Penicillin ChEBI							yes	2	µg/mL
[Ref.: #23330]	Polymyxin B ChEBI							yes	300	µg/mL
[Ref.: #23330]	Tetracycline ChEBI							yes	15	µg/mL
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23330]	Adipic acid ChEBI	+	carbon source
[Ref.: #23330]	Caprate ChEBI	-	carbon source
[Ref.: #23330]	Cellobiose ChEBI	-	carbon source
[Ref.: #23330]	Citric acid ChEBI	-	carbon source
[Ref.: #23330]	D-Xylose ChEBI	+	builds acid from
[Ref.: #23330]	Esculin ChEBI	+/-	hydrolysis
[Ref.: #23330]	Fructose ChEBI	+	builds acid from
[Ref.: #23330]	Gluconate ChEBI	+	carbon source
[Ref.: #23330]	Glucose ChEBI	+	carbon source
[Ref.: #23330]	Glycine ChEBI	+/-	nitrogen source
[Ref.: #23330]	L-Arabinose ChEBI	+	carbon source
[Ref.: #23330]	L-Malate ChEBI	+/-	carbon source
[Ref.: #23330]	L-Proline ChEBI	+	nitrogen source
[Ref.: #23330]	L-Rhamnose ChEBI	-	builds acid from
[Ref.: #23330]	L-Rhamnose ChEBI	-	builds base from
[Ref.: #23330]	L-Rhamnose ChEBI	+	carbon source
[Ref.: #23330]	L-Sorbose ChEBI	-	builds acid from
[Ref.: #23330]	L-Sorbose ChEBI	-	builds base from
[Ref.: #23330]	L-Sorbose ChEBI	+	carbon source
[Ref.: #23330]	Lactic acid ChEBI	+	carbon source
[Ref.: #23330]	Lactose ChEBI	-	builds acid from
[Ref.: #23330]	Maltose ChEBI	+/-	carbon source
[Ref.: #23330]	Mannitol ChEBI	-	builds base from
[Ref.: #23330]	Mannitol ChEBI	+	carbon source
[Ref.: #23330]	Mannose ChEBI	-	carbon source
[Ref.: #23330]	Melezitose ChEBI	+/-	carbon source
[Ref.: #23330]	N-Acetylglucosamine ChEBI	-	carbon source
[Ref.: #23330]	Nitrate ChEBI	+	reduction
[Ref.: #23330]	Phenylacetic acid ChEBI	-	carbon source
[Ref.: #23330]	Pyruvic acid ChEBI	+	carbon source
[Ref.: #23330]	Succinic acid ChEBI	+	carbon source
[Ref.: #23330]	Trehalose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

[Ref.: #23330]

Oxygen tolerance

obligate aerobe

Information on culture and growth conditionsCulture and growth conditions

[Ref.: #8221]

Culture medium

RHIZOBIUM MEDIUM (DSMZ Medium 98), 28°C

[Ref.: #8221]

Culture medium growth

[Ref.: #8221]

Culture medium link

medium recipe provided by DSMZ

[Ref.: #23330]

Culture medium

YMA

[Ref.: #23330]

Culture medium growth

		Temperature
[Ref.: #8221]	growth	28 ̊C
[Ref.: #23330]	growth	5-37 ̊C
[Ref.: #23330]	optimum	28 ̊C

[Ref.: #8221]	Temperature range	mesophilic
[Ref.: #23330]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #23330]			optimum	7.0
[Ref.: #23330]			growth	4.5-8.0

Information on isolation source, the sampling and environmental conditionsIsolation, sampling and environmental information

[Ref.: #8221]

Sample type/isolated from

nodules of Pachyrhizus erosus

[Ref.: #8221]

Host species

Pachyrhizus erosus

[Ref.: #8221]

Geographic location (country and/or sea, region)

Guanacaste

[Ref.: #8221]

Country

Costa Rica

[Ref.: #8221]

Continent

Middle and South America

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root nodule

What are isolation sources categories?

* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hostsApplication and interaction

[Ref.: #8221]

Biosafety level

Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databassMolecular biology

[Ref.: #8221]	GC-content	63.5 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #23330]	GC-content	63.5 mol%	thermal denaturation, midpoint method (Tm)

Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence database	Sequence accession description	Sequence accession number	Sequence type	Sequence length (bp)	Associated NCBI tax ID
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Bradyrhizobium pachyrhizi strain LMG 24246 nitrogenase reductase (nifH) gene, partial cds	HM047124 ENA		711	280333 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Bradyrhizobium pachyrhizi strain LMG 24246 nodulation protein C (nodC) gene, partial cds	HM047128 ENA		589	280333 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Bradyrhizobium pachyrhizi strain LMG 24246 recombinase A (recA) gene, partial cds	HM047130 ENA		487	280333 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Bradyrhizobium pachyrhizi strain LMG 24246 recombination protein A (recA) gene, partial cds	HQ587416 ENA		463	280333 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Bradyrhizobium pachyrhizi strain LMG 24246 RNA polymerase subunit beta (rpoB) gene, partial cds	HQ587648 ENA		771	280333 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Bradyrhizobium pachyrhizi strain LMG 24246 N-acetylglucosaminyltransferase (nodC) gene, partial cds	HQ588110 ENA		728	280333 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Bradyrhizobium pachyrhizi strain LMG 24246 DNA gyrase subunit beta (gyrB) gene, partial cds	HQ873310 ENA		622	280333 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Bradyrhizobium pachyrhizi strain LMG 24246 Hsp70 class chaperone (dnaK) gene, partial cds	JF308946 ENA		369	280333 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Bradyrhizobium pachyrhizi hypothetical protein (SMc00019) gene, partial cds	JX064210 ENA		309	280333 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Bradyrhizobium pachyrhizi TruA (truA) gene, partial cds	JX064282 ENA		492	280333 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Bradyrhizobium pachyrhizi ThrA (thrA) gene, partial cds	JX064354 ENA		491	280333 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Bradyrhizobium pachyrhizi acyltransferase nodulation protein A (nodA) gene, partial cds	KC509198 ENA		465	280333 tax ID	*
[Ref.: #8221]	16S rRNA gene, Marker Gene (GenBank Direct submission)	Bradyrhizobium pachyrhizi strain PAC48 16S ribosomal RNA gene, partial sequence	AY624135 ENA	16S	1483	280333 tax ID

Availability in culture collectionsStrain availability

[Ref.: #8221]

Culture collection no.

DSM 19631, CECT 7396, LMG 24246

[Ref.: #8221]

Strain history

<- E. Velázquez, Depart. Microbiol. y Gen., Univ. Salamanca, Spain <- M. Grum; PAC48

[Ref.: #20218]

Associated Passport(s) in StrainInfo

844819, 844474, 720243

References

#8221

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19631

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23330

Martha Helena Ram?rez-Bahena, Alvaro Peix, Ra?l Rivas, Mar?a Camacho, Dulce N. Rodr?guez-Navarro, Pedro F. Mateos, Eustoquio Mart?nez-Molina, Anne Willems, Encarna Vel?zquez: Bradyrhizobium pachyrhizi sp. nov. and Bradyrhizobium jicamae sp. nov., isolated from effective nodules of Pachyrhizus erosus. IJSEM 59: 1929 - 1934 2009 ( DOI 10.1099/ijs.0.006320-0 )

* These References are textmined

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