Name: Methanocalculus pumilus MHT-1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 18142

Type strain:

Species: Methanocalculus pumilus

Strain Designation: MHT-1

Culture col. no.: DSM 12632, JCM 10627, ATCC BAA-129

Strain history: M. Hatsu HMT-1.

NCBI tax ID(s): 152007 (species)

SI-ID 49331

DSM 12632, JCM 10627, ATCC BAA-129

Section

Methanocalculus pumilus MHT-1 is an anaerobe, mesophilic archaeon that was isolated from leachate from waste-disposal site.

anaerobe
mesophilic
16S sequence
Archaea

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	"Methanomicrobia"
	Order	*Methanomicrobiales*
	Family	*Methanocalculaceae*
	Genus	*Methanocalculus*
	Species	**Methanocalculus pumilus**
	Full Scientific Name (LPSN)	Methanocalculus pumilus Mori et al. 2000

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4774]

Culture medium

METHANOCALCULUS PUMILUS MEDIUM (DSMZ Medium 892)

[Ref.: #4774]

Culture medium growth

[Ref.: #4774]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4774]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #4774]			growth	37 °C
[Ref.: #67770]			growth	35 °C

[Ref.: #4774]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #4774]

Oxygen tolerance

anaerobe

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4774]	Sample type/isolated from	leachate from waste-disposal site
[Ref.: #4774]	Country	Japan
[Ref.: #4774]	Country ISO 3 Code	JPN
[Ref.: #4774]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Leachate of waste disposal site
[Ref.: #67770]	Geographic location (country and/or sea, region)	port of Osaka
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered

#Waste

#Wastewater

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4774]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #4774]	Methanocalculus pumilus gene for 16S rRNA, partial sequence	AB008853	1436	ENA	152007 tax ID
[Ref.: #67770]	Methanobacterium thermoautotrophicum gene for 16S rRNA, partial sequence	AB020530	1327	ENA	145262 tax ID

[Ref.: #4774]	GC-content	51.9 mol%
[Ref.: #67770]	GC-content	51.9 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #4774]

Culture collection no.

DSM 12632, JCM 10627, ATCC BAA-129

[Ref.: #87128]

SI-ID 49331

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methanocalculus pumilus sp. nov., a heavy-metal-tolerant methanogen isolated from a waste-disposal site.	Mori K, Yamamoto H, Kamagata Y, Hatsu M, Takamizawa K	Int J Syst Evol Microbiol	10.1099/00207713-50-5-1723	2000	*

References

#4774

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 12632

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#87128

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID49331.1 )

* These data were automatically processed and therefore are not curated

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