Name: Rummeliibacillus stabekisii KSC-SF6g
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 18132

Type strain:

Species: Rummeliibacillus stabekisii

Strain Designation: KSC-SF6g

Culture col. no.: DSM 25578, NBRC 104870, NRRL B-51320

Strain history: <- NRRL; NRRL-B-51320 <- K. Venkateswaran, Jet Propulsion Laboratory, NASA; KSC-SF6g

NCBI tax ID(s): 241244 (species)

Other strains from Rummeliibacillus stabekisii

R. stabekisii DSM 21017, NBRC 12622, ATU B-205-e
R. stabekisii DSM 100504, TMCC 8453

Section

Rummeliibacillus stabekisii KSC-SF6g is an aerobe, spore-forming, mesophilic bacterium that was isolated from Payload Hazardous Servicing Facility, clean room floor.

Gram-positive
motile
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Caryophanaceae*
	Genus	*Rummeliibacillus*
	Species	**Rummeliibacillus stabekisii**
	Full Scientific Name (PNU)	Rummeliibacillus stabekisii Vaishampayan et al. 2009

Information on morphological and physiological properties Morphology

[Ref.: #29095]	Gram stain	positive
[Ref.: #29095]	Cell length	2.98 µm
[Ref.: #29095]	Cell width	1.105 µm
[Ref.: #29095]	Cell shape	rod-shaped
[Ref.: #29095]	Motility	yes

[Ref.: #29095]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #18050]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #18050]

Culture medium growth

[Ref.: #18050]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #18050]

Culture medium composition

expand / minimize

[Ref.: #37908]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37908]

Culture medium growth

[Ref.: #37908]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #18050]	growth	30 °C
[Ref.: #29095]	growth	28-32 °C
[Ref.: #29095]	optimum	30 °C
[Ref.: #37908]	growth	30 °C

[Ref.: #18050]	Temperature range	mesophilic
[Ref.: #29095]	Temperature range	mesophilic
[Ref.: #37908]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #29095]

Oxygen tolerance

aerobe

[Ref.: #29095]

Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #29095]		NaCl		growth	<7	%
[Ref.: #29095]		NaCl		optimum	3.5	%

[Ref.: #18050]	Murein short key	A11.31
[Ref.: #18050]	Murein types	A4alpha L-Lys-D-Asp

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #29095]	arabinose ChEBI	+	carbon source
[Ref.: #29095]	cellobiose ChEBI	+	carbon source
[Ref.: #29095]	citrate ChEBI	+	carbon source
[Ref.: #29095]	galactose ChEBI	+	carbon source
[Ref.: #29095]	glucose ChEBI	+	carbon source
[Ref.: #29095]	lactose ChEBI	+	carbon source
[Ref.: #29095]	mannitol ChEBI	+	carbon source
[Ref.: #29095]	mannose ChEBI	+	carbon source
[Ref.: #29095]	ribose ChEBI	+	carbon source
[Ref.: #29095]	xylose ChEBI	+	carbon source

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	formaldehyde oxidation	100	3 of 3
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	biotin biosynthesis	100	4 of 4
[Ref.: #66794]	acetate fermentation	100	4 of 4
[Ref.: #66794]	teichoic acid biosynthesis	100	1 of 1
[Ref.: #66794]	vitamin K metabolism	100	5 of 5
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	lipoate biosynthesis	100	5 of 5
[Ref.: #66794]	palmitate biosynthesis	95.45	21 of 22
[Ref.: #66794]	vitamin B1 metabolism	92.31	12 of 13
[Ref.: #66794]	propionate fermentation	90	9 of 10
[Ref.: #66794]	aspartate and asparagine metabolism	88.89	8 of 9
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	C4 and CAM-carbon fixation	87.5	7 of 8
[Ref.: #66794]	gluconeogenesis	87.5	7 of 8
[Ref.: #66794]	propanol degradation	85.71	6 of 7
[Ref.: #66794]	glutamate and glutamine metabolism	82.14	23 of 28
[Ref.: #66794]	pentose phosphate pathway	81.82	9 of 11
[Ref.: #66794]	pyrimidine metabolism	80	36 of 45
[Ref.: #66794]	cellulose degradation	80	4 of 5
[Ref.: #66794]	metabolism of amino sugars and derivatives	80	4 of 5
[Ref.: #66794]	glycine betaine biosynthesis	80	4 of 5
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	purine metabolism	79.79	75 of 94
[Ref.: #66794]	citric acid cycle	78.57	11 of 14
[Ref.: #66794]	photosynthesis	78.57	11 of 14
[Ref.: #66794]	serine metabolism	77.78	7 of 9
[Ref.: #66794]	CO2 fixation in Crenarchaeota	77.78	7 of 9
[Ref.: #66794]	sulfopterin metabolism	75	3 of 4
[Ref.: #66794]	ketogluconate metabolism	75	6 of 8
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
[Ref.: #66794]	ppGpp biosynthesis	75	3 of 4
[Ref.: #66794]	flavin biosynthesis	73.33	11 of 15
[Ref.: #66794]	NAD metabolism	72.22	13 of 18
[Ref.: #66794]	cardiolipin biosynthesis	71.43	5 of 7
[Ref.: #66794]	threonine metabolism	70	7 of 10
[Ref.: #66794]	urea cycle	69.23	9 of 13
[Ref.: #66794]	phenylalanine metabolism	69.23	9 of 13
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	lysine metabolism	66.67	28 of 42
[Ref.: #66794]	valine metabolism	66.67	6 of 9
[Ref.: #66794]	acetyl CoA biosynthesis	66.67	2 of 3
[Ref.: #66794]	enterobactin biosynthesis	66.67	2 of 3
[Ref.: #66794]	alanine metabolism	65.52	19 of 29
[Ref.: #66794]	methionine metabolism	65.38	17 of 26
[Ref.: #66794]	glycolysis	64.71	11 of 17
[Ref.: #66794]	lipid metabolism	64.52	20 of 31
[Ref.: #66794]	isoprenoid biosynthesis	61.54	16 of 26
[Ref.: #66794]	leucine metabolism	61.54	8 of 13
[Ref.: #66794]	cysteine metabolism	61.11	11 of 18
[Ref.: #66794]	3-chlorocatechol degradation	60	3 of 5
[Ref.: #66794]	phenylacetate degradation (aerobic)	60	3 of 5
[Ref.: #66794]	factor 420 biosynthesis	60	3 of 5
[Ref.: #66794]	methylglyoxal degradation	60	3 of 5
[Ref.: #66794]	non-pathway related	57.89	22 of 38
[Ref.: #66794]	glutathione metabolism	57.14	8 of 14
[Ref.: #66794]	heme metabolism	57.14	8 of 14
[Ref.: #66794]	tetrahydrofolate metabolism	57.14	8 of 14
[Ref.: #66794]	allantoin degradation	55.56	5 of 9
[Ref.: #66794]	tryptophan metabolism	55.26	21 of 38
[Ref.: #66794]	proline metabolism	54.55	6 of 11
[Ref.: #66794]	oxidative phosphorylation	53.85	49 of 91
[Ref.: #66794]	histidine metabolism	51.72	15 of 29
[Ref.: #66794]	arginine metabolism	50	12 of 24
[Ref.: #66794]	glycine metabolism	50	5 of 10
[Ref.: #66794]	pantothenate biosynthesis	50	3 of 6
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	starch degradation	50	5 of 10
[Ref.: #66794]	aminopropanol phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	glycolate and glyoxylate degradation	50	3 of 6
[Ref.: #66794]	selenocysteine biosynthesis	50	3 of 6
[Ref.: #66794]	glycogen biosynthesis	50	2 of 4
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	50	6 of 12
[Ref.: #66794]	cis-vaccenate biosynthesis	50	1 of 2
[Ref.: #66794]	isoleucine metabolism	50	4 of 8
[Ref.: #66794]	Entner Doudoroff pathway	50	5 of 10
[Ref.: #66794]	d-mannose degradation	44.44	4 of 9
[Ref.: #66794]	ginsenoside metabolism	43.75	7 of 16
[Ref.: #66794]	ubiquinone biosynthesis	42.86	3 of 7
[Ref.: #66794]	ascorbate metabolism	40.91	9 of 22
[Ref.: #66794]	creatinine degradation	40	2 of 5
[Ref.: #66794]	coenzyme M biosynthesis	40	4 of 10
[Ref.: #66794]	gallate degradation	40	2 of 5
[Ref.: #66794]	cholesterol biosynthesis	36.36	4 of 11
[Ref.: #66794]	metabolism of disaccharids	36.36	4 of 11
[Ref.: #66794]	degradation of pentoses	35.71	10 of 28
[Ref.: #66794]	tyrosine metabolism	35.71	5 of 14
[Ref.: #66794]	phenol degradation	35	7 of 20
[Ref.: #66794]	polyamine pathway	34.78	8 of 23
[Ref.: #66794]	cyanate degradation	33.33	1 of 3
[Ref.: #66794]	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
[Ref.: #66794]	octane oxidation	33.33	1 of 3
[Ref.: #66794]	lipid A biosynthesis	33.33	3 of 9
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	vitamin B12 metabolism	32.35	11 of 34
[Ref.: #66794]	degradation of sugar acids	32	8 of 25
[Ref.: #66794]	degradation of sugar alcohols	31.25	5 of 16
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	30.77	4 of 13
[Ref.: #66794]	phenylpropanoid biosynthesis	30.77	4 of 13
[Ref.: #66794]	4-hydroxyphenylacetate degradation	30	3 of 10
[Ref.: #66794]	reductive acetyl coenzyme A pathway	28.57	2 of 7
[Ref.: #66794]	arachidonic acid metabolism	27.78	5 of 18
[Ref.: #66794]	vitamin B6 metabolism	27.27	3 of 11
[Ref.: #66794]	CMP-KDO biosynthesis	25	1 of 4
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	androgen and estrogen metabolism	25	4 of 16
[Ref.: #66794]	butanoate fermentation	25	1 of 4
[Ref.: #66794]	sulfate reduction	23.08	3 of 13
[Ref.: #66794]	4-hydroxymandelate degradation	22.22	2 of 9
[Ref.: #66794]	nitrate assimilation	22.22	2 of 9
[Ref.: #66794]	degradation of hexoses	22.22	4 of 18
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #18050]	Sample type/isolated from	Payload Hazardous Servicing Facility, clean room floor
[Ref.: #18050]	Geographic location (country and/or sea, region)	FL, Kennedy Space Center
[Ref.: #18050]	Country	USA
[Ref.: #18050]	Country ISO 3 Code	USA
[Ref.: #18050]	Continent	North America
* marker position based on {}

Isolation sources categories

#Engineered

#Built environment

#Clean room

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence DQ870754 (>99% sequence identity) for Rummeliibacillus stabekisii subclade from Microbeatlas

Aquatic Samples	550
Soil Samples	3047
Animal Samples	3634
Plant Samples	943
Total Samples	8174

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18050]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Rummeliibacillus stabekisii gene for 16S rRNA, partial sequence, strain: NBRC 104870	AB682217	1473	ENA	241244 tax ID	*
[Ref.: #18050]	Rummeliibacillus stabekisii strain KSC-SF6g 16S ribosomal RNA (rrn) gene, partial sequence	DQ870754	1462	ENA	241244 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Rummeliibacillus stabekisii NBRC 104870	GCA_007988965	contig	GenBank	241244 tax ID	*
[Ref.: #66792]	Rummeliibacillus stabekisii DSM 25578	GCA_014202625	scaffold	GenBank	241244 tax ID	*
[Ref.: #66792]	Rummeliibacillus stabekisii strain DSM 25578	241244.14	wgs	PATRIC	241244 tax ID	*
[Ref.: #66792]	Rummeliibacillus stabekisii strain NBRC 104870	241244.13	wgs	PATRIC	241244 tax ID	*
[Ref.: #66792]	Rummeliibacillus stabekisii DSM 25578	2861577009	draft	JGI IMG/M	241244 tax ID	*
[Ref.: #66792]	Staphylococcus aureus E1470	GCA_001045725	contig	PATRIC	1280 tax ID	*

[Ref.: #29095]

GC-content

37.3 mol%

Availability in culture collections External links

[Ref.: #18050]

Culture collection no.

DSM 25578, NBRC 104870, NRRL B-51320

[Ref.: #20218]

Associated Passport(s) in StrainInfo

842288

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Description of Rummeliibacillus stabekisii gen. nov., sp. nov. and reclassification of Bacillus pycnus Nakamura et al. 2002 as Rummeliibacillus pycnus comb. nov.	Vaishampayan P, Miyashita M, Ohnishi A, Satomi M, Rooney A, La Duc MT, Venkateswaran K	Int J Syst Evol Microbiol	10.1099/ijs.0.006098-0	2009	*
Phylogeny	Rummeliibacillus suwonensis sp. nov., isolated from soil collected in a mountain area of South Korea.	Her J, Kim J	J Microbiol	10.1007/s12275-013-3126-5	2013	*

References

#18050

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25578

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#29095

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25524 (see below)

#37908 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#25524

IJSEM 1094 2009 ( DOI 10.1099/ijs.0.006098-0 , PubMed 19406799 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Rummeliibacillus stabekisii in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF