Name: Exiguobacterium aurantiacum BL 77/1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 18105

Type strain:

Species: Exiguobacterium aurantiacum

Strain Designation: BL 77/1

Culture col. no.: DSM 6208, ATCC 35652, NCDO 2321, NCIB 11798, CCUG 44910, NCIMB 11798, IFO 14763, CIP 103353

Strain history: CIP <- 1989, NCIB <- B.M. Lund: strain BL 77/1

NCBI tax ID(s): 1397694 (strain), 33987 (species)

SI-ID 368693

ATCC 35652, NCIB 11798, CIP 103353, DSM 6208, NCDO 2321, NCIMB 11798, IFO14763, KCTC 9244, NCFB 2321, CCUG 44910, NBRC 14763, BCRC 17392, CCRC 17392

Other strains from Exiguobacterium aurantiacum

E. aurantiacum BL 77/4, DSM 6203, NCDO 2323
E. aurantiacum BL 77/2, DSM 6209, NCDO 2322
E. aurantiacum STI09714(IMET), V-60,
E. aurantiacum CIP 102442
E. aurantiacum LRA 143 06 89, CIP 103729, ATCC ...
E. aurantiacum CCUG 56748

Section

Exiguobacterium aurantiacum BL 77/1 is an aerobe, mesophilic, motile bacterium that was isolated from alkaline effluent from potato washing.

motile
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Exiguobacterium*
	Species	**Exiguobacterium aurantiacum**
	Full Scientific Name (LPSN)	Exiguobacterium aurantiacum Collins et al. 1984

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #69480]	Motility	yes Confidence in %93.643

[Ref.: #19722]	Colony color	Honey yellow
[Ref.: #19722]	Incubation period	10-14 days
[Ref.: #19722]	Cultivation medium used	ISP 6

[Ref.: #19722]	Incubation period	10-14 days
[Ref.: #19722]	Cultivation medium used	ISP 2

[Ref.: #19722]	Incubation period	10-14 days
[Ref.: #19722]	Cultivation medium used	ISP 5

[Ref.: #19722]	Multicellular complex forming ability	no
[Ref.: #19722]	Medium Name (multicellularity)	ISP 2

[Ref.: #19722]	Multicellular complex forming ability	no
[Ref.: #19722]	Medium Name (multicellularity)	ISP 5

[Ref.: #19722]	Multicellular complex forming ability	no
[Ref.: #19722]	Medium Name (multicellularity)	ISP 6

Information on culture and growth conditions Culture and growth conditions

[Ref.: #2534]

Culture medium

EXIGUOBACTERIUM MEDIUM (DSMZ Medium 468)

[Ref.: #2534]

Culture medium growth

[Ref.: #2534]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2534]

Culture medium composition

expand / minimize

[Ref.: #19722]

Culture medium

ISP 2

[Ref.: #19722]

Culture medium growth

[Ref.: #19722]

Culture medium composition

expand / minimize

[Ref.: #19722]

Culture medium

ISP 5

[Ref.: #19722]

Culture medium growth

[Ref.: #19722]

Culture medium composition

expand / minimize

[Ref.: #19722]

Culture medium

ISP 6

[Ref.: #19722]

Culture medium growth

[Ref.: #19722]

Culture medium composition

expand / minimize

[Ref.: #40617]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #40617]

Culture medium growth

[Ref.: #40617]

Culture medium composition

expand / minimize

[Ref.: #116557]

Culture medium

CIP Medium 3

[Ref.: #116557]

Culture medium growth

[Ref.: #116557]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #2534]	growth	28 °C
[Ref.: #19722]	optimum	28 °C
[Ref.: #40617]	growth	30 °C
[Ref.: #56539]	growth	25 °C
[Ref.: #56539]	growth	30 °C

[Ref.: #2534]	Temperature range	mesophilic
[Ref.: #19722]	Temperature range	mesophilic
[Ref.: #40617]	Temperature range	mesophilic
[Ref.: #56539]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #56539]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #19722]		NaCl		growth	0	%

[Ref.: #2534]	Murein short key	A11.01
[Ref.: #2534]	Murein types	A3alpha L-Lys-Gly

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #19722]	arabinose ChEBI	+
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #19722]	cellulose ChEBI	-
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #19722]	fructose ChEBI	+
[Ref.: #68368]	gelatin ChEBI	+	hydrolysis	* from API 20E, API Coryne
[Ref.: #19722]	glucose ChEBI	+
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #19722]	mannitol ChEBI	-
[Ref.: #19722]	myo-inositol ChEBI	-
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #19722]	raffinose ChEBI	-
[Ref.: #19722]	rhamnose ChEBI	+/-
[Ref.: #19722]	sucrose ChEBI	+
[Ref.: #68379]	sucrose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E, API Coryne
[Ref.: #19722]	xylose ChEBI	+
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68379]	alpha-glucosidase	-	3.2.1.20	* from API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne, API 20E, API zym
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne, API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	+		* from API Coryne, API 20E
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne, API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #68379]	pyrazinamidase	+	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne, API 20E
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #19722]	504	-	-	-	-	-	-	-	-	-	+	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #19722]	268	-	+	+	-	-	-	-	-	-	-	+	not determinedn.d.	-	-	-	-	-	-	+	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #19722]	547	not determinedn.d.	+	+	+	-	-	-	-	-	-	-	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #2534]	Sample type/isolated from	alkaline effluent from potato washing
[Ref.: #2534]	Country	United Kingdom
[Ref.: #2534]	Country ISO 3 Code	GBR
[Ref.: #2534]	Continent	Europe

[Ref.: #56539]	Sample type/isolated from	Alkaline effluent from potato washing

[Ref.: #116557]	Sample type/isolated from	Other, Effluent of a potato processing factory
* marker position based on {}

Isolation sources categories

#Engineered	#Waste	#Wastewater
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Condition	#Alkaline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence DQ019166 (>99% sequence identity) for Exiguobacterium aurantiacum from Microbeatlas

Aquatic Samples	1593
Soil Samples	374
Animal Samples	2195
Plant Samples	417
Total Samples	4579

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2534]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #19722]	Biosafety level	1	Risk group (German classification)
[Ref.: #116557]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Exiguobacterium aurantiacum strain DSM 6208 16S ribosomal RNA gene, partial sequence	DQ019166	1552	ENA	33987 tax ID	*
[Ref.: #20218]	Exiguobacterium aurantiacum gene for 16S rRNA, partial sequence, strain: NBRC 14763	AB680657	1487	ENA	33987 tax ID	*
[Ref.: #20218]	E.aurantiacum (NCDO 2321) 16S rRNA	X70316	1527	ENA	33987 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Exiguobacterium aurantiacum DSM 6208	GCA_000702585	contig	GenBank	1397694 tax ID	*
[Ref.: #66792]	Exiguobacterium aurantiacum DSM 6208	1397694.4	wgs	PATRIC	1397694 tax ID	*
[Ref.: #66792]	Exiguobacterium aurantiacum DSM 6208	2562617001	draft	JGI IMG/M	1397694 tax ID	*

[Ref.: #2534]

GC-content

53.4 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Exiguobacterium aurantiacum DSM 6208 PATRIC: 1397694.4
[Ref.: #69481]	spore-forming	yes	80	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Exiguobacterium aurantiacum DSM 6208 PATRIC: 1397694.4
[Ref.: #69480]	motile	yes	91.208	no
[Ref.: #69480]	gram-positive	yes	90.635	no
[Ref.: #69480]	anaerobic	no	98.947	no
[Ref.: #69480]	halophile	yes	73.182	yes
[Ref.: #69480]	spore-forming	no	68.632	no
[Ref.: #69480]	glucose-util	yes	88.485	yes
[Ref.: #69480]	thermophile	no	99.331	no
[Ref.: #69480]	aerobic	yes	71.062	no
[Ref.: #69480]	flagellated	yes	69.665	no
[Ref.: #69480]	glucose-ferment	no	81.421	yes

Availability in culture collections External links

[Ref.: #2534]

Culture collection no.

DSM 6208, ATCC 35652, NCDO 2321, NCIB 11798, CCUG 44910, NCIMB 11798, IFO 14763, CIP 103353

[Ref.: #87093]

SI-ID 368693

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Exiguobacterium alkaliphilum sp. nov. isolated from alkaline wastewater drained sludge of a beverage factory.	Mohan Kulshreshtha N, Kumar R, Begum Z, Shivaji S, Kumar A	Int J Syst Evol Microbiol	10.1099/ijs.0.039123-0	2013	*
Phylogeny	Exiguobacterium himgiriensis sp. nov. a novel member of the genus Exiguobacterium, isolated from the Indian Himalayas.	Singh NK, Raichand R, Kaur I, Kaur C, Pareek S, Mayilraj S	Antonie Van Leeuwenhoek	10.1007/s10482-012-9861-5	2012	*
Phylogeny	Exiguobacterium mexicanum sp. nov. and Exiguobacterium artemiae sp. nov., isolated from the brine shrimp Artemia franciscana.	Lopez-Cortes A, Schumann P, Pukall R, Stackebrandt E	Syst Appl Microbiol	10.1016/j.syapm.2005.09.007	2005	*
Phylogeny	Exiguobacterium undae sp. nov. and Exiguobacterium antarcticum sp. nov.	Fruhling A, Schumann P, Hippe H, Straubler B, Stackebrandt E	Int J Syst Evol Microbiol	10.1099/00207713-52-4-1171	2002	*
Enzymology	Molecular characterization of guanosine kinase gene from a facultative alkalophile, Exiguobacterium aurantiacum ATCC 35652.	Usuda Y, Kawasaki H, Shimaoka M, Utagawa T	Biochim Biophys Acta	10.1016/s0167-4781(98)00166-3	1998	*

References

#2534

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 6208

#19722

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40617 ; Curators of the CIP;
#56539 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 44910

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68368

Automatically annotated from API 20E .

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#87093

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID368693.1 )

#116557 Collection of Institut Pasteur ; Curators of the CIP; CIP 103353
* These data were automatically processed and therefore are not curated

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Exiguobacterium aurantiacum DSM 6208

Exiguobacterium aurantiacum DSM 6208

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