Name: Exiguobacterium acetylicum 1005
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 18103

Type strain:

Species: Exiguobacterium acetylicum

Strain Designation: 1005

Culture col. no.: DSM 20416, ATCC 953, NCIB 9889, CCUG 32630, JCM 1968, BCRC 10378, BCRC 17390, CGMCC 1.1917, CIP 82.109, HAMBI 2009, IAM 1790, IFO 12146, IMET 11072, KCTC 3255, NBRC 12146, NCCB 70030, NCIMB 9889

Strain history: CIP <- 1982, ATCC, Brevibacterium acetylicum <- M. Levine: strain 1005

NCBI tax ID(s): 1397697 (strain), 41170 (species)

SI-ID 45045

ATCC 21665, IFO 12146, ATCC 953, NCIB 9889, CCUG 32630, DSM 20416, IAM 1790, IMET 11072, JCM 1968, CCRC 10378, NCIMB 9889, CIP 82.109, KCTC 3255, NBRC 12146, NCCB 70030, LMD 70.30, HAMBI 2009, BCRC 10378, CCT 0517, BCRC 17390, CCRC 17390, CGMCC 1.1917

Other strains from Exiguobacterium acetylicum

E. acetylicum CIP 102738
E. acetylicum CIP 102196
E. acetylicum CIP 102530
E. acetylicum 85.87, CIP 102826
E. acetylicum 61.87, CIP 102814
E. acetylicum 63.87, CIP 102815
E. acetylicum 625.85, CIP 101809
E. acetylicum R 555, CIP 101227
E. acetylicum 482-86, CIP 102463
E. acetylicum 62.87, CIP 102816

Section

Exiguobacterium acetylicum 1005 is a mesophilic bacterium that was isolated from creamery waste.

mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Exiguobacterium*
	Species	**Exiguobacterium acetylicum**
	Full Scientific Name (LPSN)	Exiguobacterium acetylicum (Levine and Soppeland 1926) Farrow et al. 1994

	Synonym	Brevibacterium acetylicum
	Synonym	"Flavobacterium* acetylicum"*

Information on morphological and physiological properties Morphology

[Ref.: #18440]	Colony color	Daffodil yellow (1007)
[Ref.: #18440]	Incubation period	10-14 days
[Ref.: #18440]	Cultivation medium used	ISP 2

[Ref.: #18440]	Colony color	Daffodil yellow (1007)
[Ref.: #18440]	Incubation period	10-14 days
[Ref.: #18440]	Cultivation medium used	ISP 3

[Ref.: #18440]	Colony color	beige
[Ref.: #18440]	Incubation period	10-14 days
[Ref.: #18440]	Cultivation medium used	ISP 4

[Ref.: #18440]	Colony color	beige
[Ref.: #18440]	Incubation period	10-14 days
[Ref.: #18440]	Cultivation medium used	ISP 5

[Ref.: #18440]	Colony color	beige
[Ref.: #18440]	Incubation period	10-14 days
[Ref.: #18440]	Cultivation medium used	ISP 6

[Ref.: #18440]	Colony color	beige
[Ref.: #18440]	Incubation period	10-14 days
[Ref.: #18440]	Cultivation medium used	ISP 7

[Ref.: #18440]	Multicellular complex forming ability	no
[Ref.: #18440]	Medium Name (multicellularity)	ISP 2

[Ref.: #18440]	Multicellular complex forming ability	no
[Ref.: #18440]	Medium Name (multicellularity)	ISP 3

[Ref.: #18440]	Multicellular complex forming ability	no
[Ref.: #18440]	Medium Name (multicellularity)	ISP 4

[Ref.: #18440]	Multicellular complex forming ability	no
[Ref.: #18440]	Medium Name (multicellularity)	ISP 5

[Ref.: #18440]	Multicellular complex forming ability	no
[Ref.: #18440]	Medium Name (multicellularity)	ISP 6

[Ref.: #18440]	Multicellular complex forming ability	no
[Ref.: #18440]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #18440]

Culture medium

ISP 2

[Ref.: #18440]

Culture medium growth

[Ref.: #18440]

Culture medium composition

expand / minimize

[Ref.: #18440]

Culture medium

ISP 3

[Ref.: #18440]

Culture medium growth

[Ref.: #18440]

Culture medium composition

expand / minimize

[Ref.: #18440]

Culture medium

ISP 4

[Ref.: #18440]

Culture medium growth

[Ref.: #18440]

Culture medium composition

expand / minimize

[Ref.: #18440]

Culture medium

ISP 5

[Ref.: #18440]

Culture medium growth

[Ref.: #18440]

Culture medium composition

expand / minimize

[Ref.: #18440]

Culture medium

ISP 6

[Ref.: #18440]

Culture medium growth

[Ref.: #18440]

Culture medium composition

expand / minimize

[Ref.: #18440]

Culture medium

ISP 7

[Ref.: #18440]

Culture medium growth

[Ref.: #18440]

Culture medium composition

expand / minimize

[Ref.: #8801]

Culture medium

CORYNEBACTERIUM AGAR (DSMZ Medium 53)

[Ref.: #8801]

Culture medium growth

[Ref.: #8801]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8801]

Culture medium composition

expand / minimize

[Ref.: #34012]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #34012]

Culture medium growth

[Ref.: #34012]

Culture medium composition

expand / minimize

[Ref.: #115992]

Culture medium

CIP Medium 3

[Ref.: #115992]

Culture medium growth

[Ref.: #115992]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #8801]	growth	30 °C
[Ref.: #18440]	optimum	28 °C
[Ref.: #34012]	growth	30 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #8801]	Temperature range	mesophilic
[Ref.: #18440]	Temperature range	mesophilic
[Ref.: #34012]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #8801]	Murein short key	A11.01
[Ref.: #8801]	Murein types	A3alpha L-Lys-Gly

[Ref.: #67770]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne, API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne, API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68379]	beta-galactosidase	+	3.2.1.23	* from API Coryne, API zym
[Ref.: #68379]	beta-glucosidase	+	3.2.1.21	* from API Coryne, API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne, API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #18440]	117	-	-	-	-	-	+	+	-	+	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #18440]	152	not determinedn.d.	-	+	+	-	-	-	-	-	-	-	-	-	+	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8801]	Sample type/isolated from	creamery waste

[Ref.: #67770]	Sample type/isolated from	Creamy waste

[Ref.: #115992]	Sample type/isolated from	Food, Milk skimmed
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Dairy product
#Engineered	#Industrial	#Industrial production
#Engineered	#Waste	#Industrial waste

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence DQ019167 (>99% sequence identity) for Exiguobacterium from Microbeatlas

Aquatic Samples	18655
Soil Samples	9088
Animal Samples	28214
Plant Samples	7886
Total Samples	63843

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18440]	Biosafety level	1	German classification
[Ref.: #8801]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #115992]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Exiguobacterium acetylicum strain DSM 20416 16S ribosomal RNA gene, partial sequence	DQ019167	1550	ENA	41170 tax ID	*
[Ref.: #20218]	Exiguobacterium acetylicum 16S rRNA gene, partial sequence	D55730	1482	ENA	41170 tax ID	*
[Ref.: #20218]	Exiguobacterium acetylicum gene for 16S rRNA, partial sequence, strain: NBRC 12146	AB680246	1487	ENA	41170 tax ID	*
[Ref.: #20218]	B.acetylicum (NCIMB 9889) 16S rRNA	X70313	1473	ENA	41170 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Exiguobacterium acetylicum DSM 20416	GCA_000702605	contig	GenBank	1397697 tax ID
[Ref.: #66792]	Exiguobacterium acetylicum DSM 20416	1397697.3	wgs	PATRIC	1397697 tax ID	*
[Ref.: #66792]	Exiguobacterium acetylicum DSM 20416	2562617000	draft	JGI IMG/M	1397697 tax ID	*

[Ref.: #8801]	GC-content	46.6 mol%
[Ref.: #67770]	GC-content	46.6 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Exiguobacterium acetylicum DSM 20416 PATRIC: 1397697.3
[Ref.: #69480]	motile	yes	89.261	no
[Ref.: #69480]	gram-positive	yes	86.789	no
[Ref.: #69480]	anaerobic	no	98.467	no
[Ref.: #69480]	halophile	yes	74.89	no
[Ref.: #69480]	spore-forming	no	75.958	no
[Ref.: #69480]	glucose-util	yes	89.628	no
[Ref.: #69480]	thermophile	no	99.527	no
[Ref.: #69480]	aerobic	yes	89.657	no
[Ref.: #69480]	flagellated	yes	72.524	no
[Ref.: #69480]	glucose-ferment	no	77.802	yes

Availability in culture collections External links

[Ref.: #8801]

Culture collection no.

DSM 20416, ATCC 953, NCIB 9889, CCUG 32630, JCM 1968, BCRC 10378, BCRC 17390, CGMCC 1.1917, CIP 82.109, HAMBI 2009, IAM 1790, IFO 12146, IMET 11072, KCTC 3255, NBRC 12146, NCCB 70030, NCIMB 9889

[Ref.: #87091]

SI-ID 45045

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Development of an improved assay for purine nucleoside kinase activity in cell extracts and detection of inosine kinase activity in Brevibacterium acetylicum ATCC 953, related species, and Corynebacterium flaccumfaciens ATCC 6887.	Kawasaki H, Usuda Y, Shimaoka M, Utagawa T	Biosci Biotechnol Biochem	10.1271/bbb.64.761	2000	*
Phylogeny	Exiguobacterium enclense sp. nov., isolated from sediment.	Dastager SG, Mawlankar R, Sonalkar VV, Thorat MN, Mual P, Verma A, Krishnamurthi S, Tang SK, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.000149	2015	*
Phylogeny	Exiguobacterium soli sp. nov., a psychrophilic bacterium from the McMurdo Dry Valleys, Antarctica.	Chaturvedi P, Prabahar V, Manorama R, Pindi PK, Bhadra B, Begum Z, Shivaji S	Int J Syst Evol Microbiol	10.1099/ijs.0.2008/000067-0	2008	*
Phylogeny	Exiguobacterium indicum sp. nov., a psychrophilic bacterium from the Hamta glacier of the Himalayan mountain ranges of India.	Chaturvedi P, Shivaji S	Int J Syst Evol Microbiol	10.1099/ijs.0.64508-0	2006	*
Phylogeny	Exiguobacterium undae sp. nov. and Exiguobacterium antarcticum sp. nov.	Fruhling A, Schumann P, Hippe H, Straubler B, Stackebrandt E	Int J Syst Evol Microbiol	10.1099/00207713-52-4-1171	2002	*
Enzymology	Molecular cloning and transcriptional analysis of a guanosine kinase gene of Brevibacterium acetylicum ATCC 953.	Usuda Y, Kawasaki H, Shimaoka M, Utagawa T	J Bacteriol	10.1128/jb.179.22.6959-6964.1997	1997	*
Enzymology	Characterization of guanosine kinase from Brevibacterium acetylicum ATCC 953.	Usuda Y, Kawasaki H, Shimaoka M, Utagawa T	Biochim Biophys Acta	10.1016/s0167-4838(97)00080-0	1997	*

References

#8801

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20416

#18440

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#34012 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#87091

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID45045.1 )

#115992 Collection of Institut Pasteur ; Curators of the CIP; CIP 82.109
* These data were automatically processed and therefore are not curated

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Exiguobacterium acetylicum DSM 20416

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