Name: Murdochiella asaccharolytica DSM 23919
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 18084

Type strain:

Species: Murdochiella asaccharolytica

Culture col. no.: DSM 23919, ATCC BAA-1631, CCUG 55976, WAL 1855C

Strain history: <- CCUG <- S. M. Finegold, Dept. Veteran Affairs Medical Center, Los Angeles, USA; WAL 1855C {2007}

NCBI tax ID(s): 507844 (species)

SI-ID 402162

DSM 23919, CCUG 55976

Section

Murdochiella asaccharolytica DSM 23919 is an anaerobe, mesophilic, Gram-positive human pathogen that was isolated from sacral pilonidal cyst aspirate from an immunocompetent patient.

coccus-shaped
Gram-positive
anaerobe
human pathogen
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Peptoniphilaceae*
	Genus	*Murdochiella*
	Species	**Murdochiella asaccharolytica**
	Full Scientific Name (LPSN)	Murdochiella asaccharolytica Ulger-Toprak et al. 2010

Information on morphological and physiological properties Morphology

[Ref.: #29561]	Gram stain	positive

[Ref.: #29561]	Cell width	0.55 µm

[Ref.: #29561]	Cell shape	coccus-shaped

[Ref.: #29561]	Motility	no

[Ref.: #17425]	Type of hemolysis	gamma

[Ref.: #60812]	Incubation period	4 days

[Ref.: #29561]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #17425]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #17425]

Culture medium growth

[Ref.: #17425]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17425]

Culture medium composition

expand / minimize

[Ref.: #17425]

Culture medium

CHOPPED MEAT MEDIUM (DSMZ Medium 78)

[Ref.: #17425]

Culture medium growth

[Ref.: #17425]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17425]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #17425]			growth	37 °C
[Ref.: #60812]			growth	37 °C

[Ref.: #17425]	Temperature range	mesophilic
[Ref.: #60812]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #17425]	Oxygen tolerance	anaerobe
[Ref.: #29561]	Oxygen tolerance	anaerobe
[Ref.: #60812]	Oxygen tolerance	anaerobe

[Ref.: #29561]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	+	hydrolysis	* from API rID32A
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A

	Metabolite production	Metabolite	Production
[Ref.: #29561]		indole ChEBI	yes

	Physiological tests	Metabolite	Indole test
[Ref.: #29561]		indole ChEBI	+

	Enzyme	Enzyme activity	EC number
[Ref.: #68380]	alanine arylamidase	+	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	-	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #68380]	alpha-galactosidase	-	3.2.1.22	* from API rID32A
[Ref.: #68380]	alpha-glucosidase	-	3.2.1.20	* from API rID32A
[Ref.: #68380]	arginine dihydrolase	+	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-galactosidase	-	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	-	3.2.1.21	* from API rID32A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #17425]	catalase	-	1.11.1.6
[Ref.: #17425]	cytochrome-c oxidase	-	1.9.3.1
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	glycin arylamidase	+		* from API rID32A
[Ref.: #68380]	histidine arylamidase	+		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	+		* from API rID32A
[Ref.: #68380]	leucine arylamidase	+	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	+	3.4.11.1	* from API rID32A
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32A
[Ref.: #68380]	phenylalanine arylamidase	+		* from API rID32A
[Ref.: #68380]	proline-arylamidase	+	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	+		* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #17425]	12442	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	-	+	+	+	+	+	-	+/-	+	+	+	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #17425]	Sample type/isolated from	sacral pilonidal cyst aspirate from an immunocompetent patient
[Ref.: #17425]	Geographic location (country and/or sea, region)	California, Los Angeles
[Ref.: #17425]	Country	USA
[Ref.: #17425]	Country ISO 3 Code	USA
[Ref.: #17425]	Continent	North America

[Ref.: #60812]	Sample type/isolated from	Human sacrum pilonidal cyst
[Ref.: #60812]	Sampling date	2007-02-14
[Ref.: #60812]	Geographic location (country and/or sea, region)	CA,Los Angeles
[Ref.: #60812]	Country	USA
[Ref.: #60812]	Country ISO 3 Code	USA
[Ref.: #60812]	Continent	North America
* marker position based on {}

Isolation sources categories

#Infection	#Patient	-
#Host Body-Site	#Organ	#Skin, Nail, Hair
#Host Body Product	#Fluids	#Aspirate

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EU483153 (>99% sequence identity) for Levyella massiliensis from Microbeatlas

Aquatic Samples	739
Soil Samples	854
Animal Samples	39549
Plant Samples	171
Total Samples	41313

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #17425]	Pathogenicity (human)	yes, in single cases
[Ref.: #17425]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #17425]	Murdochiella asaccharolytica strain WAL 1855C 16S ribosomal RNA gene, partial sequence	EU483153	1362	ENA	507844 tax ID

Availability in culture collections External links

[Ref.: #17425]

Culture collection no.

DSM 23919, ATCC BAA-1631, CCUG 55976, WAL 1855C

[Ref.: #87072]

SI-ID 402162

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Murdochiella asaccharolytica gen. nov., sp. nov., a Gram-stain-positive, anaerobic coccus isolated from human wound specimens.	Ulger-Toprak N, Liu C, Summanen PH, Finegold SM	Int J Syst Evol Microbiol	10.1099/ijs.0.015909-0	2009	*

References

#17425

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23919

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29561

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25956 (see below)

#60812 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 55976

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#87072

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID402162.1 )

#25956

IJSEM 1013 2010 ( DOI 10.1099/ijs.0.015909-0 , PubMed 19666803 )

* These data were automatically processed and therefore are not curated

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