Proteocatella sphenisci PPP2 | Type strain | DSM 23131, CIP 108034, JCM 12175, ATCC BAA-755 | BacDiveID:18080

Strain identifier

BacDive ID: 18080

Type strain:

Species: Proteocatella sphenisci

Strain Designation: PPP2

Culture col. no.: DSM 23131, CIP 108034, JCM 12175, ATCC BAA-755

Strain history: E. V. Pikuta PPP2.

NCBI tax ID(s): 1123009 (strain), 181070 (species)

SI-ID 403712

DSM 23131

Section

Proteocatella sphenisci PPP2 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from guano of Magellanic penguins Spheniscus magellanicus.

Gram-positive
motile
rod-shaped
spore-forming
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Peptostreptococcaceae*
	Genus	*Proteocatella*
	Species	**Proteocatella sphenisci**
	Full Scientific Name (LPSN)	Proteocatella sphenisci Pikuta et al. 2009

Information on morphological and physiological properties Morphology

[Ref.: #28967]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %99.72

[Ref.: #28967]	Cell length	4 µm

[Ref.: #28967]	Cell width	0.75 µm

[Ref.: #28967]	Cell shape	rod-shaped

[Ref.: #28967]	Motility	yes

Multimedia content

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Information on culture and growth conditions Culture and growth conditions

Culture medium

ACETOMICROBIUM FAECALIS MEDIUM (DSMZ Medium 412)

Culture medium growth

Culture medium link

Medium recipe at MediaDive

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #16721]			growth	30 °C
[Ref.: #28967]			growth	02-37 °C
[Ref.: #28967]			optimum	29 °C
[Ref.: #67770]			growth	30 °C

[Ref.: #16721]	Temperature range	mesophilic
[Ref.: #28967]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #28967]			optimum	8.3
[Ref.: #28967]			growth	6.7-9.7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #28967]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.005

[Ref.: #28967]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %96
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.846

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #28967]		NaCl		growth	0-4	%
[Ref.: #28967]		NaCl		optimum	0.5	%

Observation

aggregates in chains

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]		arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #68380]		D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]		L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #68380]		nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #68380]		raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]		tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]		urea ChEBI	-	hydrolysis	* from API rID32A

	Metabolite production	Metabolite	Production
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #68380]		alanine arylamidase	-	3.4.11.2	* from API rID32A
[Ref.: #68380]		alkaline phosphatase	-	3.1.3.1	* from API rID32A
[Ref.: #68380]		alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68380]		alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #68380]		alpha-galactosidase	-	3.2.1.22	* from API rID32A
[Ref.: #68380]		alpha-glucosidase	-	3.2.1.20	* from API rID32A
[Ref.: #68380]		arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68380]		beta-galactosidase	-	3.2.1.23	* from API rID32A
[Ref.: #68380]		beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]		beta-glucosidase	-	3.2.1.21	* from API rID32A
[Ref.: #68380]		beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #68380]		glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]		glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]		glycin arylamidase	-		* from API rID32A
[Ref.: #68380]		histidine arylamidase	-		* from API rID32A
[Ref.: #68380]		L-arginine arylamidase	-		* from API rID32A
[Ref.: #68380]		leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]		leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]		N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32A
[Ref.: #68380]		phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]		pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]		serine arylamidase	-		* from API rID32A
[Ref.: #68380]		tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]		tyrosine arylamidase	-		* from API rID32A
[Ref.: #68380]		urease	-	3.5.1.5	* from API rID32A
		Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #16721]	12775	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16721]	Sample type/isolated from	guano of Magellanic penguins Spheniscus magellanicus
[Ref.: #16721]	Host species	Spheniscus magellanicus
[Ref.: #16721]	Geographic location (country and/or sea, region)	coast of Chilean Patagonia
[Ref.: #16721]	Country	Chile
[Ref.: #16721]	Country ISO 3 Code	CHL
[Ref.: #16721]	Continent	Middle and South America

[Ref.: #67770]	Sample type/isolated from	Guano of the Magellanic penguin in Chilean Patagonia
* marker position based on {}

Isolation sources categories

#Host	#Birds	-
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

What are isolation sources categories?

Global distribution of 16S sequence AF450134 (>99% sequence identity) for Proteocatella sphenisci subclade from Microbeatlas

Aquatic Samples	1029
Soil Samples	37
Animal Samples	59
Plant Samples	9
Total Samples	1134

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

Biosafety level

1

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16721]	Proteocatella sphenisci strain PPP2 16S ribosomal RNA gene, partial sequence	AF450134	1450	ENA	181070 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Proteocatella sphenisci DSM 23131	GCA_000423525	scaffold	GenBank	1123009 tax ID
[Ref.: #66792]	Proteocatella sphenisci DSM 23131	1123009.3	wgs	PATRIC	1123009 tax ID	*
[Ref.: #66792]	Proteocatella sphenisci DSM 23131	2523533562	draft	JGI IMG/M	1123009 tax ID	*

[Ref.: #16721]	GC-content	39.5 mol%	high performance liquid chromatography (HPLC)
[Ref.: #28967]	GC-content	39.5 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Proteocatella sphenisci DSM 23131 DSM 23131 NCBI: GCA_000423525.1
[Ref.: #69481]	spore-forming	yes	96	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Proteocatella sphenisci DSM 23131 PATRIC: 1123009.3
[Ref.: #69480]	motile	yes	89.185	yes
[Ref.: #69480]	flagellated	no	67.68	no
[Ref.: #69480]	gram-positive	yes	84.954	yes
[Ref.: #69480]	anaerobic	yes	99.611	no
[Ref.: #69480]	aerobic	no	96.68	yes
[Ref.: #69480]	halophile	no	90.81	yes
[Ref.: #69480]	spore-forming	yes	69.708	yes
[Ref.: #69480]	glucose-util	yes	87.296	no
[Ref.: #69480]	thermophile	no	83.801	yes
[Ref.: #69480]	glucose-ferment	yes	56.815	no

Availability in culture collections External links

Culture collection no.

DSM 23131, CIP 108034, JCM 12175, ATCC BAA-755

SI-ID 403712

*

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Proteocatella sphenisci gen. nov., sp. nov., a psychrotolerant, spore-forming anaerobe isolated from penguin guano.	Pikuta EV, Hoover RB, Marsic D, Whitman WB, Lupa B, Tang J, Krader P	Int J Syst Evol Microbiol	10.1099/ijs.0.002816-0	2009	*

References

#16721

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23131

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#28967

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25404 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66793

Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#87068

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403712.1 )

#25404

IJSEM 2302 2009 ( DOI 10.1099/ijs.0.002816-0 , PubMed 19620379 )

* These data were automatically processed and therefore are not curated

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