Thermoanaerobacterium saccharolyticum (DSM 7060, ATCC 49915, B6A-RI)

Name: Thermoanaerobacterium saccharolyticum (DSM 7060, ATCC 49915, B6A-RI)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 18052

Type strain

Strain Designation B6A-RI

Culture col. no. DSM 7060 ATCC 49915 B6A-RI

NCBI tax ID(s) 1123370 28896

Special Collections DSMZ

History DSMZ <- J. G. Zeikus, Michigan State University, Department of Biochemistry, East Lansing, MI 48824, USA; B6A-RI <- C. Y. Lee

Links

Thermoanaerobacterium saccharolyticum B6A-RI is an anaerobe bacterium that was isolated from thermal spring.

anaerobe 16S sequence Bacteria

Name and taxonomic classification

SI-ID 42752

ATCC 49915,DSM 7060,BCRC 17463,CCRC 17463

@ref 20215

Last LPSN update 2026-05-29 08:29:09

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Thermoanaerobacterales

Family Thermoanaerobacteraceae

Genus Thermoanaerobacterium

Species Thermoanaerobacterium saccharolyticum

Full scientific name Thermoanaerobacterium saccharolyticum Lee et al. 1993

BacDive ID	Other strains from **Thermoanaerobacterium saccharolyticum** (1)	Type strain
18053	T. saccharolyticum JW/SL-YS 485, DSM 8691

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
2966	THERMOANAEROBACTER BROCKII MEDIUM (DSMZ Medium 144)		Medium recipe at MediaDive	Name: THERMOANAEROBACTER BROCKII MEDIUM (DSMZ Medium 144) Composition: Trypticase peptone 10.0 g/l D-Glucose 5.0 g/l Yeast extract 3.0 g/l K2HPO4 1.5 g/l Na2S x 9 H2O 1.0 g/l NH4Cl 0.9 g/l KH2PO4 0.75 g/l MgCl2 x 6 H2O 0.4 g/l Nitrilotriacetic acid 0.1152 g/l NaCl 0.009 g/l FeSO4 x 7 H2O 0.003 g/l FeCl2 x 4 H2O 0.0018 g/l CoCl2 x 6 H2O 0.00153 g/l MnCl2 x 4 H2O 0.0009 g/l CaCl2 x 2 H2O 0.0009 g/l ZnCl2 0.0009 g/l Sodium resazurin 0.0005 g/l NiCl2 x 6 H2O 0.00027 g/l Na2SeO3 x 5 H2O 0.00027 g/l CuCl2 0.00018 g/l H3BO3 9e-05 g/l Na2MoO4 x 2 H2O 9e-05 g/l Pyridoxine hydrochloride 5e-05 g/l p-Aminobenzoic acid 2.5e-05 g/l (DL)-alpha-Lipoic acid 2.5e-05 g/l Riboflavin 2.5e-05 g/l Calcium D-(+)-pantothenate 2.5e-05 g/l Thiamine HCl 2.5e-05 g/l Nicotinic acid 2.5e-05 g/l Biotin 1e-05 g/l Folic acid 1e-05 g/l Vitamin B12 5e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
2966	positive	growth	60

Physiology and metabolism

Oxygen tolerance

2966

Oxygen toleranceanaerobe

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
2966	thermal spring	Wyoming, Yellowstone Natl. Park	USA	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence L09169 (>99% sequence identity) for Thermoanaerobacterium saccharolyticum from Microbeatlas

Aquatic Samples	23
Soil Samples	7
Animal Samples	275
Plant Samples	4
Total Samples	309

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
2966	1	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
2966	Thermoanaerobacterium saccharolyticum (DSM 7060) 16S ribosomal RNA (16S rRNA) gene	L09169	1552		1123370

GC content

2966

GC-content (mol%)36.0

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Characterization of xylan utilization and discovery of a new endoxylanase in Thermoanaerobacterium saccharolyticum through targeted gene deletions.	Podkaminer KK, Guss AM, Trajano HL, Hogsett DA, Lynd LR.	Appl Environ Microbiol	10.1128/aem.02130-12	2012
	Natural competence in Thermoanaerobacter and Thermoanaerobacterium species.	Shaw AJ, Hogsett DA, Lynd LR.	Appl Environ Microbiol	10.1128/aem.00402-10	2010
Metabolism	Characterizing a Halo-Tolerant GH10 Xylanase from Roseithermus sacchariphilus Strain RA and Its CBM-Truncated Variant.	Teo SC, Liew KJ, Shamsir MS, Chong CS, Bruce NC, Chan KG, Goh KM.	Int J Mol Sci	10.3390/ijms20092284	2019
Enzymology	Characterization of a novel beta-xylosidase, XylC, from Thermoanaerobacterium saccharolyticum JW/SL-YS485.	Shao W, Xue Y, Wu A, Kataeva I, Pei J, Wu H, Wiegel J.	Appl Environ Microbiol	10.1128/aem.01511-10	2011
	The extracellular endo-beta-1,4-xylanase with multidomain from the extreme thermophile Caldicellulosiruptor lactoaceticus is specific for insoluble xylan degradation.	Jia X, Han Y.	Biotechnol Biofuels	10.1186/s13068-019-1480-1	2019
	Fermentation of Mannitol Extracts From Brown Macro Algae by Thermophilic Clostridia.	Chades T, Scully SM, Ingvadottir EM, Orlygsson J.	Front Microbiol	10.3389/fmicb.2018.01931	2018
Phylogeny	Phylogenetic analysis of anaerobic thermophilic bacteria: aid for their reclassification.	Rainey FA, Ward NL, Morgan HW, Toalster R, Stackebrandt E.	J Bacteriol	10.1128/jb.175.15.4772-4779.1993	1993
Enzymology	Molecular cloning, sequencing, and expression of a novel multidomain mannanase gene from Thermoanaerobacterium polysaccharolyticum.	Cann IK, Kocherginskaya S, King MR, White BA, Mackie RI.	J Bacteriol	10.1128/jb.181.5.1643-1651.1999	1999
Metabolism	The glucuronic acid utilization gene cluster from Bacillus stearothermophilus T-6.	Shulami S, Gat O, Sonenshein AL, Shoham Y.	J Bacteriol	10.1128/jb.181.12.3695-3704.1999	1999
Metabolism	Role of alcohols in growth, lipid composition, and membrane fluidity of yeasts, bacteria, and archaea.	Huffer S, Clark ME, Ning JC, Blanch HW, Clark DS.	Appl Environ Microbiol	10.1128/aem.00694-11	2011
Genetics	Stereochemical course and reaction products of the action of beta-xylosidase from Thermoanaerobacterium saccharolyticum strain B6A-RI.	Armand S, Vieille C, Gey C, Heyraud A, Zeikus JG, Henrissat B	Eur J Biochem	10.1111/j.1432-1033.1996.00706.x	1996
Enzymology	Crystal structure of beta-D-xylosidase from Thermoanaerobacterium saccharolyticum, a family 39 glycoside hydrolase.	Yang JK, Yoon HJ, Ahn HJ, Lee BI, Pedelacq JD, Liong EC, Berendzen J, Laivenieks M, Vieille C, Zeikus GJ, Vocadlo DJ, Withers SG, Suh SW	J Mol Biol	10.1016/j.jmb.2003.10.026	2004
Enzymology	Purification and cloning of a thermostable xylose (glucose) isomerase with an acidic pH optimum from Thermoanaerobacterium strain JW/SL-YS 489.	Liu SY, Wiegel J, Gherardini FC	J Bacteriol	10.1128/jb.178.20.5938-5945.1996	1996
Enzymology	Cloning and sequencing of the Thermoanaerobacterium saccharolyticum B6A-RI apu gene and purification and characterization of the amylopullulanase from Escherichia coli.	Ramesh MV, Podkovyrov SM, Lowe SE, Zeikus JG	Appl Environ Microbiol	10.1128/aem.60.1.94-101.1994	1994
Enzymology	Characterization of the active site and thermostability regions of endoxylanase from Thermoanaerobacterium saccharolyticum B6A-RI.	Lee YE, Lowe SE, Henrissat B, Zeikus JG	J Bacteriol	10.1128/jb.175.18.5890-5898.1993	1993
Enzymology	Gene cloning, sequencing, and biochemical characterization of endoxylanase from Thermoanaerobacterium saccharolyticum B6A-RI.	Lee YE, Lowe SE, Zeikus JG	Appl Environ Microbiol	10.1128/aem.59.9.3134-3137.1993	1993
Enzymology	Genetic organization, sequence and biochemical characterization of recombinant beta-xylosidase from Thermoanaerobacterium saccharolyticum strain B6A-RI.	Lee YE, Zeikus JG	J Gen Microbiol	10.1099/00221287-139-6-1235	1993
Enzymology	Cloning, sequencing and biochemical characterization of xylose isomerase from Thermoanaerobacterium saccharolyticum strain B6A-RI.	Lee YE, Ramesh MV, Zeikus JG	J Gen Microbiol	10.1099/00221287-139-6-1227	1993
Enzymology	Regulation and Characterization of Xylanolytic Enzymes of Thermoanaerobacterium saccharolyticum B6A-RI.	Lee YE, Lowe SE, Zeikus JG	Appl Environ Microbiol	10.1128/aem.59.3.763-771.1993	1993
Phylogeny	Themoanaerobacterium calidifontis sp. nov., a novel anaerobic, thermophilic, ethanol-producing bacterium from hot springs in China.	Shang SM, Qian L, Zhang X, Li KZ, Chagan I.	Arch Microbiol	10.1007/s00203-013-0895-5	2013

References

#2966	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7060
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Thermoanaerobacterium saccharolyticum (DSM 7060, ATCC 49915, B6A-RI)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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