Sulfobacillus thermotolerans (DSM 17362, VKM B-2339, Kr1)

Name: Sulfobacillus thermotolerans (DSM 17362, VKM B-2339, Kr1)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 18037

Type strain

Strain Designation Kr1

Culture col. no. DSM 17362 VKM B-2339 Kr1

NCBI tax ID(s) 338644

Special Collections DSMZ Literature strains

Links

Sulfobacillus thermotolerans Kr1 is an aerobe, spore-forming, thermophilic prokaryote that was isolated from sulphide gold-containing concentrat.

spore-forming Gram-positive rod-shaped aerobe thermophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 291013

DSM 17362,VKM B-2339

@ref 20215

Last LPSN update 2025-12-16 09:51:14

Domain Bacillati

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family "Sulfobacillaceae"

Genus Sulfobacillus

Species Sulfobacillus thermotolerans

Full scientific name Sulfobacillus thermotolerans Bogdanova et al. 2006

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
31715	positive	1.5-4.5 µm	0.8-1.2 µm	rod-shaped

Pigmentation

31715

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
6832	SULFOBACILLUS MEDIUM (DSMZ Medium 665)		Medium recipe at MediaDive	Name: SULFOBACILLUS MEDIUM (DSMZ Medium 665) Composition: FeSO4 x 7 H2O 43.2909 g/l (NH4)2SO4 2.93829 g/l MgSO4 x 7 H2O 0.489716 g/l K2HPO4 0.489716 g/l KCl 0.0979431 g/l Ca(NO3)2 0.00979431 g/l Yeast extract 0.00391773 g/l H2SO4 Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
6832	positive	growth	40	thermophilic
31715	positive	growth	20-60
31715	positive	optimum	40	thermophilic

Culture pH

@ref	Ability	Type	PH	PH range
31715	positive	optimum	2
31715	positive	growth	1.2-2.4	acidophile

Physiology and metabolism

Oxygen tolerance

31715

Oxygen toleranceaerobe

Spore formation

31715

Spore formationyes

Observation

31715

Observationaggregates in chains

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
31715	28757 ChEBI	fructose	+	carbon source
31715	17234 ChEBI	glucose	+	carbon source
31715	25115 ChEBI	malate	+	carbon source
31715	17992 ChEBI	sucrose	+	carbon source

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Condition	#Sulfuric

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
6832	sulphide gold-containing concentrat	Eastern Siberia	Russia	RUS	Asia

Taxonmaps

69479

Global distribution of 16S sequence DQ124681 (>99% sequence identity) for Sulfobacillus thermotolerans subclade from Microbeatlas

Aquatic Samples	72
Soil Samples	15
Animal Samples	2
Plant Samples
Total Samples	89

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6832	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM295181v1 assembly for Sulfobacillus thermotolerans Kr1	complete	GCA_002951815	338644.7	8065761495	338644	65.29

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
6832	Sulfobacillus thermotolerans strain Kr1 16S ribosomal RNA gene, partial sequence	DQ124681	1433		338644

GC content

6832

GC-content (mol%)48.2

Genome-based predictions

Predictions

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Sulfobacillus thermotolerans Kr1
NCBI: GCA_002951815.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	71.70	yes
125438	anaerobic	anaerobicⓘ	no	72.17	no
125438	aerobic	aerobicⓘ	yes	67.53	yes
125438	spore-forming	spore-formingⓘ	yes	72.78	no
125438	thermophilic	thermophileⓘ	no	51.35	no
125438	flagellated	motile2+ⓘ	yes	64.93	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Bioleaching of sludge from acid-leached waste traction batteries used in electric vehicles for the extraction of Ni and Co using optimized microbial consortia.	Lu H, Zhang H, Chen X, Wang L, Yan X.	RSC Adv	10.1039/d5ra07472j	2025
	Mechanisms of microbial hyper-resistance to heavy metals: Cellular metal accumulation, metabolic reorganization, and GroEL chaperonin in extremophilic bacterium Sulfobacillus thermotolerans in response to zinc.	Panyushkina A, Matyushkina D, Pobeguts O, Muravyov M, Letarov A.	J Hazard Mater	10.1016/j.jhazmat.2025.137490	2025
Transcriptome	Transcriptomic Analysis of the Strain Acidiplasma sp. YE-1 During the Oxidation of Sulfide Minerals Pyrite and Arsenopyrite.	Bulaev A, Kadnikov V, Elkina Y, Beletsky A, Artykova A, Kolosoff A, Ravin N, Mardanov A.	Int J Mol Sci	10.3390/ijms26199287	2025
Metabolism	Enzymatic Synthesis of l-threo-beta-Hydroxy-alpha-Amino Acids via Asymmetric Hydroxylation Using 2-Oxoglutarate-Dependent Hydroxylase from Sulfobacillus thermotolerans Strain Y0017.	Hara R, Nakajima Y, Yanagawa H, Gawasawa R, Hirasawa I, Kino K.	Appl Environ Microbiol	10.1128/aem.01335-21	2021
	Two-step biohydrometallurgical technology of copper-zinc concentrate processing as an opportunity to reduce negative impacts on the environment.	Fomchenko NV, Muravyov MI.	J Environ Manage	10.1016/j.jenvman.2018.08.045	2018
Metabolism	Sulfobacillus thermotolerans: new insights into resistance and metabolic capacities of acidophilic chemolithotrophs.	Panyushkina AE, Babenko VV, Nikitina AS, Selezneva OV, Tsaplina IA, Letarova MA, Kostryukova ES, Letarov AV.	Sci Rep	10.1038/s41598-019-51486-1	2019
	Understanding Stress Response to High-Arsenic Gold-Bearing Sulfide Concentrate in Extremely Metal-Resistant Acidophile Sulfobacillus thermotolerans.	Panyushkina A, Matyushkina D, Pobeguts O.	Microorganisms	10.3390/microorganisms8071076	2020
	Meta-Analysis of Microbial Communities in Hot Springs: Recurrent Taxa and Complex Shaping Factors beyond pH and Temperature.	Massello FL, Chan CS, Chan KG, Goh KM, Donati E, Urbieta MS.	Microorganisms	10.3390/microorganisms8060906	2020
Metabolism	[Thermoacidophilic micirobial community oxidizing the gold-bearing flotation concentrate of a pyrite-arsenopyrite ore].	Paniushkina AE, Tsaplina IA, Grigor'eva NV, Kondrat'eva TF.	Mikrobiologiia	10.1134/s0026261714040146	2014
	[Phenotypic properties of Sulfobacillus thermotolerans: comparative aspects].	Tsaplina IA, Krasil'nikova EN, Zhuravleva AE, Egorova MA, Zakharchuk LM, Suzina NE, Duda VI, Bogdanova TI, Stadnichuk IN, Kondrat'eva TF.	Mikrobiologiia	10.1134/s0026261708060027	2008
Metabolism	[The dependence of intracellular ATP level on the nutrition mode of the acidophilic bacteria Sulfobacillus thermotolerans and Alicyclobacillus tolerans].	saplina IA, Zhuravleva AE, Ismailov AD, Zakharchuk LM, Krasil'nikova EN, Bogdanova TI, Karavaiko GI.	Mikrobiologiia	10.1134/s0026261707060021	2007
	Distinct Roles of Acidophiles in Complete Oxidation of High-Sulfur Ferric Leach Product of Zinc Sulfide Concentrate.	Muravyov M, Panyushkina A.	Microorganisms	10.3390/microorganisms8030386	2020
Metabolism	[Bacterial diversity analysis of moderately thermophilic microflora enriched by different energy sources].	Liu FF, Zhou HB, Fu B, Qiu GZ.	Wei Sheng Wu Xue Bao		2007
	Culturable and molecular phylogenetic diversity of microorganisms in an open-dumped, extremely acidic Pb/Zn mine tailings.	Tan GL, Shu WS, Hallberg KB, Li F, Lan CY, Zhou WH, Huang LN.	Extremophiles	10.1007/s00792-008-0171-9	2008
	Characterization and genomic analysis of two novel psychrotolerant Acidithiobacillus ferrooxidans strains from polar and subpolar environments.	Munoz-Villagran C, Grossolli-Galvez J, Acevedo-Arbunic J, Valenzuela X, Ferrer A, Diez B, Levican G.	Front Microbiol	10.3389/fmicb.2022.960324	2022
Enzymology	Two large, related, cryptic plasmids from geographically distinct isolates of Sulfobacillus thermotolerans.	Deane SM, Rawlings DE.	Appl Environ Microbiol	10.1128/aem.06118-11	2011
	Column Bioleaching of Fluoride-Containing Secondary Copper Sulfide Ores: Experiments With Sulfobacillus thermosulfidooxidans.	Rodrigues MLM, Santos GHA, Leoncio HC, Leao VA.	Front Bioeng Biotechnol	10.3389/fbioe.2018.00183	2018
Genetics	Lignite coal burning seam in the remote Altai Mountains harbors a hydrogen-driven thermophilic microbial community.	Kadnikov VV, Mardanov AV, Ivasenko DA, Antsiferov DV, Beletsky AV, Karnachuk OV, Ravin NV.	Sci Rep	10.1038/s41598-018-25146-9	2018
Genetics	Comparison of environmental and isolate Sulfobacillus genomes reveals diverse carbon, sulfur, nitrogen, and hydrogen metabolisms.	Justice NB, Norman A, Brown CT, Singh A, Thomas BC, Banfield JF.	BMC Genomics	10.1186/1471-2164-15-1107	2014
Phylogeny	Sulfobacillus harzensis sp. nov., an acidophilic bacterium inhabiting mine tailings from a polymetallic mine.	Zhang R, Hedrich S, Jin D, Breuker A, Schippers A	Int J Syst Evol Microbiol	10.1099/ijsem.0.004871	2021
Phylogeny	Sulfobacillus thermotolerans sp. nov., a thermotolerant, chemolithotrophic bacterium.	Bogdanova TI, Tsaplina IA, Kondrat'eva TF, Duda VI, Suzina NE, Melamud VS, Tourova TP, Karavaiko GI	Int J Syst Evol Microbiol	10.1099/ijs.0.64106-0	2006

References

#6832	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17362
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#27991	IJSEM 1039 2006 ( DOI 10.1099/ijs.0.64106-0 , PubMed 16627651 )
#31715	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27991
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Sulfobacillus thermotolerans (DSM 17362, VKM B-2339, Kr1)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Observation

Metabolite utilization

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002951815

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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