Anaerococcus tetradius (DSM 2951, ATCC 35098, CCM 3634)

Name: Anaerococcus tetradius (DSM 2951, ATCC 35098, CCM 3634)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 17969

Type strain

Culture col. no. DSM 2951 ATCC 35098 CCM 3634 JCM 1964 GIFU 7672 3 more

NCBI tax ID(s) 525255 33036

Special Collections DSMZ CCUG JCM CIP

History DSMZ <- ATCC <- T. Ezaki, GIFU 7672 JCM <-- T. Ezaki GIFU 7672. CIP <- 1993, ATCC <- T. Ezaki: strain GIFU 7672

Links

Anaerococcus tetradius DSM 2951 is an anaerobe, Gram-positive, coccus-shaped bacterium that was isolated from human vagina.

Gram-positive coccus-shaped anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 7938

LMG 14264,ATCC 35098,CCM 3634,DSM 2951,JCM 1964,CIP 103927,CCUG 17637,CCUG 46590,CCUG 46592

@ref 20215

Last LPSN update 2026-05-29 08:29:08

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Tissierellales

Family Peptoniphilaceae

Genus Anaerococcus

Species Anaerococcus tetradius

Full scientific name Anaerococcus tetradius (Ezaki et al. 1983 ex Choukévitch 1911) Ezaki et al. 2001

Synonyms (1)

Peptostreptococcus tetradius

BacDive ID	Other strains from **Anaerococcus tetradius** (8)	Type strain
17966	A. tetradius 01084, DSM 20358
151384	A. tetradius CCUG 44020 A
151522	A. tetradius CCUG 44198
151552	A. tetradius CCUG 44289
152584	A. tetradius CCUG 47145
153114	A. tetradius CCUG 48516
156035	A. tetradius CCUG 60143 B
156691	A. tetradius CCUG 64164

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
123489	positive	coccus-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
1259	PYG MEDIUM (MODIFIED) (DSMZ Medium 104)	Medium recipe at MediaDive	Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
1259	CHOPPED MEAT MEDIUM (DSMZ Medium 78)	Medium recipe at MediaDive	Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) K2HPO4 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l (optional) Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water
40869	MEDIUM 6 - Columbia agar with 10 % horse blood		Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
123489	CIP Medium 20	Medium recipe at CIP
123489	CIP Medium 6	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
1259	positive	growth	37
40869	positive	growth	37
67770	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
1259	anaerobe
123489	anaerobe
125438	anaerobe	90.304
125439	obligate anaerobe	99.368

Spore formation

@ref	Spore formation	Confidence
125439		95.408

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68380	16024 ChEBI	D-mannose	+	fermentation	from API rID32A
68380	29985 ChEBI	L-glutamate	-	degradation	from API rID32A
68380	17632 ChEBI	nitrate	-	reduction	from API rID32A
123489	17632 ChEBI	nitrate	-	reduction
123489	16301 ChEBI	nitrite	-	reduction
68380	16634 ChEBI	raffinose	-	fermentation	from API rID32A
68380	27897 ChEBI	tryptophan	-	energy source	from API rID32A
68380	16199 ChEBI	urea	-	hydrolysis	from API rID32A

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68380	35581 ChEBI	indole		from API rID32A
123489	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68380	35581 ChEBI	indole	-	from API rID32A

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68380	alanine arylamidase	-	3.4.11.2	from API rID32A
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68380	alkaline phosphatase	-	3.1.3.1	from API rID32A
68380	alpha-arabinosidase	-	3.2.1.55	from API rID32A
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68380	alpha-fucosidase	-	3.2.1.51	from API rID32A
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68380	alpha-glucosidase	+	3.2.1.20	from API rID32A
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68380	beta-galactosidase	-	3.2.1.23	from API rID32A
68380	beta-Galactosidase 6-phosphate	-		from API rID32A
68382	beta-glucosidase	-	3.2.1.21	from API zym
68380	beta-glucosidase	-	3.2.1.21	from API rID32A
68382	beta-glucuronidase	+	3.2.1.31	from API zym
68380	beta-glucuronidase	+	3.2.1.31	from API rID32A
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68380	glutamate decarboxylase	-	4.1.1.15	from API rID32A
68380	glutamyl-glutamate arylamidase	-		from API rID32A
68380	glycin arylamidase	-		from API rID32A
68382	leucine arylamidase	-	3.4.11.1	from API zym
68380	leucine arylamidase	-	3.4.11.1	from API rID32A
68380	leucyl glycin arylamidase	-	3.4.11.1	from API rID32A
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68380	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API rID32A
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68380	phenylalanine arylamidase	-		from API rID32A
68380	proline-arylamidase	-	3.4.11.5	from API rID32A
68380	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32A
68380	serine arylamidase	-		from API rID32A
68382	trypsin	-	3.4.21.4	from API zym
68380	tryptophan deaminase	-	4.1.99.1	from API rID32A
123489	urease	+	3.5.1.5
68380	urease	-	3.5.1.5	from API rID32A
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123489	-	-	+	+	-	-	-	-	-	-	+	+	-	-	+	+	-	-	-	-

API rID32A

@ref	URE	ADH (Arg)	alpha GAL	beta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha GLU	beta GLU	alpha ARA	beta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	MNE	RAF	GDC	alpha FUC	Reduction of nitrateNIT	IND	PAL	L-arginine arylamidaseArgA	ProA	LGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	PyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
1259	-	+/-	not determinedn.d.	-	-	+	-	-	+	-	+	-	-	-	-	-	-	+/-	-	-	-	-	-	+/-	-	-	+/-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host Body-Site	#Urogenital tract	#Vagina
#Host	#Human	#Female

Isolation

@ref	Sample type	Host species	Isolation date
1259	human vagina	Homo sapiens
67770	Vaginal discharge
123489	Human, Vagina	Homo sapiens	1979

Taxonmaps

69479

Global distribution of 16S sequence LC036320 (>99% sequence identity) for Anaerococcus tetradius subclade from Microbeatlas

Aquatic Samples	44
Soil Samples	86
Animal Samples	8492
Plant Samples	14
Total Samples	8636

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Biosafety level	Biosafety level comment
1259	yes, in single cases	1	Risk group (German classification) According to TRBA 466
123489		1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM15909v1 assembly for Anaerococcus tetradius ATCC 35098	scaffold	GCA_000159095	525255.3	643886055	525255	51.15

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
1259	Anaerococcus tetradius gene for 16S ribosomal RNA, partial sequence, isolate: GIFU 7672	D14142	1377		33036
67770	Anaerococcus tetradius gene for 16S ribosomal RNA, partial sequence, strain: JCM 1964	LC036320	1487		33036

GC content

@ref	GC-content (mol%)
1259	30.0
67770	30

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM15909v1 assembly for Anaerococcus tetradius ATCC 35098
NCBI: GCA_000159095

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate anaerobe	99.37	no
125439	motility	BacteriaNetⓘ	no	65.47	no
125439	gram_stain	BacteriaNetⓘ	positive	52.49	no
125439	spore_formation	BacteriaNetⓘ	no	95.41	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Anaerococcus tetradius ATCC 35098
PATRIC: 525255.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	83.89	no
125438	anaerobic	anaerobicⓘ	yes	90.30	yes
125438	spore-forming	spore-formingⓘ	no	76.07	no
125438	aerobic	aerobicⓘ	no	98.01	yes
125438	thermophilic	thermophileⓘ	no	91.88	no
125438	flagellated	motile2+ⓘ	no	87.98	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	The gene expression and bioinformatic analysis of choline trimethylamine-lyase (CutC) and its activating enzyme (CutD) for gut microbes and comparison with their TMA production levels.	Ramireddy L, Tsen HY, Chiang YC, Hung CY, Chen FC, Yen HT.	Curr Res Microb Sci	10.1016/j.crmicr.2021.100043	2021
Metabolism	Identification of substrate-binding and selectivity-related residues of maltooligosyltrehalose synthase from the thermophilic archaeon Sulfolobus solfataricus ATCC 35092.	Tseng WC, Lin CR, Hung XG, Wei TY, Chen YC, Fang TY.	Enzyme Microb Technol	10.1016/j.enzmictec.2014.01.003	2014
Metabolism	Engineering of Fatty Acid Synthases (FASs) to Boost the Production of Medium-Chain Fatty Acids (MCFAs) in Mucor circinelloides.	Hussain SA, Hameed A, Khan MAK, Zhang Y, Zhang H, Garre V, Song Y.	Int J Mol Sci	10.3390/ijms20030786	2019
Enzymology	Identification of the essential catalytic residues and selectivity-related residues of maltooligosyltrehalose trehalohydrolase from the thermophilic archaeon Sulfolobus solfataricus ATCC 35092.	Fang TY, Tseng WC, Shih TY, Wang MY.	J Agric Food Chem	10.1021/jf073320b	2008
Metabolism	TreX from Sulfolobus solfataricus ATCC 35092 displays isoamylase and 4-alpha-glucanotransferase activities.	Park HS, Park JT, Kang HK, Cha H, Kim DS, Kim JW, Park KH.	Biosci Biotechnol Biochem	10.1271/bbb.70016	2007
Enzymology	Acceptor specificity of trehalose phosphorylase from Thermoanaerobacter brockii: production of novel nonreducing trisaccharide, 6-O-alpha-D-galactopyranosyl trehalose.	Maruta K, Watanabe H, Nishimoto T, Kubota M, Chaen H, Fukuda S, Kurimoto M, Tsujisaka Y.	J Biosci Bioeng	10.1263/jbb.101.385	2006
Metabolism	Characterization of the trehalosyl dextrin-forming enzyme from the thermophilic archaeon Sulfolobus solfataricus ATCC 35092.	Fang TY, Hung XG, Shih TY, Tseng WC.	Extremophiles	10.1007/s00792-004-0393-4	2004
Enzymology	Gene encoding a trehalose phosphorylase from Thermoanaerobacter brockii ATCC 35047.	Maruta K, Mukai K, Yamashita H, Kubota M, Chaen H, Fukuda S, Kurimoto M.	Biosci Biotechnol Biochem	10.1271/bbb.66.1976	2002
Metabolism	Genome mining for radical SAM protein determinants reveals multiple sactibiotic-like gene clusters.	Murphy K, O'Sullivan O, Rea MC, Cotter PD, Ross RP, Hill C.	PLoS One	10.1371/journal.pone.0020852	2011
	Molecular and virulence characteristics of an outer membrane-associated RTX exoprotein in Pasteurella pneumotropica.	Sasaki H, Ishikawa H, Sato T, Sekiguchi S, Amao H, Kawamoto E, Matsumoto T, Shirama K.	BMC Microbiol	10.1186/1471-2180-11-55	2011
Genetics	Comparison of the terrestrial cyanobacterium Leptolyngbya sp. NIES-2104 and the freshwater Leptolyngbya boryana PCC 6306 genomes.	Shimura Y, Hirose Y, Misawa N, Osana Y, Katoh H, Yamaguchi H, Kawachi M.	DNA Res	10.1093/dnares/dsv022	2015
Pathogenicity	In vaginal fluid, bacteria associated with bacterial vaginosis can be suppressed with lactic acid but not hydrogen peroxide.	O'Hanlon DE, Moench TR, Cone RA.	BMC Infect Dis	10.1186/1471-2334-11-200	2011
Enzymology	Abilities of the mCP Agar method and CRENAME alpha toxin-specific real-time PCR assay to detect Clostridium perfringens spores in drinking water.	Maheux AF, Berube E, Boudreau DK, Villeger R, Cantin P, Boissinot M, Bissonnette L, Bergeron MG.	Appl Environ Microbiol	10.1128/aem.02791-13	2013
Metabolism	Reactivation of latent HIV-1 by a wide variety of butyric acid-producing bacteria.	Imai K, Yamada K, Tamura M, Ochiai K, Okamoto T.	Cell Mol Life Sci	10.1007/s00018-012-0936-2	2012
	Further Identification of Novel Lantibiotic Operons Using LanM-Based Genome Mining.	O'Sullivan O, Begley M, Ross RP, Cotter PD, Hill C	Probiotics Antimicrob Proteins	10.1007/s12602-011-9062-y	2011
Genetics	Megasphaera vaginalis sp. nov. and Anaerococcus vaginimassiliensis sp. nov., new bacteria isolated from vagina of French woman with bacterial vaginosis.	Bordigoni A, Lo CI, Yimagou EK, Diop K, Nicaise B, Raoult D, Desnues C, Fenollar F.	New Microbes New Infect	10.1016/j.nmni.2020.100706	2020

References

#1259	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2951
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#40869	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68380	Automatically annotated from API rID32A .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#123489	Collection of Institut Pasteur ; Curators of the CIP; CIP 103927
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Anaerococcus tetradius (DSM 2951, ATCC 35098, CCM 3634)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API rID32A

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000159095

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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